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Citations for Lucigen :
1351 - 1400 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 2.3μL U/μL Ampligase (Lucigen A0102K), 1.5 μL Reducing agent B (10x Genomics 2000087) ...
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bioRxiv - Microbiology 2022Quote: ... 8μL of the oligo-glycerol sample was mixed with 2μL of EZ-Tn5 (Lucigen, TNP92110) and incubated at 25°C for 40 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... gDNA was isolated from obtained cell clones using QuickExtract (Lucigen), the gRNA-targeted sites were PCR-amplified ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Cell Biology 2022Quote: ... extraction of genomic DNA was performed with QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with DNase I (cat# D9905K; Lucigen, Middleton, WI, USA). The concentration and purity of the isolated RNA were determined using a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Escherichia coli (Epi300, Lucigen, Cat #: EC300110) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid pHCKan-yibDp-GFP was constructed as described previously.10 Plasmid pHCKan-yibDp-tsGFP was assembled from a gBlock™ and store-bought linearized pHCKan plasmid (Lucigen, Middleton, WI) using NEBuilder® HiFi DNA Assembly Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Biophysics 2021Quote: ... L and Neg sorted pools using 100 μL QuickExtract (Lucigen) per 1 million cells ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Lysate corresponding to 30 μg RNA was diluted to 200 μl in polysome buffer and digested with 1.5 μl RNaseI (Epicentre) for 45 min at room temperature ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... and then circularized using CircLigase II (Epicentre). Two rounds of PCR were then performed to first amplify the library using adaptor-specific primers and to add sequences compatible with Illumina sequencing instruments ...
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bioRxiv - Biophysics 2021Quote: ... TG1 electrocompetent cells were purchased from Lucigen and used for expression of f1pIV for cryoEM studies ...
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bioRxiv - Cancer Biology 2021Quote: ... and RecJ exonuclease (Epicentre #RJ411250) at 30°C for 45 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre). RNA-seq libraries were prepared with SMART-Seq® Stranded Kit (Takara-Clontech) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K) for 45 minutes at RT with light shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... cloni 10G (Lucigen) competent cells respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... and NxGen Rnase Inhibitor (40U/μL; 30281-2 Lucigen). BD Rhapsody cartridges were super-loaded with 60’000 cells each ...
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bioRxiv - Immunology 2021Quote: ... libraries were constructed from first strand cDNA using ScriptSeqTM v2 RNA-Seq library preparation kit (Epicentre Biotechnologies, Madison, WI). Briefly ...
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bioRxiv - Immunology 2021Quote: ... bisulfite-modified DNA sequencing libraries were generated using the EpiGenome kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... heated to 98°C for 5 minutes and cooled to 24°C before use as template for in vitro transcription using the Ampliscribe T7-Flash Transcription Kit (Lucigen). Reactions were allowed to proceed overnight ...
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bioRxiv - Genetics 2021Quote: ... columns and approximately 120–150 ng of DNA was used as template for a T7 in vitro transcription (IVT) reaction (AmpliScribe-T7-Flash transcription kit from Epicentre). In vitro transcribed gRNAs were DNAse-treated using TURBO-DNAse for 20 min at 37 °C and precipitated with sodium acetate/ethanol and resuspended in RNAse and DNAse free water ...
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bioRxiv - Genetics 2021Quote: ... coli (Lucigen, 60242-2) by electroporation (1.8 kV ...
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bioRxiv - Genetics 2021Quote: ... and libraries were constructed using the NxSeq AmpFREE low DNA Library Kit by Lucigen. Bisulfite conversion was done using the EZ-96 DNA Methylation-GoldTM MagPrep kit (D5042 ...
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bioRxiv - Genetics 2021Quote: ... Masterpure Yeast RNA purification kit (Epicentre) was used to extract the RNA by following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and four CS (line 403 subclones 1, S7, S8, and S9) iPSC lines was extracted using quick extract DNA extraction solution (Epicentre #QE09050), amplified by PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was amplified (Epicenter TargetAmp 2-Round aRNA Amplification Kit 2.0, Epicentre Biotechnologies), DNase-treated (RapidOut DNA Removal kit ...
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bioRxiv - Developmental Biology 2021Quote: ... ear clips were lysed by incubation in 25 μl of QuickExtract Solution (QE09050, Lucigen) at 65 °C and shaking at 600 rpm for 30 min ...
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bioRxiv - Genetics 2021Quote: ... Total genomic DNA of hybrids was extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen). DNA concentration was measured using the Qubit dsDNA HS assay (ThermoFischer ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... rRNA was depleted from the purified nsRNA fraction using Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen TER51020) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... and WNT11 Del and LoxP mice mutants was performed by using EconoTaq PLUS GREEN Master mix (Lucigen). The following primers were used for genotyping:
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bioRxiv - Genetics 2021Quote: ... the genomic DNA of the pool of cells carrying insertion events was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Cells were lysed using a lysis solution supplemented in zymolyase 20T (1.5 mg/ml) ...
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bioRxiv - Genetics 2021Quote: ... a restriction digest is performed to exclusively linearize the parental plasmid followed by an overnight digestion with Plasmid-Safe™ ATP-Dependent DNase (Lucigen). Prior to MIJ ...
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bioRxiv - Genetics 2021Quote: DNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... ribosomal depleted using Ribo-Zero Kit (Epicentre) and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) ...
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bioRxiv - Genetics 2021Quote: ... and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre), PCR amplified in 9 cycles ...
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bioRxiv - Genetics 2021Quote: ... cloni 10G supreme (#60081-1; Lucigen). Each library was grown overnight in 500 ml of standard Luria Broth (LB ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Microbiology 2021Quote: ... 100µl of competent cells was mixed with 1 µl of EZ-Tn5 KAN-2 Tnp Transposome (Epicentre) and electroporated at 1800V ...
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bioRxiv - Molecular Biology 2021Quote: ... C-circle and G-circles assays were performed as before except that NxGen® phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified ligation product was transformed into 200 µL of electrocompetent TG1 cells (Lucigen, 60000-PQ763-F) followed by addition of 6 mL of recovery medium (Lucigen ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of 6 mL of recovery medium (Lucigen, F98226-1) and incubation at 37 °C for one hour at 280 rpm rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Developmental Biology 2021Quote: The cell pellets of picked colonies were resuspended in 10 µl QuickExtract (Epicentre, QE0905T) and the suspension was incubated at 65 °C for 10 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries of ribosome protected fragments were generated using the ARTseq™ Ribosome Profiling Kit (Epicentre, RPHMR12126) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Ribosomal RNA was depleted using the Ribo-Zero magnetic kit (Epicentre). Sequencing libraries of ribosome protected fragments were generated using the ARTseq™ Ribosome Profiling Kit (Epicentre ...