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Citations for Lucigen :
1601 - 1650 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was isolated (Quick Extract DNA extraction solution, Lucigen), amplified by PCR ...
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bioRxiv - Cell Biology 2021Quote: ... coli EXPRESS BL21(DE3) chemically competent cells (Lucigen). A dense overnight culture was diluted 1:100 into 15 mL LB (Miller ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet resuspended in 100μL QuickExtract solution (Lucigen #QE09050), transferred to PCR-tubes and incubated in a thermocycler for 15 minutes at 68°C ...
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre). RNA fragmentation ...
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bioRxiv - Microbiology 2021Quote: ... The genomic DNA was extracted using the Lucigen (Epicentre; Middleton, WI, USA) Masterpure Gram Positive DNA Purification Kit per manufacturer’s instruction with the following modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library prep was performed according to the manufacturer’s instructions for the NxSeq® AmpFREE Low DNA Library Kit from Lucigen® (Cat No ...
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bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from mice faecal pellets obtained prior to RVwa inoculation from all experimental groups with the Master Pure™ kit (Epicentre), including a bead beater treatment with 0.5 g of 0.1 mm glass beads in the lysis step (FastPrep 24-5G Homogenizer ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of purified RNA was used in cDNA synthesis with MMLV HP Reverse Transcriptase (Lucigen RT80125K) and 100 ng random hexamers (IDT) ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments for blunt cloning were repaired using an End-It DNA End-Repair Kit (Lucigen, cat#ER0720) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and blunt ended ligation (Lucigen, cat#LK0750H) following manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Microbiology 2021Quote: ... The vector product was sized and purified from an agarose gel and circularized by end repair/phosphorylation (Lucigen, cat#ER0720) and blunt ended ligation (Lucigen ...
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bioRxiv - Microbiology 2021Quote: ... eDNA was purified from the samples by removing the proteins and RNA using MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), and the DNA concentration was measured using NanoVue Plus (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Fosmid libraries of peat lake and freshwater samples were constructed with a pCC2Fos copy control library kit according to manufacturer’s protocol (Epicentre Biotechnologies, Cat. No. CCFOS059), and 198 96-well plates (66 from each sampling location ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... This sequence was cloned into the pSmart (Lucigen Cat#40041-2) shuttle vector using Gibson assembly (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... 500 ng of total RNA were treated with DNAse (Baseline Zero DNAse, Epicentre) prior to ribosomal RNA depletion using the RiboZero bacteria magnetic Kit from Illumina according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... Genomic DNA was extracted with QuickExtract (Lucigen) and PCR was performed using Q5® High-Fidelity DNA Polymerase according to the manufacturer’s protocol (Forward primer ...
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bioRxiv - Neuroscience 2021Quote: ... The purified PCR product was inserted into digested backbone using Gibson assembly67 followed by ethanol precipitation and transformation of electrocompetent Endura cells (Lucigen). Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplicons were assembled into a full-length clone in a single-copy vector pCC1BAC (Epicentre) by using the NEBuilder HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2021Quote: NeuroD1-pNOTAT was transformed into C41 competent cells (Lucigen, Middleton, WI) and induced with IPTG at 0.1mM for protein expression ...
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bioRxiv - Neuroscience 2021Quote: ... the genomic DNA was extracted using the QuickExtract DNA Extraction solution (Epicentre, Madison, USA), according to the manufactureŕs instructions ...
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bioRxiv - Genomics 2021Quote: ... these BAC DNAs were completely digested with ATP-Dependent DNase (Epicentre) to remove the host E ...
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bioRxiv - Neuroscience 2021Quote: ProPeL experiments were carried out as previously described8 with the following conditions for in vivo proteome phosphorylation: all CK2 constructs were expressed in Escherichia coli OverExpress C43(DE3) cells (Lucigen) by IPTG induction ...
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bioRxiv - Bioengineering 2021Quote: ... coli TG1 (Lucigen, Middleton, WI, Cat#60502-02) to construct two libraries with sizes of 1 × 107 and 2 × 107 independent transformants for Green and Red ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using gene specific primers and EconoTaq PCR Master Mix (Lucigen) per the manufacturer’s guidelines (18 cycles for S14 and 24 cycles for U6) ...
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bioRxiv - Microbiology 2021Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Epicentre), 2 U/mL Zymolyase (Zymo research) ...
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bioRxiv - Microbiology 2021Quote: ... and subcloned into the NotI sites of pSMART BAC vector (Lucigen, Middelton, WI, USA). The luciferase reporter vector pGL4 was used as the template for PCR amplification of Renilla luciferase gene ...
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bioRxiv - Microbiology 2021Quote: ... and transformed into the BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen). The replicon vector was maxipreped using a NucleoBond Xtra BAC kit (Takara Bio.).
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bioRxiv - Bioengineering 2021Quote: ... Each library was independently electroporated into Endura electrocompetent cells (Lucigen), plated on agar bioassay plates ...
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bioRxiv - Bioengineering 2021Quote: ... PCR fragments were then in vitro transcribed using the AmpliScribe T7-Flash kit (Lucigen) with overnight incubation at 37°C ...
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bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
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bioRxiv - Microbiology 2021Quote: ... 4200 units of Ready-Lyse lysozyme (Lucigen), 30 units of DNase I (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... and mRNA was enriched using the Bacterial Ribo-Zero rRNA removal kit (Epicentre). Residual mRNA was converted to sequencing libraries using TruSeq barcoded adaptors (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... coli strain DH5α was isolated using the QuickExtract DNA extraction solution (Lucigen). The coding sequences of the four autoinducer-2 exporter genes were amplified using the Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Collagenase and EDTA solutions were prepared with RNAse inhibitor added (1:100 ratio, Lucigen, 30281). Isolation buffer is composed of 70 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was circularized by CircLigase™ II ssDNA Ligase (Epicentre, CL9025K). Next ...
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bioRxiv - Cell Biology 2021Quote: Total RNAs was treated with RNase R for 30 min at 37°C using 3 U/mg of RNase R (Lucigen, USA). HCC-LM3 and Huh-7 cells treated with actinomycin D (1 µg/mL ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 Star (DE3) or OverExpress C43 (DE3) (Lucigen/VWR, Radnor, PA). Following induction with IPTG ...
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bioRxiv - Microbiology 2021Quote: ... DNA from the cell pellets were extracted using a MasterPure™ DNA Extraction kit (Epicentre®, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 50U T7 RNA Polymerase (Lucigen), 1 μL RNase Inhibitor and 50~100 ng DNA template ...
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bioRxiv - Developmental Biology 2021Quote: ... then circularised using Circligase II (Epicentre) followed by AMPure XP beads purification ...