Labshake search
Citations for Roche :
3201 - 3250 of 3852 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... SYBR Green (PB20.11-05, PCR Biosystems) was used on a real-time thermal cycler (LightCycler® 480, Roche). S16 or HPRT were used as internal control gene ...
-
bioRxiv - Cell Biology 2024Quote: All qPCR reactions were performed with a LightCyler 1.3 Real-Time PCR system (Roche S/N: 140 6143). The MgCl2 concentration (1-3 mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche). A set of 3 household genes has been used to normalize (Cyclophilin ...
-
bioRxiv - Genetics 2023Quote: ... mRNA expression was then analyzed by real-time PCR using KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems) and the CFX-96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
-
bioRxiv - Developmental Biology 2024Quote: ... and quantified by using Qubit 2.0 Fluorometer as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were multiplexed and clustered onto a flowcell on the Illumina NovaSeq instrument according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 72°C for 1 min) All PCR was performed using KAPA HiFi HotStart ReadyMix (Kapa Biosystems; KK2602). The PCR products were isolated by QIAquick PCR Purification Kit (Qiagen ...
-
bioRxiv - Cell Biology 2024Quote: ... The beads were washed four times with binding and washing buffer and treated with DNAse (PCR grade, Roche) for 10 minutes at 37 °C ...
-
bioRxiv - Evolutionary Biology 2024Quote: The PCR master mix (20 μL) that was prepared using KAPA HiFi HotStart Ready Mix (Roche, Basel, Switsland) (×2 KAPA HiFi PCRmix ...
-
bioRxiv - Cell Biology 2024Quote: ... The 1st strand cDNA was subjected to real-time PCR on a LC480 thermal cycler instrument II (Roche) using SYBR Premix Ex Taq (Tli RNaseH Plus ...
-
bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from mouse tail clippings and PCR amplified using the KAPA Mouse Genotyping Kit (Roche) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative PCR (qPCR) using a 96-well plate in a pre-heated LightCycler 480 Instrument II (Roche, Basel, Switzerland) was used to quantify the copy number of the 16S rRNA genes in the samples as previously described 117 ...
-
bioRxiv - Microbiology 2020Quote: ... whereas for site 15 we used 0.7 g of sediment per sample and diluted the final extracts 10 times in ultrapure PCR water (Roche). DNA templates were used in quantitative PCR (qPCR ...
-
bioRxiv - Zoology 2021Quote: ... PCR amplifications of the bacterial V3-V4 16S rRNA gene fragments were performed using KAPA HiFi HotStart ReadyMix (Roche) and the primer pair 341F (5’-CCTACGGGNGGCWGCAG-3’ ...
-
bioRxiv - Zoology 2021Quote: ... All PCR products were checked through electrophoresis on 1.5% agarose gel and then purified using KAPA Pure Beads (Roche) following the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix and the LightCycler® 480 instrument (Roche). Relative levels of gene expression were analyzed using the 2ΔΔCt method and compared to the expression of the human housekeeping gene PPIB ...
-
bioRxiv - Genetics 2021Quote: ... Real-time quantitative PCR (qPCR) was performed using SYBR Green Master Mix and the LightCycler® 480 instrument (Roche). Relative levels of gene expression were analyzed using the 2ΔΔCt method and compared to the expression of the human housekeeping gene PPIB ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche), primers P3 and P4 (Supplementary Table 1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2-μl aliquots of all cDNA samples were analyzed in triplicate on 96-well optical PCR plates (Roche Diagnostics). GAPDH or TBP was used as the reference gene and all analyses were performed using the ΔΔCt method with Roche LightCycler 96 system software ...
-
bioRxiv - Developmental Biology 2021Quote: ... tissues were dissected in ice cold 1x PBS and transferred to low binding PCR tubes containing 50-100µL of 1x Protease Inhibitor Cocktail in 1xPBS (cOmplete mini EDTA-free; Roche) on ice ...
-
bioRxiv - Developmental Biology 2021Quote: ... Pax3 digoxigenin (DIG) - labeled antisense riboprobes were transcribed from linearized gene-specific probes (PCR DIG probe Synthesis Kit, Roche). WISH experiment was performed as follows ...
-
bioRxiv - Cell Biology 2022Quote: ... qRT-PCR was performed with 1μl of the synthesized cDNA using LightCycler 480 SYBR Green I Master (Roche, 04707516001) on a QuantStudio 6 Flex instrument (Applied Biosystems ...
-
bioRxiv - Microbiology 2021Quote: ... The resultant cDNA was amplified by an adapter-based PCR using the KAPA HiFi DNA polymerase (Roche, Basel, Switzerland). Sequencing was performed using a MiSeq (Illumina ...
-
bioRxiv - Genomics 2019Quote: ... we performed qRT-PCR with Unique Aptamer™ qPCR SYBR® Green Master Mix (Novogene) on the RocheLightCycler480 (Roche) using the same samples used for next-generation sequencing to validate the alternative TES in different cell types ...
-
bioRxiv - Genomics 2020Quote: ... 5 ul of the three amplicons with 20 μl of PCR master mix containing 1.25X KAPA HiFi HotStart ReadyMix (Roche), 0.375 uM of the i5 primer (Microsynth AG ...
-
bioRxiv - Genomics 2020Quote: ... each PCR reaction was assembled by combining 5 ul of the pooled barcoded cDNA with 20 μl of PCR master mix (1.25X KAPA HiFi HotStart ReadyMix [Roche] ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems; Cat#KK4835). Libraries were then pooled and sequenced (1×75bp ...
-
bioRxiv - Molecular Biology 2020Quote: Some LAMP-OSD reactions were analyzed in real-time using LightCycler 96 real-time PCR machine (Roche, Basel, Switzerland). Reactions were subjected to 30 cycles of two-step incubations – step 1:150 sec at 65 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The reaction was carried out on a LightCycler® 96 Real-Time PCR system (Roche Applied Science, Indianapolis, USA). Internal control was the GAPDH gene while the 2-ΔΔCT method was used to assess expression ...
-
bioRxiv - Immunology 2019Quote: ... Amplification of cDNAs was performed by quantitative real-time PCR reactions on a Light Cycler instrument (LC480, Roche Diagnostics) with the Light Cycler 480 SYBR Green detection kit (Roche Diagnostics ...
-
bioRxiv - Developmental Biology 2019Quote: ... QPCR was performed on a 7900HT Fast Real-Time PCR System (Thermofischer) with SYBR green reagent (Roche, Cat#04707516001). The Rps9 cDNA was used for data normalization ...
-
bioRxiv - Microbiology 2019Quote: ... each DNA sample was amplified by polymerase chain reaction (PCR) using KAPA HiFi HS ReadyMix (KAPA Biosystems, Wilmington, MA) and primers specific for variable regions 3 and 4 of the 16S rRNA gene ...
-
bioRxiv - Genomics 2019Quote: ... Gene expression was measured by quantitative Real Time - Polymerase Chain Reaction (qRT-PCR) (LightCycler 480, Roche Diagnostics, Basel, Switzerland) using the SYBR Premix Ex Taq (Tli RNaseH ...
-
bioRxiv - Microbiology 2019Quote: Each real-time PCR reaction contained 1x Roche universal probe library (UPL) master mix (LightCycler 480 Probes Master, Roche), 80 nM UPL hydrolysis probe ...
-
bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR was performed with the LIght Cycler DNA Fast Start Master SYBR green I kit (Roche) in a Light Cycler 480 Instrument II at Tm=60°C ...
-
bioRxiv - Genetics 2021Quote: ... Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche), primers P3 and P4 (Table S1 ...
-
bioRxiv - Genetics 2021Quote: ... Sequencing adaptors and barcodes were added with a second round of PCR using the KAPA HiFi HotStart ReadyMix (Roche), primers P3 and P4 and 1 ng of template DNA ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified by precipitation and one microgram each was labeled by nick translation (Invitrogen or Roche Diagnostics) with Cy3-dUTP (GE ...
-
bioRxiv - Genomics 2021Quote: ... Then 100 nL of KAPA PCR mix (2.775 X HiFi Buffer, 0.85 mM dNTPs, 0.05 U KAPA HiFi polymerase / µL)(Roche Cat# 07958846001) was added to all wells containing a single nucleus via two 50 nL filtered dispenses ...
-
bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... The quantity of the libraries was measured by real-time PCR using the LightCycler® 480 Instrument II (Roche) and QIAseq™ Library Quant Assay Kit (Qiagen) ...
-
bioRxiv - Biochemistry 2021Quote: ... The thermal melting curve were monitored using a LightCycler 480 II Real-Time PCR System (Roche Diagnostics, Rotkreuz, Switzerland) with a ramp rate of 1 °C at the temperature range from 25 °C to 98 °C ...
-
bioRxiv - Cell Biology 2021Quote: Real-time PCR was performed using the LightCycler 480 SYBR Green and LightCycler 480 system (Roche Diagnostics, Mannheim, Germany), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The library amplification step included 6-7 PCR cycles and was performed using the KAPA Library Amplification kit (Roche). The final Hi-C library was purified using SPRIselect beads and the quality and size of the library (approximately 500 bp ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 5 times and subjected to real-time PCR using LightCycler 480 SYBR Green I Master (Roche) with specific primers for the genes of interest ...
-
bioRxiv - Microbiology 2021Quote: ... The 16S rRNA V3–V4 amplicon was amplified using a KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, USA). the amplicon PCR forward primer (5’-CCTACGGGNGGCWGCAG-3’ ...
-
bioRxiv - Microbiology 2021Quote: ... was used for the amplification and detection of cDNA targets on the LightCycler480 Real-time PCR system (Roche, USA). The mean cycle threshold (Ct ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
-
bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2020Quote: ... The first fragment was a diverse sample of inserts amplified via PCR using KAPA HiFi polymerase (Roche Basel, Switzerland), producing a sequence which contained random barcodes associated with a random sgRNAs from the library (with the appropriate reporter plasmid sequence connecting the two ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR analyses confirmed mouse genotypes with DNA isolated from ear biopsies using the KAPA Mouse Genotyping Kits (Kapa Biosystems). All PCR primers are listed in Supplementary Table 1.