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Citations for Lucigen :
351 - 400 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... samples underwent rolling circle amplification using 1× Phi29 Buffer (Lucigen), 10% v/v Phi29 enzyme (Lucigen) ...
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bioRxiv - Genomics 2024Quote: ... 10% v/v Phi29 enzyme (Lucigen), 0.2 mg/ml BSA ...
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bioRxiv - Immunology 2024Quote: ... circularised with CircLigase II (Epicentre) and PCR amplified using Solexa P5/P7 primers ...
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bioRxiv - Genomics 2024Quote: ... 0.1 U/µL Ampligase (Lucigen #A3210K), and 10 mM dNTPs ...
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bioRxiv - Immunology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase™ 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesise RNA complementary DNA ...
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bioRxiv - Genomics 2024Quote: Cells were washed with 1X Ampligase buffer (Lucigen #A3210K) at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... before their resuspension in 650 µl BD Sample Buffer supplemented with a cocktail of Rnase inhibitors (1:1,000 SUPERase 20 U µl-1, AM2694 Thermo Fisher Scientific; NxGen Rnase Inhibitor 40 U µl-1, 30281-2 Lucigen). Cells were then loaded on a BD Rhapsody cartridge and their RNA was captured with the BD Rhapsody Express Single-Cell Analysis System following the manufacturer’s instructions (BD Biosciences ...
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bioRxiv - Biochemistry 2024Quote: ... and RecJ exonuclease (Lucigen/Epicenter), and the linker-ligated footprints were then purified via a Oligo Clean and Concentrator kit (Zymo) ...
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bioRxiv - Biochemistry 2024Quote: ... Extracted cDNA was then circularized using CircLigase II (Lucigen). Circular DNA was quantified via qPCR ...
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bioRxiv - Systems Biology 2024Quote: ... X11 or X8 plasmid library was used to transform 25 µL of electrocompetent MC1061 F- E.coli (Lucigen ≥4 × 1010 cfu/µg) in a 1 mm electroporation cuvette and micropulser (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... The same plasmid (section 0.1) used for the experiments was transformed into BL21(DE3) (Lucigen) cells and plated ...
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bioRxiv - Synthetic Biology 2024Quote: ... Two tubes of 50uL Endura Electrocompetent Cells (Lucigen, Catalog #60242-2) were transformed with 2uL per tube of column-purified libraries as per manufacturer instruction ...
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bioRxiv - Synthetic Biology 2024Quote: ... The QuickExtract DNA Extraction Kit (Lucigen) extracted nucleic acids from all infected samples ...
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bioRxiv - Bioengineering 2024Quote: ... 20 U RNase R and 10× RNase R buffer (Epicentre) was added according to manufacturer’s protocols and was heated to 37 for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... coli cells (Lucigen) for propagation ...
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bioRxiv - Bioengineering 2024Quote: ... The Golden Gate reaction was purified via DNA precipitation with isopropanol and 500 ng was transformed into Endura ElectroCompetent Cells (Lucigen, catalog no. 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase™ 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesise RNA complementary DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted from individual samples using 40 µL QuickExtract DNA extraction solution (Lucigen QE0905T). Embryos were incubated for 15 min at 25 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We purified PCR products by manually excising them from 2% low-melting-point agarose gels and incubating them at 45 °C for 3 h with GELase (Epicentre).
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bioRxiv - Genetics 2024Quote: ... tail snips were collected and lysed using Quick Extract buffer (Lucigen Cat no.QE09050) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... During this process individual embryos were collected at 6 and 24 hpf in QuickExtract™ DNA Extraction Solution (Lucigen) for Indel mutations genotyping using MiSeq sequencing.
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bioRxiv - Genomics 2024Quote: ... and cellular debris precipitated using steps and reagents from the MasterPure Yeast DNA Purification Kit (Lucigen). DNA was finally purified using the Monarch Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... Incorporation of the fluorophore into dsDNA was done through a primer extension reaction using EconoTaq polymerase (Lucigen, 30035–2) and purified using the QIAquick PCR purification kit (Qiagen 28106) ...
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bioRxiv - Genomics 2024Quote: ... using the Shotgun PCR Free Library Kit (Lucigen) and the NovaSeq™ 6000 Sequencing device (Illumina® ...
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bioRxiv - Genomics 2024Quote: ... reactions were supplemented with 10 µL of RNase H mix (10 U Hybridase Thermostable RNase H (Lucigen; H39500), 20 mM MgCl2 ...
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bioRxiv - Systems Biology 2024Quote: ... cDNA libraries were constructed by using the TotalScript RNA-Seq kit from Epicentre (discontinued), a transposon-based method of tagmentation (simultaneous tagging and fragmentation ...
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bioRxiv - Synthetic Biology 2024Quote: 25 μL aliquots of Endura DUO electrocompetent cells (Lucigen #60242-2) were thawed on ice and mixed with 2 μL of the purified Golden Gate product ...
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bioRxiv - Genomics 2024Quote: ... 4 uL of the elutant per tube were transformed into 50 mL Endura electrocompetent cells (Lucigen, catalog no. 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 2 μL of each Golden Gate reaction were transformed into 15 μL Endura cells (Lucigen, catalog no. 60242-2) and single colonies were recovered from overnight incubation on Carbenicillin agar plates ...
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bioRxiv - Genomics 2024Quote: Cloned libraries were amplified by electroporation into Endura electrocompetent cells (Lucigen). Using dilution plates for colony counting ...
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bioRxiv - Genetics 2024Quote: ... Fragments containing the biotinylated adaptor were further purified with Dynabeads M-280 streptavidin (Thermo) and DNA ends were repaired using the End-it DNA End-repair kit (Lucigen). P7 adaptors were ligated to DNA fragments ...
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bioRxiv - Genetics 2024Quote: ... 50-100,000 cells were pelleted and resuspended in 50µL of Quick Extract (QE; Lucigen; QE09050) to extract gDNA ...
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bioRxiv - Genetics 2024Quote: ... Cells (50-100,000 cells) were pelleted and resuspended in 50µL of Quick extract (QE; Lucigen; QE09050) to extract gDNA ...
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bioRxiv - Genetics 2024Quote: ... Clones that displayed one or two fluorescent spots per nucleus were further split in a 6-well plate and genomic DNA (gDNA) was extracted (Lucigen QE0905T). PCR (Promega M7801 ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) (prewarmed to 65°C ...
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bioRxiv - Bioengineering 2024Quote: Cells were lysed with QuickExtract (QE0905T, Lucigen) and gDNA was purified using SPRI magnetic bead cleanup ...
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bioRxiv - Biochemistry 2024Quote: ... Single clones were expanded to 6-well plates after ∼ 2 weeks of growth on 24-well plates and genomic DNA was extracted using Quick Extract DNA extraction solution (Lucigen). Primers flanking the expected deletion region (VB243 and VB244 ...
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Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanningbioRxiv - Biochemistry 2024Quote: ... coli SS320 cells (Lucigen) pre-infected with M13KO7 helper phages (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA extracts from GBM patients’ tissues were treated with RNase R according to the manufacturer’s protocol (Lucigen). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... and Plasmid-Safe ATP-dependent DNase (Lucigen) to enzymatically degrade remaining linear DNA molecules ...
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bioRxiv - Biochemistry 2024Quote: ... the EZ-Tn5
transposon kit (Epicentre) and the E54K/M56A/L372P Tn5 hyperactive variant transposase were used for transposon mutagenesis ... -
bioRxiv - Biophysics 2024Quote: Each PCR-purified pool was transformed into bacteria by combining 2 µL of the pool with 25 µL of electrocompetent cells (Lucigen). The DNA/cell mixture was added to a chilled cuvette and electroporated using a Gene Pulser Xcell (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... coli cells (Lucigen) for protein expression.
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bioRxiv - Cancer Biology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesize RNA complementary DNA ...
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bioRxiv - Cell Biology 2024Quote: ChIP and input DNA from both U2OS and hFOB 1.19 were prepared for sequencing as directed by Lucigen End-It DNA repair Kit ...
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bioRxiv - Cell Biology 2024Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Cell Biology 2024Quote: End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 250C for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with 1 U/μl NxGen Phi29 DNA polymerase (Lucigen, cat# 302210-2), 250 μM dNTPs ...
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bioRxiv - Cell Biology 2024Quote: ... cloni 5-α (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Cell Biology 2024Quote: ... 50k TdTomato+ cells from 4 brains were lysed with QuickExtract (Lucigen) according to manufacturer’s instructions ...