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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Titration of progeny viruses from all HEK293 cells was done in Vero-E6 cells cultured in 16-well chambered slides (Thermo Fisher Scientific), as described below ...
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bioRxiv - Genetics 2022Quote: ... These plasmids were inserted into the same place in the genome using the Flp-In recombinase technology to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in a monolayer on 10 cm culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (ThermoFisher Scientific, Waltham, MA) supplemented with fetal bovine serum (10% v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
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bioRxiv - Systems Biology 2021Quote: HEK293 cells were maintained at 37°C and 5% CO2 in phenol red-free Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal calf serum (Sigma-Aldrich (MilliporSigma)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were made by co-transfecting plasmids with lentiviral packaging vectors (VSVG and SPAX2) into packaging cells (HEK293-FT) using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants were collected ...
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bioRxiv - Biophysics 2021Quote: The HEK293 cell line subclone TS201A was a gift from Vladimir Yarov-Yarovoy and was maintained in DMEM (Gibco Cat# 11995-065) with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Genetics 2022Quote: ... pgLAP1-3MYC-BioID2 subnit plasmids were then transformed and stably integrated into HEK293-Flp-In-T-REx cells using the manufacturer’s protocol (ThermoFisher Scientific, catalogue #R78007).
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bioRxiv - Cell Biology 2024Quote: Measurement of extracellular Ca2+ clearance by mitochondria from digitonin-permeabilized HEK293 cells was performed using the membrane impermeable Ca2+ indicator Calcium-Green-5N (Life technologies, C3737). Cells were harvested at a density of 500000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.2/NaOH) ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Cell Biology 2022Quote: ... into the Flp-In™ T-REx™ HEK293 cell line or Jaw1 KO cells (#R78007; Thermo Fisher Scientific, Waltham, MA, USA). After incubation for 24 h ...
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bioRxiv - Physiology 2023Quote: ... mVenus:FL_ Proline and mVenus:2x linker proteins were transfected into HEK293 (Catalog CRL-1573, ATCC) cells with lipofectamine 3000 transfection reagent (Catalog L3000008, Thermo Fisher Scientific). 24h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Expression vectors of SLC22A10 orthologs were introduced into HEK293 Flp-In cells either through transient transfection or stable transfection using Lipofectamine LTX (Thermo Fisher Scientific). For transfections in a 48-well plate (seeding density ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into HEK293 cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: S1PR1-Flag and CD69-StrepII were separately expressed using baculovirus-mediated transduction of mammalian HEK293S GnTI− cells (ATCC CRL-3022) in a medium containing FreeStyle 293 (Gibco Cat# 12338018) supplemented with 2% charcoal-dextran stripped fetal bovine serum (Gibco Cat# 12676029) ...
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bioRxiv - Genetics 2023Quote: ... Lentiviruses were produced transfecting HEK293 cells growing on a T75 flask with 20ug of total DNA and Lipofectamine 2000 (Thermo Fisher Scientific) for 16h ...
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Spartin plasmid together with psPAX2 and VSV-G plasmid were transfected into HEK293 cells using Lipofectamine 3000 (L3000015; Thermo Fisher Scientific) for generating lentivirus ...
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bioRxiv - Cell Biology 2024Quote: ... was added for some experiments. HEK293 cells (a gift from H. Choe) used for generation of lentiviral particles were maintained in DMEM (Gibco #11995-065) supplemented with 10% FBS and antibiotic–antimycotic reagent (Gibco #15240-062 ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells (derived from HEK293 cells) were grown in suspension and shaken at 125 rpm using serum-free Expi293TM expression medium (Gibco, Cat#A1435101) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of HeLa and HEK293 cells with siRNAs (100 pmol each) was performed using Lipofectamine RNAi max (Invitrogen-Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... The cells were further incubated for 10 min in Alexa Fluor 488-conjugated donkey anti-sheep LgG (H+L) antibody (ThermoFisher Scientific, A-11015), washed and resuspended in sorting buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... peroxidase-linked species-specific whole antibody (from sheep) secondary antibody (45-000-679, 1:5000-50000 depending on the primary antibody) were both from Fisher Scientific (Waltham, MA). Immunoblots were imaged using Immobilon Western Chemiluminescent HRP Substrate (ECL ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were again centrifuged at 250 rcf for 5 minutes at 4°C and subsequently incubated with matching secondary antibodies (1:200, donkey anti-sheep Alexa Fluor™ 647, ThermoFisher, #A-21448) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 2012) (a gift from Andrew Jackson) at 1:300 and rabbit anti sheep IgG (H+L) secondary link antibody (Invitrogen #31240 1:4000) following Leica BondRx standard IHC protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:100 in blocking solution was followed by a 1-hour incubation with Alexa Fluor 568 Donkey anti-Sheep IgG (H+L) secondary antibody (Invitrogen, A-21099, RRID:AB_2535753) diluted 1:400 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... or HRP-conjugated secondary antibodies (Chicken anti Mouse secondary antibody HRP, Fisher Scientific # A15981, Sheep anti Rabbit secondary antibody HRP, Fisher Scientific #A16172) at RT for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 5% (v/v) defibrinated sheep blood under anaerobic conditions established by placing Anaerogen™ bags in anaerobic 3.5 litre flasks (Oxoid; Thermo Fisher Scientific, Inc.) or in anaerobic flasks (Schuett-Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... Brain slices were blocked with 5% donkey serum in 0.3% PBS-T for 1 h at RT and then incubated with a sheep anti-GFRAL antibody (1:500, Thermofisher, Cat. PA5-47769) and/or a rabbit anti-c-Fos antibody (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... 4 μL from the 100-μL cDNA samples were subjected to RT-qPCR with appropriate primer sets shown in Table S1 and Fast SYBR Green Master Mix (Thermo Fisher Scientific, USA) using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies used in all experiments except Figure S1 and Figure S6 were anti-mouse-Cy5 (1:250; Life Technologies A-10524) and anti-rabbit-Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... and quantitative PCR was performed with primers specified in Table S1 and Fast Taqman 2X mastermix (Applied Biosystems/Life Technologies, Grand Island, NY) on a 7500 Fast Real-time PCR system (Applied Biosystems/Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... and quantitative PCR was performed with primers specified in Table S1 and Fast Taqman 2X mastermix (Applied Biosystems/Life Technologies, Grand Island, NY) on a 7500 Fast Real-time PCR system (Applied Biosystems/Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mutant TDP-43 and 1 mutant C9ORF72 ALS patient were obtained by reprogramming fibroblasts (Additional file 1: Table S1) with CytoTune-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific, Waltham, MA) and differentiated into neurons ...
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bioRxiv - Molecular Biology 2022Quote: ... were PCR amplified from genomic DNA (Pf3D7) using gene-specific primer pairs (Table S1) with Phusion™ High-Fidelity DNA Polymerase (Thermo Scientific, US). The amplified DNA fragment was purified with QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Following overnight incubation with the immunoprecipitating antibody (Supplementary Table S1) or the IgG isotype control (Rabbit Isotype Control (Thermofisher, Cat. No 10500C or Mouse Isotype Control (Thermofisher, Cat. No 10400C)) and 4h of incubation with Pierce™ Protein A/G Magnetic Beads (Thermofisher ...
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bioRxiv - Cancer Biology 2021Quote: ... The precleared lysates were then incubated at 40C overnight with the immunoprecipitating antibody (Supplementary Table S1) or with the IgG isotype control {Rabbit Isotype Control (Thermofisher, Cat. No 10500C or Mouse Isotype Control (Thermofisher, Cat. No 10400C) and for 4 additional hours with Pierce™ Protein A/G Magnetic Beads (Thermofisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies used in this study were listed in Table S1 and secondary antibodies fused to Alexa fluorophore (Molecular Probes, 1:500). All stained sections were analysed with Zeis Imager Z2 (Zeis ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Molecular Biology 2021Quote: ... Successful expression of the respective SARS-COV-2 ORF mRNA transcripts was measured by reverse transcription PCR using gene-specific primers (Table S1) and SuperScript III One-Step RT-PCR system (Invitrogen; Cat#: 12574-030). To measure SARS-COV-2 gene-specific activities ...
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bioRxiv - Microbiology 2021Quote: ... The presence of recombinant virus was verified by immunostaining with monoclonal antibody against the nucleoprotein (FujiRebio, Cat# 800-092) and polyclonal antiserum against the S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate VERO cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ13 catalytic domain was amplified with the primers listed in Table S1 and cloned into SunTag dCas9 plasmid using the BsiWI restriction enzyme (Thermo Scientific™, ER0851). The final construct allows to produce (i ...
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bioRxiv - Neuroscience 2024Quote: ... was generated with each of the 47 TaqMan probes (S1 Table 5) and Xeno primer (TaqMan Cells-to-Ct Control kit, Thermo Fisher Scientific, Sweden) and using the Taq-SSIII reaction mix (CellsDirectTM One-Step qRT-PCR Kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 14 transcripts of chitin biosynthesis signaling genes were prepared in vitro using primers ligated with T7 RNA polymerase promoter sequences (Table S1) using MEGAscript™ RNAi kit (AM1626, Ambion, California, USA). dsRNAs were further purified with MEGAclear columns (Ambion ...
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bioRxiv - Cell Biology 2023Quote: RBM12 CRISPR knockout HEK293 clones were generated by transfecting wild-type cells with two independent gRNAs (Table S1) using Lipofectamine-2000 transfection reagent (Thermo Fisher, Cat. #11668027) following recommended protocols ...