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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... as per the manufacturer’s protocol) and a fluorescent Alexa Fluor tag (Alexa Fluor® succinimidyl esters, Invitrogen™ ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Cell Biology 2023Quote: ... CSS1 and CSS2 were synthesised together with a V5-tag using GeneArt DNA Synthesis (Thermo Fisher Scientific) and then cloned into pEU-Flexi ...
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bioRxiv - Biochemistry 2023Quote: WDR5 and WDR5 N225A were cloned into the pEF-dest51 destination vector with a V5 tag (Invitrogen) resulting in the expression clones pEF-dest51-WDR5 and pEF-dest51-WDR5-N225A ...
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bioRxiv - Biochemistry 2023Quote: ... The His tag was digested during 14h at 4°C with histidine-tagged 3C proteases (Thermo Scientific) according to the manufacturer’s recommendations ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... resuspended in 200 mM HEPES and incubated with 0.1 mg Tandem Mass Tag (TMTzeroTM, Thermo Fisher Scientific) label reagent for 2h at 26°C and 450 rpm ...
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bioRxiv - Cell Biology 2023Quote: A cDNA encoding p38α was subcloned into pcDNA6 with an N-terminal myc tag (Invitrogen, Carlsbad, CA), and a kinase-dead (D168A ...
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bioRxiv - Cell Biology 2023Quote: ... Tandem mass tag labeling (TMT) was performed using the TMT 10-plex reagent kit (ThermoFisher, Waltham, MA). Detailed methods of liquid chromatography-mass spectroscopy analysis is included in the Supplemental Material.
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bioRxiv - Cell Biology 2023Quote: ... Proteins of interest were labelled with antisera against the FLAG tag (Thermo Fisher Scientific, Waltham, MA, USA) and ATP5A (Abcam ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cardiomyocytes were labeled with of DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17, Invitrogen, MA1-142-A488) at 1:500 dilution in 1% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... 6X-His Tag Monoclonal Antibody (HIS.H8) and Goat Anti-Mouse IgGH&L (HRP) were preferred (Thermo Scientific, Cat.#MA1-21315 ...
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bioRxiv - Biochemistry 2023Quote: ... Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher, P/N 90061). After labeling ...
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bioRxiv - Neuroscience 2023Quote: ... Correction factors were added to the tags based on the reagent instructions (ThermoFisher, Cat# 90110, Lot: UL291038). In both cases ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling with Tandem Mass Tags (TMT, TMT10plexTM or TMTpro™ 16plex, Thermo Fisher Scientific cat. No 90110) was carried out according to manufacturer’s instruction with the following changes ...
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bioRxiv - Molecular Biology 2023Quote: ... HDR templates containing the FKPB12F36V tag were synthesised (see supplementary table 1) and cloned by GeneArt (Invitrogen) into double-stranded plasmid vectors (pMX).
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bioRxiv - Cancer Biology 2024Quote: ... Tandem Mass Tagging (TMT) labelling was performed using Tandem Mass Tag Reagents kit (Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... The protocol was performed as in (Ref.16) with a V5-tag antibody (Thermo Fisher, R960-25) that was added to a final dilution of 1:100.
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bioRxiv - Cell Biology 2023Quote: ... and subjected to fluorescently-tag secondary antibodies staining (Alexa Fluor 488 or Alexa Fluor 594, Life Technologies). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... stained with primary antibodies against GFP Tag Monoclonal Antibody (3E6) (1:500, mouse, Thermo Fisher Scientific, A11120) and cleaved Caspase-3 (rabbit ...
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bioRxiv - Microbiology 2021Quote: Viral N protein was detected using a rabbit anti-SARS-CoV N antibody [32] as a primary antibody with AlexaFluor 568 anti-rabbit IgG or anti-rabbit IgG-HRP (Thermo Fisher) as secondary antibodies together with DAPI to stain the nucleus by indirect immunofluorescence as described previously [33].
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bioRxiv - Biophysics 2021Quote: ... Alexa Fluor 405 NHS ester (A30000) and EZ-Link NHS-PEG4-Biotin were purchased from Thermo Fisher. ATTO 647N NHS ester (18373-1MG-F) ...
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bioRxiv - Biophysics 2020Quote: Quartz coverslips coated with polyethylene glycol and biotin (MicroSurfaces) were sonicated in Tween-20 (Thermo Fisher Scientific) and extensively washed with ddH2O before binding to silicone hybridization chambers (SecureSeal™ hybridization chambers ...
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bioRxiv - Immunology 2021Quote: ... excess Alexa Fluor 647 and biotin were removed using 7-kDa Zeba desalting columns (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: BG505 SOSIP trimers were randomly biotinylated using the EZ-Link NHS-PEG4-Biotin kit (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then biotinylated using NHS-PEG4-Biotin (125 µM unless otherwise specified, ThermoFisher Cat. No. A39259) diluted in PBSCMA to achieve a total volume of 50 µL per well for 30 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... rapa (Xiong and Pires, 2011) were labelled by random priming with biotin-14-dUTP (Invitrogen, Life Technologies). The ribosomal probe used in this study was pTa-71 (Gerlach and Bedbrook ...
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bioRxiv - Genomics 2020Quote: ... rapa (Xiong and Pires, 2011) were labelled by random priming with biotin-14-dUTP (Invitrogen, Life Technologies). The ribosomal probe used in this study was pTa-71 (Gerlach and Bedbrook ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... After the surface protein was labelled with 1 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific), the cells were lysed in PBS containing protease inhibitor cocktail (consisting of AEBSF ...
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bioRxiv - Cell Biology 2019Quote: Microtubules were polymerised from bovine tubulin with stochastic incorporation of ATTO390 (Attotech, Germany) and biotin (ThermoFisher, #20217) labelled tubulin ...
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bioRxiv - Biophysics 2021Quote: ... the respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was resolved in H2O and directly added to the protein solution in a final molar ratio of 1:1 in 25 mM MES ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg of recombinant 6xHis-4xSUMO2-Strep was biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Plant Biology 2020Quote: ... The DpnII-digested chromatin was end-labeled with biotin-14-dATP (Thermo Fisher Scientific, Waltham, MA, USA) and used for in situ DNA ligation ...
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bioRxiv - Microbiology 2021Quote: ... the RBD was biotinylated using EZ-Link NHS-LC-LC-Biotin (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The equimolar mRNAs were labeled using biotin with an RNA 3’ End Desthiobiotinylation Kit (Thermo scientific, USA). The labeled mRNAs were then captured using streptavidin magnetic beads (Thermo scientific ...
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bioRxiv - Immunology 2022Quote: Recombinant gH/gL proteins were biotinylated using the EZ-Link NHS-PEG4-Biotin Kit (ThermoFisher, catalog #21330) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... bound biotin-MIF was revealed via chemiluminescence using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) on a LAS- 3000 Imaging System (Fuji Photo Film Co. ...
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bioRxiv - Cell Biology 2022Quote: ... we performed the cell-surface biotinylation assay utilising the EZ-Link Sulfo-NHS-SS-Biotin (ThermoFisher Scientific) as per manufactures’ recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding the following components to the reaction: 37.5 µl of 0.4mM biotin-14-dATP (Life Technologies), 1.5 µl of 10mM dCTP ...
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bioRxiv - Microbiology 2020Quote: ... RBD-6H (340-538; NITN.GPKK) was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256; Life Technologies). Serial half-log dilutions (starting at 1 μM ...
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bioRxiv - Immunology 2020Quote: ... CD8α-biotin (53-6.7) Biolegend (Ref 100703) and B220 (RA3-6B2) from Invitrogen (Ref 14-0452-81). Images were collected using a Zeiss LSM 510 confocal microscope ...
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bioRxiv - Neuroscience 2021Quote: ... EZ link sulfo NHS SS Biotin and ProLong™ Gold Antifade Mountant with DAPI (Thermofisher scientific, MA).
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bioRxiv - Bioengineering 2021Quote: ... 3 × 105 EPCs were incubated with 1 µM LXW7-biotin in a binding buffer (1X HEPES (Gibco) containing 10% FBS (HyClone ...
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bioRxiv - Cell Biology 2022Quote: ... we covalently labeled cell-surface proteins using a membrane-impermeable biotinylation reagent (NHS-SS-biotin; Thermo Fisher) as described previously (Yamada et al. ...
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bioRxiv - Pathology 2022Quote: ... followed by a rat anti-mouse IgG conjugated to biotin (Cat#13-4013-85, ThermoFisher, Basel, Switzerland) and a streptavidin conjugated Alexa546 (s11225 ...
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bioRxiv - Genomics 2019Quote: ... and the 5’ overhang was repaired using a biotinylated residue (0.4 mM biotin-14-Datp (INVITROGEN, America). The resulting blunt-end fragments were ligated in situ (10X NEB T4 DNA ligase buffer (NEB ...
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bioRxiv - Genetics 2019Quote: ... Cell surface proteins were labeled with cell membrane impermeable Sulfo-NHS-Biotin (#21217, Thermo Fisher, Waltham, MA), quenched with 100 mM glycine ...
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bioRxiv - Biophysics 2019Quote: ... a six-fold molar excess of photo-cleavable NHS-biotin cross-linker (Fisher Scientific, # NC1042-383, USA) was added to the Fabs (0.28 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... The respective ligands were biotinylated using the biotinylation kit EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). The biotin label was freshly resolved in H2O ...