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Citations for Lucigen :
401 - 450 of 2386 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated for 72h and harvested with Quick Extract (Lucigen). Genomic DNA was amplified and sequenced as described above.
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bioRxiv - Evolutionary Biology 2023Quote: ... and 100 mM NaCl) and then lysed the pelleted cultures with 250U/μL lysozyme (Ready-Lyse Lysozyme Solution; Lucigen R1804M) at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... extractions were performed using the MasterPure yeast DNA purification kit (Epicentre). Genomic libraries ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the isolates using the MasterPure™ Gram positive DNA extraction kit (Cat. No. MGP04100; Epicentre®). The standard protocol was modified slightly to accommodate for the isolates being Gram negative organisms ...
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bioRxiv - Cell Biology 2023Quote: ... Ruptured cells were treated with 1 U/μl OmniCleave endonuclease (Lucigen, OC7850K) for 5 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by addition of 50 μl QuickExtract DNA solution (Epicentre, QE09050) to the cells ...
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bioRxiv - Cell Biology 2023Quote: The electroporated progenitors were subjected to adipogenic differentiation for 8 days and the genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen). PCR reactions were conducted with 50 ng of genomic DNA and following primer pairs spanning the sgRNA target site in KAPA HiFi HotStart ReadyMix (Roche ...
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bioRxiv - Immunology 2023Quote: ... coli TG1 cells (Lucigen), where the uracilated strand is cleaved in vivo by uracil N-glycosylase ...
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bioRxiv - Immunology 2023Quote: The Escherichia coli strains TG1 and AXE688 (21, 22, 23) were purchased from Lucigen Corporation (Middleton ...
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bioRxiv - Immunology 2023Quote: ... coli TG1 (Lucigen Middleton, WI) for 30 min at 37 °C ...
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Transcriptional dissection of symptomatic profiles across the brain of men and women with depressionbioRxiv - Neuroscience 2023Quote: ... RNA libraries were synthesized from 1 μg of RNA using the ScriptSeq Complete Gold Kit (Epicentre, Illumina) including an initial ribosomal RNA depletion step ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Bioengineering 2023Quote: ... coli 10G Chemically Competent Cells (Lucigen) were transformed with the ligation mixtures and plated on LB plates containing 50 µg kanamycin (LB-Kan ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was harvested using QuickExtract DNA Extraction Solution (Lucigen), and gDNA was prepared by heating to 65°C for 10 min followed by heat inactivation at 95°C for 2 min ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated with 100 μL QuickExtract solution (Lucigen CorporationSupplier Diversity Partner QuickExtract DNA Extraction Solution 1.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli EPI300 (F’ λ - mcrA Δ(mrr-hsdRMS-mcrBC) ϕ 80dlacZ ΔM15 Δ(lac)X74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) nupG trfA dhfr) (Epicentre) as well as a diaminopimelic acid (DAP ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid pETite (Lucigen) containing the recombinant VHL (M54 – D213 ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted using QuickExtract (Lucigen) and the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated using QuickExtract (Epicentre), incubated at 65°C for 6 minutes followed by an incubation at 95°C for 2 minutes using a thermal cycler ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoid DNA was extracted with QuickExtract™ DNA Extraction Solution (Lucigen) and PCR amplified ...
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bioRxiv - Biophysics 2023Quote: ... coli (Lucigen). Cultures were grown in 2xYT medium supplemented with 50 μg/ml kanamycin at 37°C until an OD600 of ∼1.5 was reached ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by reaction clean up with exonuclease I (Lucigen, 37C for 30 minutes), RNase I/Hybridase (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA pellets were washed once in 80% ethanol and pellets were air-dried and dissolved in 11uL of water, which was then circularized with the addition of 9uL of CircLigase I mix (1M betaine, 1x CircLigase Buffer (Lucigen), 2.5mM MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5U/uL CircLigase I (Lucigen)) at 60C for 3 hours with heat inactivation at 80C for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was generated with EpiScript RT enzyme (Lucigen, 50C for 30 minutes) followed by reaction clean up with exonuclease I (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to end-repair with T4 PNK (Lucigen, 37C for 1 hr). End-repaired RNA was cleaned up with the RNA Clean and Concentrator kit (Zymo) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligated pUSEPR plasmid DNA was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37⁰C ...
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bioRxiv - Immunology 2023Quote: ... The cDNA synthesis was performed within each well of a 96-well plate separately using EpiScript™ Reverse Transcriptase (EpiCentre Biotechnologies) using UMI-tagged oligo-dT primer with universal tail (Suppl ...
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bioRxiv - Microbiology 2023Quote: ... A 5 µg aliquot of total RNA was depleted for rRNA with the mRNA then converted into cDNA with the Scriptseq complete bacteria kit (Epicentre). The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s instructions for DNA isolation ...
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bioRxiv - Microbiology 2023Quote: Transposon library of Salmonella was generated using EZ-Tn5
Tnp Transposome kit (Lucigen, TSM99K2) following manufacturer’s instructions ... -
bioRxiv - Microbiology 2023Quote: High molecular weight DNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. Seven days old-mycelia of P ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using a MasterPure Gram Positive DNA Purification Kit (Epicentre, MGP04200) according to the manufacturer’s instructions or ...
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bioRxiv - Microbiology 2023Quote: ... 1mL of cell culture was collected and used for genome DNA extraction using Lucigen MasterPure Complete DNA & RNA with additional RNase step (Lucigen, Mandel Scientific, Guelph, ON, Canada) following the manufactures’ manual.
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bioRxiv - Plant Biology 2023Quote: ... a modified PET32a vector with an N-terminal His6-SUMO expression tag and SUMO protease cleavage site (Lucigen Corporation). SALTY fusion was expressed in Codon+ BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (Lucigen). Integration ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
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bioRxiv - Microbiology 2023Quote: ... following an adapted version of the manufacturers’ protocol including an additional step of adding 0.5 µL of Ready-Lyse™ Lysozyme Solution (Lucigen). Next ...
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bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen), and recovered for one hour at 37°C in Recovery Media (Lucigen) ...
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bioRxiv - Microbiology 2023Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, cat. CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre) and 0.5% SDS (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... The CopyControl™ Fosmid autoinduction solution was from Lucigen Corporation (Middleton ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA contamination was depleted using Baseline-Zero DNase (Lucigen/Epicentre, #DB0715K) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The purified product was electroporated into Endura competent cells (Lucigen). We extracted the plasmid pool using the Plasmid Maxi prep kit ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 500 μL of Red blood cell lysis buffer (Lucigen, SS000400-D2) and incubated at room temperature for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... either into pCC1FOS™ plasmid (Epicentre, USA) for attB libraries ...