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Citations for Lucigen :
1451 - 1500 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... cloni 10G electrocompetent cells (Lucigen) using the accompanying protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 18.75 U RNase R (Lucigen), 4.375 U RNase T (NEB) ...
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bioRxiv - Genetics 2021Quote: ... RNA samples for small RNA-seq were treated with 5’polyphosphatase (Lucigen) and prepared using the SMARTer Apollo system with modifications for small RNA library preparation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sample was treated with 20 units of 5’ RNA polyphosphatase (Lucigen) for 30 min at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... then further purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, USA). 100 ng of DNA was used for qPCR ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction for wild type and Sts5-2A cells was performed using MasterPure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... About 30 ug of total gDNA was recovered from using MasterPure™ Complete DNA and RNA Purification kit (Lucigen), and 0.5ug-1ug gDNA was checked on a 1% agarose gel for integrity and quality ...
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bioRxiv - Genomics 2021Quote: ... Following DNAse I digestion using the Baseline-ZERO Kit (Epicentre, Madison, Wisconsin), the RNA concentration was quantified using the Qubit RNA HS Assay Kit on a Qubit Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and additionally purified with a mRNA-ONLY Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The cloned library was expanded in Electrocompetent E.coli (Lucigen) and sequence complexity was analyzed using next generation sequencing using an Illumina MiSeq ...
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bioRxiv - Microbiology 2021Quote: ... coli C41(DE3) cells (Lucigen). Transformants were grown at 37°C and 225 rpm until an OD600 of 0.4–0.6 was reached ...
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bioRxiv - Microbiology 2021Quote: Purified cDNA was subject to circularization via CircLigase ssDNA Ligase (Lucigen) per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... DNA precipitations were completed with a MasterPure Gram Positive DNA Purification kit (Epicentre, Illumina ...
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bioRxiv - Microbiology 2021Quote: DNA isolation from inactivated cells was carried out using MasterPure™ Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was extracted using QuickExtract genomic DNA extraction solution (Epicentre Biotechnologies) and then screened by PCR amplification of the region of interest ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 100,000 cells per condition using DNA QuickExtract (Lucigen), and incubated at 65°C for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... The dsDNA library was then converted to modified-base RNA using the Durascribe T7 Transcription Kit (Lucigen, MA170E). This kit produces RNA that is resistant to RNase A degradation through the replacement of canonical CTP and UTP with 2’-fluorine-dCTP (2’-F-dCTP ...
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bioRxiv - Bioengineering 2021Quote: ... cloni DH10B (Lucigen) for achieving large library size ...
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bioRxiv - Bioengineering 2021Quote: ... coli electrocompetent cells (Lucigen) then plated on a 245 × 245 mm LB and kan agar plate ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was extracted (DNA extraction solution, Epicentre Biotechnologies) and edited regions were specifically amplified by PCR (primers are listed in Supplementary Table 1) ...
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bioRxiv - Biochemistry 2021Quote: 5’ RNA polyphosphatase (Rpp) and T4 Polynucleotide Kinase (PNK) were purchased from Epicentre and NEB ...
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bioRxiv - Biochemistry 2021Quote: ... genomic DNA was extracted using QuikExtract DNA reagent (Lucigen, Middleton, WI). Except where noted ...
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bioRxiv - Biochemistry 2021Quote: ... The dsDNA was purified from a 1% agarose gel and electroporated into E.coli SS320 (Lucigen) electrocompetent cells pre-infected with M13KO7 helper-phage (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... 47 U/μL Ready Lyse Lysozyme (Epicentre), and 1x PIC (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... the genomic DNA derived from transfected single cell colonies was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for Sanger sequencing and the genomic DNA with biallelic editing was further subjected to whole-genome sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... from which the genomic DNA was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for subsequent sequencing analysis.
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bioRxiv - Neuroscience 2021Quote: ... tail samples were lysed in QuickExtract DNA Extra Solution 2.0 (Lucigen, USA) and PCR reactions were carried out with GoTaq DNA polymerase and with the Primers ...
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bioRxiv - Neuroscience 2021Quote: ... and NxGen RNase Inhibitor (0.2 U/μl; Lucigen, 30281-2). Tissue was then triturated by fire-polished Pasteur pipette (three pipettes of decreasing diameter ...
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bioRxiv - Neuroscience 2021Quote: ... Insert-DNA (50 ng) was ligated with vector-DNA (150 ng) using the Fast-Link DNA Ligation Kit (LK0750H, Lucigen). XL10-Gold ultracompetent cells were transformed with the ligation product and streaked out on agar plates containing Kanamycin ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was purified using the MasterPure Gram-positive DNA purification kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μg total RNA was incubated with 4U/μg RNase R (#RNR07250, Lucigen) in 10μl reaction system at 37°C for 20min ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Cancer Biology 2021Quote: ... All the clones were expanded and DNA was isolated using the FosmidMax DNA Purification Kit (Epicentre). DNA from each clone was labeled through nick translation with either the Atto550 NT Labeling Kit (Jena Bioscience ...
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bioRxiv - Microbiology 2021Quote: The EZ-Tn5
Tnp transposome (Epicentre Biotechnologies, U.S.A) was introduced into E ... -
bioRxiv - Cell Biology 2021Quote: ... gDNA was extracted using QuickExtract™ (Lucigen). Cells were resuspended in 30 μl QuickExtract and incubated at 65°C for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed and genomic DNA was extracted using QuickExtract DNA Extraction Buffer (Lucigen) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid-Safe ATP-Dependent DNase was from Lucigen (Middleton, WI). Surveyor Mutation Detection Kit S100 was from Integrated DNA Technologies (Coralville ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng of each sample were PCR amplified using the FailSafe PCR system with PreMix H (Lucigen). Southern blot-based detection of telomere PCR products was modified from previous protocols 48,49 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen), 2 μl Maxima H- RTase (EP0751 ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl RNase Inhibitor (30281, Lucigen) and 31 μl water ...
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bioRxiv - Physiology 2021Quote: ... mRNA was purified from 100 ng of total RNA by using a Ribo-Zero rRNA removal kit (Epicentre) to deplete ribosomal RNA and convert into double-stranded complementary DNA by using an NEBNext mRNA Second Strand Synthesis Module (E6111L) ...
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bioRxiv - Plant Biology 2021Quote: ... which were indexed using ScriptSeq Index PCR Primers (RSBC10948, Epicentre). Sequencing was performed on an Illumina HiSeq instrument (Microgen ...
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bioRxiv - Plant Biology 2021Quote: ... A Ribo-Zero magnetic kit (MRZPL116, Epicentre) was used for rRNA depletion from total RNA ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of rRNA-depleted RNA was used to construct sequencing libraries using a ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre). Twelve PCR cycles were used for amplification of the libraries ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA (1-3 μg) was then treated with 5 units of RNase R (Lucigen. RNR07250) for one hour at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... a DNase digest was performed using Baseline-ZERO™ DNase (Epicentre, Madison, USA). 3.4 µL of the reaction buffer was added to 30 µL total RNA ...