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Citations for Lucigen :
401 - 450 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Recombinant human apo-A1 (pET20b apo-A1) was over-expressed in ClearColi BL21 (DE3) (Lucigen). Upon reaching OD600 0.7-0.8 ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cells by resuspending the pellet in 250 µL of QuickExtract DNA Extraction Solution (Lucigen, QE09050). Samples were heated at 65°C for 6 minutes and 98°C for 2 minutes before amplifying the region surrounding the CRISPR cut site by PCR ...
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bioRxiv - Bioengineering 2024Quote: Cells were lysed with QuickExtract (QE0905T, Lucigen) and gDNA was purified using SPRI magnetic bead cleanup.
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from two biological replicates from early log phase growth in either YPD or YPD containing 5 mM TUDCA using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... introduced into Endura Electrocompetent DUO cells (Lucigen) by electroporation ...
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bioRxiv - Cell Biology 2024Quote: RNAs from cells (2,000 ng) and EVs (960 ng) was incubated with 1 U/µg RNase R (Lucigen, RNR07250) at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL RNAse A (Lucigen) was added to the cells and incubated for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and samples were circularized with CircLigase I (Lucigen). A round of rDNA depletion was performed using biotin-labeled antisense probes and Dynabeads MyOne Streptavidin C176 ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was harvested from clonal cell lines using QuickExtract (Lucigen, QE09050) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Genomic DNA was extracted using QuickExtract DNA extraction solution (Epicentre). Target regions were amplified by PCR with HOT FIREPol® DNA Polymerase (Solis BioDyne ...
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bioRxiv - Biochemistry 2024Quote: ... coli C43 (DE3) cells (Lucigen), cultured in LB medium supplemented with 100 μg/mL ampicillin at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... intermedia ATCC 25611 was extracted with MasterPureTM complete DNA and RNA purification kit (Lucigen Corp, Middleton, WI, USA) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting DNA was transformed to an electro-competent ER2738 E.coli strain (Lucigen, 60522-1) and cultured on 2YTG (2% glucose ...
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bioRxiv - Biochemistry 2024Quote: ... was cloned in the pRhamTM N-His SUMO Kan vector (Lucigen) following manufacturers’ recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL of purified DNA was transformed into 25 μL of Endura electrocompetent cells (Lucigen) following the manufacturer’s instructions with 2 mL of prewarmed recovery media ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL of purified DNA was transformed into 25 μL of Endura electrocompetent cells (Lucigen) following the manufacturer’s instructions with 2 mL of prewarmed recovery media ...
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bioRxiv - Biochemistry 2024Quote: ... the expression was conducted in the E.coli strain C41 (DE3) pLysS (Lucigen) in LBE-5052 autoinduction medium for 24 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... circularized using CircLigase (Epicentre, CL4115K), and PCR amplified (8–12 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Solutions were washed in binding buffer and aspirated on a magnetic plate three times before being resuspended in a PCR master mix (Lucigen Econo Taq 2X 30035-1 and custom primers) ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified reactions were electroporated into Endura DUOs (Lucigen) using a MicroPulser with program Ec1 (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µl End-Repair Enzyme Mix (Lucigen,14422-5). Incubated at room temperature for 45 mins and purified using the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2024Quote: We transformed the assembled plasmid libraries via electroporation into Endura electrocompetent cells (Lucigen). We performed five electroporations ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA for genotyping was extracted using QuickExtract DNA Extraction Solution (Lucigen, QE09050) from harvested yolk sac tissue ...
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bioRxiv - Cell Biology 2024Quote: ... washed with PBS and resuspended in 50 μl QuickExtract (Lucigen) at 65 °C for 20 min and 98 °C for 5 min ...
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bioRxiv - Genetics 2024Quote: ... The supernatant was discarded and 1.5mL Buffer D (0.01% BSA in 1X PBS with 0.5U/uL RNase inhibitor [Lucigen 30281-2]) was gently added to the nuclei pellet and incubated on ice 20min ...
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bioRxiv - Cell Biology 2024Quote: ... for Human cell lines and by a by a hybridization method using Ribo-ZERO kits (Illumina-EpiCentre for Zebrafish samples ...
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bioRxiv - Cell Biology 2024Quote: ... an aliquot of cells was used to extract the DNA by QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). To assess the genomic editing efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... or QuickExtractTM DNA Extraction Solution (Lucigen, QE09050). A 601 bp genomic region (chr11:92,975,259- 92,975,860 ...
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bioRxiv - Genetics 2024Quote: ... Up to 100 ng of purified ligation product was electroporated into Endura electrocompetent cells (Lucigen) using a Gene Pulser XCell instrument (Biorad) ...
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bioRxiv - Genetics 2024Quote: ... samples were further treated with RNA 5′- polyphosphatase (Epicentre) as previously described[26].
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bioRxiv - Genetics 2024Quote: ... they were further expanded and gDNA was harvested using Quick Extract DNA Extract Solution (Lucigen, QE9050). Excision and inversion clones were genotyped via ddPCR and Sanger sequencing (Table S66).
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bioRxiv - Genetics 2024Quote: ... cloni 10GF’ ELITE Electrocompetent Cells (Lucigen) and grown in 500 mL of LB-Ampicillin media at 30°C overnight and bacterial pellets were frozen at -80°C ...
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bioRxiv - Genetics 2024Quote: ... and end-repaired using the End-it DNA End-repair kit (Epicentre Technologies). Common adaptors from the Multiplexing Sample Preparation Oligo Kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... To extract gDNA the pellet was resuspended in 50μL QuickExtract (Lucigen). Bacterial suspensions were then heated to 65°C for 6 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... rRNA was removed using a Ribo-Zero kit (Epicentre, Madison, WI) and mRNA was randomly fragmented ...
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bioRxiv - Cell Biology 2024Quote: ... The purified plasmid library was electroporated into Endura Electrocompetent cells (Lucigen) and grown at 30 °C for 16 h on agar with 100 ug/mL carbenicillin ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1×107 mid-log phase cells using the Masterpure Yeast RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The library was transformed into electrocompetent cells (Lucigen 60242-1) and recovered at 32°C for 16-18 hours to prevent recombination ...
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bioRxiv - Cell Biology 2024Quote: ... The library was transformed into electrocompetent cells (Lucigen 60242-1) and recovered at 32°C for 16-18 hours to prevent recombination ...
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bioRxiv - Genetics 2024Quote: ... The collected cells were subjected to DNA extraction using QuickExtract buffer (Lucigen), following manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... and RNA was extracted using MasterPure™ RNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... sing1 gDNA was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen, MPY80200). Total RNA was extracted with TRIzol™ (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Double stranded RNA (dsRNA) were produced by in vitro synthesis reactions (NxGen, Lucigen) using primers listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: The electroporated progenitors were subjected to adipogenic differentiation for 8 days and the genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen QE09050). PCR reactions were conducted with 100 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche KK2602 ...
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bioRxiv - Immunology 2024Quote: ... coli TG1 (60502, Lucigen, Middleton, WI, USA) were then transformed with VHH sequence harbouring pMECS vectors ...
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bioRxiv - Biochemistry 2024Quote: ... coli (Lucigen). Starter cultures were inoculated from an individual colony on a C43(DE3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then poly(A)-tailed using poly(A) polymerase and the 5’-PPP was removed using 5’ polyphosphatase (Epicentre). RNA adaptors were then ligated and synthesis of first strand cDNA was performed using M-MLV reverse transcriptase and oligo(dT ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...