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Citations for Lucigen :
1401 - 1450 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... dsRNA was synthesized from in vitro transcription reactions (NxGen, Lucigen). dsRNA corresponding to Caenorhabditis elegans unc-22 ...
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bioRxiv - Developmental Biology 2021Quote: Riboprobes and double-stranded RNA (dsRNA) for src-1 were generated by in vitro transcription (NxGen, Lucigen) as described previously (Petersen and Reddien ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 10 U/uL Rec J exonuclease (Epicentre RJ411250), and the included buffer were added and incubated at 30 C for 45 minutes.
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bioRxiv - Evolutionary Biology 2021Quote: ... dsRNA was synthesized from the purified DNA using Ampliscribe T7-Flash Transcription kits (Epicentre Technologies, Co., Wisconsin, USA). We designed the PCR primers using the Primer3Web version 4.1.0 (Untergasser et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a (modified) CTAB protocol or a rapid desalting method (MasterPure™ Complete DNA and RNA Purification Kit; Lucigen Corporation).
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bioRxiv - Neuroscience 2021Quote: The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
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bioRxiv - Neuroscience 2021Quote: ... 6µl 500X CopyControl Fosmid Autoinduction Solution was also added to induce the fosmid to high copy according to the CopyControl Fosmid Library Production Kits (EPICENTRE). The fosmid was then prepared using the standard Qiagen miniprep kit.
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bioRxiv - Neuroscience 2021Quote: ... in the pET-28 bacterial expression vector together with pLysSRARE2 plasmid (both kindly shared by Dr. Chernomordik, NICHD) were electroporated into ClearColi™ BL21(DE3) according to manufacturer protocol (Lucigen, WI). Bacterial colonies that grew in an LB plate containing 100 μg/ml kanamycin and 30 μg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNAs were circularized by CircLigaseII (Lucigen) and PCR-amplified ...
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bioRxiv - Molecular Biology 2021Quote: ... The selected cDNAs were subsequently circularized using CircLigase (CL4111K, Epicentre) and relinearized with ApeI (M0282S ...
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bioRxiv - Molecular Biology 2021Quote: ... We first repaired the purified DNA again using the End-it DNA End-Repair Kit (Lucigen #ER0720) following the manufacturer’s suggested conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Two micrograms of starting material were treated with Baseline-ZERO™ DNase (Lucigen, USA) and ribosomal RNA was removed using the Ribo-Zero™ Magnetic Core Kit and Ribo-Zero™ rRNA Removal kit (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted after 48-72 hrs incubation using 50 µL Quick Extract™ DNA Extraction Solution (Lucigen, Middleton, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were produced using the Art-Seq kit (Epicentre/Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed using Ribo Zero Gold (Epicentre/Illumina; 20020596) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then thawed and 35,000 U of Ready-Lyse (Epicentre) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Molecular Biology 2021Quote: ... after which genomic DNA was isolated using QuickExtractTM DNA Extraction Solution (Epicentre, Madison, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 µl Hybridase (Lucigen, H39500, 5 units/µl) and 2.5 µl water were added to the mix ...
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bioRxiv - Molecular Biology 2021Quote: ... coli RNA polymerase σ70 holoenzyme (Epicentre) or S ...
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bioRxiv - Molecular Biology 2021Quote: ... and 0.1 U/μL ReadyLyse Lysozyme (Lucigen). The suspension was incubated for 5 min at RT and then for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with Terminator (Lucigen) following the manufacturer’s protocol using the supplied Buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 U/μL ReadyLyse lysozyme (Epicentre) and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA of edited cells were extracted using Quick Extraction kit (Lucigen). Target sites carrying gene-edited sequences were PCR amplified using gene-specific primers (Additional file 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The ligation product was purified by isopropanol precipitation and then transformed into electrocompetent Escherichia coli (Lucigen, Middleton, WI, USA). Transformed cells were plated on to 15 cm Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2021Quote: ... circularized by CircLigase™ II ssDNA ligase (Lucigen). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... The ssDNA linker containing a 5′ phosphate and 3′ C3 spacer was ligated to the synthesized cDNA using 20 U of the Circligase I (Lucigen, Middleton, WI). The resultant cDNA was amplified by an adapter-based PCR using the KAPA HiFi DNA polymerase (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The eluted vRNA was treated with 10 U of RNaseR (Lucigen) in an RNaseR buffer (20 mM Tris- HCl [pH 8.0] ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and the libraries have been generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq was performed on RNA depleted of rRNA using a yeast Ribo-Zero kit (Epicentre). Samples were multiplexed and sequenced on a HiSeq analyzer (Illumina).
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bioRxiv - Molecular Biology 2021Quote: ... RNA was extracted using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB M0368X ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... using Endura™ ElectroCompetent Cells (Lucigen 60242-1). Resulting pooled DNA libraries were used to produce lentiviral particles and infect primary cortical neurons.
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bioRxiv - Biochemistry 2021Quote: ... coli Cloni® 10G cells (Lucigen, USA). Each amplicon was mixed with cleaved plasmid in the ratio ...
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bioRxiv - Biochemistry 2021Quote: ... coli Cloni® 10G cells (Lucigen, USA). The clones were screened by colony PCR and their correctness was verified by sequencing.
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bioRxiv - Genomics 2021Quote: ... MDA reactions were run under clean conditions using the RepliPhi kit (Epicentre) in a 15 ul reaction volume in 96-well plates or with the Repli-g Single Cell kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... DNA extraction from 2 x 2 x 2mm fin-tissue clips was performed in a 96-well PCR plate using 100μl of QuickExtract solution (Lucigen, USA) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
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bioRxiv - Genetics 2021Quote: Endura electrocompetent cells (Lucigen) were electroporated with the genome-scale CRISPR KO (Brunello ...
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bioRxiv - Genetics 2021Quote: ... The DNA:RNA hybrids were digested with 10 U Thermostable RNase H (Epicentre, H39500) in 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Genomics 2021Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by CIP (NEB ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using the Epicenter Masterpure kit (Epicentre Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... We performed 9 electroporations in total of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...