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Citations for Lucigen :
551 - 600 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... evonymella males was partially digested by Hind III and cloned into the pCC1BAC (Epicentre, Madison, WI, USA) vector ...
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bioRxiv - Cell Biology 2023Quote: ... TEV protease 96 or SUMO Express proteinase (Lucigen, 30801-2) was respectively incubated with GST-TEV-tagged G3BP1 proteins and His-SUMO-tagged N proteins at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans Fosmid Library (Source BioScience) and were amplified in bacteria using CopyControl Induction Solution (Lucigen, #CCIS125) and purified using a DNA midi prep kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from each clone using QuickExtract DNA solution (Lucigen) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Microbiology 2023Quote: Skin and nasal cultures were obtained with Catch-all Collection Swabs (Epicentre) pre-moistened with Fastidious Broth (Remel) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from individual colonies was isolated using QuickExtract DNA extraction solution (Lucigen). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... which has a tetracycline resistance gene linked to the F+ gene was transformed via electroporation by incubating ER2738 electrocompetent cells (Lucigen) with ligated DNA in chilled electroporation cuvettes (0.1 cm gap ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using QuickExtract (Epicentre) and PCR amplified across sgRNA sites (Table S1) ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... for 30 seconds with nylon swabs (Epicentre, cat # QEC89100). Six dental swabs were collected per participant ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
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bioRxiv - Microbiology 2023Quote: ... and then treated with RNase R (Lucigen). After phenol/chloroform/isopropanol extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre), and A-tailed with Klenow Fragment (3’-to-5’ exo- ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA contamination was depleted using Baseline-Zero DNase (Lucigen/Epicentre, #DB0715K) according to manufacturer’s instructions ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µg of total RNA was mixed with 3 µl of diluted ERCC RNA spike-in mix and then digested for 1h at 30°C with 1 unit of Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in 1X Reaction Buffer A containing 10 units of SUPERase-In RNase inhibitor (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Genomics 2023Quote: ... 200µL of the cell suspension was washed twice in PBS and genomic DNA was extracted with Quickextract solution (Lucigen, QE0905T) according to the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2023Quote: ... the QuickExtract™ DNA Extraction Solution method (Lucigen), or a salt extraction method (Aljanabi & Martinez ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
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bioRxiv - Bioengineering 2023Quote: ... TG1 electrocompetent cells were procured from Lucigen (Middleton, WI). M13KO7 helper phage particle ...
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bioRxiv - Bioengineering 2023Quote: ... coli cells (Lucigen, catalog no. 60242-2) at 50–100 ng/ul ...
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bioRxiv - Immunology 2023Quote: ... coli SS320 host cells (Lucigen Corporation, Middleton, WI) were electroporated using 30-50 ng of each sub-library DNA in a 0.1 cm cuvette with the following settings ...
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bioRxiv - Immunology 2023Quote: ... coli ER2738 cells (Lucigen Corporation) for titer determination and overnight amplification ...
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bioRxiv - Developmental Biology 2023Quote: Tissue from resulting animals was lysed using QuickExtract DNA Extraction Solution (Epicentre) to release the genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were sequentially treated with Terminator 5‘-Phosphate-Dependent Exonuclease (Lucigen), Quick CIP (calf-intestine alkaline phosphatase ...
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bioRxiv - Microbiology 2023Quote: ... Linear single-stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were lysed using QuickExtract DNA Extraction Solution (Lucigen) following the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: The plasmid pETite (Lucigen) containing the recombinant VHL (M54 – D213 ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37 °C for 5 min.The successful linearization of ecDNA was verified by verifying its sensitivity to exonucleaseATP-dependent DNase (Lucigen). After treatment with exonuclease ...
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bioRxiv - Cancer Biology 2023Quote: ... the genomic DNA was treated with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... linear contaminant DNAs within the crude circular DNA are digested with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The ecDNA samples were extracted and the sensitivity to the exonuclease ATP-dependent DNase (Lucigen) was verified in order to determine the circular DNA samples obtained ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli cells (Lucigen Corp.) using electroporation ...
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bioRxiv - Molecular Biology 2023Quote: Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina) was used to remove ribosomal RNAs and to enrich mRNAs ...
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bioRxiv - Molecular Biology 2023Quote: Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen) was used to enrich the 5′ ends of the transcripts ...