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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Amplification and biotin labeling of RNA was performed using GeneChip 3’ IVT express kit (Affymetrix, #901229). Affymetrix Drosophila Genome 2.0 array (# 900533 ...
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bioRxiv - Pathology 2022Quote: ... EpCAM and Ter119-biotin antibodies (1:200) and Dynabeads MyOne Streptavidin T1 (40 μl / sample, Invitrogen). After magnetic negative selection ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were blocked 10% biotin-free fetal bovine serum (FBS, Fisher Scientific, Cat# 26-400-044) in TBST solution overnight at 4°C with mild agitation ...
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bioRxiv - Neuroscience 2022Quote: ... visualized by incubation with avidin-biotin-peroxidase complex (Immunopure ABC Peroxidase staining kit; Thermo Fisher Scientific), using the VectorSG Peroxidase Substrate Kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Molecular Biology 2024Quote: ... Reducing agents were then removed by filtering through Pierce dye and biotin removal column (Thermo Scientific). The resulting chains were then incubated overnight at room temperature with 10x molar excess of EZ-Link Maleimid-PEG2-Biotin (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... after which free biotin was removed with 7K MWCO Zeba desalting spin columns (Thermo Fisher Scientific). Costar high binding 96-well assay plates (Corning ...
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bioRxiv - Molecular Biology 2022Quote: ... EdU-PEG3-Biotin containing DNA molecules were affinity purified by Streptavidin MyOne C1 Magnetic Beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The biotin-labeled probes were detected using a Chemiluminescent Nucleic Acid Detection Module (Thermo Scientific™) at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and the biotin-labeled mRNA was detected by streptavidin Alexa Fluor™ 647 conjugate (Invitrogen™). To investigate the effect of IbpBEc on IbpAEc-mRNA interaction ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-labeled RNA was then biotinylated with 1mg/ml EZ-link HPDP-Biotin (ThermoFisher, cat: 21341) for 90 min at room temperature as previously described34 ...
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bioRxiv - Developmental Biology 2023Quote: Primary antibodies and dilutions used in this study: mouse (biotin)-α-HuC/D (1:1000. Invitrogen), goat-α-RFP (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... the overhangs in the restriction digested chromatin were filled in with biotin-14-dATP (ThermoFisher Scientific) using 30 U of DNA Polymerase I ...
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bioRxiv - Immunology 2023Quote: ... These antibodies were biotinylated using the EZ-Link Sulfo-NHS-LC-Biotin Kit (Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... as previously done.26 AChR recycling was assessed by injecting α-bungarotoxin-Biotin (25 pmoles - Invitrogen) and saturating dose of streptavidin-Alexa647 (50 pmoles - Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylation of the purified 3FTX was confirmed using the Pierce™ biotin quantitation kit (Thermo Fisher). Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... Appropriately-sized ligation fragments marked by biotin were pulled down with MyOne Streptavidin C1 DynaBeads (Invitrogen) and prepped for Illumina sequencing.
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bioRxiv - Biochemistry 2023Quote: ... and the biotin-labeled mRNA was detected by streptavidin Alexa Fluor™ 647 conjugate (Invitrogen™).
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bioRxiv - Genetics 2023Quote: ... and treated with 0.3 mg/ml EZ-Link Sulfo-NHS Biotin (Thermo Fisher, Waltham, MA, USA) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... for GFP and a biotin-coupled goat anti-rabbit antibody (1:500; catalog #A-16027, Invitrogen) for VIP at room temperature for 120 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Smurf1 HECT NT protein was biotinylated using the No-Weight NHS-PEO4-Biotin kit (Thermo Fisher) due to its compatibility with Super Streptavidin (SSA ...
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bioRxiv - Genomics 2024Quote: ... Biotin enrichment was then performed by adding 20 μl of Dynabeads MyOne Streptavidin C1 (ThermoFisher 65001) and incubating at room temperature for 30 minutes with rotation ...
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bioRxiv - Microbiology 2024Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific) as reported previously 70.
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bioRxiv - Microbiology 2021Quote: Well-differentiated camelid AEC cultures were infected with 4000 PFU of MERS-CoV (strain EMC/2012, propagated on Huh7 cells diluted in Hanks balanced salt solution (HBSS, Gibco). The cells were washed with 100 μl of HBSS prior to infection (18) ...
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bioRxiv - Molecular Biology 2022Quote: ... 127 pg of REBOV Vp40-pGEM-T Easy and 7.7 pg of MERS-CoV UpE-p GEM-T Easy using 2X Verso hot-start PCR buffer (Thermo Scientific). The reaction was set up in 15 μl final volume and the thermal cycling was as follows ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Genomics 2022Quote: Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer supplemented with 5 µl protease inhibitor cocktail (Thermo Fisher cat# 87786) and 10 µl Rnase inhibitor (NEB cat# M0314B ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-Hep1, Huh7, MHCC-97H, and HEK293 cells were cultured in DMEM (HyClone, Logan, UT, USA) supplemented with 10% FBS (Gibco, Rockville, MD, USA), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing CMV:DOR-APEX2 were created as previously described and maintained in 1:1000 G418/Geneticin (Thermo Fisher Scientific, Cat # 10131035) (Lobingier et al ...
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bioRxiv - Immunology 2023Quote: ... Gregoire Lauvau’s lab) and HEK293 Phoenix Ampho Packaging Cells (provided by Prof. Andrew Ward) were cultured in complete DMEM medium (Gibco, Cat#10569-010) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC; CRL-1573™) were cultured in complete DMEM (cDMEM) in Nunc EasYFlask T75 cell-culture flasks (Thermo Scientific; cat # 156499) and housed in CO2 incubator (5% CO2 ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2µl lysis buffer with 1U/µl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng of total RNA were mixed with 1 µL of a 1:10 dilution of ERCC RNA Spike-In Mix controls (Thermo Fisher 4456740). ERCC mix 1 was added to HEK293T and HEK293T-Nsp1 and mix 2 was added to HEK293T-Nsp2 samples ...
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bioRxiv - Synthetic Biology 2021Quote: ExpiCHO-S cells (6E6cells/mL) were transfected with 1ug/mL of Spike protein DNA22 using the ExpiCHO™ Expression System Kit (Gibco #A29133) and shaken at 37°C and 8% CO2 in a 50mL culture ...
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bioRxiv - Developmental Biology 2020Quote: ... 500ng of RNA was used to prepare RNA-seq libraries and spiked-in with ERCC Exfold Spike-in mixes (Thermo Fisher, 4456739). RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Preparation kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured in a Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% FBS and transfected by pLV-Spike using a calcium phosphate kit (ThermoFisher, Waltham, MA, USA). The control virus with VSV-G as the tropism and expression of mCherry was generated by co-transfection of pLV-mCherry and pMD2.G vector (Addgene ...
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bioRxiv - Biochemistry 2020Quote: ... different domains or subunits of the Spike protein and the extracellular domain of ACE2 were produced in Expi293F cells (Thermo Fisher Scientific). Expi293F cells were cultured at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The final Spike protein concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Final proteins were dispensed in aliquots of 0.5 ml each and stored at -80°C prior to use.
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bioRxiv - Genomics 2022Quote: ... Human cell lysate and mouse cell lysate (as a spike-in internal control) were combined (∼4:1 ratio) and incubated with protein A or G Dynabeads (Thermo Fisher Scientific) conjugated with the relevant antibodies for 14 h at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Reads that did not map to rRNA were then mapped with STAR (2.5.3a) to the full reference genome (UCSC build GRCm38/mm10) (including the RNA spike-in control sequence ERCC92, ThermoFisher cat. no. 4456740) using the following parameters ...
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bioRxiv - Molecular Biology 2019Quote: ... Nanopore libraries were prepared from 1 μg poly(A)+ RNA combined with 1 μl undiluted ERCC RNA Spike-In mix (Thermo Fisher Scientific) using the nanopore DRS Kit (SQK-RNA001 ...
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bioRxiv - Immunology 2020Quote: ... Fragmented chromatin from G1-arrested cells with or without spike-in was precleared with 40 μl Dynabeads Protein A (ThermoFisher Scientific, #10002D) and then incubated with Rad21 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: 10 ng total RNA containing 5 pM ath-miR-159a spike-in was reverse-transcribed using Taqman Advanced miRNA cDNA synthesis kit (Applied Biosystems #A28007) and miRNAs were detected using specific probes for ath-miR159a (478411_mir) ...
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bioRxiv - Systems Biology 2020Quote: RNA-Seq libraries were prepared from 65 ng of total RNA and 1 μL of 1:5000 (v/v) dilution of ERCC ExFold RNA Spike-In Mix 1 (Thermo Fisher Scientific) with TruSeq® Stranded mRNA Library Prep for NeoPrep™ instrument as per the NeoPrep Library Prep System Guide on the instrument (Illumina).
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bioRxiv - Biochemistry 2020Quote: The ACE2 and Spike proteins were expressed and purified from Expi293 BirA cells according to established protocol from the manufacturer (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequence and gene annotation of the mitochondrial genome assembly (NCBI GenBank, AY659991.1) and RNA spike-in control sequences (ERCC, Ambion; for quality control) were included as well.