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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The peptides from each sample were labeled with individual Tandem Mass Tags (TMT) (Thermo Fisher Scientific) as previously described50 ...
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bioRxiv - Microbiology 2024Quote: ... Phosphorylation efficiency was analyzed on a 12% acrylamide Phos-Tag gel stained with SYPRO Orange (Invitrogen) following manufacturer’s instructions and visualized on a Chemi-Doc MP (BioRad).
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bioRxiv - Molecular Biology 2024Quote: ... Tissues from Knock-in LAP-tagged PAF1C flies were detected with anti-V5 tag (Invitrogen, R96025) 1:500 in BBX incubated overnight at 4°C with nutation ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Labeling: This experiment required two Tandem Mass Tag (TMT) 16plexes (Thermo Scientific, A44520; lot number WJ327115). The labeling schemes for multiplex experiment are included in the Supplemental Material ...
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bioRxiv - Biochemistry 2020Quote: MBP-tagged (pTHMT) full-length SARS-CoV2 nucleocapsid protein was expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7 to 0.9 before induction with 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2020Quote: ... Biotin signal was visualized using a Chemiluminescent Nucleic Acid Detection Module kit (Thermo Scientific, Cat. #89880). Competition experiments were performed using from 100- or 1000-fold levels of unlabeled fragments.
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bioRxiv - Cell Biology 2020Quote: ... and labelled with a 3-fold molar excess of EZ-Link NHS LC LC Biotin (ThermoFisher) for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... or biotin-XX conjugated goat anti-mouse IgG1 (4 μg/ml, Invitrogen; Therrmo Fisher Scientific, Inc.) were added to their respective plates and incubated at room temperature for 2 h ...
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bioRxiv - Immunology 2021Quote: ... was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific, Inc.) and labelled with Cy3 monoreactive dye pack (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... or biotin-XX conjugated goat anti-mouse IgG1 (4 μg/ml, Invitrogen; Therrmo Fisher Scientific, Inc.) were added to their respective plates and incubated at room temperature for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylation of the 4sU/4tU labelled RNA was performed with EZ-Link HPDP-Biotin (Thermo Fisher) for 30 minutes at 65°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The probe was labeled with biotin using T7 RNA Polymerase-Plus Enzyme Mix (Thermo Scientific, USA) by the addition of biotinlabeled UTP according to the manufacturer’s protocol.
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bioRxiv - Genomics 2020Quote: ... and the biotin-labeled DNA fragments were pulled by the streptavidin coated Dynabeads M280 (Thermo Fisher). Then discard the supernatant and add 78 μl H2O ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: ... and incubated with 1 mg/ml of EZ-Link Sulfo-NHS-SS-Biotin (21331, Thermo scientific) for 30 min ...
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bioRxiv - Physiology 2019Quote: ... 7 mg of protein were incubated with 343 μM Maleimide-PEG2-biotin (Thermo Fisher Scientific, #21901BID) at room temperature for 30 minutes with agitation by precipitating the alkylated protein with 1 mL of 100% cold acetone by incubating at −20°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... treatment and for the presence of the Biotin marker by Dynabeads MyOne Streptavidin C1 beads (Invitrogen) pull-down ...
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bioRxiv - Immunology 2021Quote: ... coupled with 0.1µg/µl CCP2-biotin followed by IgG capturing using FcXL affinity beads (Thermo Scientific). N-linked glycans were released using PNGaseF ...
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bioRxiv - Microbiology 2021Quote: ... with a 100-fold molar excess of EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Fisher). Biotinylated oligonucleotides were extracted from a 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM MgCl2) before biotinylation using EZ-Link NHS-PEG4-biotin (Thermo Fisher Scientific, Waltham, Massachusetts) in equimolar concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... with one primer containing a biotin residue for linkage to magnetic beads (Invitrogen MyOne Strep Dynabeads). Immobilized templates were incubated with yeast nuclear extracts ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotin pulldown of the ligation junctions: 15 μl of MyOne Dynabeads Streptavidin C1 (Invitrogen; Cat#: 65001) beads washed with the TWB buffer (5 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2020Quote: ... by adding the following components to the reaction: 37.5µl of 0.4mM biotin-14-dATP (Life Technologies), 1.5µl of 10mM dCTP ...
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bioRxiv - Biochemistry 2021Quote: NPF (WAVE(PVCA) WH2) molecules were labeled with EZ-Link maleimide-PEG4-Biotin (Thermo Fisher Scientific) at the C-terminally located KCK ...
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bioRxiv - Bioengineering 2021Quote: ... IgM or C1q samples were biotinylated using NHS-PEG4-biotin EZ-link kit (Thermo Fisher Scientific) and manufacturer conditions ...
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bioRxiv - Cell Biology 2021Quote: ... and surface-labeled at 4 °C with 0,2 mg/ml sulfo-NHS-SS-biotin (Thermo Scientific) in PBS1X for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... GP was directly labeled using the amine-specific biotinylation reagent EZ-Link NHS-PEG4-Biotin (ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... purified 40S-RACK1-ybbR subunits were incubated with 4-fold molar excess PEG11-biotin (ThermoFisher #21911) conjugated to co-enzyme A (CoA ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin signal on nylon membrane was detected using a streptavidin-based chemiluminescent method (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 100 μl of freshly prepared 25 mM EZ-Link™ Iodoacetyl-PEG2-Biotin (IPB, Thermo Scientific) was added to the solubilized cell particles to a final concentration of 5 mM and incubated at RT for 30 min in the darkness ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... The pull-down of biotin adapter-containing DNA was performed using MyOne C1 Beads (ThermoFisher, 65001) for 30 minutes at room temperature with rotation ...
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bioRxiv - Immunology 2022Quote: ... Thioredoxin-fused peptides were chemically biotinylated with EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and purified anti-IFNγ and BsAb were biotinylated by EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher) and buffer exchanged by Zeba Spin Desalting Columns (7K MWCO ...
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bioRxiv - Cancer Biology 2022Quote: ... the αCD3 scFv monomer was labeled with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher, 21335) according to the manufacturer’s protocol and excess NHS-biotin was removed with 0.5 mL Zeba Spin Desalting Columns (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Nascent proteins were labeled with biotin using the Click-it protein reaction buffer kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... surface receptors were labeled with Pierce™ Premium Grade Sulfo NHS-SS-Biotin (Thermofisher, Waltham, USA) and purified using Streptavidin High Performance Spintrap™ (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... digoxigenin- and biotin-labeled sense and anti-sense primers (Integrated DNA Technologies, Coralville, IA, or Invitrogen, Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were dialyzed into bicarbonate buffer and biotinylated using EZ-LinkTM Sulfo-NHS-LC-Biotin (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant BspC was biotinylated using a EZ-Link Sulfo-NHS-Biotin kit (ThermoFisher Scientific, Waltham, MA). The PVDF membrane was probed with the biotinylated BspC (100µg ...
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bioRxiv - Molecular Biology 2019Quote: ... monodispersed (>95% viability) and then labeled with EZ-link-sulfo-NHS-LC-biotin (Thermo Fisher Scientific) in solution essentially as described (Tseng et al. ...
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bioRxiv - Genetics 2020Quote: ... and Southern blots with the North2South Biotin Random Prime Labeling and Detection Kit (ThermoFisher cat. #17175) and the wild type hH2Az gene fragment used as a probe.
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bioRxiv - Developmental Biology 2020Quote: ... Synthesis of double-stranded cDNA and biotin-labelled cRNA was performed according to manufacturer’s instructions (Affymetrix). Fragmented cRNA preparations were hybridized to Drosophila genome oligonucleotide arrays (GeneChip Drosophila Genome 2.0 Array (3’ IVT Expression Affymetrix)] using an Affymetrix hybridization Oven 640 ...
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bioRxiv - Cell Biology 2019Quote: ... Hoechst was used to visualise DNA and biotin with AlexaFluor®568-conjugated streptavidin (Molecular probes). Images were acquired on a Zeiss Axioimager M1 ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1, 0.25mM dCTP, 0.25mM dGTP, 0.25mM dTTP, 0.25mM biotin-dATP (ThermoFisher#19524016), 50U Klenow polymerase Polymerase I (NEB M0210L)) ...
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bioRxiv - Genetics 2021Quote: ... Biotin-labelled probes were detected with Streptavidin-Alexa 660 (Thermo Fisher Scientific, Waltham, MA, United States); digoxigenin-labelled probes were detected with anti-digoxigenin-FITC (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... the slice storage solution was supplemented with 50 μM Sulfo-NHS EZ Link Biotin (Thermo Fisher) for 45 min before washing and further storage ...
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bioRxiv - Immunology 2021Quote: ... Purified ACE2 protein was biotinylated using the EZ-Link Sulfo-NHS-Biotin kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: Old yeast cells were isolated by using EZ Link Sulfo-NHL-LC-LC-Biotin (Thermo Scientific) to label cell surface proteins (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific] ...
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bioRxiv - Cell Biology 2022Quote: ... Sulfo-NHS-LC-LC-Biotin (Pierce) and Neutravidin agarose beads (Pierce) from Fisher Scientific (Waltham, MA). Culture media (Dulbecco’s Modified Eagle’s Media [DMEM] with 4.50 g/L glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... Click-iT reaction cocktail with biotin-conjugated azide was prepared according to the manufacturer’s recommendations (Invitrogen), and cells were incubated for 80 min ...