Labshake search
Citations for Lucigen :
1151 - 1200 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... RNase H (10 U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 μl containing 50 mM Tris-HCl (pH 7.4) ...
-
bioRxiv - Developmental Biology 2022Quote: ... End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25°C for 45 min ...
-
bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
-
bioRxiv - Cell Biology 2022Quote: ... the individual clones were expanded and the DNA was isolated using the QuickExtract DNA Extraction Solution (Lucigen). A successful homozygous genomic modification was confirmed by PCR (data not shown ...
-
bioRxiv - Cell Biology 2022Quote: ... genomic DNA was isolated with QuickExtract™ DNA Extraction Solution (Lucigen) and targeted sequences were amplified by PCR ...
-
bioRxiv - Genomics 2022Quote: DNA was isolated from the 16 parental strains using the Masterpure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). The Pacific Biosciences protocol “Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” was used to create libraries from 30 micrograms of DNA ...
-
bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
-
bioRxiv - Genetics 2022Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
-
bioRxiv - Genetics 2022Quote: ... Supernatants were discarded and the remaining 0.2 mL were digested with DNases (Promega and Epicentre, USA), RNase A (Ambion ...
-
bioRxiv - Genomics 2022Quote: ... Purified ChIP DNA was repaired with End-It DNA End-Repair Kit (Epicentre). The library was then indexed and amplified and sequenced on an Illumina platform ...
-
bioRxiv - Genomics 2022Quote: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction ...
-
bioRxiv - Genomics 2022Quote: ... from at least two freshly starved 9 cm plates of the appropriate worm strain using the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), following manufacturer protocols ...
-
bioRxiv - Genomics 2022Quote: ... and split into 6-well culture for expansion and into lysis buffer for DNA extraction (homemade by GESC, formulation identical to Lucigen Quick-Extract buffer). PCRs were performed with Platinum Superfi II 2x master mix (Thermofisher ...
-
bioRxiv - Genomics 2022Quote: ... DNA was purified from these cultures using the MasterPure™ Yeast DNA Purification Kit (Epicenter/Lucigen). Purified DNA was used to amplify each TLO gene using a centromeric chromosome arm specific primer (ALO36-48 and ALO60 ...
-
bioRxiv - Genomics 2022Quote: ... we treated the RNA with 10X Baseline-ZERO DNase (Lucigen, DB0715K). DNase I treated RNA samples were purified and further concentrated using RNA clean and concentrator-5 (Zymo Research ...
-
bioRxiv - Genetics 2022Quote: Genomic DNA was extracted with the MasterPure Yeast DNA Purification Kit (Lucigen) and DNA concentrations were measured using the Qubit Flex fluorometer and 1x HS dsDNA assay kit (Invitrogen) ...
-
bioRxiv - Genetics 2022Quote: ... and NxGen T7 RNA polymerase (Lucigen) at 37 °C for 18 hours ...
-
bioRxiv - Genetics 2022Quote: ... RNase Inhibitor (Lucigen) and NxGen T7 RNA polymerase (Lucigen ...
-
bioRxiv - Genetics 2022Quote: ... Genomic DNA was isolated from sporulation cultures using MasterPure Yeast DNA Purification kit (Lucigen MPY80200). Processing of genomic DNA samples and Southern blot analysis used to visualize crossovers and noncrossovers at the HIS4LEU2 hotspot were done as in [165] ...
-
bioRxiv - Genomics 2022Quote: ... it is possible to purchase the enzyme commercially (Lucigen TNP92110) and proceed directly to adapter annealing ...
-
bioRxiv - Genomics 2022Quote: ... Genomic DNA from edited and unedited HSPC samples was harvested 48h post-editing using QuickExtract DNA Extraction Solution according to manufacturer’s recommendations (Lucigen Corp., Teddington, UK) and diluted to 4.55 ng/uL in IDTE pH 8.0 (IDT ...
-
bioRxiv - Immunology 2022Quote: ... rinsed once with 1x phi29 polymerase buffer (Lucigen, NxGen kit 30221) in nuclease-free water ...
-
bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and genomic DNA was extracted from them using the MasterPure DNA Purification kit (Epicentre, Madison, WI, USA). Genomic DNA from one additional human iPSC (AG25370 ...
-
bioRxiv - Genetics 2022Quote: ... 20uL of Quick Extract buffer (Lucigen QE0905T) was added ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Purified genomic DNA from each individual was used to prepare paired-end libraries (Lucigen Shotgun library preparation, PCR free) and sequenced on an Illumina HiSeq X PE 150 bp platform to 10-20X coverage at the Centre d’ Expertise et de Services ...
-
bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
-
bioRxiv - Genetics 2022Quote: ... RNA isolation was performed according to the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... These solutions (5.5 µl each) were then used as template for a 20 µl reaction with the AmpliScribeTM T7 Transcription Kit (Lucigen, AS3107). The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch ...
-
bioRxiv - Immunology 2022Quote: ... was amplified using Endura competent cells (Lucigen) according to instructions provided by the Zhang lab and Lucigen as previously described (Dufva et al. ...
-
bioRxiv - Genomics 2022Quote: ... respectively) had their total genomic DNA extracted using MasterPure Yeast DNA Purification Kit (Lucigen), with cell lysis solutions and RNAse A treatment volumes scaled up 4x to adequately lyse all cells and remove RNA ...
-
bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
-
bioRxiv - Genomics 2022Quote: ... the ScriptSeq™ Index PCR Primers (Sets 1 to 4) and the FailSafe™ PCR enzyme system (all sourced from Epicentre®/Illumina® Inc., Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: Total RNA was extracted from 50mL flash-frozen cell pellets grown to mid-log (OD600 ∼0.65-0.85) using MasterPure™ yeast RNA purification kit (Lucigen) including a DNaseI treatment step ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 μL of each required DNA fragment and corresponding bridging oligo were added to 2.5 μL of Ampligase 10X Reaction Buffer (Lucigen, USA), 2.25 μL of 5 M betaine ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The Ampligase Thermostable DNA Ligase for LCR reaction was obtained from Lucigen (Middleton, USA).
-
bioRxiv - Synthetic Biology 2022Quote: ... and 1.5 μL of 5 U μL-1 Ampligase Thermostable DNA Ligase (Lucigen, USA). Water was added to a total volume of 25 μL ...
-
bioRxiv - Plant Biology 2022Quote: ... The DNA library was prepared using a NxSeq AmpFREE Low DNA Library kit (Lucigen) and sequenced on a Illumina HiSeq2000 with a 100bp paired-end mode ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli (Lucigen EC300150), recovered in LB + 5 mM MgCl2 at 30°C for 1 hour and then selected on LB + kanamycin ...
-
bioRxiv - Microbiology 2022Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Lucigen), 2 U/mL Zymolyase (Zymo Research Corporation) ...
-
bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... DNA extraction from pellets was done using MasterPure™ DNA purification kit (Epicentre, US) according to the manufacturers protocol ...
-
bioRxiv - Microbiology 2022Quote: ... difficile colonies on TCCFA were transferred to 25 µL of PCR-Lyse™ (Epicentre, Madison, WI, USA) solution ...
-
bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
-
bioRxiv - Physiology 2022Quote: ... sorted (eGFP+) cells was extracted using QuickExtract DNA Extraction Solution (Lucigen). EnGen Mutation Detection Kit (New England BioLabs ...
-
bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... and electroporated with the Ez-Tn5
transposome (Epicentre, Charlotte, North Carolina). Cells recovered at 30°C shaking for 90 minutes ... -
bioRxiv - Microbiology 2022Quote: ... 16S and 5S rRNA using the RiboZero rRNA Removal Kit (Epicentre) (two times) ...