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Citations for Lucigen :
1501 - 1550 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... complementary RNA (cRNA) was prepared with the AmpliCap-Max™ T7 High Yield Message Maker Kit (Epicentre Biotechnologies). Oocyte preparation and cRNA injection were performed as previously described [94] ...
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bioRxiv - Biochemistry 2021Quote: ... RNAs were hydrolyzed by addition of 250mM NaOH and the cDNAs were circularized using CircLigase II (Lucigen). The circularized cDNAs were sent to Genomics Core at Scripps Florida for deep sequencing using either single-end or pair-end Illumina NextSeq 500 platform.
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bioRxiv - Biochemistry 2021Quote: ... Purified cDNAs were circularized using CircLigase (50 U; CL4111K, Epicentre) for 2 hr at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U ...
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bioRxiv - Biochemistry 2021Quote: ... coli C43(DE3) cells (Lucigen). Transformed cells were recovered for 45 minutes at 37 °C then and diluted into 200 mL of sterile LB media with the added carbenicillin ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (Lucigen). Cells cultured in SOC media and recovered for 45 minutes at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre, Madison, WI). These pools of barcoded RNAs were converted to Illumina cDNA libraries in three main steps ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Excess linker was degraded by adding 2 μl 10× RecJ buffer (Lucigen), 1 μl RecJ exonuclease (Lucigen) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... The size-selected and purified cDNA was circularized using CircLigase ssDNA ligase kit (Lucigen) following manufacture’s protocol ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μl RNase H buffer and 2 μl hybridase thermostable RNase H (Lucigen) preheated to 45° were added ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1 μl RecJ exonuclease (Lucigen), 1 μl 5′ deadenylase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... Cloning was performed by Gibson assembly (46) using E.cloni 10G cells (Lucigen) and point mutations were performed using the Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA samples were prepared using QuickExtract (Epicentre). The PCR products were column purified and analyzed with Surveyor Mutation Detection Kit (IDT) ...
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bioRxiv - Cell Biology 2021Quote: ... pSMART-HC-Amp plasmid was purchased from Lucigen. pET28-12XHis-SUMO plasmid was from Xudong Wu.
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain TG1 (Lucigen, Madison, WI, USA) or C3040 (NEB) ...
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bioRxiv - Systems Biology 2021Quote: ... and 6,000 U/µl NxGen RNase Inhibitor (Lucigen). Flow rates for the reagent mix were adjusted according to the droplet frequency and size in order to inject the equivalent of ⅓ of the final droplet volume (Movie S3) ...
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bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen) and then cultivated at 37°C in LB medium supplemented with kanamycin and streptomycin ...
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bioRxiv - Systems Biology 2021Quote: ... We then added 3.8 mL of 1x BMg/THS buffer and 0.8 μL of ReadyLyse lysozyme solution (Lucigen), 40 μL of 10 mg/mL RNase A ...
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bioRxiv - Systems Biology 2021Quote: ... genomic DNA was extracted using the MasterPure Complete DNA purification Kit (Lucigen) and sequencing libraries made by both the Nextera XT (FC-131-1024 ...
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bioRxiv - Systems Biology 2021Quote: ... thermophilus HB8 was isolated using the MasterPure Complete DNA purification Kit (Lucigen, Middleton, Wisconsin, US) and used as template for the amplification of the dxs (1-deoxy D-xylulose 5-phosphate synthase ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from all samples using MasterPure Yeast DNA Purification Kit (Lucigen).
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bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
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bioRxiv - Microbiology 2021Quote: ... The vector was transformed into BigEasy-TSA Electrocompetent Cells (Lucigen) for propagation ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Microbiology 2021Quote: ... Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc., Madison, WI, USA) in accordance with the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 U/µl ReadyLyse Lysozyme (Lucigen) in B-PER (ThermoFisher)] ...
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bioRxiv - Microbiology 2021Quote: ... coli (Lucigen) through electroporation (2.5 kV ...
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bioRxiv - Microbiology 2021Quote: ... The reconstructed product OmRV-fragment1/pACYC177 was then transformed into 10G chemically competent cells (Lucigen, E. cloni), propagated for plasmid purification with the aforementioned method ...
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bioRxiv - Microbiology 2021Quote: ... fragment 4 was subsequently subcloned into a low-copy plasmid pSMART LCAmp (Lucigen) to increase stability ...
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bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
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bioRxiv - Microbiology 2021Quote: Ribosomal RNA was removed from strain B50 transcriptomes by using the Ribo-Zero Magnetic Kit (EPICENTRE Biotechnologies ...
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bioRxiv - Genomics 2021Quote: ... end-repaired using End-It™ DNA End-Repair Kit (Lucigen) and A-tailed using Klenow Fragment (3’→5’ exo- ...
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bioRxiv - Microbiology 2021Quote: ... Linear chromosomal DNA was reduced by Plasmid-Safe ATP-Dependent DNase (Epicentre, USA) treatment for 24 hours at 37°C according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and DNA extraction with QuickExtract (EpiCentre) was performed ...
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bioRxiv - Microbiology 2021Quote: ... To extract DNA 10 ul of culture was added to 500 ul QuickExtract Solution (Epicentre) and treated 65 °C for 6 min followed by 98 °C for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
Tnp Transposome (Epicentre) in 50 μL of EV18-pkD46 electrocompetent cells ... -
bioRxiv - Microbiology 2021Quote: ... DNA was extracted using DNA QuickExtract (Lucigen #QE09050). Cells were lysed in 50 μL of QuickExtract solution and incubated at 68°C for 15 min followed by 95°C for 10 min ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... waltii isolates as well as the generated hybrids were extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen).
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bioRxiv - Microbiology 2021Quote: ... coli EPI300 (Lucigen), and E ...
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bioRxiv - Microbiology 2021Quote: ... coli EPI300 cells (TransforMaxTM, Lucigen) and electroporation for transformation highly increased cloning efficiency for the CRISPR-Cas12a constructs in E ...
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bioRxiv - Microbiology 2021Quote: ... and depleted from rRNAs using the Ribo-Zero rRNA removal kit (EpiCentre)59 ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries were generated using a strand-specific ScriptSeq Complete Kit Bacteria protocol (Epicentre), indexed and sequenced on an Illumina HiSeq 2000 platform at the Lausanne Genomics Facilities with 101-nt single-end reads ...
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bioRxiv - Microbiology 2021Quote: ... TSS mapping was performed using an ExactSTAR Eukaryotic mRNA 5’- and 3’-RACE Kit (Epicentre Biotechnologies) as described in the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... resuspended a 0.5 mL ReadyLyse lysozyme solution (Epicentre), and then sample incubated for one hour at 37°C with shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... to decap linear RNA and then was degraded using Terminator 5’-3’ exonuclease (Lucigen). The resulting RNA was put through a size selection step to remove ≤200 nts RNA using SPRI paramagnetic beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... RNaseH (Epicentre) was added to the reaction and incubated at 37°C for 10 min to degrade RNA ...
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bioRxiv - Microbiology 2021Quote: ... 5’PPP structures were then converted into 5’P ends using RNA 5’ Polyphosphatase (5’PP, Epicentre), to which RNA adapters were ligated ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA molecules were depleted from the total RNA samples using the Ribo-Zero rRNA Removal Kit for bacteria (Epicentre), and small RNAs (< 200 nt ...
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bioRxiv - Microbiology 2021Quote: ... with one half being treated with Terminator exonuclease (+TEX, Epicentre), while the other one was left untreated (-TEX) ...
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bioRxiv - Microbiology 2021Quote: ... was synthesized using in vitro transcription (AmpliScribe T7-Flash Transcription Kit, Lucigen) and was then installed with 5’cap (Vaccinia Capping System ...