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Citations for Roche :
3351 - 3400 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free library was prepared following the Kapa Hyper Prep Kit procedure provided by Roche (Roche, Basel, Switzerland). The library was sequenced on a paired-end mode with an Illumina NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Physiology 2024Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche). The final libraries were quantitated with Qubit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR reactions used a THUNDERBIRD SYBR qPCR Mix (TOYOBO) and were amplified on a LightCycler 480 System II (Roche). The specific primer pairs used for qRT-PCR analyses were designed using the Primer Express version 2.0 software program (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... Relative quantification analysis was performed using the LightCycler® 480 Real-Time PCR System software (v1.5.0 SP3, Roche Applied Science). Efficiency values for each amplicon were calculated by LinReg PCR software (Ramakers et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The let-A and I1B fragments were amplified from S2 cell genomic DNA using the Expand Long Template PCR System (Roche) and cloned in pBacPAK8_EGFP under a metallothionein promoter ...
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bioRxiv - Immunology 2021Quote: ... Pre-amplification of the V3V4 region of 16S rRNA was performed by PCR in triplicate using 2xKAPA HiFi Hot Start ReadyMix (Roche) using primer pairs containing adaptor sequences ...
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bioRxiv - Microbiology 2020Quote: ... Both the pET28a vector and the Rv1630 gene amplification product (1446 bp) were purified using the PCR quick spin kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... for genes of interest and Gapdh (a normalization control for amplification efficiency) on a LightCycler 480 Real-Time PCR machine (Roche). The primer sequence used for qPCR analysis is listed in Supplementary Table 12 ...
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bioRxiv - Developmental Biology 2021Quote: ... We used a 7900HT Fast Real-Time PCR System with 384-Well Block Module (Applied Biosystem) with Faststart universal SYBR Green Master (rox) (FSUSGMMRO Roche). The relative fold change for each gene expression was calculated by comparing treated and untreated conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Kapa Hifi HotStart polymerase using conditions recommended for a <500 base pair (bp) product (Kapa Biosystems, Roche Molecular Systems ...
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bioRxiv - Genomics 2020Quote: ... The PCR reaction contained 5 μl of 2X Kappa Hifi Hotstart ReadyMix (Kappa Biosystems, Roche Sequencing and Life Science, USA), 2 μl of 0.2 mg/ml BSA ...
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bioRxiv - Genomics 2020Quote: ... The PCR reaction contained 12.5 μl of 2X Kappa Hifi Hotstart ReadyMix (Kappa Biosystems, Roche Sequencing and Life Science, USA), 1 μl each of 5 μM forward and reverse primer ...
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bioRxiv - Pathology 2022Quote: ... We used genomic DNA isolated from ear tissue with a High Pure PCR Template Preparation Kit (Roche Diagnostics, Basel, Switzerland). The transgene was detected using RT2 qPCR Primer Assays from Qiagen (Venlo ...
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bioRxiv - Genetics 2022Quote: ... 10 µl of each PCR product were run in 2% agarose gels alongside digoxigenin (DIG)-labeled size markers VII and VIII (Roche), for 16 hours at 50 V then transferred to a positively charged nylon membrane (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Real time PCR for tuft cell markers and antimicrobial peptide genes was performed using Fast Start Universal SYBR Green Master (Roche) and ViiA™7 Real-Time PCR System (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Molecular Biology 2021Quote: ... Circular RNA levels were quantified by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), using Sybr Green mix (dssTakara, Japan) and detected by Lightcycler LC 480 (Roche). Primer sequences for qRT-PCR have been summarised in Supplementary Table 2.
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bioRxiv - Molecular Biology 2020Quote: ... We then used four times fewer cycles than indicated by the qPCR to lead to logarithmic phase of amplification and prepared a PCR reaction of 50 µl PCR master mix consisting of 25µl of 2x KAPA HiFi Uracil+ hot start polymerase (Roche # KK2800) mix ...
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bioRxiv - Developmental Biology 2022Quote: ... and the complementary DNA (cDNA) obtained was used for real time quantitative PCR using LightCycler instrument (Roche Life Science, Germany). cDNA was quantified using TaqMan Gene expression assays and the TaqMan primers to target genes were purchased from Thermo Fisher Scientific listed in Table 1 ...
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bioRxiv - Genomics 2022Quote: ... The libraries were quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay for size and the concentration was estimated using quantitative PCR with the KAPA Library Quantification Kit Illumina Platforms (Roche).
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bioRxiv - Genomics 2020Quote: ... Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche) in a total volume of 16µl ...
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bioRxiv - Genomics 2020Quote: ... The optimal numbers of PCR cycles for individual libraries were determined with a Real-Time Library Amplification Kit (KAPA Biosystems) by preliminary qPCR-based quantification using an aliquot of adaptor-ligated DNAs as described previously24 ...
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bioRxiv - Genomics 2020Quote: ... Illumina adapters and indices were added in the same one-step PCR reaction using the KAPA HiFi HotStart ReadyMix (Roche). The PCR product was purified using the QIAQuick PCR Purification Kit (Qiagen ...
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis and PCR amplification were carried out in the same tube using the qScript One-Step SYBR Green qRT-PCR Kit (Quantabio) and a LightCycler480 II (Roche). All reactions were performed in triplicate ...
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bioRxiv - Microbiology 2019Quote: ... RNA transcripts of the cloned 5’UTRB7 fragments were produced in vitro from the resulting purified PCR products by using the T7 Transcription kit (Roche) and labeled by using the RNA 3’ end biotinylation kit (Pierce) ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA probes were generated using PCR with T7 promoter sequence linkers and subsequently transcribed [DIG or FITC labeled] using T7 polymerase (Roche). Primer sequences are all found in Table 3 ...
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bioRxiv - Microbiology 2019Quote: ... and quantitative PCR (qPCR) undertaken on the complementary DNA (cDNA) with LightCycler 480 system using LightCycler 480 SYBR Green Master I (Roche). For the experiments displayed in Fig ...
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bioRxiv - Genomics 2021Quote: ... were used to prepare single-end (SE) libraries with an insert size of 300 bp using a PCR-free workflow with the KAPA HyperPrep Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Quantitative PCR experiments were performed with dsDNA fluorescent detection method using the FastStart Universal SYBR Green Master from Roche (04913850001).
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bioRxiv - Cancer Biology 2021Quote: ... The candidate cells were selected with 2 µg/ml puromycin for a week and the selected cells were analysed by High-resolution melting peaks analysis using real-time PCR (Light cycler 480 II, Roche).
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bioRxiv - Immunology 2021Quote: ... Relative gene expression of RORγt and FOXP3 was performed using Real Time PCR (Lightcycler 480, Roche Molecular System, Pleasanton, CA), SYBR Green and primers specific to RORγt ...
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bioRxiv - Genomics 2020Quote: ... 50 ng cDNA/reaction was used as a template in every 50 μL PCR reaction using Kapa Hifi hotstart ready mix (Roche). PCR conditions ...
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bioRxiv - Plant Biology 2020Quote: ... The second index was added to the amplified libraries through a second PCR containing 12.5 μl KAPA HiFi Hotstart ReadyMix (Kapa biosystems), 1.25 μl 5 μM p7 primer (Supplemental Table 3) ...
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bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Real time quantitative PCR was performed on the E gene as described recently (Corman et al., 2020) on a Lightcycler LC480 (Roche).
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bioRxiv - Biochemistry 2021Quote: ... PCR amplification and quantification were performed on a Roche LightCycler 480 using the SYBR Green I Master reaction mix (Roche). For each experiment ...
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bioRxiv - Neuroscience 2021Quote: ... Relative quantitative PCR assays were carried out using a LightCycler 480 and the SYBR Green I Master mix (Roche LifeScience), with Act5C as internal control for normalization of mRNA levels ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) analyses of cDNA (diluted at 1/10) were performed in triplicate with a LightCycler 480 II (Roche) using the SYBR Green I Master mix (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... and qPCR was performed with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne) or qPCRBIO SyGreen Mix Lo-ROX (PCR Biosystems Ltd) on LightCycler 96 (Roche). The levels of CNOT4 mRNA expression were used for normalization ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed using equal amount of cDNA and FastStart Universal SYBR Green Master (ROX) reagent (Roche Applied Science). Primer sequences used for mouse GSAP were described previously68 ...
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bioRxiv - Cancer Biology 2021Quote: ... A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche). Sequences of the primers used are listed in the reagents or resource table.
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bioRxiv - Cell Biology 2021Quote: ... The resulting cDNA was used as a template for qRT-PCR using 5× HOT FIREPol qPCR Mix (Solis BioDyne) on the LightCycler 96 System (Roche). Relative expression values were calculated with the 2-ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Plant Biology 2021Quote: ... The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche), 0.5 μM forward and reverse primer and water ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... The semiquantitative real-time PCR was proceeded with the faststart universal SYBR green master from Roche (Ref. 04913914001, lot. 11929100) using gene-specific primers ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Whole blood telomere lengths were measured using the monochrome multiplex quantitative PCR method (modified from (Cawthon, 2009) on a LightCycler 480 real time thermocycler (Roche). Briefly ...
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bioRxiv - Immunology 2022Quote: ... qPCRs were performed using specific primers for each gene (Supplementary Table 2) in Roche LightCycler 480 real-time PCR machine using Lightcycler 480 Sybr Green I Master kit (Roche). Actin was used as a reference gene (Supplementary Figure 1) ...