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Citations for Lucigen :
501 - 550 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis and strand-specific library generation was carried out using the Script-Seq V2 Kit (Epicentre) and the FailSafe PCR enzyme mix ...
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bioRxiv - Genomics 2023Quote: ... with the following modifications as previously published37: Swabs were placed in tubes containing 350 ul Tissue and Cell Lysis solution (Lucigen), 1.5 mg/ml lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... then resuspend in 1mL resuspension buffer (20 mM Tris-HCl pH 7.5, 300 mM NaCl, 12500 U Ready-lyse (Epicentre technologies), and one tablet of EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage selections were amplified by adding them to 1.0 ml TG1 cells (Lucigen) grown to mid-log phase (OD ∼ 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified cDNA was then circularized using CircLigaseII ssDNA ligase (Epicentre) for 1[h at 60°C and was subsequently PCR amplified using the primers 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC-3′ and 5′-CAAGCAGAAGACGGCATACGAGATJJJJJJGTGACTGGAGTTCAGACGTGTG-3′(where Js indicates the 6-mer index sequence for Illumina sequencing).
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bioRxiv - Microbiology 2023Quote: ... ribosomal RNA was removed using the Ribo-Zero rRNA removal kit for Gram-negative bacteria (Epicentre/Illumina), and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina) (76) ...
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bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen #60242-1) using a Gene Pulser Xcell (Bio-Rad #1652662) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen, Middelton, WI, USA). 50 ng of genomic DNA were used to amplify the region that spans the cutting site of each gRNA using KAPA2G Fast ReadyMix (Kapa Biosystem ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using the MasterPure Yeast DNA purification kit (Epicentre, MPY80200), and concentrations were measured by Qubit ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl of RNase inhibitor (Lucigen) and 450 μl of IP buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... and excess unligated linker was removed using 10 U/µl 5’ deadenylase/RecJ exonuclease (Epicentre, RJ411250), followed by pooling and purification of ligated footprints using an Oligo Clean and Concentrator column (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA (gDNA) was extracted by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Microbiology 2023Quote: ... Samples were collected using sterile Catch-All™ Specimen Collection Swabs (Epicentre Biotechnologies, Madison, WI), and placed in MoBio bead tubes with buffer (MoBio PowerSoil™ kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Single clones were expanded and genotyped for the desired knock-in by PCR on genomic DNA extracted with Quickextract solution (Lucigen). Clones showing biallelic knock-ins were expanded and frozen in LN2 at early passages ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using to MasterPure Yeast RNA Purification kit protocol (Epicentre) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Plant Biology 2023Quote: ... coli [C41 pLysS (Lucigen, USA)] by cloning their coding sequences in pET28a(+ ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5% SDS) and purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, WI, USA). RNA was prepared by TRizol according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 1U of RNase H (Hybridase Thermostable RNase H, Epicentre), purified using RNA Cleanup XP beads (Agencourt) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C43 (DE3) cells (Lucigen) while VLCADWT (res ...
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bioRxiv - Genomics 2023Quote: ... The MDA reactions were prepared using RepliPHI Phi29 DNA polymerase and Reagent set (Epicentre, Illumina, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 250 pmol of Qβ-RNA were treated with 60 U of RNA 5’-polyphosphatase (Epicentre) in 1 x polyphosphatase reaction buffer at 37 °C for 70 min ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA were extracted using QuickExtract DNA Extraction Solution (Epicentre). PCR amplification products of the mutation site were used in restriction fragment length polymorphism assays with the AluI restriction enzyme (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... cloni 10G (Lucigen) was used for library construction ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... TransforMaxTMEPI300TM Electrocompetent Escherichia coli (Lucigen) was transformed with these replicons using MicroPulser™ (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Cloning into CopyControl™ pCC2Fos™ (Lucigen, Middleton, Wisconsin, USA) was conducted according to the manufacturer’s protocol using linker-connected replicon DNA as an insert.
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 U/ml RNase I (Lucigen, N6901K) for 30 minutes at room temperature with gentle mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells from each of these 4 wells were dissociated and divided into 2 portions–one portion was used to collect genomic DNA using QuickExtract DNA Extraction solution (Lucigen) while 500 cells from the other portion was suspended in 25 ml of culture medium and 100 ul was seeded into each well of two 96-well plates for further culture ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA was obtained by using QuickExtract DNA Extraction Solution (Lucigen), where 10000 cells were resuspended in 100 μl PBS ...
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bioRxiv - Microbiology 2023Quote: ... transformed into TransforMax EPI300 electrocompetent cells (Lucigen) by electroporation ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were thawed and RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) followed by DNase treatment with the DNase TURBO DNA-free kit (Invitrogen AM1907) ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in a Ready-Lyse lysozyme solution (Epicentre Technologies) according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted from yeast pellets using the MasterPure™ Yeast RNA Purification Kit (Lucigen Cat. No. MPY03100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Directional libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and single end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worcester ...