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Citations for Lucigen :
251 - 300 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2024Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI) and vortexed for 10s ...
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bioRxiv - Microbiology 2024Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2024Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from each CFU recovered using the QuickExtract DNA extraction solution (Epicentre, # QE09050) and each colony was screened for the presence of tbpA and lbpA genes ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Microbiology 2024Quote: We performed Colony PCR on the overnight liquid culture of 451 isolates in a 25μL reaction containing 12.5μL EconoTaq® PLUS 2X PCR Master Mix by Lucigen, 1μL of 10μM 27F 16S rRNA primer ...
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bioRxiv - Microbiology 2024Quote: ... 52 units/mL Ready-Lyse (Epicentre)] and incubated 15 minutes at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cells with QuickExtract (Lucigen #QE0905), column-purified (Zymo Research #C1102-50) ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were recovered by centrifugation and pellets were flash frozen until resuspension in 40 μl of a 20 mg/ml proteinase K solution (Avantor®, Radnor, PA, USA) with 1 μl of undiluted Ready-Lyse Lysozyme solution (Lucigen®, Middlesex, UK), and lysis was allowed to proceed for 10 min in a shaking incubator at 37 °C and 600 rpm ...
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bioRxiv - Immunology 2024Quote: ... and plasmid-safe ATP-dependent DNase (Epicentre, USA, Cat: E3101K) mix at 37 °C for 30 min to remove RNAs and linear genomic DNA according to the instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and C41(DE3) (Lucigen) respectively.
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. All samples were treated with Invitrogen TURBO DNA-Free kit to remove DNA contamination ...
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bioRxiv - Microbiology 2024Quote: ... The EZ-Tn5
Tnp transposome complex (Epicentre BioTechnologies) was added to the electrocompetent cells ... -
bioRxiv - Microbiology 2024Quote: ... 5’ polyphosphatase was purchased from Epicentre (RP8092). The mRNA decapping enzyme was purchased from New England Biolabs (M0608S) ...
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bioRxiv - Microbiology 2024Quote: ... The Ribo-Zero rRNA Removal Kit (Epicentre, USA) was used for DENV2-infected cellular RNA to remove rRNA ...
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bioRxiv - Neuroscience 2024Quote: ... and genomic DNA was extracted using QuickExtract (Lucigen Cat # QE09050), and a PCR over the region of interest was then conducted ...
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bioRxiv - Microbiology 2024Quote: Microbial DNA extraction was performed following the MasterPure Complete DNA and RNA Purification Kit (Epicentre, Southampton, Hampshire, UK) and Pathogen Lysis Tubes (QIAGEN ...
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bioRxiv - Neuroscience 2024Quote: ... Ribo-Zero-selected (Epicentre, Madison, WI), DNase-treated (Roche) ...
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bioRxiv - Synthetic Biology 2024Quote: ... We confirmed successful genomic editing by extracting genomic DNA from at least 104 harvested cells using the QuickExtract protocol (Lucigen, QE09050). Correct HDR template integration was validated through Sanger sequencing of the PCR amplification of the target locus with at least one primer annealing outside the integration site.
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using MMLV reverse transcription (Lucigen). 20 ng of cDNA/reaction was used in subsequent PCR ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from pure yeast colonies with the MasterPure™ Yeast DNA Purification Kit (Epicentre®, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stored either in 30 μL 2xT&C lysis buffer of the MasterPure Complete DNA & RNA Purification Kit (Lucigen, Wisconsin, USA) or in 10 μL PCR-grade water ...
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bioRxiv - Microbiology 2024Quote: ... with EconoTaq DNA polymerase (Lucigen) performed on the S1000 Therma Cycler (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... The fragmented DNA was end-repaired using the End-It kit (Lucigen) and A-tailed using Klenow fragment (3’-5’ exo- ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.2U/μL RNAse inhibitor (Lucigen #30281-1) for subsequent processing by flow cytometry.
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bioRxiv - Biophysics 2024Quote: ... In vitro transcription reactions were run using the AmpliScribe T7 High Yield Transcription Kit (Lucigen AS3107) for 4 hours at 42°C ...
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bioRxiv - Plant Biology 2024Quote: ... and Ribo-Zero rRNA Removal kit (Epicentre), respectively ...
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bioRxiv - Genetics 2024Quote: ... media aspirated and 30uL of QuickExtract™ DNA Extraction Solution (Lucigen) added to the wells ...
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bioRxiv - Microbiology 2024Quote: ... 3 mL of saturated culture was pelleted and used according to manufacturer instructions following the MasterPure Complete DNA and RNA Purification Kit (Lucigen MC85200). Isolated genomic DNA was quantified using a NanoDrop and used as input for the Illumina DNA Prep Tagmentation Kit (formerly Nextera DNA Flex ...
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bioRxiv - Bioengineering 2024Quote: ... in Ampligase buffer (Lucigen)) for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... coli C43(DE3) cells (Lucigen) with GFP fusion on the C-terminus and solubilised from the membrane fraction by n-dodecyl-β-D-maltopyranoside (DDM) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from three individual cultures (triplicates) using the MasterPure Yeast RNA Purification Kit (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: Endura electrocompetent cells (Lucigen 60242-2)
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bioRxiv - Cell Biology 2024Quote: ... coli HI-control BL21(DE3) (Lucigen, USA) cells were used for expression using Isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2024Quote: ... 171-304 of Drosophila PKC98E) and C1 delta (a.a. 152-280 of mouse PKC delta) genes were cloned into pETite vector (Lucigen, USA) with a C-terminal TEV cleavage site ...
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bioRxiv - Microbiology 2024Quote: ... The eluted vRNA was treated with 10 U of RNaseR (Lucigen, Middleton, WI) in an RNaseR buffer (20 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Microbiology 2024Quote: ... Note that pSMART (Lucigen), pMR120 ...
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bioRxiv - Microbiology 2024Quote: ... cloni replicator cells (Lucigen) are transformed with these plasmids and cultured with arabinose induction to increase the copy number for plasmid isolation.
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bioRxiv - Immunology 2024Quote: ... cDNA was circularised with CircLigase II (Epicentre), amplified by PCR using Solexa P5/P7 primers and sequenced in a DNBseq platform from BGI Genomics (100bp ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli strain EPI300 (LGC Biosearch Technologies, Lucigen, catalog number: EC300110) was grown in lysogeny broth (Miller formula ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from single cell clones using QuickExtract DNA Extraction Solution 1.0 (Lucigen) and the region of interest was amplified by two consecutive PCRs ...
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bioRxiv - Microbiology 2024Quote: DNA isolation was performed using Epicentre MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Systems Biology 2024Quote: ... 800 mM guanidine HCl) and 0.1ug/μL proteinase K (Epicentre MPRK092). Sample was then incubated at 65 C for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Epicentre MasterPure Complete DNA and RNA Purification Kit (Lucigen, Middleton WI) following the DNA purification protocol for cell samples and quantified using the Qubit dsDNA high-sensitivity assay protocol and a Qubit 3.0 fluorimeter (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... along with 375 U/μL lysozyme (Epicentre, R1810M), and incubated at 37C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... was incubated with Terminator™ 5′-Phosphate Dependent Exonuclease (TEX, Epicentre) or left untreated at 37° C for one hour as described (61) ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions (Lucigen). In all cases ...
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bioRxiv - Systems Biology 2024Quote: ... and self-ligated using Fast-link ligase (LK0750H; Lucigen). Duplets of 800bp-1000bp were excised from agarose gel ...