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Citations for Lucigen :
1251 - 1300 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was size selected and circularized with Circligase II (Epicentre). Circularized cDNA was cut with BamHI and amplified using Accuprime Supermix I (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 μl of Lysozyme (50 U/μl, Epicentre) and 1.95 μl of nuclease-free water (Ambion)) ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from whole blood following the MasterPure™ DNA Purification kit (Epicentre, MCD 85201). DNA passing quality control based on NanoDrop spectrometry and in sufficient amount through the Qubit dsDNA Broad Range Assay Kit (ThermoFischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions (Lucigen, Middleton, WI). The DNA was amplified with TMEM173 primers (key resource table) ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was extracted using GELase treatment (Epicentre Biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... was performed in a two-step barcoded PCR protocol using the FailSafe PCR PreMix (Lucigen, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: cDNA was made from 200 ng of labellar RNA as template from using EpiScript (Lucigen). Two biological replicates were prepared per species ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was harvested with QuickExtract (Lucigen) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.1% NxGen RNAse inhibitor (Lucigen; 30281-2)) ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 8-well strips containing 3 μL of cell collection buffer (0.1% Triton X-100, 0.2 U/μL RNAse inhibitor (Lucigen)) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 μL 40 U/μL RNasin (Lucigen), and 0.6 μL nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and NxGen RNase inhibitor (0.2U/uL; Lucigen). NAc tissues were dissected rapidly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... were ligated to the ChIP DNA (Fast-Link kit; Epicentre) and PCR amplified using the Illumina TruSeq ChIP Library Prep Kit ...
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bioRxiv - Neuroscience 2022Quote: ... coli (Lucigen 60242-2) using program EC1 on MicroPulser Electroporator (Bio-Rad 1652100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2000 K U/µl Ready-Lyse lysozyme (Epicentre)) and incubated at 37°C for 30 min to degrade the cell wall ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre) and sequencing was performed on NextSeq500 (single-end 96 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented gDNA was ended-repaired in 50 μl reaction using the End-It DNA End-Repair Kit (Lucigen, ER81050), incubated at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 2000 K U/µl Ready-Lyse lysozyme (Epicentre)) and incubated at 37°C for 30 min to degrade the cell wall ...
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bioRxiv - Microbiology 2022Quote: ... 5 U Baseline-ZERO DNase (Epicentre), 25 U Benzonase (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μg of RNA was treated with a beta version of Ribo-Zero Magnetic Gold Kit Yeast (Epicentre) to deplete rRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 1 μg purified RNA was applied to RT-qPCR and the remaining was subjected to ribosomal RNA depletion using a Ribo-Zero Gold Kit (Epicentre). Sequencing libraries were constructed from 100 ng total RNA using a Truseq stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
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bioRxiv - Microbiology 2022Quote: ... and the target exons were amplified with EconoTaq polymerase (Lucigen, Middleton, WI, USA). The resulting amplicons were subcloned using the TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... a PlasmidSafe DNase (PSD; Epicentre) experiment was performed as described previously [5] ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted using QE buffer (Lucigen) and PCR was performed using Q5 polymerase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Molecular Biology 2022Quote: Ribosomal RNAs (rRNA) were removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Madison, WI). For the whole biopsies ...
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bioRxiv - Microbiology 2022Quote: ... depleted in ribosomal RNA (Ribo-Zero rRNA Removal Kit, Epicentre) and reverse transcribed into cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Cell Biology 2022Quote: ... genomic DNA was extracted using the QuickExtract DNA extraction kit (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... TypeOne™ Restriction Inhibitor (Lucigen), EZ-Tn5
transposon (at 0.1 pmol/μL ... -
bioRxiv - Genomics 2022Quote: ... 1 U/μL RNase Inhibitor (NxGen, Lucigen), 1 U/μL reverse transcriptase (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted with the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, Cat. No.: MC85200). The Qubit 3.0 fluorometer and Nano-300 (Allsheng™ ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
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bioRxiv - Molecular Biology 2022Quote: ... For RNAse-free IP conditions TBS-0.05% NP-40 was supplemented with 1U/ul NxGen RNAse inhibitor (Lucigen). For nuclease treated samples ...
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bioRxiv - Genomics 2022Quote: ... Coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).