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Citations for Lucigen :
451 - 500 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coli C43 (DE3) cells (Lucigen) while VLCADWT (res ...
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bioRxiv - Genomics 2023Quote: ... The MDA reactions were prepared using RepliPHI Phi29 DNA polymerase and Reagent set (Epicentre, Illumina, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 250 pmol of Qβ-RNA were treated with 60 U of RNA 5’-polyphosphatase (Epicentre) in 1 x polyphosphatase reaction buffer at 37 °C for 70 min ...
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bioRxiv - Neuroscience 2023Quote: QuickExtract™ DNA Extraction Solution (QE09050, Lucigen) was used to extract gDNA from the subclones for PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA were extracted using QuickExtract DNA Extraction Solution (Epicentre). PCR amplification products of the mutation site were used in restriction fragment length polymorphism assays with the AluI restriction enzyme (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... cloni 10G (Lucigen) was used for library construction ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... TransforMaxTMEPI300TM Electrocompetent Escherichia coli (Lucigen) was transformed with these replicons using MicroPulser™ (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... Cloning into CopyControl™ pCC2Fos™ (Lucigen, Middleton, Wisconsin, USA) was conducted according to the manufacturer’s protocol using linker-connected replicon DNA as an insert.
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 U/ml RNase I (Lucigen, N6901K) for 30 minutes at room temperature with gentle mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells from each of these 4 wells were dissociated and divided into 2 portions–one portion was used to collect genomic DNA using QuickExtract DNA Extraction solution (Lucigen) while 500 cells from the other portion was suspended in 25 ml of culture medium and 100 ul was seeded into each well of two 96-well plates for further culture ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA was obtained by using QuickExtract DNA Extraction Solution (Lucigen), where 10000 cells were resuspended in 100 μl PBS ...
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bioRxiv - Microbiology 2023Quote: ... transformed into TransforMax EPI300 electrocompetent cells (Lucigen) by electroporation ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were thawed and RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) followed by DNase treatment with the DNase TURBO DNA-free kit (Invitrogen AM1907) ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed in a Ready-Lyse lysozyme solution (Epicentre Technologies) according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted from yeast pellets using the MasterPure™ Yeast RNA Purification Kit (Lucigen Cat. No. MPY03100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Directional libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and single end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worcester ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC vector that can handle the full-length genome was purchased from Lucigen (cat # 42032-1). This vector was modified to include a CMV promoter ...
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted again to 400 mL of LB30 medium + 12.5 μg/mL of chloramphenicol + 1x Arabinose induction solution (Lucigen) for overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The ribosomal RNA was removed from the purified RNAs using the Ribo-Zero magnetic kit (Epicentre). The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre) ...
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bioRxiv - Microbiology 2023Quote: ... Successful RppH treatment was verified by incubating 100 ng of 5’P or 5’PPP transcripts with 20 U RiboLock and 1 U Terminator™ 5’Phosphate-Dependent Exonuclease (Lucigen) in 1x TEX Buffer B for 30 min at 42°C ...
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bioRxiv - Microbiology 2023Quote: ... unligated 5’-P RNA fragments were removed using terminator exonuclease (TEX, Lucigen), followed by ligation of transcriptional start site (TSS)-specific adaptors.
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli DH5α or CopyCutter EPI400 (Lucigen) was used for cloning and grown in Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Genetics 2023Quote: ... DNA were extracted using MasterPure yeast DNA purification kit (Epicentre, MPY80200). ChEC DNA was subjected to size selection using the Pippin Prep (SageScience ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen) were transformed with 2.5 µl of the eluate each ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were sorted in 5 μl of QuickExtract DNA Extraction Kit (Epicentre, USA) in 96-well format.
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bioRxiv - Neuroscience 2023Quote: 1 µg of BH RNAs was incubated with 1 U/µg RNase R (Lucigen RNR07250) at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was done by using the MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) to gain high quality and quantity DNA for metagenomic sequencing ...
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... and residual linear DNA was degraded by Plasmid-Safe ATP-dependent DNase (Lucigen). In vitro cleavage reactions were performed with 250 ng of Plasmid-Safe-treated circularized DNA ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis and strand-specific library generation was carried out using the Script-Seq V2 Kit (Epicentre) and the FailSafe PCR enzyme mix ...
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bioRxiv - Genomics 2023Quote: ... with the following modifications as previously published37: Swabs were placed in tubes containing 350 ul Tissue and Cell Lysis solution (Lucigen), 1.5 mg/ml lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... then resuspend in 1mL resuspension buffer (20 mM Tris-HCl pH 7.5, 300 mM NaCl, 12500 U Ready-lyse (Epicentre technologies), and one tablet of EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Molecular Biology 2023Quote: ... Phage selections were amplified by adding them to 1.0 ml TG1 cells (Lucigen) grown to mid-log phase (OD ∼ 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...