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Citations for Lucigen :
601 - 650 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was circularized by Circligase (Lucigen) and then re-linearized by BamHI ...
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bioRxiv - Genomics 2023Quote: ... 0.03μL UNG (Lucigen), 0.1μL EXO for internal control ...
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bioRxiv - Genomics 2023Quote: ... Circularization was performed with CircLigase I (Epicentre #CL4111K) instead of CircLigase II ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted with QuickExtract (Lucigen #QE09050) for amplicon sequencing.
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bioRxiv - Genomics 2023Quote: ... 1µl Fast-Link DNA ligase (Lucigen, cat. no. LK6201H), 3 µl 10× Fast link ligation buffer and 1mM ATP ...
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bioRxiv - Genomics 2023Quote: ... 0.2 µl of Fast-Link DNA Ligase (Lucigen, cat. no. LK6201H), 1.2 mM ATP ...
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bioRxiv - Genomics 2023Quote: ... Skin was sampled with an established protocol using pre-moistened Puritan foam swabs collected and stored in 100 µL Yeast Cell Lysis Buffer (Lucigen) buffer at -80° and shipped on dry ice ...
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions (Epicentre). Incubation was performed at 37°C for 90 min ...
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bioRxiv - Genomics 2023Quote: ... then were transformed into Endura Electrocompetent Cells (Lucigen, 602422) by electroporation (Gene Pulser Xcell Electroporation System ...
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen, QE09050), and correct PAX6 integration of the appropriate FT construct was confirmed by PCR ...
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bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... underwent a bead-based purification and circularised with CircLigase (Epicentre/Illumina, CL9021K) for 2 hrs ...
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Cancer Biology 2023Quote: ... the pBA439_UMI20 library was electroporated into 25 μl of Lucigen Endura ElectroCompetent Cells (Lucigen, 60242-2) using prechilled 1 mm electroporation cuvettes (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Extraction of template genomic DNA was performed by using QuickExtract (Epicentre QE0905T). 2 μl of genomic DNA extract solution was used to PCR amplify the genomic locus of interest (RFX2 ...
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bioRxiv - Immunology 2023Quote: ... and 200 U NxGen RNase inhibitor (Lucigen, #30281). Extracts were incubated on ice for 5 min prior to centrifugation at 17,000 g for 5 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... including treatment of the ligation reaction with PlasmidSafe DNase (Lucigen)49 ...
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bioRxiv - Genomics 2023Quote: ... Resulting colonies were picked and a portion of the cells lysed by QuickExtract (Lucigen, QE9050) and used to genotype by PCR with primers on either side of the insertion site (in most cases with one primer outside the homology arms ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted by using the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI) as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Free nucleic acids were digested using OmniCleave Endonuclease (Lucigen, USA) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using the MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, USA), including an additional bead-beating step using a FastPrep-24TM ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA was extracted using QuickExtract DNA Extraction Solution (QE09050; Epicentre) following the protocol recommended in the Alt-R genomic editing detection kit (1075931 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains Epi300 (Lucigen, Cat #: LGN-EC300110) and MC1061 (NCBI ...
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bioRxiv - Synthetic Biology 2023Quote: ... and it was then combined with EZ-Tn5 transposase (Lucigen) as described in the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was isolated using the MasterPure DNA Purification kit (Epicentre). We confirmed integrity of genomic DNA by gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal cell lines were isolated by single-cell sorting and characterized by genomic DNA extraction (Lucigen QuickExtract), PCR amplification of edited loci using Q5 polymerase (NEW ENGLAND BIOLABS ...
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bioRxiv - Genomics 2023Quote: ... coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Genomics 2023Quote: ... 20% End-It Enzyme Mix (Lucigen, E0025-D1), 18% Glycerol ...
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bioRxiv - Genomics 2023Quote: ... 150 μl ligation mix (1.5X Fast-link ligation buffer (Lucigen, SS000272-D5), 26.7 mM EGTA (bioWorld ...
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bioRxiv - Genomics 2023Quote: ... About 20,000 prepared barcode beads were resuspended in 40 μl enzyme buffer (1 U/μl Fast-Link DNA Ligase (Lucigen, E0077-2-D3), 20% End-It Enzyme Mix (Lucigen ...
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bioRxiv - Genomics 2023Quote: ... 1.2 mM ATP (Lucigen, SS000391-D3), 16% Glycerol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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bioRxiv - Cell Biology 2023Quote: Cells plated on glass coverslips were incubated with 5 µg/mL Brefeldin A (BFA) (Epicentre Biotechnologies, Madison, WI) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was randomly sheared through a syringe needle and was end-repaired and cloned into pWEB::TNC (Epicentre Technologies, Madison, WI), followed by packaging into MaxPlax lambda packaging extracts ...
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bioRxiv - Biochemistry 2023Quote: ... and the transcription was terminated with the addition of DNase I (Lucigen, ASF3507). RNA targets were purified with Monarch® RNA Cleanup Kit (T2040L ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA products were treated with Baseline-ZERO DNase I (Epicentre) and then purified again using the RNeasy Mini kit.
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was performed with ribosomal RNA (rRNA) depletion according to instructions from the manufacturer (Epicentre) to achieve greater coverage of mRNA and other long non-coding transcripts ...
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bioRxiv - Biochemistry 2023Quote: ... AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507, Lucigen) was used according to the manufacturer protocol with 4 μL PCR products per 20 μL reaction ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The end-repair reaction was done using the End-it DNA end-repair kit (Lucigen). P7 adapters were ligated to DNA fragments as described above ...
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bioRxiv - Immunology 2023Quote: ... The pDNA for IVT were isolated from the E.coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen) using the Plasmid Maxi Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µL BP reaction was electroporated into 25 µL Endura Electrocompetent cells (Lucigen, 60242) with the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... Version 2 (Lucigen, Middelton, WI, USA), according to the manufacturer’s instructions ...