Labshake search
Citations for Lucigen :
651 - 700 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... subtilis CU1065 genomic DNA prepared using a DNA extraction kit (QuickExtract, Epicentre). PCR amplification was performed using primers YtqA_F and YtqB_R (Table S3 ...
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bioRxiv - Immunology 2023Quote: The pJAZZ-OK-based linear bacterial plasmids (Lucigen) with coding regions of every HAs were used as templates for mRNAs production ...
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bioRxiv - Cancer Biology 2023Quote: ... Fragments were circularized by CircLigase II (Epicentre), recovered by ethanol precipitation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were maintained under regular media exchange until reaching ∼90% confluency when editing was analyzed by extracting genomic DNA using QuickExtract (Lucigen).
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bioRxiv - Synthetic Biology 2023Quote: ... cloni 10G (Lucigen, Middleton WI) was used for cloning and plasmid propagation ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was circularized using 0.5 uL CircLigase (Epicentre), 1 uL CircLigase buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA in 15 μL of 1× Ampligase DNA Ligase Buffer (Epicentre) containing 250 ng of unshared E ...
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bioRxiv - Synthetic Biology 2023Quote: ... was constructed from the linear pSMART-HCKan vector supplied in the Lucigen CloneSmart kit (Lucigen, Middleton WI, Cat#40708-2) with a single ligation step ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (QE0905T; Lucigen), and PCR products containing the site of Cas9 targeting site were generated using the following primers ...
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bioRxiv - Genetics 2023Quote: ... then electroporated 10 uL into Endura competent cells (Lucigen #602422) and then expanded the cells in liquid culture for 18 hours at 30°C and purified library plasmid with the Nucleobond Xtra Midi EF kit (Machery-Nagel #740420.50) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of total RNA was treated with the RNA processing enzyme RNA 5′-polyphosphatase (Epicentre) to convert 5′-triphosphate RNA or 5′-diphosphorylated RNA to 5′-monophosphate RNA without dephosphorylating monophosphorylated RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... A piece of embryonic tail was taken from each embryo and DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: DNA was isolated from fixed and sorted cells using the MasterPure DNA purification kit (MC85200, Lucigen, LGC Ltd, Teddington, UK) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... cloni 10G SUPREME electrocompetent cells (Lucigen Cat#60080-2) using a MicroPulser Electroporator (BioRad) ...
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bioRxiv - Genomics 2023Quote: ... the media was removed and genomic DNA was extracted using 30 uL/well of QuickExtract DNA Extraction Solution (Lucigen #QE09050) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... cells were washed once with 500 µl PBS and genomic DNA was harvested using QuickExtract DNA extraction solution (Epicentre) following manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... 4 units of Ampligase® DNA Ligase (Epicentre), 0.2 ul of Ampligase® 10X Reaction Buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... the 3′ ends of small RNA were ligated to an adapter using T4 truncated RNA ligase (Lucigen, Cat# LR2D11310K and NEB, Cat# M0242L), followed by the ligation of the 5′-end adaptors by T4 ligase ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Immunology 2023Quote: Bacterial DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), following the manufacturer’s established protocol ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Genetics 2023Quote: ... and extracted genomic DNA with QuickExtract DNA Extraction Solution (QE09050, Lucigen). PCR on the genomic DNA was performed to identify the presence of chromosomes with or without reporter integration56 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2min at 72 C) using the EconoTaq Plus protocol (Lucigen, Middleton, WI, United States). The PCR products were then digested ...
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bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ... -
bioRxiv - Evolutionary Biology 2023Quote: ... cloni 10G (Lucigen, Middleton, WI, United States) reliably yielded >20 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA (rRNA) was depleted from each RNA sample using the Ribo-ZerorRNA Removal Kit (Epicentre), designed for human ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from concentrated cells using the MasterPure complete DNA and RNA purification kit (Epicentre) and sequenced using P6 chemistry on a RS II instrument (Pacific Biosystems) ...
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bioRxiv - Genomics 2023Quote: The cDNA was circularized by incubation with Circligase (Lucigen) for 3 h at 60 °C in a 10 µL reaction as described54 ...
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bioRxiv - Genomics 2023Quote: ... Linearized plasmids were used for in vitro transcription with the AmpliScribe T7-Flash Transcription Kit (Lucigen, ASF3507) using 5-Methyluridine-5’-Triphosphate (5-mUTP ...
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bioRxiv - Plant Biology 2023Quote: ... the RNA samples were used for library construction using ScriptMiner Small RNA-seq library preparation Kit (Epicentre) and sequencing was performed on an Illumina NextSeq500.
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bioRxiv - Neuroscience 2023Quote: 10ug of Trizol-extracted RNA from mouse cortex and striatum was treated with Terminator 5’-Phosphate dependent exonuclease (Lucigen TER51020) according to manufacturer’s protocol. ...
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bioRxiv - Neuroscience 2023Quote: ... transposable element insertional mutagenesis using EZ-Tn5™
Insertion Kit (Epicentre) and sequencing were performed ... -
bioRxiv - Systems Biology 2023Quote: ... with 10 µL RT mix (5 µL 5x Maxima RT Buffer, 1.25 µL 10 mM/each dNTP [New England Biolabs #N0447S], 0.5 µL Lucigen NxGen RNase Inhibitor [Lucigen #30281–1] ...
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bioRxiv - Systems Biology 2023Quote: ... with 10 µL RT mix (5 µL 5x Maxima RT Buffer, 1.25 µL 10 mM/each dNTP [New England Biolabs #N0447S], 0.5 µL Lucigen NxGen RNase Inhibitor [Lucigen #30281–1] ...
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bioRxiv - Pathology 2023Quote: ... coli strains DH5α and C41(DE3) (Lucigen, Middleton, WI) were grown on Luria agar medium at 37 ℃ ...
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bioRxiv - Pathology 2023Quote: ... coli C41 cells (Lucigen). XeAvrRxo1:Xe4429 and Xe4429 proteins were expressed and purified following a procedure as previously described (Han et al. ...
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bioRxiv - Plant Biology 2023Quote: ... with NxSeq AmpFREE Low DNA Library Kits (Lucigen). After size selection by AMPureXP beads ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of circularization reaction mix containing 5 units/µL CircLigase II (Lucigen, CL9021K), 1× CircLigase II buffer (Lucigen ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM KCl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA samples were depleted of ribosomal RNA with Ribo-ZeroTM Magnetic Gold Kit (cat. # MRZG126 Epicentre). Libraries were constructed on BGI Genomics platform (DNBseq ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using the MasterPure Yeast DNA Purification kit (Epicentre) following the kit’s protocol ...
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bioRxiv - Physiology 2023Quote: ... coli CopyCutter (Lucigen, C400CH10). The sequence was verified using sequencing of the full coding sequence.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli TG1 bacteria (Lucigen, Teddington, UK). Addition of the KM13 helper phage (kindly provided by Pierre Martineau ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Lucigen), and harvested the plasmid DNA using the Qiaprep Spin miniprep kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 μL of Hybridase RNase H (Lucigen H39500), 3 μL of 5× RNase H buffer (167 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 72 µL of either Hybridase Thermostable RNase H (Lucigen H39500) or Thermostable RNase H (NEB M0523 ...