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Citations for Lucigen :
301 - 350 of 2386 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: EPI300 bacterial cells (Lucigen) harboring the BAC clone CH322-154G8 (BACPAC Genomics ...
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bioRxiv - Microbiology 2023Quote: Skin and nasal cultures were obtained with Catch-all Collection Swabs (Epicentre) pre-moistened with Fastidious Broth (Remel) ...
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bioRxiv - Molecular Biology 2023Quote: ... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using QuickExtract (Epicentre) and PCR amplified across sgRNA sites (Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... for 30 seconds with nylon swabs (Epicentre, cat # QEC89100). Six dental swabs were collected per participant ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
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bioRxiv - Microbiology 2023Quote: ... and then treated with RNase R (Lucigen). After phenol/chloroform/isopropanol extraction ...
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bioRxiv - Microbiology 2023Quote: ... After an initial step of lysis using 0.3 μl Ready-Lyse Lysozyme (Epicentre, Madison, WI, USA) at 37°C with 500 rpm shaking for 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... which has a tetracycline resistance gene linked to the F+ gene was transformed via electroporation by incubating ER2738 electrocompetent cells (Lucigen) with ligated DNA in chilled electroporation cuvettes (0.1 cm gap ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from individual colonies was isolated using QuickExtract DNA extraction solution (Lucigen). Then ...
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bioRxiv - Genetics 2023Quote: Genomic DNA extraction from homogenized liver tissue was performed using the MasterPure Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Genetics 2023Quote: ... Fragments from 500 bp to 1000 bp were excised from an agarose gel and cloned into the pSMART LCKan plasmid (Lucigen) by transforming into competent cells ...
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bioRxiv - Immunology 2023Quote: ... coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8 ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Systems Biology 2023Quote: ... and self-ligated using Fast-link ligase (LK0750H; Lucigen). Duplets of 800bp-1000bp were excised from agarose gel ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen, QE09050), and correct PAX6 integration of the appropriate FT construct was confirmed by PCR ...
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bioRxiv - Genomics 2023Quote: ... DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre), and A-tailed with Klenow Fragment (3’-to-5’ exo- ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Microbiology 2023Quote: ... Linear single-stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Genomics 2023Quote: ... including treatment of the ligation reaction with PlasmidSafe DNase (Lucigen)49 ...
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bioRxiv - Genomics 2023Quote: ... Resulting colonies were picked and a portion of the cells lysed by QuickExtract (Lucigen, QE9050) and used to genotype by PCR with primers on either side of the insertion site (in most cases with one primer outside the homology arms ...
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bioRxiv - Genomics 2023Quote: ... then were transformed into Endura Electrocompetent Cells (Lucigen, 602422) by electroporation (Gene Pulser Xcell Electroporation System ...
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bioRxiv - Cell Biology 2023Quote: Cells plated on glass coverslips were incubated with 5 µg/mL Brefeldin A (BFA) (Epicentre Biotechnologies, Madison, WI) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Ampligase DNA Ligase and 10X Reaction Buffer were purchased from Lucigen (A3202K). 2X GoTaq G2 Hot Start Colorless Master Mix was purchased from Promega (9IM743) ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen). First strand cDNA synthesis was performed using the EasyScript Plus cDNA Synthesis Kit (Lamda Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... the library was electroporated into Escherichia coli cells (E. cloni 10G Elite; Lucigen, USA), yielding ≈ 107 colonies after overnight incubation on agar plates ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was isolated from yeast and contaminating DNA was depleted using the MasterPure Yeast RNA Purification Kit (Epicentre, Madison, WI) protocol with minor changes as previously described (Carrocci et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were incubated for 72h and harvested with Quick Extract (Lucigen). Genomic DNA was amplified using genomic region-specific primers (Supplemental) ...
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Lucigen, #60242) and 3×106 colonies resulting from the transformation were pooled together to generate a barcode plasmid DNA ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
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bioRxiv - Genetics 2023Quote: ... at least 30 5-FOA resistant clones were grown in YEPD for DNA extraction by MasterPure™ Yeast DNA Purification Kit (Lucigen). The relative location of each chromosome truncation event was determined using multiplex PCR with primers that anneal centromere or telomere proximal to the SiRTA (File S1) ...
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bioRxiv - Genetics 2023Quote: ... Cells were harvested using Quick Extract (Lucigen) per the manufacturer’s instructions beginning at 2 days after transfection until 6 days after transfection ...
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bioRxiv - Genetics 2023Quote: ... Cell pellets were resuspended in 50 µL of Quick Extract (Lucigen), and genomic DNA was prepared per the manufacturer’s instructions.
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
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bioRxiv - Cancer Biology 2023Quote: RNA-sequencing (RNA-seq) was performed using either ScriptSeq (EpiCentre) library preparation or using the KAPA RNA HyperPrep Kit (RiboErase HMR ...
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bioRxiv - Cancer Biology 2023Quote: ... The final product was transformed into electrocompetent cells (Lucigen), incubated overnight in LB broth at 37 °C ...