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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The sequence encoding mouse MmCyp1a2 and maize ZmCYP81A9 were codon-optimised for in planta expression and synthesized as a GeneString (GeneArt, ThermoFisher Scientific, Supplemental Table S1). Using BpiI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... ssRNA/dsRNA fractionation was performed as previously described 48. To visualize dsRNA, 1 µg were pretreated with RNase-free DNase1 (RBC Bioscience, cat. #9680DN050) and S1 nuclease (Thermo Scientific™, cat. #EN0321) according to the manufacturer’s instructions and electrophoresed on 1% agarose gel stained with ethidium bromide.
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bioRxiv - Cancer Biology 2023Quote: ... About 10-100 ng genomic DNA with 2.5 μM primers targeting the promoter region of PTEN or ATAD1 and a genomic region on Chr 12 as an internal control (Supplementary Table S1) and PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™) were mixed ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were incubated with HRP-conjugated secondary antibodies at 1:10000 for 1h at room temperature (see Table S1 for suppliers) and developed with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher®, Waltham, US) on CL-X Posure™ X-ray films (34091 ...
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bioRxiv - Molecular Biology 2023Quote: ... The rfc3 gene was amplified from Xenopus egg cDNA by two-step PCR using primers listed in Supporting Table S1 and cloned into pDONR201 (Thermo Fisher Scientific, MA, USA) by the Gateway BP reaction ...
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bioRxiv - Genomics 2023Quote: Lipofectamine 3000-DNA transfection complexes were prepared by diluting 10ug of pHAGE-EF1a-BRD4-NUT DNA (Datafile S1) or pUC19 (negative control) in 500ul of DMEM/F12 media (ThermoFisher, catalog no. 11330-032), mixing well and incubating for 1 min ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... cRNA was prepared according to the manufacturer’s instructions from linearized plasmids encoding SARS-COV2 Protein E using the mMESSAGE mMACHINE T7 kit (Ambion, TX, USA). RNA concentrations were quantified by UV spectroscopy (NanoDrop ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-afadin (clone D1Y3Z) or Alexa Fluor 488 anti-DYKDDDDK (flag) tag monoclonal antibody (L5, Life Technologies). The plasma membrane was stained with CF594 dye conjugated wheat germ agglutinin (Biotium) ...
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bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the following primary/secondary antibodies: rabbit anti-6x-His Tag (ThermoFisher Scientific PA1-983B, 1:10,000), mouse monoclonal anti-GFP (Abcam ab1218 ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were immunoprecipitated by incubating with an antibody to the c-Myc tag (Invitrogen 13-2500) on BBS2 as described above and immunoblotted with the c-Myc antibody for BBS2 and with the anti-Flag antibody (Sigma F3165 ...
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bioRxiv - Neuroscience 2020Quote: ... Tandem Mass Tags (TMT 10-plex) kits and microBCA protein assay kit were purchased from Thermo Fisher Scientific (Rockford ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained with anti-GFP antibody (5 μg/ml, Thermo Fisher, RFP Tag Monoclonal Antibody RF5R) and whole node confocal scans were used to quantify percent metastatic area of total node via image thresholding in ImageJ ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Biophysics 2020Quote: ... the kdpFABC operon with the 8x histidine tag was cloned into the pBAD vector (Invitrogen, Carlsbad CA) and introduced into E ...
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bioRxiv - Cancer Biology 2020Quote: ... Pierce Trypsin/Lys-C Protease Mix and tandem mass tag (TMT)-labeling kit were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via affinity pulldown of their StrepII tags using a StrepTrap HP column (Fisher Scientific) and eluted into Buffer A supplemented with 2.5mM desthiobiotin (Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Ribosome nascent chain complexes (RNCs) were purified via the encoded His-Tag using magnetic Dynabeads (Invitrogen, #10104D). The translation reaction was applied to equilibrated beads (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tandem Mass Tag™ 6-plex (TMTsixplex™) reagents kits for isotopic labeling were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... Desalted peptide samples from each sample were labeled with 10-plex tandem mass tags (TMT, ThermoFisher Scientific) with one of the TMT channels (131 ...
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bioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) eleven plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Physiology 2022Quote: ... 25 μg of peptides per sample were labeled with isobaric tandem mass tags (TMT10plex, Thermo Fisher Scientific) as described previously [48] ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen), diluted to 1:20 000 in 5 % (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and delivered into a modified pEG containing 3XFLAG and 9XMYC tags using the LR reaction kit (Invitrogen). The pEG destination vectors were transformed into Agrobacterium strain AGL0 ...
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bioRxiv - Molecular Biology 2020Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and pooled ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Systems Biology 2020Quote: ... 250 μg of peptide were labeled using TMT11Plex Mass Tag Labeling Kit (Thermo Scientific, Lot number UE283355) following the manufacturer’s instructions and they were combined as shown in Figure 1 ...
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bioRxiv - Biochemistry 2021Quote: ... Desalted peptides were labeled using a Tandem Mass Tag 11-plex isobaric label reagent set (ThermoFisher #A34808) with the following setup ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Immunology 2020Quote: ... the polyhistidine tag of 6xHis-TEV-dreC5a was removed by incubation with TEV protease (Thermo Fisher Scientific) and the protein was FITC-labelled at the N-terminus using the sortagging method (37) ...
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bioRxiv - Microbiology 2019Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled ...
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bioRxiv - Physiology 2020Quote: ... 25 μg of peptides were labeled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific) as described previously (34) ...
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bioRxiv - Biochemistry 2021Quote: ... as described in (62).The 6xHA with linker and mCitrine tags were cloned into pDONR-P2RP3 (Invitrogen) as described in de Saint Germain et al ...
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bioRxiv - Cell Biology 2021Quote: The LAP tag from pIC113 vector (Cheeseman and Desai, 2005) was subcloned into pcDNA5.0/FRT vector (Invitrogen) to make the pcDNA5.0/FRT-LAP-N vector ...
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bioRxiv - Biochemistry 2021Quote: ... followed by a short linker FLAG-epitope tag and C-terminal Histidine6 was synthesised by GeneArt (Invitrogen). To generate a FLAG-free HuACE2 fusion ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... labelled with Tandem Mass Tag (TMT) 6-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptide mixtures were labelled with isobaric TMT-tags according to the manufacturer’s protocol (Thermo Scientific). Online LC-MS was performed using a Dionex UltiMate™ 3000 RSLCnano System coupled to a Q-Exactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (ThermoFisher Scientific, Inc) and the labelled samples pooled.
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bioRxiv - Neuroscience 2022Quote: ... with 0.5 mg of Tandem Mass Tag Pro (TMTpro) reagent (16-plex kit, manufactures instructions Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Systems Biology 2024Quote: ... The protocol was performed as in (Ref.16) with a V5-tag antibody (Thermo Fisher, R960-25) that was added to a final dilution of 1:100.
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bioRxiv - Microbiology 2024Quote: ... PCR amplification was subsequently performed using Platinum Tag DNA Polymerase High Fidelity (ThermoFisher Scientific, Cat. #: 11304-102). Amplicons of the same samples were pooled together before purification ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... Eluted peptides were vacuum dried and tandem mass tags labeling was performed (11-plex TMT, Thermo Scientific) by following manufacturer instructions ...