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Citations for Lucigen :
1051 - 1100 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... samples were treated with RNAseR (Epicentre, Teddington, UK), and reverse transcribed by using fluorescently labeled random primers ...
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bioRxiv - Cancer Biology 2022Quote: ... and CAGs-rtTA3 PCRs using EconoTaq PLUS (Lucigen #30033-2). Doxycycline chow (food pellets ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA of grown clones was harvested using QuickExtract™ DNA Extraction Solution (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3.2U PlasmidSafe Exonuclease (Epicentre, E3101K) and was incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... It was then purified using the MasterPure Gram Positive DNA purification kit (Lucigen, Middleton, Wisconsin, USA) using the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... DNA was isolated from overnight cultures using a MasterPure™ Gram Positive DNA Purification Kit (Epicentre, USA). Nanopore libraries were prepared using Ligation Sequencing Kit (SQK-RBK109 ...
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bioRxiv - Microbiology 2022Quote: ATP-Dependent DNase kit (Lucigen, catalog #: E3110K).
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bioRxiv - Microbiology 2022Quote: DNA was extracted from 154 clinical and environmental samples using the MasterPure™ DNA Purification Kit (Epicentre), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... 16 U of DNA Ampligase (Epicentre Technologies, Madison, WI), 0.016 mM dNTP ...
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bioRxiv - Genomics 2022Quote: ... 1x Ampligase DNA ligase buffer (Epicentre Technologies, Madison, WI), 0.5 μl of 5M Betain (Sigma Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... A total of 1.2 µg of ligated pUSEBR-CRISPR Library plasmid DNA was then electroporated into Endura electrocompetent cells (Lucigen). Competent cells were recovered for 1 h at 37ºC ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the Quantseq 3’ mRNA-Seq Library Prep Kit FWD (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was isolated from PHH using a MasterPure Complete DNA Purification kit (Epicentre, Madison, WI; MC85200) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The Gibson assembly reaction was electroporated using Endura(tm) ElectroCompetent Cells (Lucigen), plated on LB + Ampicillin plates at an estimated 150,000 colonies per replicate library to limit library complexity ...
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bioRxiv - Evolutionary Biology 2022Quote: ... end-repaired using End-It™ DNA End-Repair Kit (Lucigen) and A-tailed using Klenow Fragment (3’→5’ exo- ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Genomics 2022Quote: ... The product was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Genetics 2022Quote: ... 50ng ribo-depleted RNA was used for library preparation with the Scriptseq kit (Epicentre, cat. no. SSV21124) following the manufacturer’s protocol but with the following modifications ...
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bioRxiv - Genomics 2022Quote: ... and diluted at 1:100 ratio into 250 ml LB supplemented with kanamycin (50 μg/mL) and 1x copy number induction solution (Lucigen, CCIS125). Payload DNA was isolated from E ...
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bioRxiv - Genomics 2022Quote: Shot-gun library was generated at the McGill Genome Center using the NxSeq® AmpFREE Low DNA Library Kit Library Preparation Kit (Lucigen Corp., WI, USA) according to the manufacturer’s recommendations and the library was ran on a HiSeq X for 2×150 cycles.
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bioRxiv - Genetics 2022Quote: Transposon mutagenesis was performed using the EZ-Tn5
transposon kit (Epicentre) and EK54/MA56/LP372 Tn5 transposase ... -
bioRxiv - Cancer Biology 2022Quote: ... The custom gRNA library was amplified in electrocompetent Endura bacteria (Lucigen, Cat. No. 60242) and plasmid DNA purified as described ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells in each well were used to purify genomic DNA via the Epicentre MasterPure DNA Purification Kit (Epicentre). PCR was used to amplify the CRISPR reaction locus with designed primer pairs ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (6 μg) from HG003 Δfur was treated with TAP (Epicentre) for 1 h at 37°C ...
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bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were confirmed by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was extracted using QuickExtract (Lucigen) as previously described (21) ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA for genotyping was extracted using QuickExtract DNA Extraction Solution (Lucigen, QE09050) from harvested yolk sac tissue if available or else from a micro-dissected nick of the extraembryonic anterior proximal region ...
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bioRxiv - Immunology 2022Quote: ... gDNA was prepared from clonal lines using QuickExtract (Lucigen), and the XBP1 locus was amplified by PCR and sequenced by Sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... cloni 10G cells (Lucigen) by electroporation (1.8 kV) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA of the two parents and the F2 progeny was extracted with the QuickExtract DNA Extraction Solution (Lucigen, QE0905T) according to manufacturer’s protocol with modifications ...
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bioRxiv - Biochemistry 2022Quote: ... The electroporated bacteria were then cultured in recovery medium (Lucigen 80026-1) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were then in vitro transcribed with T7 polymerase (Lucigen). Capture SELEX was then carried out as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... DNase I (Epicentre) digestion was performed according to the manufacture’s instruction to minimize contamination with plasmid DNA ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Bioengineering 2022Quote: ... coli SS320 cells (Lucigen) containing the circular phagemid comprising the target scaffold sequence and a M13cp helper plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... The library was transformed into electrocompetent Lucigen Endura™ Escherichia coli (Lucigen; cat. 60242-2) using a Bio-Rad MicroPulser Electroporator (#1652100) ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... in combination with 1 μl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... shRNAs were synthesized by in vitro transcription (IVT) from double stranded DNA templates using the AmpliScribeTM T7-flashTM transcription kit (Lucigen, Inc.) and were purified using Direct-Zol TM RNA Miniprep kits (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: The RNAs from Mucor strains were extracted using the MasterPure™ yeast RNA purification kit (Lucigen MPY03100). Extracted RNA was then converted to cDNA by utilizing a high-capacity cDNA reverse transcription kit (Applied Biosystems™ 4374966) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmid-safe ATP-dependent DNase was obtained from Lucigen, WI ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of eluent was then electroporated into each of 2 tubes of 50 uL Endura electrocompetent cells (Lucigen, Cat#60242-2) as per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or the Epicentre MasterPure DNA Purification Kit (Epicentre Biotechnologies). All plasmid constructs were built with the Gibson Assembly Master Mix or the NEB Builder HiFi DNA Assembly (both New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and three libraries for each sex were generated with the NEBNext Ultra RNA library prep kit combined with rRNA depletion via the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies). Samples were sequenced on an Illumina HiSeq2500 platform at the Vienna BioCenter Core Facilities (VBCF ...