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Citations for Lucigen :
1751 - 1800 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Transposon mutagenesis was performed with the EZ-Tn5
Tnp Transposome kit (Epicentre, Illumina, CA, USA) and the insertion site of all mutants was determined via arbitrary PCR (Segev et al ... -
bioRxiv - Microbiology 2021Quote: ... 40 and 50 U/μl of Ready-Lyse Lysozyme (Lucigen R1810M). qPCR was performed on the Thermo Fisher Quant Studio 3 Real-Time PCR machine with the following parameters ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from harvested cultures using the Masterpure™ Complete DNA and RNA Kit (Lucigen Corporation, Middleton, Wisconsin). RNA was subsequently purified using the Zymo RNA Clean and Concentrator™ Kit (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA extraction using QuickExtract DNA Extraction Solution (Lucigen) was performed according to manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted independently for each specimen using the MasterPure Complete DNA and RNA purification Kit (Epicentre) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al. ...
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bioRxiv - Microbiology 2021Quote: ... Residual Ribosomal RNA (rRNA) was depleted using Ribo-Zero™ kits (Epicentre, Madison, WI) before library construction ...
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bioRxiv - Microbiology 2021Quote: ... or C41(DE3) cells (Lucigen or Sigma) for plasmid propagation and protein expression ...
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bioRxiv - Microbiology 2021Quote: ... Total 5 μg of RNA was used for rRNA depletion by using Ribo-Zero™ (Epicentre, Illumina, Madison, WI USA) kit and purified by using Qiagen-RNeasy miniElute (Qiagen GmbH ...
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bioRxiv - Microbiology 2021Quote: ... a combined kit for the ribosomal (rRNA) depletion Ribo-Zero™ Kit (Bacteria) (Epicentre, Illumina, Madison, WI USA) and cDNA library construction kit ...
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bioRxiv - Microbiology 2021Quote: ... ScriptSeq™ v2 RNA-Seq library preparation kit (Epicentre, Illumina, Madison, WI USA) was used for this purpose ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq complete kit (Epicentre, Illumina, Madison, WI USA), a combined kit for the ribosomal (rRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... ScriptSeq v2 RNA-Seq library preparation kit (Epicentre Technologies, USA), TruSeq SBS Kit v3-HS (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
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bioRxiv - Molecular Biology 2021Quote: Eluted cDNA was moved to a new PCR tube and mixed with CircLigase II (Epicentre) reaction mix containing ...
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bioRxiv - Molecular Biology 2021Quote: ... Competent cells were electroporated with Ez-Tn5
transposome (Lucigen, Madison, WI). Cells recovered for 90 minutes at 30 °C and then played on PYE plates supplemented with kanamycin ... -
bioRxiv - Molecular Biology 2021Quote: ... 2.5 μL betaine (0.8 M; Lucigen, Middleton, WI), 1 μL MgSO4 (4 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... gDNA was removed using Baseline Zero DNase (Lucigen) following the manufacturer’s protocols and RNA reprecipitated using 0.1 volume of sodium acetate and 2-3 volumes 100% ethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted from one plate using QuickExtract DNA extraction solution (Epicentre) to perform genotyping by PCR using primer pairs specifically recognizing the mutated sequences introduced by the ssODN and a genomic sequence adjacent to the region of integration ...
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bioRxiv - Molecular Biology 2021Quote: ... the ssDNA was circularized with CircLigase (Epicentre) at 60 °C for 1h ...
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bioRxiv - Molecular Biology 2021Quote: ... were used to in vitro transcribe a 309-nt actin RNA transcript that was subsequently cap-labeled using recombinant VACV guanylyltransferase/guanine-7-methyltransferase (Epicentre Biotechnologies) in conjunction with capping buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genetics 2021Quote: ... 25 μM ATP standard solution (Epicentre) was used for preparing a concentration gradient (0 ...
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bioRxiv - Immunology 2021Quote: ... Monophosphorylated RNAs were selectively degraded by Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen) and RNAs were 5’dephosporylation by quickCIP (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from samples using a MasturePure extraction kit (Lucigen), and 16s rRNA amplicon libraries were prepared with primers 515F and 806R ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was prepared using the MasterPure™ Yeast DNA purification kit from Lucigen. To determine the MAT ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was subjected to rRNA-depletion using RiboZeroTM rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc. ...
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bioRxiv - Microbiology 2021Quote: ... ΔPfUPF1 and ΔPfUPF2 parasite cultures using QuickExtract (Lucigen). The identity of transfectant parasites were verified by PCR specific for the expected parental and modified loci ...
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bioRxiv - Immunology 2021Quote: Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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bioRxiv - Immunology 2021Quote: ... genomic DNA was isolated after 72 hours using QuickExtract (Lucigen, USA) and fragmented using the LOTUS™ DNA library kit (IDT ...
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bioRxiv - Microbiology 2021Quote: ... coli C43 (DE3) (Lucigen). Single colonies were used to inoculate 100 mL of Lysogeny Broth (LB ...
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bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... adding additional ATP and DNase every 24 h (30 units per day) according to the manufacturer’s protocol (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,). ecDNA-enriched samples were used as template for phi29 polymerase amplification reactions (150043 ...
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bioRxiv - Genomics 2021Quote: ... Remaining linear DNA was removed by exonuclease (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,) at 37 °C in a heating block and enzyme reaction was carried out continuously for 1 week ...
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bioRxiv - Genomics 2021Quote: ... and 5 electroporations were performed into Endura electrocompetent cells (Lucigen, Middleton WI) and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Genomics 2021Quote: ... 200 U of NxGen RNase Inhibitor (Lucigen), and protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2021Quote: ... and then were resuspended in 385 µL DNA Quick Extract (Lucigen cat# QE09050) and transferred to a 1.5 mL tube ...
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bioRxiv - Genomics 2021Quote: ... 11 μL purified CUT&Tag-DNA was used in the reaction (11 μL DNA, 2 μL 10 μM Tn5mC-ReplO1 oligo, 2 μL 10× Ampligase buffer (Lucigen), 2 μL dNTP mix (2.5mM each ...
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bioRxiv - Genomics 2021Quote: ... and 2.5 μL Ampligase (Lucigen) were added into each sample separately ...
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bioRxiv - Genomics 2021Quote: ... The library was transformed and amplified using 25 μl Endura Competent Cells (Lucigen) and 100 ng plasmid libraries per electroporation reaction as previously described24 ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using DNA QuickExtract (Lucigen), and qPCR was performed using iTaq Universal SYBR Green (Bio-rad ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Genomics 2021Quote: ... DNase-treated RNA was subjected to terminator digestion (TEX enzyme, Epicentre) to degrade 5’-monophosphate RNA (16S and 18S ribosomal RNA) ...
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bioRxiv - Genomics 2021Quote: Contents of a 25 µL PCR mixture included 12.5 µL of EconoTaq Plus Green 2X Master Mix (Lucigen), 0.5 µL of 20 µM of forward and reverse primers ...
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bioRxiv - Genomics 2021Quote: ... Prior to incubation with Terminator™ 5′-Phosphate-Dependent Exonuclease (TEX) (Lucigen) to remove all residual RNAs containing 5’ monophosphate ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted directly from the sorted nuclei without centrifugation using the MasterPure DNA Extraction kit (Epicentre, Madison, Wisconsin, USA) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification Kit (Epicentre, UK). During the extraction protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted (using MasterPure Yeast DNA Purification Kit; Epicentre, UK) from stationary phase and re-growth cultures for selected timepoints (Days 0 ...