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Citations for Lucigen :
701 - 750 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and 0.2U/uL RNAse inhibitor (Lucigen). The tissue was then triturated 15 times through a fire-polished Pasteur pipette and then passed through a 40 uM filter to remove cell debris and large chunks ...
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bioRxiv - Neuroscience 2023Quote: ... We also propagated SCN1A into CopyCutter EPI400 cells (formerly Lucigen, now BioSearch Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... into pSMART-HC-Kan (Lucigen) vectors along with upstream CMV promoters and downstream IRES2-SYFP2 or IRES2-mScarlet transfection reporters for expression in HEK-293 cells ...
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bioRxiv - Neuroscience 2023Quote: ... To minimize rearrangement of full length SCN1A we used pSMART-HC-Kan backbone (Lucigen) which helps minimize backbone-driven transcription in bacteria ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was maintained in 10G elite Escherichia coli bacteria (Lucigen).
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bioRxiv - Molecular Biology 2023Quote: ... Standard PCRs were performed using StartWarm HS-PCR Mix (A&A Biotechnology) or EconoTaq PLUS2X Master Mix (Lucigen). PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium ...
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bioRxiv - Molecular Biology 2023Quote: ... and genomic DNA was extracted using QuickExtract solution (Lucigen, QE0950).
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bioRxiv - Cell Biology 2023Quote: ... and genomic DNA was collected using QuickExtract DNA extraction solution (Lucigen, #QE09050). The region surrounding the targeted site was PCR-amplified (F ...
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bioRxiv - Microbiology 2023Quote: ... Total RNAs (450 ng) were treated with DNase (Baseline-ZERO DNase, Epicentre) and ribosomal RNA was removed using the Illumina Ribo-Zero Magnetic Kit (Bacteria) ...
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bioRxiv - Microbiology 2023Quote: ... the cell resuspensions were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: Total RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized using the PrimeScript RT Master Mix (Takara).
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bioRxiv - Microbiology 2023Quote: ... and purified DNA-free RNA samples were subjected to ribosomal depletion with Ribo-Zero™ Magnetic Kits (Epicentre®, Singapore), all according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Microbiology 2023Quote: ... and Epicenter Masterpure Yeast DNA Purification buffer (Epicentre, Madison, WI). Cells were bead beat for 5 cycles (1 minute on ...
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bioRxiv - Microbiology 2023Quote: ... The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4) by blunt-end ligation enzyme (Epicentre FastLinkTM kit) and ligation products in the range of 4-10 kb were extracted from a 0.7% agarose gel.
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using Lucigen MasterPure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). DNA concentration was measured by bio-spectrophotometer absorbance readings and diluted to desired target concentrations.
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bioRxiv - Microbiology 2023Quote: ... 50 U/μL Ready-Lyse Lysozyme Solution (Lucigen, WI, USA), 2 U/mL Zymolyase (Zymo Research Corporation ...
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bioRxiv - Microbiology 2023Quote: ... Triphosphate in 5’ were removed using a RNA 5’ polyphosphatase (Euromedex Lucigen) and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman) ...
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bioRxiv - Neuroscience 2023Quote: DNA was prepared by either QuickExtract (Lucigen) from iPSCs or Qiagen DNeasy columns (MSNs and U2OS ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA extraction was performed using QuickExtract™ DNA Extraction Solution (Lucigen), and the genomic locus of interest was amplified using AmpliTaq Gold® 360 Master Mix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL DNA QuickExtract solution (Lucigen QE09050) was added ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2023Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI). Remaining steps followed the recommended PureLink Genomic DNA Mini Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Molecular Biology 2023Quote: DNA from GFP-positive cells was isolated using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... from ClearColi (Lucigen) were purified using a Ni-affinity column ...
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bioRxiv - Molecular Biology 2023Quote: Remaining linear DNA molecules were digested with Plasmid-Safe ATP-dependent exonuclease (Epicentre) continuously at 37°C for 120 h ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL TypeOne Restriction Inhibitor (Lucigen, USA) and 0.4 µL transposome ...
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bioRxiv - Microbiology 2023Quote: ... Transposomes were prepared using 100 ng transposon DNA and EZ-Tn5 transposase (Lucigen, USA) according to the suppliers specifications ...
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bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Immunology 2023Quote: ... Immediately after electroporation 1 ml of prewarmed recovery medium (Lucigen, #800261 was added to the bacteria and the cells were incubated for 1 hour at 37 °C shaking at 250 rpm in a bacterial incubator ...
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bioRxiv - Genomics 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
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bioRxiv - Genomics 2023Quote: ... Monophosphorylated RNAs were selectively degraded by 1 hour incubation with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). Subsequently ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain EPI300 (Epicentre, USA) under chloramphenicol selection for attB libraries ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
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bioRxiv - Microbiology 2023Quote: ... coli C43 (DE3) electro-competent cells (Lucigen) modified to incorporate a pspA gene knockout60 ...
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bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Microbiology 2023Quote: Tn-5 transposon insertion library was build based on EZ-Tn5™
Tnp transposome system (Lucigen, WI, USA). Competent C ... -
bioRxiv - Molecular Biology 2023Quote: ATAC-see in 293T cells was done with the EZ-Tn5™ Transposase (Lucigen TNP9211), following the published protocol36 ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of cells were mixed with EZ-Tn5TM
transposome (Lucigen) and incubated on ice for 30 min ... -
bioRxiv - Microbiology 2023Quote: Phage DNA was isolated using the MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA) with modifications ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Molecular Biology 2023Quote: Digestion of cellular RNA preperations with Terminator™5’Phosphate-Dependent Exonuclease (Epicentre) was perforemd to remove the abundant rRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was circularized with CircLigase (Epicentre), pooled and then amplified with PCR primers with Illumina sequencing adapters as desribed previously 55,56 ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA was extracted with QuickExtract (Lucigen) and PCR was performed using Q5® High-Fidelity DNA Polymerase according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... RNase inhibitor (Lucigen) was added at 0.4 U/µl throughout the incubation until the rolling cycle amplification (RCA ...