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Citations for Roche :
3301 - 3350 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Sequence libraries were created from enriched DNA using a 500 bp insert and standard PCR library amplification (KAPA Biosystems). Paired end sequences were obtained on either the Illumina Miseq or Nextseq platforms.
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bioRxiv - Microbiology 2022Quote: Quantitative Real-time PCR assays were performed with the KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems) on a LightCycler 1.5 (Roche ...
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bioRxiv - Microbiology 2022Quote: ... a 12.5 μl reaction/well was prepared in 4titude® 96-Well Semi-Skirted PCR Plates for LC480 (Roche) using a SuperScript III One-Step RT-PCR Kit (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... melting curve analysis was performed by scanning the PCR product in a LightCycler 480 Instrument II (Roche, Pleasanton, CA).
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bioRxiv - Neuroscience 2022Quote: ... and the pool was quantified by qRT-PCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems, Wilmington, USA) on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Whole genome amplification of HPV16 from oral cancer DNA was performed with an Expand High Fidelity PCR system (Roche) according to the manufacturer’s instructions using HPV type 16-specific primers ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were conducted by mixing 12.5 µL of the KAPA HiFi Hot Start Ready Mix (KAPA Biosystems, Wilmington, MA) with 0.4 µM of each primer (modified to include illumina adaptors) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was mixed with the respective primer pairs and SyGreen Blue Mix (PCR Biosystems) following the manufacturer’s protocol in biological triplicate and performed using a LightCycler 96 real-time PCR machine (Roche). Quantification cycle values were normalized to a reference gene (GAPDH ...
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bioRxiv - Immunology 2022Quote: ... The cDNA was enriched for knockin barcodes using a nested PCR strategy with Kapa HiFi HotStart Ready Mix (Roche) for 8 cycles per round ...
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bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
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bioRxiv - Molecular Biology 2024Quote: PCR-based amplification of sgRNA regions was performed as described previously27 with minor modifications - KAPA HiFi HotStart ReadyMix (Roche) was used for PCR1 and PCR2 with primers listed in Table S2 and following cycling conditions ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Neuroscience 2023Quote: ... In vitro transcription was performed with PCR-amplified templates using T7 RNA polymerase (Hoffman La Roche AG, Basel, Switzerland) to synthesise antisense RNA probes (Supplementary Table 29) ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplexed PCR reactions were purified using a 2X volume ratio of KAPA pure SPRI beads (KAPA Biosystems, Wilmington, MA). Purified PCR reactions were quantified using the Qubit dsDNA HS kit and Qubit 2.0 fluorometer ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplification of the first round of PCR was performed with the KAPA 2G Ready Mix (Roche Inc., Nutley, NJ) with the same PCR program as for TARGET-Seq47 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library quality was assessed with an Agilent 4200 Tapestation and Lightcycler quantitative PCR (Roche Life Science, St. Louis, MO) and then paired-end sequenced (2 × 150 bp ...
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bioRxiv - Genomics 2024Quote: ... Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche), primers P6 and P7 (Supplementary Table 2) ...
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bioRxiv - Biochemistry 2024Quote: ... The collected cDNA followed the same operation to complete the following qRT-PCR with SYBR Green (04913914001, Roche, Sweden) as the dye ...
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bioRxiv - Biophysics 2024Quote: ... A small fraction of the cDNA and input were allocated to real-time PCR quantification using a LightCycler 2.0 (Roche); the remainder was amplified by PCR ...
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bioRxiv - Bioengineering 2024Quote: ... The adaptor-ligated DNA was amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, Indianapolis, IN). The final libraries were quantitated on Qubit and the average size determined on the AATI Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was briefly pulsed in the centrifuge and sealed with a clear PCR plate seal (Roche Life Science). The LightCycler® instrument (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA amplification was performed in 50 µL PCR solution [1X Hifi Hot Start Ready mix (Kapa Biosystems, cat# KK2601), 1 µM SMRTpcr primer (Supplementary Table S6)] ...
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bioRxiv - Genomics 2023Quote: ... accession numbers OY726585 and OY726586) were indirectly labeled by PCR in the presence of biotin-16-dUTP (Roche Diagnostics) detected by streptavidin-Cy3 (Sigma–Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... was introduced by site directed mutagenesis on C-terminal SNAP-tagged H3.1 (HIST1H3C coding sequence cloned in pSNAPm) and H3.3 (H3F3B coding sequence cloned in pSNAPm) encoding plasmids with a PCR master kit (Roche) and the primers indicated in Supplementary table 2 ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions with a total volume of 25 μL included: 2× KAPA HiFi Hot Start Ready Mix (KAPA Biosystems), a final concentration of 0.1 mM for each primer and 7.5 μL of DNA sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... Real time PCR was performed on the cDNA using KAPA SYBR FAST qPCR Master Mix (2X) kit (Roche, #KK4618) with intron spanning primers in a QuantStudio 3 Real-time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 ng of cDNA was used for quantitative reverse transcription PCR with the FastStart SYBR Green Master (Roche) and the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of OsHV-1 and total bacteria were performed using quantitative PCR (qPCR, Roche LightCycler 480 Real-Time thermocycler) with the following program ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the molarity of the library was measured using quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR (qPCR) was performed following the manufacturer’s instruction using FastStart Essential DNA Green Master (Roche Molecular Systems, Switzerland). Target cDNAs were amplified using gene specific primer pairs (45S_FW ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Genomics 2023Quote: DNA extraction for sequencing with Illumina was done using High Pure PCR Template Preparation Kit from Roche (Mannheim, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid backbones and DNA fragments were amplified using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Boston, USA) and the primers described in Table S3 ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Whole genome amplification of HPV16 from oral cancer DNA was performed with an Expand High Fidelity PCR system (Roche) according to the manufacturer’s instructions using HPV type 16-specific primers ...
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bioRxiv - Biochemistry 2023Quote: ... Amplification reactions were performed in duplicate using a LightCycler 96 Real-Time PCR system (Roche Molecular Systems, CA, USA), and fluorescence curves were analyzed using this software ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification of the target area was performed using the 2X KAPA HiFi Hot Start Ready Mix (070988935001, Roche). Reverse and forward amplicon PCR primers recommended by Illumina were used (16s Amplicon PCR Forward Primer= TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG ...
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bioRxiv - Immunology 2023Quote: ... and the second-strand cDNA was synthesized using a KAPA Biosystems kit (Roche KAPA HiFi Hotstart PCR kit, KK2502) with VHgene-specific primers (No ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in 384-well plates with FastStart Universal SYBR Green Master Mix (04913914001, Roche) on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR product was used to synthesize in situ probe by the addition of DIG-labeled UTP (Roche) plus the appropriate RNA Polymerase T7 or Sp6 (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...
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bioRxiv - Immunology 2024Quote: ... Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform.
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bioRxiv - Cancer Biology 2024Quote: ... the captured DNA was PCR amplified in a 50µl reaction volume (25µl KAPA® HiFi HotStart ReadyMix (Roche Diagnostics), 2.5µl TWIST amplification primers ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Quantitative PCRs were performed using the KAPA SYBR qPCR Master Mix (SYBR Green I dye chemistry) (KAPA BIOSYSTEMS, KK4618) and the CFX connect apparatus (Bio-Rad) ...
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bioRxiv - Genomics 2024Quote: ... and 36B4 standard were calculated via Real-Time PCR method using the SYBR Green I Master mix (Roche, Germany), and telomere and 36B4 primers ...