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Citations for Lucigen :
451 - 500 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coli RNase I (10 U/µL, Epicentre) digestion occurred at room temperature for 45 minutes while lysate gently rotated or mixed ...
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bioRxiv - Neuroscience 2023Quote: ... the genomic DNA was extracted from single clones using QuickExtract solution (Lucigen, QE090500) and HDR was verified by locus specific PCR using DreamTaq DNA Polymerase (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... cloni 10g cells from Lucigen (Madison, WI) and E ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from 96-well plates of confluent clones using QuickExtract DNA Extraction Solution (Lucigen Corporation, Middleton, WI, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from triplicate co-cultures with the MasterPure complete DNA purification kit (Lucigen). The proportion of G ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Physiology 2023Quote: ... with RiboGuard RNase inhibitor (Lucigen, Cat # E0126-40D7) and oligo-dT primers (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli TG1 bacteria (Lucigen, Teddington, UK). Addition of the KM13 helper phage (kindly provided by Pierre Martineau ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 U/μL Ampligase (Lucigen A3210K) and 50 nM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... and Failsafe Buffer D (Epicentre). PCR products were run on a 1% agarose gel and imaged using a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: One swab each was stored in 350 μl Tissue and Cell lysis solution (Lucigen, #MTC096H, Middleton, WI) lysis buffer with 100 μl glass beads (0.1 diameter ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were incubated with Tobacco Acid Pyrophosphatase (Epicentre) and washed once with wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Standard PCRs were performed using StartWarm HS-PCR Mix (A&A Biotechnology) or EconoTaq PLUS2X Master Mix (Lucigen). PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium ...
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bioRxiv - Molecular Biology 2023Quote: ... and genomic DNA was extracted using QuickExtract solution (Lucigen, QE0950).
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bioRxiv - Microbiology 2023Quote: ... Version 2 (Lucigen, Middelton, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... subtilis CU1065 genomic DNA prepared using a DNA extraction kit (QuickExtract, Epicentre). PCR amplification was performed using primers YtqA_F and YtqB_R (Table S3 ...
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bioRxiv - Immunology 2023Quote: ... The pDNA for IVT were isolated from the E.coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen) using the Plasmid Maxi Kit (QIAGEN) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Lucigen), and harvested the plasmid DNA using the Qiaprep Spin miniprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Immunology 2023Quote: The pJAZZ-OK-based linear bacterial plasmids (Lucigen) with coding regions of every HAs were used as templates for mRNAs production ...
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bioRxiv - Biochemistry 2023Quote: ... cloni 10F ELITE Electrocompetent cells (Lucigen), and plated on two 140 mm Petri dishes containing LBkan-agar ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were selected and subcultured in THM to stationary phase and genomic DNA prepared using the Masterpure Gram-positive DNA purification kit (Lucigen, Middleton, WI). The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the bacterial pellets using the MasterPure™ Complete DNA and RNA purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µL BP reaction was electroporated into 25 µL Endura Electrocompetent cells (Lucigen, 60242) with the following conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Eluates were drop-dialyzed against 30 mL of water for 1 hour and transformed (Lucigen E ...
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bioRxiv - Genomics 2023Quote: ... and gel- purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described36 and libraries constructed using a different reverse indexing primer for each sample.
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bioRxiv - Molecular Biology 2023Quote: ... Purified cDNA was circularized using CircLigase II enzyme (Epicentre, CL9025K), then library PCR ...
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bioRxiv - Microbiology 2023Quote: ... coli (Lucigen) and induced according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA was collected using QuickExtract DNA extraction solution (Lucigen, #QE09050). The region surrounding the targeted site was PCR-amplified (F ...
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bioRxiv - Bioengineering 2023Quote: ... a PCR-amplified NLuc gene block was transcribed using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, ASF-3507) following manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and ribosomal RNAs were depleted using the Gram-negative bacteria RiboZero rRNA Removal Kit (Epicentre). Final eluates were used as input for strand-specific RNA-seq library construction using the NEBNext RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... each reaction mixtures were transformed into Endura™ electro-competent cells (Lucigen, CAT. 60242-1) and transformed bacteria were selected on LB + Ampicillin plates ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli DH5α or CopyCutter EPI400 (Lucigen) was used for cloning and grown in Luria-Bertani (LB ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Genetics 2023Quote: ... DNA were extracted using MasterPure yeast DNA purification kit (Epicentre, MPY80200). ChEC DNA was subjected to size selection using the Pippin Prep (SageScience ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen) were transformed with 2.5 µl of the eluate each ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were sorted in 5 μl of QuickExtract DNA Extraction Kit (Epicentre, USA) in 96-well format.
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bioRxiv - Neuroscience 2023Quote: 1 µg of BH RNAs was incubated with 1 U/µg RNase R (Lucigen RNR07250) at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was done by using the MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) to gain high quality and quantity DNA for metagenomic sequencing ...
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... and residual linear DNA was degraded by Plasmid-Safe ATP-dependent DNase (Lucigen). In vitro cleavage reactions were performed with 250 ng of Plasmid-Safe-treated circularized DNA ...