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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% v/v Fetal Calf Serum (FCS; Gibco/Life Technologies Ltd, Paisley, UK) (Supplementary Table S1 ...
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bioRxiv - Immunology 2022Quote: ... The eOD-GT8 Fc was conjugated with Alexa Fluor 647 fluorescence (Thermo Fisher Scientific A30009). The ΔeOD-GT8 Fc was conjugated with Biotin (Thermo Fisher Scientific A30010 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% v/v Fetal Calf Serum (FCS; Gibco/Life Technologies Ltd, Paisley, UK) (Supplementary Table S1 ...
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bioRxiv - Physiology 2024Quote: NIH-3T3 fibroblasts (3T3-FB, 1.0×104 cells) were cultured in 10 % FCS/DMEM (Gibco, Life Technologies ...
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bioRxiv - Genetics 2024Quote: ... We measured the absorbance at 492 nm in a microplate reader (Thermo Scientific Multiskan FC). Plotting of graphs and statistical analyses were conducted with GraphPad Prism 8.4.2.
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed every hour using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 620 nm.
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Capricorn) and 1% penicillin/streptomycin (Gibco). Huh 7.5 cells were maintained in complete DMEM medium supplemented with non-essential amino acids.
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS and 1 U/mL Penicillin-Streptomycin (10 000 U/mL, Gibco) in 96 well round-bottom plates ...
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bioRxiv - Cell Biology 2023Quote: ... MDA-MB-231 cells were grown in DMEM/F12 with 10% FCS (Thermo Fisher Scientific) and 100 U μL/mL penicillin and 100 μL/mL streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... The data was immediately collected by Multiskan™ FC Microplate Photometer (Thermo Fisher Scientific, USA) at 450 nm ...
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bioRxiv - Plant Biology 2023Quote: ... Absorbance at 340 nm was monitored continuously with a spectrophotometer (MULTISKAN FC; Thermo Fisher Scientific) to detect NADH production.
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bioRxiv - Microbiology 2023Quote: ... The absorbance was determined at 450 nm using a microplate reader (Thermo Scientific Multiskan FC). All experiments were carried out in four biological replicates.
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bioRxiv - Immunology 2023Quote: TAP2 deficient CHO cells were cultured in complete RPMI (Invitrogen, 2mM glutamine and 10% FCS) and stably-transfected with >10g plasmid DNA using poly-L-ornithine and a 30% DMSO shock ...
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bioRxiv - Bioengineering 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies (ab97250 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were treated with anti-CD16/32 (Fc block) (14-9161-73, ThermoFisher) and stained with the live/dead marker ethidium monoazide bromide (ThermoFisher #E1374 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (590nm excitation and 620nm emission) with Multiskan FC (Thermo Scientific) [33 ...
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bioRxiv - Immunology 2023Quote: ... was measured at 180 minutes (544nm excitation and 590nm emission) with Multiskan FC (Thermo Scientific) [31].
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% L-glutamine (Gibco™ 25030-024). The human nasal septum epithelial cell line RPMI 2650 (ATCC CCL-30 ...
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bioRxiv - Physiology 2023Quote: ... and blocked on ice for 20 minutes with Fc Block anti-CD16/CD32 (Thermo Fisher). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... OD600 measurement in a microplate reader (Multiskan™ FC, Thermo Scientific, Etten-Leur, the Netherlands) where performed ...
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bioRxiv - Microbiology 2023Quote: ... Optical density was measured at 450 nanometer using a Multiskan FC machine (Thermo Scientific, USA) in combination with Mikrowin (software version 5.09 ...
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bioRxiv - Cancer Biology 2023Quote: Hap1 cells (Horizon Discovery) were maintained in IMDM supplemented with GlutaMAX and 10% FCS (Gibco). Cells regularly tested negative for mycoplasma contamination.
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bioRxiv - Immunology 2023Quote: ... Pheonix cell and LS174T cells were cultured in complete DMEM (Gibco, 10% FCS, 2mM GlutaMAX).
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bioRxiv - Immunology 2023Quote: ... Samples were then incubated with an Fc receptor binding inhibitor polyclonal antibody (Thermo Fisher Scientific), before staining with a surface antibody cocktail (Supplementary Table 1) ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Alexa Fluor 488 Anti-alpha 1 Sodium Potassium ATPase antibody (ab197496) ...
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bioRxiv - Immunology 2024Quote: ... followed by Fc-Block step with anti-mo CD16/CD32 (Invitrogen, Cat# 14-01061-86) and staining of surface markers with a mixture of conjugated antibodies 1:250 in 2%FBS PBS 1mM EDTA buffer with 10% Brilliant Stain Buffer Plus (BD ...
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bioRxiv - Plant Biology 2024Quote: ... and reading absorbance at 620 nm in a photometer (Multiskan FC, Thermo Scientific, United States) and using a glucose standard curve to compare the colorimetric response of the samples ...
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bioRxiv - Immunology 2024Quote: ... followed by absorbance measurements at 450nm using a Multiskan FC Microplate Photometer (51119000; Thermo Scientific). As a positive control ...
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) HRP (Horseradish peroxidase) was purchased from Invitrogen. Goat anti-mouse IgG1 and IgG2c Fc secondary antibodies with HRP (ab97250 ...
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bioRxiv - Immunology 2024Quote: ... Fc receptors were then blocked with anti-mouse CD16/CD32 (Invitrogen, Cat# 14-01061-86) along with extracellular staining with a mixture of conjugated antibodies in FACS buffer (1:250 for LP cells ...
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bioRxiv - Immunology 2024Quote: ... Absorbance was detected at 450 nm by the Multiscan FC (Thermo Fisher, Waltham, MA, USA).
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bioRxiv - Bioengineering 2024Quote: ... Goat anti-mouse IgG Fc secondary antibody (A16084) horseradish peroxidase (HRP) was purchased from Invitrogen and 3,3″,5,5″-Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2024Quote: ... + 10% FCS + 1% GlutaMAX + penicillin-streptomycin (P/S, 12.8 U/mL, Gibco, cat.15140-122) supplemented with 10% DMSO at -150°C ...
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bioRxiv - Immunology 2024Quote: ... containing 10% FCS (Biochrome, Cat. S0615-500ML) and 1% Penicillin/ Streptomycin (Thermo Fisher, Cat. 7001592). After sorting ...
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bioRxiv - Microbiology 2019Quote: Human primary B cells from adenoids were separated from T cells by rosetting with sheep erythrocytes (purchased from Thermo Scientific Oxoid, cat. no. SR0051D) and purified by Ficoll density gradient centrifugation as described in the previous paragraph ...
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bioRxiv - Genomics 2020Quote: ... then 2 h with secondary antibodies at 1:500 dilution (anti-mouse Alexa488-conjugated Life technologies cat #A21202 and anti-sheep Alexa555-conjugated Life technologies cat #A21436) and washed 6 times in PBT ...
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bioRxiv - Neuroscience 2023Quote: ... specific fluorescent immunostainings were visualized using Alexa488-conjugated anti-sheep IgG and Alexa546-conjugated anti-rabbit IgG (1:300; Molecular Probes, Eugene, OR, USA).
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bioRxiv - Bioengineering 2022Quote: ... were used to normalize expression levels of eight osteogenic marker genes (RUNX2, BGLAP, DMP1, ALPL, PDPN, SOST, COL1a2, MMP14) which were analyzed with specific TaqMan probes (Applied Biosystems, see Table S1).
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bioRxiv - Cell Biology 2022Quote: Primary human lung tissue from post-mortem or surgical resection donors (detailed in supplemental table S1) was fixed in 10% neutral buffered Formalin (Thermo Fisher Scientific, Waltham, MA). The tissue was then dehydrated in 70% ethanol (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... were plated into each well of a 24-well plate and transfected with 50 ng pCAG-hyPBase (Ochiai et al., 2015) and 450 ng Intein-related plasmids (Tables S1 and S2) using Lipofectamine 3000 (L3000015, Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Coding sequences of mature proteins without signal peptide were amplified with primers listed in Table S1 and cloned into expression vector pETSUMO (Thermo Fisher Scientific, Massachusetts, USA). Correctness of the resulting constructs pETSUMO-ChElp2 and pETSUMO-Vd2LysM were confirmed by DNA sequencing and introduced into E ...
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bioRxiv - Systems Biology 2022Quote: The Headspace-SESI system consists of an in-house developed custom-made headspace sampling system (Fig 1C and Fig S1, adapted from [61]), a commercial SESI source (SuperSESI, Fossiliontech, Spain) and an Orbitrap mass spectrometer (Q-Exactive Plus, Thermo Scientific, San Jose, CA). All three EAM strains were able to grow in the Headspace-SESI system under the measurement condition (Fig S4A) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected with the PiggyBac or Tol2 transposase expression vector (mPBase or pCAGGS-T2TP; Table S1) and the donor vectors using 293fectin (Thermo Fisher Scientific, Waltham, MA). One day after tge transfections ...
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bioRxiv - Developmental Biology 2019Quote: ... A single-stranded oligonucleotide with 50 bp arms for HDR (Supplementary Table S1) was also delivered with PX459 using the Neon® Transfection System (Thermo Fisher, New Zealand) at 1,500 V with one 20 ms pulse ...
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bioRxiv - Plant Biology 2021Quote: ... fragments were purified and used as templates for in vitro transcription. The products of RNA transcripts were confirmed by gel electrophoresis (Supplementary Fig. S1) and quantified by NanoDrop (Thermo Fisher Scientific, Waltham, USA). We equally mixed 3 fragments and used for SIGS assay on polyethylene tape ...
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bioRxiv - Physiology 2023Quote: ... The gene expression level relative to β-actin was determined by qPCR with gene-specific primers (Supplementary Table S1) and PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, USA). 2-ΔΔCT was calculated for relative fold change.