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Citations for Roche :
3401 - 3450 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and various concentrations of ADP-ribose or hit compounds were mixed on ice in 384-well PCR plate (Roche). Fluorescent signals were measured from 25 to 95 °C in 0.2 °C/30/Sec steps (excitation ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was performed with 2 x Hieff qPCR SYBR Green Master Mix (Yeasen) and detected by LightCycle 480 Real-Time PCR machine (Roche). For RNA-seq ...
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bioRxiv - Bioengineering 2022Quote: ... Libraries were barcoded with unique dual indexes to prevent index switching and adaptor-ligated double-stranded cDNAs were PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche). Final libraries were quantitated by Qubit (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained cDNA was used as template in a real-time quantitative PCR reaction using the SYBR Green master mix (Roche) and a LightCycler 1.5 (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, no. KK4610). Relative expression levels were normalized to human ACTB or mouse Actb ...
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bioRxiv - Microbiology 2020Quote: The PCR reaction system used the protocol of HiFi HotStart DNA Polymerase (Cat. No. KR0369, Kapa Biosystems, Boston, MA, USA), and the experiments were carried out based on a two-step PCR reaction ...
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bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
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bioRxiv - Plant Biology 2021Quote: ... The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, CA) to reduce the likeliness of multiple identical reads due to preferential amplification ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was immobilized using UV crosslinking (125 mJ/cm2) and hybridized at 42°C using Abion ULTRAhyb hybridization buffer and PCR DIG probes (Roche) synthesised according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... were carried out with the Applied Biosystems 7500 real-time PCR system with FastStart universal SYBR green master mix (Roche). All values were normalized to 18S RNA levels.
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR was performed using the KOD SYBR®qPCR Mix (Toyobo Life Science) with Light Cycler 480 II (Roche). Primers used to amplify the cDNAs are shown in Supplemental Table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... and quantitative real-time PCR was performed using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche). Oligonucleotides used for quantitative real-time OCR are listed in supplemental table S2 ...
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bioRxiv - Microbiology 2020Quote: ... and fractional recovery from the affinity selection step were assessed by quantitative PCR using Sybr Green I on a LightCycler thermal cycler (Roche). After five rounds of affinity maturation ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals) as recommended by the supplier ...
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bioRxiv - Bioengineering 2020Quote: ... and used as the DNA template for PCR reactions performed using KAPA HiFi HotStart DNA Polymerase (catalog no. KR0369; Roche Diagnostics Australia ...
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bioRxiv - Cancer Biology 2022Quote: ... 500-1000 ng of sheared DNA was used as a template for a 6-cycle PCR to construct a fragmented library using the KAPA HTP Library Preparation Kit (Roche). Exome enrichment was performed using SeqCap EZ Enrichment Kit (Roche ...
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bioRxiv - Immunology 2022Quote: ... pursued by 95°C for 3 min and then 45 cycles at 95°C for 15 sec and 58°C for 30 seconds using a LightCycler-480 Real-Time PCR system (Roche). The primers and the probes were designed against the E gene17.
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s protocol. Quantitative PCR was performed using SensiMixTM II Probe Kit (Cat#. BIO-83005) with Universal ProbeLibrary (Roche Diagnostics), and the CFX96 Touch System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Relative mRNA expression levels were determined using the SYBR Green I detection chemistry on LightCycler 480II Real-Time PCR System (Roche). The ribosomal constituent RPO was used as normalizing gene ...
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bioRxiv - Cell Biology 2022Quote: 2 μL of FLAG or IgG pull down and of a 1:10 dilution of the INPUT were used for amplification using qPCRBIO SyGreen Mix (PCR Biosystem) in a LightCycler96 (Roche) instrument ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were subsequently index-amplified for 20 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-μl reactions following the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR analysis was carried out using the ABI 7300 Real Time PCR System with Fast Start Universal SYBR Green Master Mix and ROX (Roche), and data were analyzed using the 2-ΔΔCT method ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR was performed with Thunderbird SYBR qPCR Mix (Toyobo, QPS-201) or KOD SYBR qPCR Mix (Toyobo, QKD-201) on a LightCycler 480 Real-Time PCR System (Roche). The relative expression level of each transcript was normalized by Gapdh expression levels ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified by gel electrophoresis and extracted from the gel via a commercial kit (HighPure PCR Product Purification Kit, Roche, DE). Sample preparation (Illumina TruSeq amplicon library ...
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bioRxiv - Genomics 2019Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems catalog no. KK4835). Libraries were then pooled and sequenced on the Illumina NextSeq 500 platform using Illumina sequencing reagents according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... The qRT-PCR assay was performed in triplicate with SYBR Green I Master reagent and the Light Cycler Nano system (Roche). Actin was used as the internal control for normalization ...
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bioRxiv - Molecular Biology 2019Quote: Quantitative PCR (qPCR) was performed for selected genes of interest (ERBB2 and reference genes) using a LightCycler 480 instrument (Roche). Each qPCR comprised 5 μl of the template cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... the USER digestion product was directly performed index PCR with 1 x KAPA HiFi HotStart Uracil+ ReadyMix (Kapa Biosystems, Cat.No.KK2801). Protocol of Strand Test 3 was almost same as Strand Test 2 ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCRs were performed in 96-well plates in a PCR thermal cycler (Applied Biosciences 7900HT) using a 2x FastStart TagMan Probe Mastermix assay (Roche) with a probe concentration of 100 nm and a primer concentration of 200 nm with the following primers ...
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bioRxiv - Plant Biology 2019Quote: ... The blots were hybridized with probes that were labeled using PCR DIG Probe Synthesis Kit and hybridization was detected with DIG Luminescent Detection Kit following the manufacturer’s instructions (Roche, Switzerland).
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bioRxiv - Cancer Biology 2019Quote: ... Five to ten PCR products were pooled equally and hybridized to a custom hereditary cancer panel (Roche NimbleGen, Madison, USA). Hybridization product was subsequently purified ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg of total RNA was subjected to reverse transcription with random primers using Takera’s TranscriptorFirst Strand cDNA Synthesis Kit and cDNA was subjected to real-time PCR analyses using LightCycler 480 SYBR Green I Master Mix (Roche) in a LightCycler 480 Instrument(BD ...
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bioRxiv - Systems Biology 2019Quote: The relative concentration of the resulting DNA ligated-probes was assessed by quantitative PCR (qPCR) using the LightCycler 480 SYBR Green I Master kit (Roche). 3.5 µl of diluted samples was used to assemble a 10 µl reaction and run on a Light Cycler 480 II (Roche ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The cDNA templates were then used for Sybr green real-time PCR to determine the transcription level of viral genes with a Light Cycler 480 system (Roche).
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bioRxiv - Immunology 2020Quote: The reaction mix per sample for quantitative real-rime PCR (qPCR) contained: 5 µl of the LightCycler 480 SYBR Green I Master Mix (04707516001, Roche), 2.5 µl cDNA and 2.5 µl primers in a total reaction volume of 10 ul ...
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bioRxiv - Plant Biology 2021Quote: ... A PCR master mix was prepared with the LightCycler 480 SYBR Green I Master Kit (Roche Applied Science, Mannheim, Germany). Each reaction was performed in a total volume of 10 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Titers of purified virus preparations were determined by quantitative PCR using a Roche Lightcycler 480 (Roche Applied Sciences, Pleasanton, CA) with primers amplifying the AAV2 ITR regions (forward ...
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bioRxiv - Epidemiology 2019Quote: ... The first round of PCR amplified the 73-V4 region using KAPA HIFI HotStart Ready Mix (Kapa Biosystems, Wilmington, MA) with following PCR conditions ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR reaction volumes of 100 nL consisted of 1 X LightCycler 480 SYBR Green I Master (Roche Diagnostics France), a cDNA sample of 1 ng.μL−1 ...
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bioRxiv - Genetics 2019Quote: ... was used as a template to generate fused promoter/gene products through overlap extension PCR using Expand Long Template polymerase (Roche) and PCR products were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Zoology 2020Quote: ... A 25 μl PCR master mix was prepared in a UV-sterilized hood and consisted of 3.125 μl Buffer (Kapa Biosystems), 1.25 μl dNTP (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... The final PCR reactions to produce Illumina-compatible sequencing libraries were prepared from the entire amplified cDNA using KAPA HiFi PCR Master mix (Roche) at 98 C for 2 min ...
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bioRxiv - Genomics 2021Quote: ... The enriched libraries were amplified by a second round of LM-PCR with 16 cycles using Kapa Hifi Uracil+ polymerase (Roche) and purified with AMPure XP magnetic beads ...
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bioRxiv - Genetics 2021Quote: ... each sample was amplified in 6/2 PCR reactions (2 µg DNA/reaction) in the primary/secondary screens using the ReadyMix Kapa polymerase (Roche) with a total of 20 cycles and an annealing temperature of 55°C (Primer sequences in Table S4 ...