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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Whole transcriptome RNA-Seq (wtRNA-Seq) libraries were prepared from total RNA (5 μg per sample) plus ERCC RNA Spike-In (Ambion® by Life Technologies, 4456739) followed by selective ribosomal RNA (rRNA ...
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bioRxiv - Cell Biology 2022Quote: The HEK293 cells were grown in DMEM containing 10% FBS and transfected by pcDNA-Spike or pcDNA vector using lipofectamine 300 reagent (ThermoFisher, Waltham, MA, USA). Two days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 ng of each RNA sample was supplemented with 1 μL (1:100 dilution) of ERCC spike-in control (Life Technologies, Cat# 4456740). Poly-A RNA enrichment was performed using NEBNext Poly(A ...
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bioRxiv - Pathology 2020Quote: ... Phoenix cells were grown in DMEM containing 10% FBS and co-transfected by pLV-mCherry and pcDNA-Spike or pMD2.G vector using a calcium phosphate kit (ThermoFisher, Waltham, MA, USA). The supernatant with produced virus (Spp or VSV-G lentivirus ...
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bioRxiv - Developmental Biology 2022Quote: ... barcode-indexed RNA sequencing (RNA-seq) deep-sequencing libraries were synthesized from total RNA with ERCC spike-in controls (Thermo Fisher Scientific, MA) using Ovation RNA-Seq Systems 1-16 for Mouse (NuGen Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... A 500 ng quantity of RNA was used to prepare RNA-seq libraries and spiked-in with ERCC ExFold Spike-In mixes (Thermo Fisher Scientific, 4456739). RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina ...
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bioRxiv - Immunology 2024Quote: ... The final spike protein concentration was determined by measuring absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific™). Proteins were dispensed into 0.5 ml aliquots and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were constructed from cDNA using the Ion Total RNA-seq Kit v2 with ERCC RNA Spike-in Control mix (Thermo Fisher, Waltham, USA). 25μL of each library was sequenced using a PI V2 chip in an Ion Proton Sequencer (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: All strains were cultured for 16-20 hours (B. anthracis) or 24 hours (Y. pestis) on BD BBLTM Trypticase Soy Agar II with 5% sheep blood (SBA) (Thermo Fisher Scientific, Waltham, MA, USA) at 35°C in ambient air from glycerol stocks stored at −170°C.
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bioRxiv - Microbiology 2019Quote: The 51 Salmonella isolates were struck for isolation from the frozen stocks to Tryptic Soy Agar (TSA) with 5% sheep blood (BAP) (Thermo Fisher Scientific Inc, Waltham, MA) and incubated overnight at 37°C to ensure purity ...
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bioRxiv - Cell Biology 2019Quote: ... 18 and 19 or TSS were pre-cleared by incubation with 1 × 107 Dynabeads magnetic beads conjugated to sheep α-mouse IgG (Invitrogen Dynal AS, Oslo Norway) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... and the deletion mutant 04-309 ΔpamZ were cultivated as follows: bacteria were grown on Columbia sheep blood agar (CSA, Thermo Fisher Scientific Oxoid, Schwerte, Germany) medium plates at 37°C for 2-3 days ...
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bioRxiv - Neuroscience 2023Quote: ... was analyzed by indirect immunofluorescence staining using sheep anti-AnkB (Qu F et al. 2016) and AlexaFluor secondary antibodies (Thermofisher #A-11039, RRID: AB-2534096). Stimulated emission depletion microscopy (STED ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction obtained by centrifugation at 15,000 rpm for 15 min was reacted with sheep anti-mouse or anti-rabbit primary antibody-conjugated Dynabeads M280 (Thermo Fisher Scientific, Waltham, MA, USA) for 4 h or overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... Cells were cultured in DMEM (Wisent) or RPMI1640 supplemented with 10% FCS and penicillin/streptomycin (Gibco). Media were supplemented with 200 UI/mL IL-2 (Proleukin – Novartis Pharmaceuticals ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% (v/v) heat-inactivated Hyclone fetal calf serum (FCS; Thermo Scientific, UT, USA), 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... Nanobody-Fc fusion supernatants were passed over a column with 4 mL protein G resin (ThermoFisher), which was then washed with 40 mL of HBS ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cells were then subjected to Fc blockade with anti-CD16/CD32 (Thermo Fisher Scientific, Waltham, MA), washed with 1XPBS ...
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bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... competition was detected by HRP-goat anti-human IgG Fc secondary antibody (A18817, Thermo Fisher Scientific). The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... explanted organs were stored in 600 μL DMEM containing 10% FCS and 1% Penicillin/Streptomycin (Gibco). After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II ...
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bioRxiv - Cancer Biology 2024Quote: ... KELLY cells were cultured in RPMI medium with 10-15% fetal calf serum (FCS) (Life Technologies). Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The IgGs were purified by adding Protein G (Cytiva) or CaptureSelect IgG-Fc resin (Thermo Scientific) to the supernatant and rolling overnight at 4°C ...