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Citations for Thermo Fisher :
4751 - 4800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... 3 µL of diluted cDNA diluted was added to 1 µL of each primer (S1 Table) with 10 µM and 5 µL of SYBR Green Master Mix (Applied Biosystems, Life Technologies), using the following thermal program ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Immunology 2022Quote: RBD and Fab fragment from NCV2SG48 with 6xHis-tag expressed in Expi293F cells (Thermo Fisher Scientific) were purified using Ni-NTA Agarose resin (QIAGEN) ...
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bioRxiv - Plant Biology 2019Quote: ... or pDEST-24 carrying a C-terminal GST purification tag (Thermo Fisher Scientific, Hampton, NH, USA).
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bioRxiv - Cancer Biology 2019Quote: ... The Alexa Fluor 488 - labeled RNA probe was obtained using the FISH Tag RNA Kit (Invitrogen). In the first step ...
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bioRxiv - Microbiology 2021Quote: ... DNA FISH probes were prepared using the FISH-Tag DNA Multicolor Kit labeling kit (Life Technologies) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... were labelled with a different TMT tag (20 mg mb1 in 40 ml acetonitrile) (Thermo Scientific), for 2 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™ ...
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bioRxiv - Cell Biology 2021Quote: ... labeled with Tandem Mass Tag (TMT) 11plex labeling reagent kit following manufacturer’s instructions (Thermo Fisher Scientific), quenched with 5% hydroxylamine ...
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bioRxiv - Systems Biology 2021Quote: ... The protein digests were labeled with 10-plex Tandem Mass Tag (TMT) reagents (Thermo Fisher Scientific) (Dayon et al. ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... the N-terminal His6-tag was removed by the addition of His6-rTEV protease (Life Technologies) and dialyzed in H Buffer for 36 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting peptides were labeled by 16-plex TMTpro-tags (TMTpro, Thermo Fisher Scientific, Waltham, MA, USA) using the same amount of respective label for each sample ...
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bioRxiv - Biochemistry 2022Quote: ... HA-KRASG12C was immunoprecipitated using the Pierce HA-Tag IP Kit (26180, Thermo Scientific, Waltham, MA) and separated by SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... Then Flag tag was captured with Pierce Protein A/G Agarose (20 μL per sample, ThermoFisher) at room temperature for 2 h with mixing constantly ...
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bioRxiv - Neuroscience 2022Quote: Antibodies used for these experiments include: anti-HA-tag (Mouse, ProteinTech, Thermo Fisher, 50-173-6449), phospho-TAOK2 (S181 ...
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bioRxiv - Cell Biology 2022Quote: ... mESC for omni-ATAC-seq and CUT&Tag were fixed in 1% formaldehyde (Thermo Fisher SCIENTIFIC) and quenched by adding glycine (final concentration 0.125M ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged ChElp2 was pulled-down with Dynabeads™ His-Tag (Thermo Fisher Scientific, Massachusetts, USA) and the subsequent western blot analyses were performed with anti-His (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were analyzed by SDS-PAGE and western blot using DYKDDDDK Tag Monoclonal Antibody (FG4R) (Invitrogen) (1 ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... Total cysteine content was labeled with tandem mass tag (TMT) iodoTMTsixplex™ (90102 Thermo Fisher Scientific) followed by quenching with 20mM 1DTT (43816 Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for Cut&Run: mouse monoclonal anti-V5 tag (ThermoFisher, # R960-25) diluted to 0.01 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Tryptic peptides were labeled using a tandem mass tag 10-plex isobaric label reagent set (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: Tryptic peptides were labeled with isobaric tandem mass tags (TMT10plex or TMTpro 16plex, Thermo Fisher Scientific). Peptides were resuspended in labeling buffer (2 M urea ...
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bioRxiv - Cancer Biology 2020Quote: ... 340 µg of peptides were labeled with 10-plex Tandem Mass Tag reagents (TMT10, Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Molecular Biology 2021Quote: Mimivirus L375 appended with a C-terminal 10X histidine tag was generated by GeneArt (Life Technologies) for codon-optimized expression in Escherichia coli ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Cell Biology 2021Quote: ... Nb48 sequence was cloned into the pcDNA3.1 C-terminus tag 3HA vector (derived from pCDNA3.1 Invitrogen) between NheI-XhoI restriction sites and expressed transiently as an intrabody ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-HA-Tag (16B12) Alexa flour-594 (Invitrogen, A21288, Lot: 2218838, IF: 1:100); mouse monoclonal anti-FLAG M2 (Sigma ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Cancer Biology 2023Quote: ... digested peptides were differentially labelled using Tandem Mass Tag (TMT) 16-plex reagent kit (Thermo Scientific) following manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... and inserted into a pET-SUMO expression vector containing an N-terminal hexahistidine-SUMO tag (Invitrogen). PARP1 truncations (PARP1-ZnF ...
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bioRxiv - Neuroscience 2024Quote: ... extracted protein was immunoprecipitated using 25μL prewashed HA Ab-Tag Magnetic beads (Thermo Fisher Scientific, Massachusetts) with gentle rotation at 4°C for 30min ...
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bioRxiv - Microbiology 2024Quote: ... anti-KSHV ORF45 (MA5-14769) and anti-STREP-TAG II (MA5-37747) were obtained from Invitrogen. The anti-KSHV ORF57-HRP (sc-135746) ...
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bioRxiv - Immunology 2024Quote: For SULFO-TAG-labeling of the Ub detecting antibody (ThermoFisher #14-6078-37, Ub (clone P4D1)) ...
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bioRxiv - Neuroscience 2023Quote: Co-immunoprecipitation was performed using the Pierce Magnetic HA tag IP/Co-IP Kit (ThermoFisher, #88838). The manufacturer’s Manual IP/Co-IP protocol and the Elution Protocol using sample buffer were applied.
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bioRxiv - Immunology 2023Quote: ... Produced nanobodies were purified from yeast supernatants using a CaptureSelect C-tag column (Thermo Fisher Scientific) and an Äkta Start (Cytiva) ...
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bioRxiv - Plant Biology 2023Quote: ... Triacylglycerol (TAG) levels were estimated by a fluorometric assay using the dye Nile Red (Thermo Fisher)(Yu et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... The HA tag was inserted after the signal sequence of Fzd6 in the pCR2.1-TOPO (Invitrogen) vector by In-Fusion cloning ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... of ANGPTL8 with Myc-DDK tags were used for transfection using Lipofectamine 3000 (Invitrogen, Carlsbad, CA) with 5ug of plasmid for 48hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic peptides were labeled using a tandem mass tag 10-plex isobaric label reagent set (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The remaining TMT tags were quenched by adding 100 mM tris buffer (pH 8.0; Thermo Scientific) and incubating for over 5 min at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... Peptides were then labeled with TMT using a TMT10plex mass tag labeling kit (Thermo Fisher Scientific) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were labeled using 100 ug of 16-plex tandem mass tag (TMTpro) reagents (Thermo Fisher), with incubation for 60 min at 22 °C ...
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bioRxiv - Microbiology 2023Quote: ... Samples were labeled using Tandem Mass Tag (TMT) 16plex reagents (Thermo Scientific, A44520; lot number XA341491) following manufacturer protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Potential candidates were investigated by western blotting against the integrated V5-tag (Thermo Fisher, R960-25) with or without 500 uM 3-indoleacetic acid (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide samples were then reacted with tandem mass tag (TMT) 16/18-plex reagents (Thermo Fisher) in 40% vol/vol acetonitrile and incubated for 1 hour at room temperature ...