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Citations for Lucigen :
1651 - 1700 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Ligase I (Lucigen) at 68°C for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... or Buffer J (EpiCentre) with Taq Polymerase (Roche).
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bioRxiv - Molecular Biology 2021Quote: ... For the subsequent PCR the Econo-Taq (Lucigen) master mix was used with forward (5’-TGACTATCCTAGAAATCGCTGTCG ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of Thermostable RNAseH (Epicentre) and 8 µl of RNAseH buffer (pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in 20 µL circularization reaction mix (7.75 mM Tris pH 8, 1x Epicentre CircLigase buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNase R (Lucigen) digestion was performed to degrade linear RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... and was circularized with Circligase (Lucigen), according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: Synthetic RNA substrate H4 (34) was radiolabeled at its 5’ end using T4 polynucleotide kinase (Epicentre) and [γ-32P]ATP (Perkin Elmer ...
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bioRxiv - Microbiology 2021Quote: ... coli C41(DE3) (Lucigen) with pEC86* ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the remainder of samples were lysed by adding 1 ul of ReadyLyse (EpiCentre) and incubating at room temperature for 12 hours ...
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bioRxiv - Microbiology 2021Quote: ... individual extracts were plucked off using sterilized forceps and placed into individual wells of a microplate containing 50 μl of QuickExtract buffer (EpiCentre). Extracts were disturbed by pipetting up and down (samples did not completely dissolve even after mixing) ...
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bioRxiv - Microbiology 2021Quote: ... flhD or complexed AsflhD with flhD was performed at 37°C in TMN buffer containing 1 µg tRNA for 15 min with RNase III (Epicentre). Samples were loaded on denaturing polyacrylamide gels together with an RNA alkaline ladder as in (75).
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from co-cultures with the MasterPure complete DNA purification kit (Lucigen). The proportion of G ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to prepare directional multiplex libraries ...
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bioRxiv - Bioengineering 2021Quote: ... The gRNA-msDNA hybrid was extracted with the QuickExtract RNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Founder mice were genotyped using FailSafe PCR Kit (EpiCentre) and run on a 12% polyacrylamide gel for heteroduplex assay ...
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bioRxiv - Genetics 2021Quote: ... DNA was end repaired by using End-It- DNA End-Repair Kit (Epicentre) and linker ligated with T4 ligase (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... All linearized DNA were removed with 0.4 units Plasmid-Safe™ DNase (Lucigen, E3110K) followed by a 1X SPRI beads purification.
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bioRxiv - Microbiology 2021Quote: Approximately 50 microliters of the thawed Omnigene preserved stool sample was added to a microfuge tube containing 350 μL Tissue and Cell lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products) ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 microliters of the thawed Omnigene preserved stool sample was added to a microfuge tube containing 350 μL Tissue and Cell lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products).
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bioRxiv - Microbiology 2021Quote: ... culture media (Lab M) and the other one into a microfuge tube containing 350 μL Tissue and Cell Lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products ...
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bioRxiv - Immunology 2021Quote: Mice facial and back skin were swabbed at the end of the experiment using a sterile foam tipped applicator (Puritan) that was moistened with yeast cell lysis buffer (Lucigen) and collected into sterile 2 ml tubes (Eppendorf) ...
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bioRxiv - Immunology 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Microbiology 2021Quote: ... and the phage pellet used for phage DNA preparation with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... Phage and bacterial genomic DNA were prepared with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Genomics 2021Quote: ... 2 uL of library ligation was added to 50 uL Endura cells (Lucigen) then electroporated ...
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bioRxiv - Genomics 2021Quote: ... 10uL 10x OmniAmp buffer (Lucigen, F883707-1), 8uL dNTP (NEB ...
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bioRxiv - Genomics 2021Quote: ... 10uL Betaine (Lucigen, F881901-1), 5uL random primer mix (NEB ...
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bioRxiv - Genomics 2021Quote: ... 6uL 100mM MgSO4 (Lucigen, F98695-1), 10uL Betaine (Lucigen ...
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bioRxiv - Genomics 2021Quote: ... 100μg total RNA from cortex or cultured HEK293 cells was treated with or without 1μL of RNaseR [20 U/μl] (Lucigen), plus 1.9μL RNaseOUT [40 U/μL] (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... genomic DNA was isolated from each sample using DNA QuickExtract (Lucigen, Cat #QE09050) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and DNA extraction was carried out with MasterPure Complete DNA Isolation kit (Lucigen) as indicated by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... The bacterial transformation was done in 50 reactions using 2.5 ul of purified eluate and 20 ul of Transformax EC100D pir-116 (Lucigen) by electroporation (Gene Pulser Xcell ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Genomics 2021Quote: ... 1 ml of recovery media (Lucigen) was added ...
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bioRxiv - Genomics 2021Quote: ... or Transformax EC100D pir-116 (Lucigen) electrocompetent cells for the pGL4.10-Sasaki-SS (a ...
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bioRxiv - Genomics 2021Quote: ... cloni 10G SUPREME (Lucigen) or Transformax EC100D pir-116 (Lucigen ...
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bioRxiv - Genomics 2021Quote: ... followed by dephosphorylation using T4 Polynucleotide Kinase (Epicentre) and final purification using ...
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bioRxiv - Genomics 2021Quote: ... 1.5 μl ATP and 1 μl FastLink DNA ligase (Lucigen Corporation), followed by heat inactivation for 15 min at 70°C ...
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bioRxiv - Genomics 2021Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen) (2 μl of the ligation mix per reaction ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA from each cell line was isolated (QuickExtract DNA Extraction Solution, Lucigen). Total RNA was isolated from each cell line (Maxwell RSC simplyRNA kit ...
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bioRxiv - Genomics 2021Quote: DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit and sequencing was performed on the Illumina NextSeq500 with 75bp single-end reads (first experiment) ...
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bioRxiv - Genomics 2021Quote: ... and the Globin-Zero Gold kit (Epicentre) to remove globin mRNA and ribosomal RNA ...
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bioRxiv - Immunology 2021Quote: ... coli C41 (Lucigen) and plated on LB-agar supplemented with 50μg/mL kanamycin and 1% glucose ...
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bioRxiv - Evolutionary Biology 2021Quote: Then the PCR products were ligated into vector pSmart LC Kn (Lucigen, cat.# 40821) and electroporated into E ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was amplified from MGH21 and ligated into pSmart LC Kn (Lucigen, cat.# 40821), generating pCas3CRISPR2 ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells (Strand et al., 2014; Lucigen, Middleton, WI, Catalog #EC300110) were transformed via heat shock with sequence-verified pPtPBR1 episomes ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from whole adult worms using the MasterPure™ DNA Purification kit (Epicentre Biotechnologies) at the Department of Veterinary Disease Biology (VDB) ...