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Citations for Lucigen :
601 - 650 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... followed by electroporation into Endura ElectroCompetent Cells (Lucigen, 60242-2) at ∼100x coverage ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ml of 37 °C Recovery Medium (Lucigen, 80026-1) was added and bacteria were grown in round-bottom tubes for 1 h at 37 °C while shaking at 180 r.p.m ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was later hydrolyzed by 250 mM NaOH and the cDNA was circularized using CircLigase II (Lucigen). The circularized cDNA was shipped to the Scripps La Jolla Genomics Core for final library preparation with Illumina sequencing adaptors and sequenced using single-end sequencing on the Illumina NextSeq 500 platform.
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bioRxiv - Plant Biology 2023Quote: ... according to the manufacturer’s instructions (Epicentre). Incubation was performed at 37°C for 90 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the pBA439_UMI20 library was electroporated into 25 μl of Lucigen Endura ElectroCompetent Cells (Lucigen, 60242-2) using prechilled 1 mm electroporation cuvettes (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... underwent a bead-based purification and circularised with CircLigase (Epicentre/Illumina, CL9021K) for 2 hrs ...
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA contamination was depleted using Baseline-Zero DNase (Lucigen/Epicentre, #DB0715K) according to manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Bioengineering 2023Quote: ... coli cells (Lucigen, catalog no. 60242-2) at 50–100 ng/ul ...
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bioRxiv - Immunology 2023Quote: ... coli SS320 host cells (Lucigen Corporation, Middleton, WI) were electroporated using 30-50 ng of each sub-library DNA in a 0.1 cm cuvette with the following settings ...
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bioRxiv - Immunology 2023Quote: ... coli ER2738 cells (Lucigen Corporation) for titer determination and overnight amplification ...
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bioRxiv - Developmental Biology 2023Quote: Tissue from resulting animals was lysed using QuickExtract DNA Extraction Solution (Epicentre) to release the genomic DNA ...
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bioRxiv - Bioengineering 2023Quote: ... TG1 electrocompetent cells were procured from Lucigen (Middleton, WI). M13KO7 helper phage particle ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µg of total RNA was mixed with 3 µl of diluted ERCC RNA spike-in mix and then digested for 1h at 30°C with 1 unit of Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in 1X Reaction Buffer A containing 10 units of SUPERase-In RNase inhibitor (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Evolutionary Biology 2023Quote: ... evonymella males was partially digested by Hind III and cloned into the pCC1BAC (Epicentre, Madison, WI, USA) vector ...
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bioRxiv - Cell Biology 2023Quote: ... TEV protease 96 or SUMO Express proteinase (Lucigen, 30801-2) was respectively incubated with GST-TEV-tagged G3BP1 proteins and His-SUMO-tagged N proteins at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from each clone using QuickExtract DNA solution (Lucigen) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans Fosmid Library (Source BioScience) and were amplified in bacteria using CopyControl Induction Solution (Lucigen, #CCIS125) and purified using a DNA midi prep kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cloni® 10G (MP21-5) was obtained from Lucigen (WI, USA). All strains used and constructed in this study are shown in Tab ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit ...
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bioRxiv - Genetics 2023Quote: ... and plasmid replication was induced by diluting 1:10 in LB + 12.5 µl/ml chloramphenicol and 1X CopyControl Induction Solution (Lucigen). The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Genetics 2023Quote: EPI300 bacterial cells (Lucigen) harboring the BAC clone CH322-154G8 (BACPAC Genomics ...
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bioRxiv - Microbiology 2023Quote: Skin and nasal cultures were obtained with Catch-all Collection Swabs (Epicentre) pre-moistened with Fastidious Broth (Remel) ...
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bioRxiv - Molecular Biology 2023Quote: ... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using QuickExtract (Epicentre) and PCR amplified across sgRNA sites (Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... for 30 seconds with nylon swabs (Epicentre, cat # QEC89100). Six dental swabs were collected per participant ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
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bioRxiv - Microbiology 2023Quote: ... and then treated with RNase R (Lucigen). After phenol/chloroform/isopropanol extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... which has a tetracycline resistance gene linked to the F+ gene was transformed via electroporation by incubating ER2738 electrocompetent cells (Lucigen) with ligated DNA in chilled electroporation cuvettes (0.1 cm gap ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from individual colonies was isolated using QuickExtract DNA extraction solution (Lucigen). Then ...
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bioRxiv - Genetics 2023Quote: Genomic DNA extraction from homogenized liver tissue was performed using the MasterPure Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Genetics 2023Quote: ... Fragments from 500 bp to 1000 bp were excised from an agarose gel and cloned into the pSMART LCKan plasmid (Lucigen) by transforming into competent cells ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen, QE09050), and correct PAX6 integration of the appropriate FT construct was confirmed by PCR ...
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bioRxiv - Genomics 2023Quote: ... DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre), and A-tailed with Klenow Fragment (3’-to-5’ exo- ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...