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Citations for Lucigen :
901 - 950 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from cell pellets with QuickExtract DNA Extraction Solution (Lucigen) via manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted by using the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI) as described by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... The genomic DNA was extracted from homogenized liver tissues using the MasterPure Complete DNA and RNA Purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was repaired with Blunt-end ending 3′ overhang using End-It DNA End-Repair kit in 25 µl volume (Epicentre; ER81050), and 3′-A overhang was added with Klenow fragment (NEB ...
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bioRxiv - Physiology 2023Quote: ... coli CopyCutter (Lucigen, C400CH10). The sequence was verified using sequencing of the full coding sequence.
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bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Microbiology 2023Quote: Tn-5 transposon insertion library was build based on EZ-Tn5™
Tnp transposome system (Lucigen, WI, USA). Competent C ... -
bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Microbiology 2023Quote: Phage DNA was isolated using the MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA) with modifications ...
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bioRxiv - Microbiology 2023Quote: ... coli C43 (DE3) electro-competent cells (Lucigen) modified to incorporate a pspA gene knockout60 ...
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bioRxiv - Molecular Biology 2023Quote: ... After treatment with Rnase A (Epicentre) at 37°C for 20 minutes and Proteinase K (Roche ...
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bioRxiv - Immunology 2023Quote: ... 10 µl of the reaction were electroporated in 50 µl Lucigen Endura electro competent cells (Lucigen, #602421) using 1 mm electroporation cuvettes (Biorad ...
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bioRxiv - Immunology 2023Quote: ... Circular ligation was performed using CircLigase II ssDNA Ligase (Epicentre CL4111K) for 1,5 hours at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 72 µL of either Hybridase Thermostable RNase H (Lucigen H39500) or Thermostable RNase H (NEB M0523 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was maintained in 10G elite Escherichia coli bacteria (Lucigen).
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM LiCl ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2U/uL RNAse inhibitor (Lucigen). The tissue was then triturated 15 times through a fire-polished Pasteur pipette and then passed through a 40 uM filter to remove cell debris and large chunks ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... into pSMART-HC-Kan (Lucigen) vectors along with upstream CMV promoters and downstream IRES2-SYFP2 or IRES2-mScarlet transfection reporters for expression in HEK-293 cells ...
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bioRxiv - Neuroscience 2023Quote: ... To minimize rearrangement of full length SCN1A we used pSMART-HC-Kan backbone (Lucigen) which helps minimize backbone-driven transcription in bacteria ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 μL of Hybridase RNase H (Lucigen H39500), 3 μL of 5× RNase H buffer (167 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (E. cloni 10G Elite, Lucigen, USA). Further details on the microfluidic screening can be found in the Supplementary Information.
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bioRxiv - Microbiology 2022Quote: ... coli (Lucigen). Transformations were immediately recovered in SOC medium at 30°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was precipitated using ispopropanol and contaminating RNA removed using RiboShredder™ RNase Blend (Epicentre). RNase was removed using an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was extracted using the Masterpure Complete DNA Purification Kit (Lucigen-Biosearch Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... coli cells (Lucigen cat. no. 60242-1) using prechilled 1 mm electroporation cuvettes (BioRad cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNAs were prepared using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre). Second ...
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bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) and purified as described by Urbanek et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G SOLO cells (Lucigen). Whole transformations were plated on LB-agar + 25 μg/mL chloramphenicol and grown overnight ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cloni 10G cells (Lucigen, USA). Cells were grown overnight at 37 °C on LB-agar + 50 μg/mL kanamycin plates and transformants scraped for plasmid DNA isolation ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 U RecJ exonuclease (Epicentre). Up to 8 libraries were pooled ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with 500 U of RNAse I (Epicentre) per 25 μg of RNA (quantified by Qubit fluorimetry – ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs were circularized using 100U CircLigase I ssDNA ligase (Epicentre), and cDNA concentration was quantified by QPCR of cDNA compared to a standard curve of a reference sample ...
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bioRxiv - Cell Biology 2022Quote: ... coli 10G SUPREME electrocompetent cells (Lucigen). This plasmid pool was used to produce lentivirus-containing supernatants in HEK293T cells ...
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bioRxiv - Immunology 2022Quote: ... coli C41 OverExpress™ C41(DE3) (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) were transformed with expression plasmid and a single colony was used to start an overnight culture in LB media ...
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bioRxiv - Immunology 2022Quote: ... ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre ...
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bioRxiv - Neuroscience 2022Quote: ... and A-tailed (Klenow Exo-minus; Epicentre). Adapters (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... coli bacterial strain (Lucigen). Expression was induced for 1h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by treatment by a terminator exonuclease (Epicentre), dephosphorylation by Calf Intestine Phosphatase (NEB ...