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Citations for Lucigen :
1201 - 1250 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Gel-purified cDNAs were then circularized with CircLigase II (Lucigen, Cat: CL4115K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The 3’end of resultant cDNAs were ligated to a ssDNA linker (5’-PhosNNNAGATCGGAAGAGCGTCGTGTAG-/3SpC3/3’) using Circligase ssDNA Ligase (Epicentre) at 65°C for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Molecular Biology 2022Quote: Approximately 1e+06 cells were harvested two days post nucleofection and incubated in 200 μL of QuickExtract DNA Extraction Solution (Lucigen) at 65ºC for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Microbiology 2022Quote: ... we redesigned the EZ-Tn5 Transposon Vector (Epicentre) so that the transposon insertion has ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting ligation mixture was electroporated into TG-1 phage-display competent cells (#60502-1, Lucigen) and plated onto 2xYT agar containing 100 µg/mL carbenicillin and 2% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... T7 promoter (TAATACGACTCACTATAG) followed by the first 1629 nucleotides of the VA1 genome inserted into the pSMART plasmid (Lucigen). A second plasmid ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the samples was isolated using MasterPure Complete DNA & RNA Purification Kit (Lucigen, Middleton, WI) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The library was electroporated into Endura electrocompetent cells (# 60242; Lucigen Bioresearch Technologies) and plasmid was prepared from > 80 million bacterial colonies (>1,000X representation) ...
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bioRxiv - Microbiology 2022Quote: ... theta was end-repaired using End-IT DNA End-Repair kit (Lucigen) in a 50 μL reaction consisting of 2 μL End-It Enz mix ...
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bioRxiv - Microbiology 2022Quote: ... coli EPI300 (Lucigen). An aliquot was taken out for CFU counting on LB-Kan50 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from flies using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) and converted to cDNA with the PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted using the Ribo-Zero™ Kit (Epicentre, Madison, WI, USA). The viral dsRNA of these strains was extracted and prepared as previously described [22] ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted using Lucigen Kit as per manufacturer’s instructions (Bioresearch, Lucigen, UK) and samples were sequenced by Novagene (Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... 2 ml/L fosmid autoinduction solution (Epicentre), each plate supplemented with 0.3% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... After the in vitro packaging into the phage lambda (MaxPlax™ Lambda Packaging Extract, Epicentre), the transfected phage T1-resistant EPI300™-T1R E ...
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bioRxiv - Microbiology 2022Quote: High molecular weight DNA extracted from all enrichment cultures was combined in equimolar amounts and used to prepare two metagenomic fosmid libraries ‘IS_Lib1’ (Cavascura enrichments) and ‘IS_Lib2’ (Maronti enrichments) using the CopyControl™ Fosmid Library pCC2FOS Production Kit (Epicentre Technologies, Madison, USA). DNA was end-repaired to generate blunt-ended 5’-phosphorylated fragments according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... (Amsterdam, The Netherlands) using standard pCC2FOS sequencing primers (Epicentre). Non-redundant fosmids were selected ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments in the range of 30-40 kbp were resolved by gel electrophoresis (2 V cm-1 overnight at 4 °C) and recovered from 1% low melting point agarose gel using GELase 50X buffer and GELase enzyme (Epicentre). Nucleic acid fragments were then ligated to the linearized CopyControl pCC2FOS vector following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 40 U of RiboGuard RNase Inhibitor (Lucigen), and 1 µM of the proteins of interest ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Microbiology 2022Quote: ... Linear single stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
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bioRxiv - Microbiology 2022Quote: ... and 10,000 units of ReadyLyse Lysozyme solution (Lucigen). Samples were subjected to bead beating for ten minutes at maximum speed on a vortex mixer with 0.5 mm glass beads (MoBio) ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Microbiology 2022Quote: ... 0.025 µL NxGen RNase inhibitor (Lucigen, CN 30281), 8 µL nuclease-free H2O) ...
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bioRxiv - Microbiology 2022Quote: ... sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre), DNase treated ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Immunology 2022Quote: ... and genomic DNA isolated using QuickExtract (Lucigen). The gRNA binding sites were amplified using KOD Hot Start Polymerase (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and inserted into pJAZZ-OK using BigEasy® v2.0 Linear Cloning Kit (Lucigen) to increase plasmid copy number (Figure S3b) ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of genomic DNA used the MasterPure™ Yeast DNA Purification Kit (Lucigen # MPY80200) with a NextSeq500– 1flowcell mid output (130M fragments) ...
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bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed on a Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... Escherichia coli (Epi300, Lucigen) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2022Quote: ... Escherichia coli (Epi300, Lucigen) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Plant Biology 2022Quote: ... Contaminating DNA was removed from total RNA samples with Baseline-ZERO DNase (Epicentre), whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre) ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific libraries were prepared from ribosomal RNA-depleted total RNA using ScriptSeq complete kit for plant leaf (Epicentre; BPL1224) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre). Libraries were sequenced for 2 × 100-bp reads on an Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2022Quote: ... whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre). Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre) ...
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bioRxiv - Systems Biology 2022Quote: ... treated with Baseline-ZERO DNase (Epicentre), and cleaned again by RNeasy Mini ...
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bioRxiv - Plant Biology 2022Quote: ... The RNAs then were subjected to RNase R (Part number: E0111-20D1, Lucigen) treatment following the manufacturer’s instruction in a 20 µl reaction in the presence of lithium chloride containing buffer (Xiao and Wilusz ...
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bioRxiv - Neuroscience 2022Quote: 30 µl of Quick Extract DNA Estraction (Lucigen, QE09050,) was added to 5.0 x 104 pelleted iPSC ...
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bioRxiv - Neuroscience 2022Quote: RNAse R (Lucigen, #RNR07250) treatment was performed on 1 μg total RNA for 10 minutes at 37°C using 5U of enzyme ...