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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 30ml of ExpiCHO-S cells at 6M cells/mL were transfected with 1ug/mL of the spike WT using the ExpiCHOTM Expression System Kit (Gibco, catalog # A29133) following the manufacturer’s Standard protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequence and gene annotation of the mitochondrial genome assembly (NCBI GenBank, AY659991.1) and RNA spike-in control sequences (ERCC, Ambion; for quality control) were included as well ...
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bioRxiv - Genomics 2023Quote: ... and External RNA Control Consortium (ERCC) spike-in transcripts were added to M8 and D6 samples at manufacturer recommended amounts (4456740, Thermo Fisher Scientific) 13 ...
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bioRxiv - Immunology 2023Quote: ... The ectodomain of ancestral SARS-CoV-2 spike (GenBank: MN908947.3) was expressed as previously described.10 The protein was purified from FreeStyle 293-F cells (Thermo Fisher Scientific, USA) using affinity chromatography followed by size exclusion chromatography ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix (Invitrogen, cat# 4456740. 1:1,000 dilution). All libraries were sequenced on the Illumina NovaSeq 6000 system.
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bioRxiv - Cell Biology 2020Quote: ... T1249-Fc were expressed by transient transfection in 293-6E (CNRC) or Expi293 (ThermoFisher Scientific) cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... absorbance at 405 nm was measured in a MultiSkan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... 10% FCS and 100 units / ml of penicillin and 100 μg / ml of streptomycin (Gibco). U87MG ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-rabbit IgG Fc secondary antibody (Invitrogen, Cat. No. 31463).
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at 570 nm was measured using a spectrophotometer (Multiskan FC Microplate Photometer, Thermo Fisher). A standard curve was generated using standard glycogen solution in the reaction ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Cells were infected with the specified viruses and MOIs in DMEM without FCS (Life Technologies) for 1h at 37.0°C (Cts24 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) for 3 hours before fixation for 15 minutes (3.7% FA and 0.5% triton X-100) ...
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bioRxiv - Molecular Biology 2022Quote: ... and then blocked in PBS supplemented with 20% foetal calf serum (FCS, Thermo Fisher Scientific) and 20% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen, Waltham, Massachusetts, United States) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Absorbance at 405 nm was read on a Multiskan FC plate reader (Thermo Scientific, USA).
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bioRxiv - Biochemistry 2020Quote: ... SKOV3-cells were cultured in McCoy’s 5A Medium supplemented with 10% FCS (Thermo Fisher Scientific). HROC24-cells were cultured in DMEM/Ham’s F12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1mM sodium pyruvate and 3.7 g/L NaHCO3 and supplemented with 15% FCS (Gibco, A3160801), 10 mM HEPES (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked with anti-mouse CD16/CD32 (20 µg/ml, clone 93; Invitrogen) for 20min ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin (P/S; Thermo Fisher) in a humidified atmosphere at 37°C with 5% CO2 ...
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bioRxiv - Physiology 2019Quote: ... Cells were kept in DMEM supplemented with 10% tetracycline-free Hyclone™ FCS (Fisher Scientific) and the above-mentioned antibiotics.
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice following staining and resuspended in FC buffer containing 7AAD (Life Technologies) or FVS700 (BD Bioscience ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at 450 nm by a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... supplemented with 4% FCS and 0.75% sodium bicarbonate containing 0.5% low melting agarose (Invitrogen, USA). The overlay was allowed to solidify at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS) and 2mM L-glutamine (all from Life Technologies). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% foetal calf serum (FCS) and 1mM Sodium Pyruvate (all from Life Technologies). DLD-1 and COLO205 cells were maintained in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Biochemistry 2020Quote: ... The absorbance was measured with a Multiscan™ FC microplate photometer (Thermo Fisher Scientific, USA). Cell viability is presented as the percentage of control (untreated cells) ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme and Fc-portion was removed on HIS-Pur Ni-NTA resin (Thermo Fisher Scientific) and Protein G sepharose (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% inactivated fetal calf serum (FCS) (Cat: a3520501 Thermo Fisher Scientific Inc, USA), an 1% antibiotic mixture (Cat ...
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bioRxiv - Immunology 2020Quote: ... DMEM/HAM’s F12 (FAD) medium with low Ca2+ (50 µM) supplemented with 10% FCS (Gibco) treated with chelex 100 resin (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... When needed neutrophils were cultured in RPMI 1640 containing 10% fetal calf serum (FCS, Gibco), 2 mM L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Immunology 2020Quote: ... Fc-fusion proteins were expressed via transient transfection of suspension-cultured CHO-S (Invitrogen/ThermoFisher) cells using the Mirus Bio ...
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bioRxiv - Microbiology 2021Quote: ... bacterial growth was examined by measuring the A600 optical density (Multiskan FC, Thermo Fisher Scientific). All measurements were performed at least in triplicates ...
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was chemically biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... all cells were suspended in Hanks’ balanced-salt solution (HBSS) supplemented with 1% FCS (GIBCO). Embryonic tissues were micro dissected under a binocular magnifying lens ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
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bioRxiv - Immunology 2022Quote: 293TN HEK cells for lentiviral production were cultured in DMEM medium with 10% FCS (Gibco) and 1 mM sodium pyruvate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added and the signal was measured in MultiSkan FC microplate reader (Thermo Fisher, USA) at 530/590 nm.
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bioRxiv - Microbiology 2022Quote: ... 1:500 dilution) (goat anti-human IgG Fc Alexa 488 conjugated, Thermo Fisher, Cat #: H10120) (goat anti-human IgG (H + L ...
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bioRxiv - Plant Biology 2022Quote: ... and 666 nm in a Multiskan FC microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). Total chlorophyll (Chl a + Chl b ...