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Citations for Lucigen :
1301 - 1350 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Linear single stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
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bioRxiv - Microbiology 2022Quote: ... and 10,000 units of ReadyLyse Lysozyme solution (Lucigen). Samples were subjected to bead beating for ten minutes at maximum speed on a vortex mixer with 0.5 mm glass beads (MoBio) ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Microbiology 2022Quote: ... 0.025 µL NxGen RNase inhibitor (Lucigen, CN 30281), 8 µL nuclease-free H2O) ...
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bioRxiv - Microbiology 2022Quote: ... sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre), DNase treated ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Immunology 2022Quote: ... and genomic DNA isolated using QuickExtract (Lucigen). The gRNA binding sites were amplified using KOD Hot Start Polymerase (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and inserted into pJAZZ-OK using BigEasy® v2.0 Linear Cloning Kit (Lucigen) to increase plasmid copy number (Figure S3b) ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of genomic DNA used the MasterPure™ Yeast DNA Purification Kit (Lucigen # MPY80200) with a NextSeq500– 1flowcell mid output (130M fragments) ...
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bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed on a Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... Escherichia coli (Epi300, Lucigen) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2022Quote: ... Escherichia coli (Epi300, Lucigen) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Plant Biology 2022Quote: ... Contaminating DNA was removed from total RNA samples with Baseline-ZERO DNase (Epicentre), whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre) ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific libraries were prepared from ribosomal RNA-depleted total RNA using ScriptSeq complete kit for plant leaf (Epicentre; BPL1224) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre). Libraries were sequenced for 2 × 100-bp reads on an Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2022Quote: ... whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre). Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre) ...
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bioRxiv - Systems Biology 2022Quote: ... treated with Baseline-ZERO DNase (Epicentre), and cleaned again by RNeasy Mini ...
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bioRxiv - Plant Biology 2022Quote: ... The RNAs then were subjected to RNase R (Part number: E0111-20D1, Lucigen) treatment following the manufacturer’s instruction in a 20 µl reaction in the presence of lithium chloride containing buffer (Xiao and Wilusz ...
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bioRxiv - Neuroscience 2022Quote: 30 µl of Quick Extract DNA Estraction (Lucigen, QE09050,) was added to 5.0 x 104 pelleted iPSC ...
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bioRxiv - Neuroscience 2022Quote: RNAse R (Lucigen, #RNR07250) treatment was performed on 1 μg total RNA for 10 minutes at 37°C using 5U of enzyme ...
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bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was size selected and circularized with Circligase II (Epicentre). Circularized cDNA was cut with BamHI and amplified using Accuprime Supermix I (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 μl of Lysozyme (50 U/μl, Epicentre) and 1.95 μl of nuclease-free water (Ambion)) ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from whole blood following the MasterPure™ DNA Purification kit (Epicentre, MCD 85201). DNA passing quality control based on NanoDrop spectrometry and in sufficient amount through the Qubit dsDNA Broad Range Assay Kit (ThermoFischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions (Lucigen, Middleton, WI). The DNA was amplified with TMEM173 primers (key resource table) ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was extracted using GELase treatment (Epicentre Biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... was performed in a two-step barcoded PCR protocol using the FailSafe PCR PreMix (Lucigen, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) cells (Lucigen, Middleton, WI) freshly transformed with the Gαiq expression plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) cells (Lucigen, Middleton, WI) freshly transformed with the βArr1-3A expression plasmid ...
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bioRxiv - Bioengineering 2022Quote: ... genomic DNA was extracted 72 hrs after transfection using QuickExtract (Epicentre). For flow cytometry assays ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated genomic DNA was combined with EconoTaq PLUS GREEN 2X Master Mix (cat# 30033-1; Lucigen, Middleton, WI, USA) and forward and reverse primers (primer information listed in Table S2 ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was carried out with EconoTaq PLUS GREEN 2X Master Mix (cat# 30033-1; Lucigen) and an initial denaturation at 94°C for 2 min ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted using the MasterPure Yeast DNA Purification Kit (Epicentre). An Illumina sequencing library was prepared using home-made Tn5 transposase as previously described (Tao et al ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NxSeq AmpFREE Low DNA Fragment Library Kits (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... rRNA was removed using a Ribo-Zero kit (Epicentre, Madison, WI) and mRNA was randomly fragmented ...
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bioRxiv - Genetics 2022Quote: ... single BFU-E and CFU-GM were lysed with QuickExtract Lysis Buffer (Epicentre). CFCs were incubated at 65°C for 20 min followed by an incubation at 98°C for 10 min and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from cell pellets using the MasterPure™ Yeast DNA Purification Kit (Lucigen, cat #MPY80200), with an additional initial incubation with zymolyase at 37°C to enhance cell lysis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The Tn7 integration plasmids with barcode and spectinomycin cassette were extracted from TransforMax EC100D pir + cells (Lucigen) with a Qiagen midi kit ...
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bioRxiv - Genetics 2022Quote: ... The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen) using Gene Pulser Xcell Electroporation Systems (Bio-Rad) ...