Labshake search
Citations for Roche :
3651 - 3700 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and phoD-R1083 (5’-CTG SGC SAK SAC RTT CCA-3’) (Ragot et al., 2015) in 25 µL reactions with KAPA2G Robust HotStart PCR kit (KAPA Biosystems, Roche; 0.5 U KAPA2G Robust HotStart DNA Polymerase ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were validated using the Agilent TapeStation and quantified by using Qubit Fluorometer as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were multiplexed and clustered on a flowcell ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real time PCRs (qPCRs) were performed in a total volume of 10 µl with Kapa SYBR Fast qPCR kit (KAPA Biosystems) on an ABI 7500 fast machine operated with ABI 7500 software (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system ...
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bioRxiv - Genetics 2024Quote: ... qRT-PCR was performed in technical duplicate or triplicate using SYBR FAST qPCR Master Mix (Kapa Biosystems, Cape Town, South Africa) on a LightCycler 480 (Roche Applied Science ...
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bioRxiv - Developmental Biology 2024Quote: Pitx1 WISH was performed on E12.5 embryos with a digoxigenin-labelled Pitx1 antisense probe designed from a cloned antisense probe (PCR DIG Probe Synthesis Kit, Roche 11636090910). Experimental procedure followed the protocol outlined in (Kragesteen et al. ...
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bioRxiv - Genomics 2024Quote: ... Adapted fragments were amplified by 12 cycles of PCR using the Kapa Hifi Hotstart NGS library Amplification kit (Roche, Basel, Switzerland), followed by 0.8x AMPure XP (Beckman Coulter Genomics ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was cleaned using SPRIselect beads and 30 ng of cDNA is used as input for the PCR amplification (20 cycles) with Kapa HiFi HotStart Ready Mix (Roche Diagnostics) and addition of Illumina-index sequences ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 ug of the total RNA was reverse transcribed into cDNA and the quantitative real-time PCR was performed using a LightCycler 96 System (Roche, Switzerland). Relative gene expression was calculated using the 2−ΔΔCt method by normalizing it to GAPDH expression ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were performed using Phusion® Hot Start High-Fidelity DNA Polymerase (Finnzymes) or Expand High Fidelity DNA polymerase (Roche). Chromosomal DNA from PAO1 or KT2440 was normally used as DNA template in PCR reactions except for the amplification of the P ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed by amplifying 500 ng of total RNA using 9 PCR cycles with the KAPA mRNA HyperPrep Kit (KAPA Biosystems). cDNA libraries were validated using the Bioanalyzer 2100 on a High Sensitivity DNA assay and quantified using the KAPA Library Quantification Kit for Illumina Platforms on a Roche Lightcycler 480 ...
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bioRxiv - Genomics 2023Quote: ... was indirectly labeled by PCR in the presence of digoxigenin-11-dUTP detected by antidigoxigenin-fluorescein isothiocyanate (FITC; both from Roche Diagnostics). The remaining satDNAs (‘pTS5’ ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μg of gDNA from each sample was amplified in 20 individual PCR reactions using Kapa HiFi Master Mix (Kapa Biosystems) using TKOv3 library primers (forward ...
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids were linearized using a restriction enzyme digest and then amplified using inverse PCR with KAPA HiFi DNA polymerase HotStart ReadyMix (Kapa Biosystems). The inverse PCR product was treated with DpnI to digest the template DNA ...
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bioRxiv - Plant Biology 2023Quote: ... The expression patterns of candidate genes were explored by Real-time fluorescence quantitative PCR using a Roche thermocycler (LightCycler480, Roche, Switzerland). The ACTIN2 (At3g18780 ...
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bioRxiv - Cancer Biology 2023Quote: ... in addition to qPCR on the Applied Biosystems QuantStudio 5 Real-Time PCR System using the Roche KAPA Library Quantification Kit (Roche, KK4824) to determine the concentration of adapter-ligated libraries ...
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bioRxiv - Immunology 2023Quote: ... rs9500880 and rs1921622 was conducted by PCR utilizing fluorescent-labeled hybridization FRET probes followed by melting curve analysis in a LightCycler 480 (Roche Diagnostics). The following probes and primers were used ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed in duplicate for each sample using the LightCycler 480 Real-Time PCR System (F. Hoffmann-La Roche, Ltd.) with the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Developmental Biology 2023Quote: ... Indexing PCRs were done with 3 cycles less than the determined qRT-PCR cycle threshold (Ct) using KAPA HiFi HotStart ReadyMix (KAPA Biosystems) with final custom Illumina I7 and I5 concentrations at 1 μM ...
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bioRxiv - Systems Biology 2022Quote: ... CRISPR gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). In the first PCR step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We performed qRT-PCR with SYBR I Green Master Mix and a Roche LightCycler 480 (Roche Applied Science, Indianapolis, IN, USA) following the manufacturer’s protocol with triple technical replicates of 10 μl reactions (n = 20 for each parental context ...
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bioRxiv - Genomics 2023Quote: ... We ran indexing PCR on our NGS samples by creating the following mix per sample: 1x Kapa Hifi readymix HS (Roche, 7958935001), 0.5uM common primer (Table 1) ...
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bioRxiv - Zoology 2023Quote: The PCR products amplified from both IHHNV-309 and IHHNV-389 methods were used as templates to prepare digoxigenin (DIG)-labeled IHHNV probes using a PCR DIG-labeling kit (Roche, Germany). After preparation ...
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bioRxiv - Systems Biology 2023Quote: Strain-specific barcodes were extracted by PCR using the amplified whole transcriptome as template (1x KAPA HiFi Hotstart Readymix, Kapa Biosystems #KK2602 ...
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bioRxiv - Systems Biology 2023Quote: ... This entire volume was then used as template for an additional i5/i7 indexing PCR reaction (1x KAPA HiFi Hotstart Readymix, Kapa Biosystems #KK2602 ...
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bioRxiv - Neuroscience 2023Quote: ... An indexing PCR was performed to add Nextera index adapters (1 μM, Integrated DNA Technology) using KAPA HiFi reagents (Roche, #KK2102). Libraries were pooled in equal volumes and a final 0.8x cleanup performed with homebrew SeraMag beads before measuring sample concentration and quality ...
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bioRxiv - Neuroscience 2023Quote: ... sequencing primer binding sites and Illumina TruSeq indexes for multiplexing were introduced to the SAPseq miRNA and target libraries by PCR with the KAPA Hifi HotStart DNA polymerase (Roche KK2601). PCR products were purified using AmpureXP beads (Beckman A63880) ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription and second strand synthesis was performed on the slide with cDNA quantification using qRT-PCR using KAPA SYBR FAST-qPCR kit (KAPA Biosystems) and analysed on the QuantStudio (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2023Quote: ... 10ng input material obtained from the 10x cDNA purification (see section above) was used to perform library PCR using KAPA HiFi HotStart ReadyMix (Roche, KK2601) in 50µl reaction with the following steps ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were quantified using the Qubit BR dsDNA assay kit and equimolar pools were quantified by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems). Libraries were sequenced on the NovaSeq 6000 Illumina platform in 150PE mode ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each eluate (3 µl) was subjected to PCR amplification in 50-μl reactions containing 1 KAPA HiFi HotStart Uracil+ReadyMix (Kapa Biosystems) and 0.3 μM Dual Index primers of NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... 5 ng of DNA from each sample was analyzed using the Kapa Cyber Fast Q-PCR Kit (Kapa Biosystems, Wilmington, MA). The following rDNA primers (designed using Primer3Plus software ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were validated using the Agilent TapeStation and quantified by using Qubit Fluorometer as well as by quantitative PCR (KAPA Biosystems). The sequencing libraries were multiplexed and clustered on a flowcell ...
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bioRxiv - Cancer Biology 2024Quote: ... HSJD-DIPG-012 and HSJD-DIPG-014 cell lines by real-time quantitative QRT-PCR on a LightCycler® 480 instrument (Roche) using a RT² Profiler PCR Array (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of PCR cycle used for library amplification was determined by KAPA library quantification kit for Illumina platform (KAPA biosystems). Each Hi-C library was sequenced to obtain 500M reads PE100 with biological duplicates to achieve a total of 1 B reads for each condition.
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... library preparation for metagenomic analysis was conducted using the KAPA Hyper PCR-free library preparation kit (Kapa Biosystems, Roche, Basel, Switzerland), following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed to assess the concentration of target rRNA using SYBR Green Master Mix kit (Roche, Basel, Switzerland) in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 15 μL of a PCR mix with 1X LightCycler FastStart DNA Master Hybridisation Probes kit (Roche diagnostics, Mannheim, Germany), 0.5 U of UDG (Roche Diagnostics ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and an equimolecular pool of libraries was titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real time polymerase chain reactions (qRT-PCR) were performed in 20 μL reactions using the FastStart universal SYBR Green master (Rox) kit (Roche diagnostics Corp.) on an Mx3000P real-time PCR machine (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed in duplicates with 20 ng template cDNA per reaction using KAPA SYBR® FAST (KK4603, Roche, Basel, Switzerland) and a CFX96™ Real Time System C1000 Touch™ Thermal Cycler (BioRad ...
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bioRxiv - Physiology 2022Quote: ... was performed on the cDNA using the Sybr green method of quantification on a Roche Lightcycler 480 using a SYBR FAST quantitative PCR kit (Kapa Biosystems; KK4611). Gene expression was analyzed for each sample in triplicate using the forward and reverse primers indicated on Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems, catalog #KK4605). Primers (5’3’ ...
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bioRxiv - Cell Biology 2022Quote: ... Barcoded Illumina adaptor sequences (Table S2) were added to the tagmented DNA by PCR (KAPA HiFi HotStart ReadyMix, Kapa Biosystems, 12 cycles). And the DNA was cleaned with an x0.8 SPRI procedure ...