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Citations for Roche :
3551 - 3600 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Indexing PCR was performed to add Nextera index adapters (1 μM final, Integrated DNA Technology) using KAPA HiFi reagents (#KK2102, Roche). Libraries were pooled in equal volumes and a final 0.8x cleanup was performed with homebrew SeraMag beads before measuring the sample concentration and sizing ...
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bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries with were multiplexed at equimolar concentration and sequenced on the Illumina HiSeq 2500 for 1×40 bases ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... Size selection using SPRI-beads isolated low molecular weight DNA which was then PCR amplified using 2 × HiFi HotStart Ready mix (Roche Diagnostics) and Nextera Indexing Primers (Illumina ...
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bioRxiv - Microbiology 2020Quote: All PCR reactions mentioned in this section were performed using KAPA HiFi DNA Polymerase (Hot Start and Ready Mix formulation) (Roche, Switzerland). Genes of interest were disrupted using a variation of the methods described previously (76) ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Cell Biology 2022Quote: ... Two different HRMA-compatible platforms were used (Applied Biosystems ViiA 7 Real-Time PCR System, using the MeltDoctor HRM Master Mix, and Roche LightCycler 480 System ...
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bioRxiv - Microbiology 2022Quote: Total genomic DNA was extracted from the bacterial pellet as described previously [28] using the PowerFood™ Microbial DNA Isolation kit (MoBio Laboratories Inc., Carlsbad, USA) and the High Pure PCR Template Preparation kit (Roche Diagnostics Ltd ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the cDNA samples were amplified using LightCycler® 480 SYBR® Green 2x PCR Master Mix I (Roche, Cat# 04887352001) and 0.6 μM of forward and reverse primers with a LightCycler® 480 (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche, Madison, WI, USA). The primers were as follows ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Genomics 2020Quote: ... Library quantification was performed using the Qubit dsDNA High Sensitivity kit and quantitative PCR with the KAPA Quantification kit (Roche #KK4854) on a QuantStudio 5 real-time PCR system.
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Immunology 2019Quote: ... and each of the three nested PCR steps was carried out in a total volume of 10 µL using 1 µL cDNA/PCR template and KAPA HiFi HotStart ReadyMix (Kapa Biosystems). In the last PCR reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 µg of linearized pCR©II-Tc-mef2 or pCR©II-Tc-duf/kirre was in vitro transcribed utilizing the DIG RNA labelling kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... PD-1 C192S mutant was generated by site-directed mutagenesis PCR reaction using platinum PWO SuperYield DNA polymerase (Roche, Basel, Switzerland) according to the product manual ...
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bioRxiv - Physiology 2019Quote: ... The reverse transcription product was diluted according to the efficiency curve and submitted in duplicates to real-time quantitative PCR using LightCycler® 480 SYBR Green I Master (Roche) in 7500 System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out in 12 μL volumes containing 6 μL of High Resolution Melting Master (Roche Applied Science, Germany), 0.24 μL of each 10 μM primer ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative reverse transcription PCR was performed using Eppendorf Realplex2 Mastercycler with reaction mixtures prepared with KAPA SYBR Fast Universal qPCR Kit (KAPA Biosystems). The PCR primers for KIF1A ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Genomics 2021Quote: ... The captured product was split into two portions and both portions were amplified independently for 19 PCR cycles with KAPA HiFi HotStart ReadyMix (Roche Sequencing). The two amplified capture products for each sample were combined and purified with the MinElute PCR Purification Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... All PCR reactions were performed using SYBR-containing master mix from the KAPA Real-Time Library Amplification Kit (Kapa Biosystems #KK2702) and terminated in the mid-exponential phase to limit over-amplification ...
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bioRxiv - Immunology 2021Quote: ... variable region) was used as template in a 25 µl PCR reaction with 2× KAPA HiFi Hot Start Ready Mix (Roche #KK2602) and 1 µl (10 µM ...
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bioRxiv - Neuroscience 2020Quote: ... Results were obtained with the Basic Relative Quantification Analysis of the LightCycler® 480 Real-Time PCR System software (Roche, Switzerland) and analyzed using the ΔΔCt method ...
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bioRxiv - Biochemistry 2021Quote: ... The expression of targeted genes and GAPDH transcripts were monitored by LightCycler®480 Real-time PCR (Roche Diagnostics GmbH, Germany) using gene-specific primers (Eurofins ...
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bioRxiv - Plant Biology 2021Quote: To analyse the relative gene expression of genes related to flavonoid biosynthesis in response to abiotic stress treatment a qRT-PCR was performed on a LightCycler® 480 II (Roche Diagnostics Deutschland GmbH ...
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bioRxiv - Plant Biology 2021Quote: ... The purified PCR product was then used for an in vitro transcription reaction using the DIG RNA Labeling Mix (#11175025910, Roche, Germany) and T7 and Sp6 RNA polymerase to generate both sense and antisense probes ...
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bioRxiv - Plant Biology 2021Quote: ... a digoxigenin-labeled probe corresponding to 415 bp of the ACMV AC1 gene was generated using a PCR DIG Probe Synthesis kit (Roche Diagnostics) and the primer pair ...
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bioRxiv - Microbiology 2019Quote: The extracted genomic DNA from passaged cultures was prepared for leader-end array sequencing by performing a 20-cycle PCR using Kapa HiFi Polymerase (Kapa Biosystems) and the primers listed in Table S3 ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). For PCR1 ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Plant Biology 2020Quote: ... were labelled by PCR in the presence of digoxygenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics) and biotin-16-dUTP (Roche Diagnostics ...
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bioRxiv - Zoology 2021Quote: ... a fragment of the EHP small subunit ribosomal RNA (SSU rRNA) gene was used to generate an EHP probe with a PCR-DIG labeling kit (Roche, Germany) ENF779 and ENR779 (Tangprasittipap et al. ...
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bioRxiv - Pathology 2021Quote: ... The obtained individual libraries were also quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit and an equimolecular pool of libraries were titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler 480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Cancer Biology 2022Quote: NGS libraries were prepared from extracted gDNAs following a 2-step PCR protocol with 2 x KAPA Mastermix (KK2612, KAPA Biosystems). For spleen Tregs and CD4s ...
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bioRxiv - Microbiology 2021Quote: ... The final concentration of the DNA library was quantified with real time PCR using the Kapa library quantification kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... for 40 cycles at 95°C for 5 s and 60°C for 30 s on a Light Cycler 480 Real-Time PCR System (Roche, Switzerland). All primer sequences used in this study are listed in Supplementary materials (Supplementary Table S1).
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bioRxiv - Molecular Biology 2020Quote: ... Poly-A enriched libraries were generated using the Illumina TruSeq Standard mRNA preparation kit and checked via the Bioanalyzer and quantitative PCR (KAPA Biosystems). Paired-end reads (100 nt ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Indexed libraries (that met appropriate cut-offs for both) were quantified by qRT-PCR using a commercially-available kit (KAPA Biosystems), and insert size distribution was determined using a LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... the PCR products digested with indicated restriction enzymes and ligated into the pBAD28 vector using the Rapid DNA Ligation Kit (Roche Diagnostics). Inserted DNA sequences were confirmed by DNA sequencing (StarSeq).
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bioRxiv - Immunology 2020Quote: ... ATAC-Seq libraries were then purified using a QIAGEN PCR cleanup kit and quantified using KAPA library quantification kit (KAPA Biosystems, Roche) and sequenced on the Nextseq 550 platform.
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bioRxiv - Cancer Biology 2021Quote: ... This was followed by 20 cycles of polymerase chain reaction (PCR) amplification using KAPA HiFi HotStart ReadyMix (#KK2602, Roche, Basel, Switzerland) and subsequent purification with Agencourt AMPure XP beads as described ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by Real-Time PCR on Light Cycler II using Light Cycler 480 SYBR Green I Master (Roche, 04887352001).
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bioRxiv - Evolutionary Biology 2022Quote: ... were ligated to the mate pair fragments and 8 cycles of PCR was used to enrich for the final library (KAPA Biosystems). The prepared libraries were quantified using KAPA Biosystems’ next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Synthetic Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed into cDNA and then quantitative real-time PCR was performed using a LightCycler 96 System (Roche, Switzerland). Relative gene expression was calculated using the 2-ΔΔCt method after normalizing to GAPDH expression ...
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bioRxiv - Molecular Biology 2019Quote: ... and then homology to the plasmid AatII site. Linear plasmids were amplified from circular plasmids (Fig. 2A) by two-step PCR using Kappa HiFi HotStart polymerase (Roche, Swtizerland). Cycling conditions were ...