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Citations for Lucigen :
301 - 350 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: 2 μg of total RNA were treated with 4U of RNase R (Lucigen) at 37°C for 5 or 10 minutes in case of downregulated and at 37°C for 30 minutes for upregulated circRNAs followed by 20 minutes at 65°C ...
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bioRxiv - Synthetic Biology 2024Quote: Genomic DNA from overnight saturated cultures of isogenic bacterial clones was prepared using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) according to the manufacturer’s guidelines and sequenced at SeqCenter (Pittsburgh ...
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bioRxiv - Synthetic Biology 2024Quote: ... plasmid and contains an additional oriV origin-of-replication for potential conditional amplification in EPI300 cells (Epicentre). Following yeast assembly ...
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bioRxiv - Systems Biology 2024Quote: Plasmid DNA was extracted both for MNase-activated cells and non-activated cells using the MasterPure Yeast DNA Purification Kit (Lucigen Corporation, MPY80200), with some modifications ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X RNase H buffer (Epicentre) and 6 µL of nuclease-free water were added and the samples were incubated at 45°C for 30 min ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of thermostable RNase H (10 units; Epicentre), 2 µL of 10X RNase H buffer (Epicentre ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was gel purified and circularized with Circ Ligase (Lucigen). Libraries were PCR amplified using forward primer oBZ287 (NINI2 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then end-repaired using the End-It kit (Lucigen), A-tailed with Klenow 3’-5’ exo-(NEB) ...
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bioRxiv - Molecular Biology 2024Quote: ... or QuickExtract DNA extraction solution (Lucigen, #QE0905T) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cells were resuspended in 50 µl of QuickExtract DNA Extraction Solution (Lucigen, Catalog #QE09050). Genomic DNA was extracted by treating the cells to the following protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect genomic DNA (gDNA) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of Hybridase Thermostable RNase H (Lucigen) was added to each tube and incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was isolated (QuickExtract, Cat #QE0905T, Lucigen) and the gRNA-targeted site was amplified by PCR (primers listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... 20,000 U of Ready-Lyse lysozyme (Epicentre, Madison, WI, USA), and 40 U of SUPERase-In RNase inhibitor for 5 min at room temperature to extract total RNA ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 500mL TES (10mM Tris-HCl, pH 7.5, 1mM EDTA, 100mM NaCl) and incubated with 35,000U of Ready-Lyse (Lucigen) for 10min at room temperature ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Disruption of both alleles of the target locus and integration of the RT were confirmed by PCR after DNA extraction (MasterPure Yeast DNA Purification Kit, Lucigen). The SAT1 gene was removed with the flipper system ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA extracted (QuickExtract™ DNA Extraction Solution, Lucigen, cat. QE09050) and the remainder restimulated with fresh medium and cultured for an additional 14 days (Jones et al. ...
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bioRxiv - Microbiology 2024Quote: ... and rRNA was removed from 1 mg of total RNA with Ribo-Zero Magnetic Gold Kit (Epicentre Biotechnologies, Madison, WI, USA). To construct the RNA-Seq library ...
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bioRxiv - Microbiology 2024Quote: ... The parasite gDNA was extracted from the infected mosquito midguts 14 days post-infection (Barcode output) by boiling the midguts in Quick Extract DNA Extraction Solution (Epicentre). PlasmoGEM barcodes were amplified by PCR in using the extracted DNA samples and the primers 91_Illumina and 97_Illumina (Table S6) ...
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bioRxiv - Microbiology 2024Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Microbiology 2024Quote: ... was added to 0.3% final concentration and Plasmid-Safe ATP-dependent DNase (Epicentre E3105K) was added to 20 U/ml final concentration to digest any free unpackaged DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... cloni classic electrocompetent cells (Lucigen, 60117-1). Plasmid DNA was isolated and representation of the pool was verified by Illumina sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... a fraction of the cells was used for genomic DNA extraction using QuickExtract DNA Solution (Lucigen). PCR from genomic DNA was performed using KAPA2G Fast Hotstart Ready Mix PCR Kit (Kapa Biosystems) ...
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bioRxiv - Microbiology 2024Quote: Transposon mutagenesis was performed by electroporation using the EZ-Tn5
Tnp Transposome kit (Lucigen). Briefly ... -
bioRxiv - Biochemistry 2024Quote: ... 1U/µl RiboGuard RNase Inhibitor (Lucigen; RG90925), 0.1 U/µl 5’-Phosphate-Dependent Exonuclease ...
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bioRxiv - Cell Biology 2024Quote: ... 25 % of the suspension was used for gDNA extraction (QuickExtract, Lucigen, QE09050) and validation of gene editing by PCR ...
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bioRxiv - Physiology 2024Quote: ... genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen). The genomic region targeted with the guide RNA was amplified by PCR (Forward primer sequence ...
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bioRxiv - Microbiology 2024Quote: Microbial DNA extraction was performed following the MasterPure Complete DNA and RNA Purification Kit (Epicentre, Southampton, Hampshire, UK) and Pathogen Lysis Tubes (QIAGEN ...
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bioRxiv - Neuroscience 2024Quote: ... Ribo-Zero-selected (Epicentre, Madison, WI), DNase-treated (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... and genomic DNA was extracted using QuickExtract (Lucigen Cat # QE09050), and a PCR over the region of interest was then conducted ...
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bioRxiv - Pathology 2024Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2× Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follows ...
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bioRxiv - Systems Biology 2024Quote: ... We performed nine electroporations of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2024Quote: The prepared Ds-inserted DNA pools (around 1.5 to 2.5 pmol) were subjected to self-ligation (10μl) by 50 Units of Circligase II (Lucigen) in the circligation buffer supplemented with 2.5mM MnCl2 and 1M Betain for 16 h at 60°C ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity ...
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bioRxiv - Biophysics 2024Quote: Recombinant LifA containing a C-terminal His-tag in the pRham Vector (Lucigen) [9] was overexpressed in E ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was treated with the Ribo-Zero Gold kit (Human/Mouse/Rat, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribo-Zero treated RNA was used to create multiplexed RNA-seq libraries using the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and C41(DE3) (Lucigen) respectively.
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was isolated using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... for sorting and then genomic DNA was extracted using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre), adhering to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cells with QuickExtract (Lucigen #QE0905), column-purified (Zymo Research #C1102-50) ...
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bioRxiv - Microbiology 2024Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI) and vortexed for 10s ...
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2024Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI) and vortexed for 10s ...
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2024Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... 3 mL of saturated culture was pelleted and used according to manufacturer instructions following the MasterPure Complete DNA and RNA Purification Kit (Lucigen MC85200). Isolated genomic DNA was quantified using a NanoDrop and used as input for the Illumina DNA Prep Tagmentation Kit (formerly Nextera DNA Flex ...
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bioRxiv - Plant Biology 2024Quote: ... and Ribo-Zero rRNA Removal kit (Epicentre), respectively ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from iPSCs and NPCs using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The NxSeq AmpFREE Low DNA Library Kit (Lucigen - CA10837-144) was then used ...