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Citations for Lucigen :
301 - 350 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... cloni 10G supreme (#60081-1; Lucigen).
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bioRxiv - Systems Biology 2024Quote: ... This pool of elements was end-repaired using End-It DNA End-Repair Kit (#ER0720; Epicentre) and self-ligated using Fast-link ligase (LK0750H ...
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bioRxiv - Systems Biology 2024Quote: ... cloni 10G supreme (#60081-1; Lucigen). To estimate the approximate complexity of the resulting libraries ...
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bioRxiv - Microbiology 2024Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA from isolates exhibiting uracil auxotrophy was purified using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The promoter ...
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bioRxiv - Neuroscience 2024Quote: ... Offspring genotypes were confirmed by PCR (Lucigen EconoTaq Plus GREEN 2X) and using two-photon imaging to observe expression of the reporter protein (GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 min at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... 25 % of the suspension was used for gDNA extraction (QuickExtract, Lucigen, QE09050) and validation of gene editing by PCR ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted with QuickExtract DNA extraction solution (EpiCentre) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (1 μg) from untreated HPAECs was digested by 2U of RNase R enzyme (Lucigen, Middleton, WI) in 10 μL reactions containing 1x digestion buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 U Baseline-ZERO DNase (Lucigen) in Baseline-ZERO DNase Buffer for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR and HeLa S3 RNAs (1 μg) were incubated with 20 U exonuclease I (Lucigen) and 2 U Baseline-ZERO DNase (Lucigen ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from 10,000 – 50,000 target cells by resuspending them in a QuickExtract DNA Extraction Solution (LGN-QE09050, Lucigen). PCR amplification of the targeted junction from the wild-type and tagged cells was performed using primers designed to anneal outside of the homology arms of the specific gene ...
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bioRxiv - Biophysics 2024Quote: Recombinant LifA containing a C-terminal His-tag in the pRham Vector (Lucigen) [9] was overexpressed in E ...
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bioRxiv - Cell Biology 2024Quote: ... the Brunello library was amplified in Endura electrocompetent cells (Lucigen) and subjected to Next-Generation Sequencing (NGS ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was treated with the Ribo-Zero Gold kit (Human/Mouse/Rat, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribo-Zero treated RNA was used to create multiplexed RNA-seq libraries using the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... DNA was isolated using QuickExtract DNA Solution (Lucigen) and amplicons were generated using 15 cycles of PCR to introduce Illumina sequencing primer binding sites and 0-8 staggered bases to ensure library diversity ...
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bioRxiv - Molecular Biology 2024Quote: ... The size-selected RNAs or synthetic versions of FLEXIs were subjected to exonuclease treatment (1 U Terminator exonuclease (Lucigen), 20 U RNase R (Abcam) ...
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bioRxiv - Physiology 2024Quote: ... For each primer pair PCR was performed for the control and corresponding RNAi or overexpression samples using EconoTaq PLUS Green (Lucigen) on an iCycler Thermocycler (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... genomic DNA was isolated with the QuickExtract DNA Extraction Solution (Lucigen). The genomic region targeted with the guide RNA was amplified by PCR (Forward primer sequence ...
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bioRxiv - Pathology 2024Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2× Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was isolated using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... for sorting and then genomic DNA was extracted using the QuickExtract™ DNA Extraction Solution (QE09050, Epicentre), adhering to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The Circligase II kit (CL9021K) was from Lucigen; the RNA Clean and Concentrator kit (R1019 ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using the MasterPure Complete DNA Purification kit (Lucigen). Sequencing libraries were generated through two sequential rounds of PCR using Herculase II Fusion DNA polymerase (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA of sorted cells was extracted with QuickExtractTM DNA Extraction Solution (Lucigen) and amplified by PCR using Phusion High-Fidelity DNA Polymerase (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted with QuickExtract™ DNA Extraction Solution (Lucigen) and the region of interest was amplified by PCR using Phusion High Fidelity DNA Polymerase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... pSMART BAC vector (Lucigen) was first linearized by NotI digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting mixture was transformed into BAC-optimized replicator v2.0 electrocompetent cells (Lucigen), yielding pSMART BAC F (1,5) ...
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bioRxiv - Molecular Biology 2024Quote: ... between the EcoRI and BamHI sites of pCC1BAC (Epicentre Biotechnologies) (Wang et al ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase H (10 U; Lucigen, H39500) was added and the mixture incubated at 45°C for 30 min in 20 µl containing 50 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40-60 μg of RNA from the lysate were treated with RNAse I (Lucigen, Cat. #N6901K) for 50 minutes at room temperature while rotating ...
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bioRxiv - Microbiology 2024Quote: rRNA was removed from 35.71 ng of total RNA using the Ribo-Zero(TM) rRNA Removal Kit (Epicentre). The rRNA depleted RNA was fragmented and reverse transcribed using random hexamers and SSII (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... ReadyLyse (LUCIGEN) and lysozyme were added after bead-beating ...
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bioRxiv - Microbiology 2024Quote: ... MPC protein precipitation reagent (Lucigen) was added ...
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bioRxiv - Microbiology 2024Quote: The transposon sequence was constructed by stitch PCR using the commercially available EZ-Tn5 KanR transposon (Lucigen), and a previously published CmR mini-Tn5 transposon45 as a template and cloned into the pUC19 vector ...
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bioRxiv - Genetics 2024Quote: ... DNA was end-repaired using the End-IT DNA End-Repair Kit (Lucigen, ER81050) and subsequently A-tailed using Klenow Fragment (3’ -> 5’ Exo- ...
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bioRxiv - Genetics 2024Quote: ... End-repaired using End-IT DNA End-Repair kit (Lucigen, ER81050), subsequently bead purified and digested with EcoRI-HF (NEB ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from iPSCs and NPCs using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The NxSeq AmpFREE Low DNA Library Kit (Lucigen - CA10837-144) was then used ...
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bioRxiv - Microbiology 2024Quote: ... 1 × protease inhibitors) and lysed using 120 kU Ready-Lyse lysozyme (Lucigen). Native elongating complexes were released from chromosome by treatment with 6 U Turbo DNase (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and subjected to treatment with 5’-phosphate-dependent exonuclease (Epicentre) as required.
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bioRxiv - Microbiology 2024Quote: ... utilizing 40 U of ssDNA Ligase and 50 µM ATP (Lucigen).
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bioRxiv - Microbiology 2024Quote: ... and subsequently treated with or without 2 U 5’-phosphate-dependent exonuclease (Epicentre) at 30°C for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 mM MgCl2 and ribodepleted using Hybridase thermostable RNaseH (Epicentre, H39500). Afterward ...
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA was extracted using QuickExtract (Lucigen) as previously described (17) ...
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bioRxiv - Bioengineering 2024Quote: ... and genomic DNA was extracted with QuickExtract DNA Extraction Solution (Lucigen).
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bioRxiv - Genomics 2024Quote: ... High molecular weight DNA was extracted with an optimized MasterPure™ Complete DNA and RNA purification kit (Lucigen, US) with an additional bead-beating step included ...
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bioRxiv - Immunology 2024Quote: ... and in vitro transcribed with AmpliScribe T7 flash transcription kit (Epicentre) as previously described 18,31 ...