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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). The assay was carried out by placing the Streptavidin Biosensors (ForteBio ...
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bioRxiv - Immunology 2020Quote: Transduced T cells were labelled with anti-mouse TCRβ-Biotin (clone H57-597, Thermo Fisher Scientific, USA) for EBV/LMP2-TCR or anti-TCRVβ13.1-PE (clone H131 ...
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bioRxiv - Genomics 2021Quote: ... This fragmented BAC DNA was labeled as described elsewhere (Dernburg 2011) using either Biotin-14-dATP (Invitrogen) or Digoxigenin (Dig)-11-UTP (Roche ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were separated from non-bound biotin by Zeba™ Spin Desalting Columns (Thermo Scientific™ 89882). Biotinylation does not interfere with the binding kinetics ...
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bioRxiv - Molecular Biology 2020Quote: EVs were biotinylated using EZ-Link Sulfo-NHS-LC LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA). Biotinylated EVs were loaded onto Zeba spin desalting columns (7 K MWCO ...
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bioRxiv - Molecular Biology 2021Quote: ... Where required antibodies were biotinylated using EZ-link NHS-LC-Biotin according to the manufacturer’s instructions (Thermofisher), or were attached to cyanogen bromide-activated agarose using the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Genetics 2020Quote: T and B lymphocytes were enriched by positive selection using the Dynabeads Biotin Binder kit (Invitrogen, #11047), and biotinylated α-TCRβ and α-CD19 antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated with freshly prepared 0.5 mg/ml EZ-link™ Sulfo-NHS-SS-Biotin (Thermo Scientific) in cold 1X PBS for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated with biotin-labeled goat anti-rabbit IgG (H+L) (1: 500, #31460, ThermoFisher, USA) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: SEC purified PfRH5 in 1X PBS was chemically biotinylated using EZ-LinkTM Sulfo-NHS-Biotin (Thermofisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... adjusted to pH = 7.7) containing 0.5 mg/mL EZ-LinkTM Sulfo-NHS-SS-Biotin (ThermoFisher Scientific, USA) for 20 minutes at 4° C ...
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bioRxiv - Immunology 2023Quote: ... The assay for MUC5AC was performed using biotin-conjugated mouse anti-MUC5AC mAb (45M1, Invitrogen, #MA5-12175) as described previously (12) ...
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bioRxiv - Immunology 2024Quote: ... The RBD-biotin was prebound with streptavidin-AlexaFluor-647 to make RBD-647 (Invitrogen, cat no. S21374). B cells were stained with CD19 ...
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bioRxiv - Immunology 2024Quote: ... cloned and expressed in-house) were biotinylated using EZ-Link™ Sulfo-NHS-Biotin (Thermo Fisher Scientific) with 50-fold molar excess biotin for 48hr at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Restricted-DNA fragments were filled-in with 15 nmol each of biotin-14-dATP (cat. 19524016, Thermofisher), dTTP ...
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bioRxiv - Biochemistry 2024Quote: ... TcA(914Cys) and TcA(1279Cys) were biotinylated using EZ-link NHS-PEG12-Biotin (ThermoFisher, cat. no. A35389). Biotinylation was performed in labelling buffer simultaneous to the labelling reaction with BFL and Atto647N ...
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bioRxiv - Cancer Biology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/ml Premium Grade Sulfo-NHS-LC-Biotin (Fisher Scientific) in PBS at 4°C for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... and the PEG-biotin:streptavidin:FUS-biotin tethering system was established by incubating 1 mg/mL streptavidin (Thermo Fisher) and then 1 µM biotinylated FUS in sample wells for 15 minutes each ...
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bioRxiv - Cell Biology 2023Quote: ... EZ-Link™ Sulpho-NHS-Biotin (1 mg/ml in PBS buffer, pH 8; Thermo fisher; 21217) was added (or not ...
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bioRxiv - Cell Biology 2023Quote: ... total DNA was linearly amplified and biotin-labeled using Illumina TotalPrep kits (Life Technologies, Temecula, CA, USA). The sample quality was measured using an Agilent 2100 Bioanalyzer before being hybridized to Illumina BeadChips ...
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bioRxiv - Neuroscience 2023Quote: ... but with primary antibody targeting polyGA detected by goat anti-mouse IgG1 Biotin (A10519, Thermo Fisher Scientific), followed by 3x 5-min PBS washes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The biotin-dUTP and digoxigenin-dUTP were detected using streptavidin-Alexa 488 (Invitrogen, San Diego, Calif., USA) and anti-digoxigenin-rhodamine (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The biotin-dUTP and digoxigenin-dUTP were detected using streptavidin-Alexa 488 (Invitrogen, San Diego, Calif., USA) and anti-digoxigenin-rhodamine (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488-or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
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bioRxiv - Bioengineering 2022Quote: ... NHS-PEG4-Biotin (catalog no. 21330) and HRP-conjugated streptavidin (catalog no. N100) were purchased from Thermofisher. DAB (catalog no ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated peptides (Biotin-PNTAYQK(Ac)QQDTLI) previously immobilized on magnetic Streptavidin beads (Dynabeads MyOne Invitrogen™ 65601) were incubated in presence or absence of SIRT1 −/+ NAD+ (0.5 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... click chemistry reactions were performed for 1.5 h in the dark (10 μM Biotin-azide [Thermo Fisher, B10184] ...
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bioRxiv - Genomics 2023Quote: ... Restricted-DNA fragments were filled-in with 15 nmol each of biotin-14-dATP (cat. 19524016, Thermofisher), dTTP ...
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bioRxiv - Developmental Biology 2023Quote: Biotin-labeled cDNA was synthesized and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... EZ-Link™ Sulfo-NHS-Biotin (100uL A39256, Thermo Fisher Scientific, 1 mg/0.4 mL in PBS). After 30min circulation ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The bat albumin was biotinylated using EZ-Link® NHS-SS-Biotin (Thermo Fisher Scientific, Ref#21441) following a protocol recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were replenished with a complete medium containing FBS and 50 uM biotin (Thermo Scientific #29129). Cells were lysed 18 hours after treatment for protein collection and analyzed by immunoblotting ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific). Concentrations given are for Ydj1 dimers and for Ssa1 and luciferase monomers.
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Medium was furthermore supplemented with 250 nM Rapalog 1h before harvest and 50 µm Biotin (Invitrogen, B20656) 30 min before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in surface biotinylation buffer (0.2 mg/ml sulfo-NHS-SS-biotin in DPBS, Thermofisher#A39258) for 45 minutes at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... EdU was chemically linked to Biotin-azide using the Click-iT™ Reaction Kit (Thermo fisher scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant single-strand AAV vectors were produced using the ultracentrifugation method described in a previous paper were produced using HEK293-T cells (HCL4517; Thermo Fisher Scientific; Waltham, MA), as described previously26 ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Genomics 2020Quote: ... at each time point using trizol in the presence of 1μl of a 1/10 dilution of ERCC Spike-In RNA (Thermo Fisher Scientific, Cat: 4456740). To monitor mRNA stability in conditions where mRNA translation is impaired ...
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bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...