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Citations for Lucigen :
951 - 1000 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... were sent to BGI (Hongkong) for ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) followed by library construction using non-stranded (Replicate 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1.2ug of ligated pUSEPR plasmid DNA per sub-pool was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli cells (Lucigen, Middleton, WI) electroporated with Rmona658B genomic DNA that had been linearized by digestion with SmaI and ligated into the linear vector pJAZZ (Lucigen) ...
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bioRxiv - Microbiology 2022Quote: ... electroporated with Rmona658B genomic DNA that had been linearized by digestion with SmaI and ligated into the linear vector pJAZZ (Lucigen). The resulting construct was termed pJAZZ[pRM658B] (Fig ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Molecular Biology 2022Quote: ... 30 μg of lysate was diluted to 200 μL in polysome buffer (lysis buffer without Triton X-100) and 1.5 μL RNase I was added (Epicentre #N6901K). RNase digestion was carried out at 200 rpm at 37°C for 45 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were circularized using circligase II (#CL9025K, Epicentre). The major rRNA contaminants were removed using subtractive hybridization with custom biotinylated oligos (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reverse complement DNA templates were ordered from Integrated DNA Technologies (USA) and transcribed using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen). The DNase treated products were cleaned with Quick-RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Biophysics 2022Quote: ... The product was then packaged into phage particles using phage extract (MaxPlax, Epicentre). Plaques were generated on LE392 E ...
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bioRxiv - Biophysics 2022Quote: ... coli bacterial lawns (Epicentre) and screened for the ARS1 insert ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted following the protocol amended from Epicentre MasterPure™ DNA Purification Kit (http://homings.forsyth.org/DNA%20Isolation%20Protocol.pdf) ...
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bioRxiv - Cell Biology 2022Quote: The MasterPure™ Complete DNA and RNA Purification kit (Epicentre, MC85200) or the RNeasy kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA of expanded clones was isolated using QuickExtract DNA extraction solution (Lucigen, QE0905T). PCR was performed by amplifying a 241bp area surrounding the mutation site ...
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bioRxiv - Genomics 2022Quote: ... Repair ends of DNA using End-It DNA End-Repair Kit (Lucigen, Cat. ER0720), then perform A-tailing with Klenow Fragment (3’-> 5’ exo- ...
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bioRxiv - Biochemistry 2022Quote: ... coli competent cells (Lucigen). A single colony of transformed cells was grown in 3 mL lysogeny broth (LB ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 ng each sample was subjected to end-repair (End-It DNA End-Repair kit, Lucigen), A-tailing (Klenow fragment (3’-5’ exo– ...
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bioRxiv - Genomics 2022Quote: DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen), depending on confluency 25 to 100 μl of solution was added to the wells containing the cells for 15 minutes at 37C ...
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bioRxiv - Genomics 2022Quote: ... and EconoTaq PLUS GREEN 2X Master Mix (Lucigen) in a 25 μl reaction ...
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bioRxiv - Biochemistry 2022Quote: ... Clones with the best response to TMP were expanded and lysed for gDNA extraction and purification using the QuickExtract DNA extraction solution (Lucigen, Middleton, WI). Genomic ecDHFR was amplified (forward ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed once with DPBS and genomic DNA (gDNA) was extracted with 50 µL/well of Quick-Extract solution (Lucigen, QE09050). Lysates were pipetted up and down thoroughly ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted with QuickExtract DNA extraction solution (EpiCentre) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... DNA was prepared using the MasterPure™ Complete DNA and RNA purification kit (Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI ...
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bioRxiv - Biochemistry 2022Quote: ... coli TG1 (Lucigen) in 2xYT medium (yeast extract 1% w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 10pmol of template oligo was cyclized and ligated using CircLigase II ssDNA Ligase (Lucigen) supplemented with 2.5mM MnCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3) Remaining linear DNA was removed by exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre), assisted by rare-cutting endonuclease MssI (only support Homo sapiens ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:200 diluted endonuclease (Lucigen: OC7850K) was immediately added to the pulverized tissue at a constant ratio of 15μL/mg of tissue ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA extractions were performed using MasterPure™ RNA Purification Kit (Epicentre®) according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from 2ml of cells at OD595 = 0.8-1.0 using the Masterpure Yeast RNA Purification Kit (Lucigen). Small RNAs were extracted by resuspending 50ml of pelleted cells at OD595 = 0.8-1.0 in 50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2022Quote: ... or Epi400 (Lucigen). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloning was carried out by adding the two 15kb fragments (80fmol each) to 40fmol pSMART BAC vector (Lucigen) and incubating them with a proprietary assembly mix (Twist ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Synthetic Biology 2022Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen) were transformed with each library and expressed in a 96-well deep well format.21 General methods for liquid handling and fluorescence plate reader screening at pH 5 ...
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bioRxiv - Plant Biology 2022Quote: Five micrograms of RNA isolated from enriched chloroplasts was digested with 6U of Terminator exonuclease (Lucigen catalog number TER51020) for 1h at 30°C in 20µl reaction volume ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... genomic DNA from single colonies was extracted with the MasterPureTM DNA Purification Kit from Epicentre (Cat. No. MCD85201) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... two µg of total RNA were treated with 5 U of RNase R (cat. # RNR07250, Lucigen, Middleton, WI) for 40 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... The resulting air-dried pellets were resuspended in RNase-free water and 3 µg of DNA from each sample was depleted of ribosomal RNA using the Bacteria Ribo-Zero rRNA Removal Kit (Epicentre) and purified using the RNeasy miniprep kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Clonal populations were genotyped by Sanger sequencing the PCR amplified sgRNA-targeted sites in the gDNA extracted using DNA QuickExtract (Lucigen). Resulting sequences were compared to references and clones containing a frameshift indel or de novo stop codon were selected ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from bacterial cell pellets using MasterPure™ complete DNA purification kit (Lucigen). Oxford Nanopore Minion Sequencing ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was removed using a Ribo-Zero rRNA removal kit (Epicentre; Madison, WI). Gene expression was quantified through the Kallisto software (Bray et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA from each colony was harvested with QuickExtract DNA Extraction (Lucigen, QE09050) and then used for PCR amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligo pools were cloned into a modified pLKO-TET-ON plasmid by the Golden Gate method and the resulting product was used to transform Endura electrocompetent cells (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: Equal amounts of RNA (2µg) were incubated with or without 20 U of Rnase R (RNR07250, Epicentre Biotechnologies) and 40 U of RiboLock RNase inhibitor (EO0381,Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... rRNA depleted islet RNA was digested using RNase R (RNR07250, Lucigen) or mock digestion (protocol without RNase R ...
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bioRxiv - Genomics 2022Quote: ... Sonicated gDNA ends were repaired using End-It™ DNA End-Repair Kit (Epicentre) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... high molecular weight DNA was isolated using the MasterPure DNA Purification kit (Epicentre, no. MC85200). Sequencing was performed using the PacBio Sequel (Pacific Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) containing 50 U/μL Ready-Lyse Lysozyme (Lucigen #R1804M) and protease inhibitors ...