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Citations for Roche :
3501 - 3550 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The LM-PCR products were then hybridized to the KAPA Target Enrichment Probes following the KAPA HyperCap Workflow v3.0 (Roche Diagnostics). To adjust this protocol for our cloning purposes ...
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bioRxiv - Microbiology 2024Quote: ... The dual barcodes were attached in a second PCR with the following reaction mix using KAPA HiFi HotStart ReadyMix (Roche), using 1 µL of a 1:10 dilution of the initial PCR as a template and 0.3 µM of the barcoding primers (Supplementary Table 2 ...
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bioRxiv - Microbiology 2024Quote: ... A two – step PCR protocol was used to prepare the sequencing library: a first amplification (target PCR) was carried out with 1X KAPA HiFi HotStart DNA polymerase (Roche), 0.3 µM of each 16S primer and 2 µL of template DNA ...
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bioRxiv - Cell Biology 2024Quote: ... The chromosome V was visualized with a biotin-labelled probe generated by amplifying the endogenous 5s rDNA locus by PCR and labelling was performed with the Biotin-nick translation mix (Roche) according with manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... with probe prepared by nick translation of a double-stranded PCR product bearing the bcd ORF using alkali-stable digoxigenin-11-2’-deoxyuridine-5’-triphosphate (Roche), visualizing with alkaline-phosphate coupled sheep anti-digoxigenin Fab fragments (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Eight replicate PCRs were carried out with 2.5 μg of gDNA and HiFi HotStart Ready Mix (Kapa Biosystems, Wilmington, MA). These PCRs were limited to 7 cycles to avoid PCR bias ...
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bioRxiv - Physiology 2024Quote: ... Gene expression was measured by quantitative reverse transcription PCR (qPCR) using LightCycler 480 SYBR Green I Master (50-720-3180 Roche). Quantitative polymerase chain reaction (PCR ...
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bioRxiv - Genetics 2024Quote: ... Libraries were quantified by real-time PCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Roche, Basel, Switzerland), followed by quality control using a DNA 100 Chip on a Bioanalyzer 2100 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The short insert paired-end libraries for the whole genome sequencing were prepared with PCR free protocol using KAPA HyperPrep kit (Roche). The library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2024Quote: ... or 3 µl of direct lysis extract were used as template in 50 µl PCR reactions using KAPA HiFi HotStart ReadyMix (Roche), primers P3/4 and P5 (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Cell Biology 2024Quote: ... The quantitative real-time PCR was done using Maxima SYBR Green/ROX qPCR Master Mix (2X) (Fermentas) and LightCycler 480 (Roche) quantitative PCR machine ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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Legionella relative abundance in shower hose biofilms is associated with specific microbiome membersbioRxiv - Microbiology 2023Quote: ... Two – step PCR protocol was used to prepare the sequencing library: a first amplification (target PCR) was carried out with 1X KAPA HiFi HotStart DNA polymerase (Roche), 0.3 µM of each 16S primer and 5 µL of 0.1 to 10 ng of template DNA ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10μl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on a QuantStudio 3 Real-Time PCR system ...
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bioRxiv - Genomics 2023Quote: ... and incorporation of sample-specific multiplex indices before PCR amplification (12 cycles) according to the manufacturer’s protocol (Kapa Biosystems, USA). Size distribution and molarity of amplified cDNA libraries were assessed via the Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: RU5 and Gag reverse transcripts and integrated HIV-DNA levels were quantified by nested real-time PCR (Light Cycler 480, Roche). Experiments were performed in triplicates on 0.5-1×105 T-cells/PCR ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative PCR was performed in a total of 10 μl on a LightCycler 480 thermal cycler (Roche Diagnostic, Mannheim, Germany) using PowerUp™ SYBR™ Green Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 0.2μM of specific primer pairs (Table S9) and run on a light cycler rapid thermal system (LightCycler®480 2.0 Real time PCR system, Roche) with 20 sec of denaturation at 95°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was performed using the 5x HOT FIREPol EvaGreen qPCR Supermix (Solis Biodyne) and the LightCycler 480 II instrument (Roche) as technical triplicates ...
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bioRxiv - Genomics 2023Quote: ... GenBank accession number Z69354.1) and BlSat1 (Hong Ha 2018) were labelled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng genomic DNA was used as a template for PCR amplification with KAPA HiFi HotStart ReadyMix (KAPA Biosystems, KK2602) and primer pairs containing an internal locus-specific region and an outer Illumina-compatible adaptor sequence ...
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bioRxiv - Biochemistry 2022Quote: ... A quantitative real-time-PCR analysis was performed with a Roche Light Cycler 480 System (Roche Applied Science, Penzberg, Germany) using SYBR Premix (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... the libraries were amplified by 12-rounds of indexing PCR (2X KAPA HiFi Hot Start Ready Mix, Roche, cat.no. KK2601). Library quality was assessed using Bioanalyzer DNA High Sensitivity chip (Agilent ...
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bioRxiv - Bioengineering 2023Quote: ... The construct was synthesized by GenScript® and the plasmid was used as template for PCR amplification (Kapa Hifi Hotstart Ready Mix, Cat#F-530-L, Roche). The PCR amplicon was purified with NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2023Quote: ... The 18S-1 probe contains a 552-bp fragment of the sugar beet 18S-5.8S-25S ribosomal RNA (rRNA) gene with the 35S rDNA (Schmidt et al. 1994) labeled with digoxigenin-11-dUTP by PCR (Roche) and the probe pXV1 for the 5S rRNA gene (Schmidt et al ...
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bioRxiv - Plant Biology 2023Quote: ... and the probe pXV1 for the 5S rRNA gene (Schmidt et al. 1994) labeled with biotin-11-dUTP by PCR (Roche). The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988 ...
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bioRxiv - Systems Biology 2023Quote: ... the entire eluate was used as input for a 300 μl PCR reaction mastermix with the KAPA HiFi HotStart DNA Polymerase kit (Roche Sequencing Solutions Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time quantitative PCR (qPCR) with the Kapa HiFi HotStart Library Amp kit (Roche, KK2612). For Illumina short-read sequencing ...
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bioRxiv - Immunology 2023Quote: ... and gene expression assessed using a BioMark HD (Fluidigm) according to the manufacturer’s instructions or a LightCycler® 480 Real-Time PCR System (Roche). The annealing temperature was 62°C ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was isolated by phenol-chloroform extraction and ethanol precipitation and analyzed by qPCR using Light Cycler Real-Time PCR System (Roche) and iTaq Universal SYBR Green Supermix SYBR Green Master Mix (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... quantitative real-time PCR was performed in duplicates in 384-well plates using Fast Start SYBR Green Master Rox (Roche) on a QuantStudio™ 5 real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... was used for cDNA synthesis and qRT-PCR was performed using FastStart Essential DNA Probes Master and gene specific probes from Universal Probe Library (Roche). Lightcycler 96 (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were amplified by ligation-mediated PCR for 6 cycles using a KAPA HiFi HotStart high-fidelity DNA polymerase (Roche) and purified using AMPure XP beads.
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed using qScript (Quanta Biosciences) followed by real-time PCR using KAPA SYBR FAST ROX Low (Roche) on the ViiA 7 Real-Time PCR instrument (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quantitative real time PCR was performed using the Luna Universal qPCR Mastermix (NEBiolabs) and the Light Cycler 480 system (Roche). Actin and eIF4A homologs were used as reference genes as established by Arai and collaborators (Arai et al ...
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bioRxiv - Bioengineering 2023Quote: ... Adaptor-ligated double-stranded cDNAs that were synthesized from the mRNA libraries were then PCR-amplified for eight cycles with KAPA HiFi DNA Polymerase (Roche), quantitated by PCR and subsequently pooled in equimolar concentration ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA concentration was measured on a Nanodrop spectrophotometer and 100 ng of DNA per sample was PCR-amplified with KAPA2G Robust HotStart ReadyMix (Roche) based on findings of low PCR bias for KAPA polymerase9394 ...
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bioRxiv - Bioengineering 2023Quote: ... The sequencing libraries were validated on the Agilent TapeStation 4200 and quantified by using Qubit 2.0 Fluorometer as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were multiplexed and clustered onto a flowcell ...
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bioRxiv - Immunology 2023Quote: ... The equally mixed pool of VH and Vκ/Vλ gene fragments was subjected to overlap extension PCR to generate scFv genes using a KAPA Biosystems kit (Roche) with overlap extension primers (No ...
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bioRxiv - Cancer Biology 2024Quote: ... donor sequences were cloned into a cloning vector and PCR amplification (25 cycles) was performed using Kapa-HiFi polymerase (Roche). DNA purification was performed by solid-phase reversible immobilization using AMPure XP (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed in a Corbett Rotor-Gene™ RG 6000 using Kapa SYBR®FAST (KAPA Biosystems) qPCR mix.
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bioRxiv - Developmental Biology 2024Quote: ... The real-time PCR was performed with Roche LightCycler 480 SYBR GREEN I Master mix on a LightCycler 480 system (Roche). Relative gene expression abundance between samples was calculated using the CT method and Gapdh as an internal control ...
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bioRxiv - Cancer Biology 2024Quote: ... The duplex UMI-tagged fragments were PCR amplified in a 50µl reaction volume (25µl KAPA® HiFi HotStart ReadyMix (Roche Diagnostics), 10µl IDT xGen™ unique dual index (UDI ...
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bioRxiv - Molecular Biology 2024Quote: ... Indexed libraries that met appropriate cutoffs for both were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the three different cell types were assayed for telomerase activity according to the TeloTAGGG Telomerase PCR ELISA Kit (11854666910; Roche).
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bioRxiv - Immunology 2024Quote: Pre-amplification of the V3/V4 region of 16S rRNA was performed by PCR in triplicate using 2xKAPA HiFi Hot Start ReadyMix (Roche) using primer pairs containing adaptor sequences for down-stream use on Illumina platforms ...
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bioRxiv - Systems Biology 2024Quote: The eluted samples were used as a template for a PCR reaction for amplifying Tn5 tagmented fragments and sample barcoding (2× Kappa HIFI [Roche] ...
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bioRxiv - Neuroscience 2024Quote: ... The vectors containing pegRNA and PE3/PE3b guides were used as template for PCR using KAPA HiFi HotStart DNA Polymerase with 2x Master Mix (Roche) to amplify parts containing the U6 promoter and either the pegRNA or PE3/PE3b guide using primers with BsaI recognition sites ...