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Citations for Lucigen :
1101 - 1150 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The supernatant was discarded and approximately 0.2 mL of the sample was incubated with DNases (Promega and Epicentre, Madison, WI, USA), RNase A (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg RNA was incubated with 5 U RNase R (#RNR07250; Epicentre) in 1× RNase R buffer (#RNR07250 ...
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bioRxiv - Molecular Biology 2022Quote: Cell pellets were collected 72-96 hours after electroporation and resuspended in QuickExtract solution (Lucigen, QE09050) and subjected to genomic DNA extraction while incubating for 10 min at 65 °C ...
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bioRxiv - Microbiology 2022Quote: ... the transcripts were dephosphorylated by RNA polyphosphatase (RPP, Lucigen) generating a 5’monophosphate ...
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bioRxiv - Microbiology 2022Quote: RNA from endodontic samples was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre Technologies, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: Endura Electrocompetent Cells (Lucigen #60242-2) were thawed on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... in 1× RNase R buffer (#RNR07250; Epicentre) in a 10 µl reaction volume at 37 °C for 10 min followed by heat inactivation at 95 °C for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted three days after transfection with QuickExtract (QE) (Lucigen). Cells were washed once with PBS and 50 μL QE was added per well of a 96-well plate ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s protocol (Lucigen). Extracted DNA was stored at −20 °C until further use ...
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bioRxiv - Systems Biology 2022Quote: ... RNA was harvested using RNA QuickExtract (Lucigen). Turbo DNAse (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in 0.5 X PBS-RI and resuspended in 75 μL of 1X Ampligase buffer (Lucigen, A0102K). Pooled cells were counted and examined on the hemocytometer and diluted for loading onto the Chromium Controller (10x Genomics) ...
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bioRxiv - Neuroscience 2022Quote: cDNA was made from 200 ng of labellar RNA as template from using EpiScript (Lucigen). Two biological replicates were prepared per species ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was harvested with QuickExtract (Lucigen) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.1% NxGen RNAse inhibitor (Lucigen; 30281-2)) ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 8-well strips containing 3 μL of cell collection buffer (0.1% Triton X-100, 0.2 U/μL RNAse inhibitor (Lucigen)) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1 μL 40 U/μL RNasin (Lucigen), and 0.6 μL nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and NxGen RNase inhibitor (0.2U/uL; Lucigen). NAc tissues were dissected rapidly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... were ligated to the ChIP DNA (Fast-Link kit; Epicentre) and PCR amplified using the Illumina TruSeq ChIP Library Prep Kit ...
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bioRxiv - Neuroscience 2022Quote: ... coli (Lucigen 60242-2) using program EC1 on MicroPulser Electroporator (Bio-Rad 1652100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2000 K U/µl Ready-Lyse lysozyme (Epicentre)) and incubated at 37°C for 30 min to degrade the cell wall ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared with ScriptSeq v2 RNA-seq library preparation kit (Epicentre) and sequencing was performed on NextSeq500 (single-end 96 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented gDNA was ended-repaired in 50 μl reaction using the End-It DNA End-Repair Kit (Lucigen, ER81050), incubated at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted using Quickextract solution (Lucigen). A ∼700 bp-1 kb region flanking the sgRNA annealing site was amplified by PCR 5-7 days after RNP nucleofection ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 2000 K U/µl Ready-Lyse lysozyme (Epicentre)) and incubated at 37°C for 30 min to degrade the cell wall ...
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bioRxiv - Microbiology 2022Quote: ... 5 U Baseline-ZERO DNase (Epicentre), 25 U Benzonase (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μg of RNA was treated with a beta version of Ribo-Zero Magnetic Gold Kit Yeast (Epicentre) to deplete rRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 1 μg purified RNA was applied to RT-qPCR and the remaining was subjected to ribosomal RNA depletion using a Ribo-Zero Gold Kit (Epicentre). Sequencing libraries were constructed from 100 ng total RNA using a Truseq stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
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bioRxiv - Microbiology 2022Quote: ... and the target exons were amplified with EconoTaq polymerase (Lucigen, Middleton, WI, USA). The resulting amplicons were subcloned using the TOPO TA Cloning Kit for Sequencing (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... a PlasmidSafe DNase (PSD; Epicentre) experiment was performed as described previously [5] ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was extracted using QE buffer (Lucigen) and PCR was performed using Q5 polymerase (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Molecular Biology 2022Quote: Ribosomal RNAs (rRNA) were removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Madison, WI). For the whole biopsies ...
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bioRxiv - Microbiology 2022Quote: ... depleted in ribosomal RNA (Ribo-Zero rRNA Removal Kit, Epicentre) and reverse transcribed into cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Cell Biology 2022Quote: ... genomic DNA was extracted using the QuickExtract DNA extraction kit (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... TypeOne™ Restriction Inhibitor (Lucigen), EZ-Tn5
transposon (at 0.1 pmol/μL ... -
bioRxiv - Genomics 2022Quote: ... 1 U/μL RNase Inhibitor (NxGen, Lucigen), 1 U/μL reverse transcriptase (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted with the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, Cat. No.: MC85200). The Qubit 3.0 fluorometer and Nano-300 (Allsheng™ ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...