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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... biotin labelled probes were detected using a Chemiluminenscent Nucleic Acid Detection Module (Thermo Scientific #89880) according to manufacturer’s instructions and imaged using a Kodak BioMaxXAR film.
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bioRxiv - Neuroscience 2024Quote: ... Adjacent sections were incubated with biotin-labelled mouse isotype (IgG1) control (MG115, Thermo Fisher Scientific), diluted to the same protein concentration as the primary 6E10 antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) and 1% penicillin/streptomycin (Gibco). BT-549 and HCC1806 were cultured in Roswell Park Memorial Institute medium (RPMI ...
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bioRxiv - Immunology 2021Quote: ... The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Molecular Biology 2021Quote: ... SDM79 and SDM80 were supplemented with 7.5 mg/l hemin and 10% FCS (Invitrogen), and cells were grown at 27°C continuously in these media ...
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bioRxiv - Immunology 2022Quote: ... and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Cancer Biology 2019Quote: ... and 10% FCS and 100 units/mL Penicilium and 100 μg/mL Streptomycin (Gibco). To generate overexpressing cells ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA constructs for Fc-NKG2D molecules were expressed in Expi293TM cells (Thermo Fisher Scientific) and dimeric secreted protein was purified by Protein A affinity chromatography (PierceTM #20334 ...
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bioRxiv - Physiology 2020Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were > 0.188 ng/ml and > 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Bioengineering 2021Quote: ... cells were blocked with an anti-Fc Receptor polyclonal antibody (Invitrogen 14-9161-73) for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... point FCS measurements with Alexa Fluor® 488 (Thermo Fisher Scientific, Waltham, MA, USA) dissolved in water at 20 nM were performed at the same laser power ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% of Penicillin-Streptomycin mixture (Gibco) at 37°C 5% CO2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... followed by incubation with HRP-conjugated goat anti-Fc antibody (Invitrogen, catalog number A18817). Stained cells were visualized using KPL TrueBlue peroxidase (SeraCare ...
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bioRxiv - Immunology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated antibody specific for the Fc region of human IgG (Invitrogen) was used as secondary antibody to detect antibody binding in ELISA experiments.
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bioRxiv - Microbiology 2021Quote: ... Absorbance at 450 nm was read using an accuSkan FC microplate reader (Fisher Scientific) with SkanIt software (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled anti-human IgG Fc antibody (Catalog# 31413, Thermo Fisher) was added to each well and incubated for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed once with RPMI medium (Gibco, w/o glucose, FCS and PSG) and incubated in the same medium for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% Fetal Calf Serum (FCS) and 1% Antibiotic Antimycotic (Gibco, Thermofisher, Netherlands) was added to each swab sample ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% Fetal Calf Serum (FCS) and 1% Antibiotic Antimycotic (Gibco, Thermofisher, Netherlands) was added to each swab sample ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Immunology 2022Quote: ... and pellet resuspended in 200uls MACS + Fc-block (ThermoFisher, catalog no. 14-9161-73). Cells were stained for surface antigens using fluorescence-conjugated monoclonal Abs specific to human CD45 ...
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bioRxiv - Immunology 2022Quote: ... Fcs and IgGs were synthesized and cloned by GeneArt (Thermo Fisher Scientific, Waltham, MA) in the pcDNA3.4 expression vector and transiently expressed in HEK 293F cells ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase (HRP)-conjugated Abs specific for the Fc region of human IgG (Invitrogen) were used as secondary antibodies to detect antibody binding in ELISA experiments ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... with glutamax supplemented with 10% FCS and 10 µg/ml Blasticidin (Invitrogen, R210-01). HCT 116 _CRISPR-Cas9 cells were cultured like the parental cell line supplemented with 2 µg/ml Puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... and 10% FCS in culture dishes (100 mm with vents; Fisher Scientific UK Ltd) at 37°C in a humidified incubator at 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... containing 10% FCS and 100 units/ml penicillin-streptomycin solution (Pen/Strep, Gibco, Belgium). Virus culture and cytopathic effect-based virus neutralization assays (CPENT ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and resuspended in FC buffer with goat-anti-mouse Alexa488 (Invitrogen; 1:400) for an additional 15 minutes ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowaste) and 1% penicillin/streptomycin (Life Technologies).
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bioRxiv - Synthetic Biology 2020Quote: ... Absorbance at 405 nm was recorded with Multiskan FC microplate photometer (Thermo Fisher Scientific). All shaking steps were conducted in Titramax 1000 (Heidolph Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... TβRII-Fc (124 kDa) and murine mAb 1D11 (150 kDa) (Thermo Fisher, Waltham, MA) were each captured on individual flow cells to 100-140 RU ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Physiology 2019Quote: ... The ELISA was read using a filter-based accuSkan FC micro photometer (Fisher Scientific). The limits of sensitivity were <0.188 ng/ml and < 0.375 ng/ml for MUC5AC and MUC5B ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Protease susceptibility measurements were performed in biological replicates (n = 2 ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82). Titrations were performed in biological replicates (cultures from independent colonies grown on separate days ...
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bioRxiv - Microbiology 2021Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was blocked by transferring the halves to a dish containing FCS (Gibco, USA) and the epidermis peeled off the dermis ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were blocked prior to staining using anti-CD16/32 (93, ThermoFisher Scientific). Where >1 brilliant violet or brilliant UV dye was used concurrently ...
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bioRxiv - Immunology 2020Quote: ... followed by addition to the Fc-chimeric human ACE2-coated MaxiSORP ELISA plate (Nunc) for an hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... followed by Fc-receptor blockade with anti-CD16/CD32 (Thermo Fisher #14-0161-85), and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... to remove clumps and 10 ml RPMI supplemented with 10% FCS (Thermo Fisher Scientific) was added ...
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bioRxiv - Microbiology 2023Quote: Cells were then frozen in fetal calf serum (FCS) (ThermoFisher Scientific, Cat. No. 10500064) 10% DMSO (v/v ...
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bioRxiv - Immunology 2022Quote: ... Optical density (OD) was measured using a Multiskan™ FC (Thermo Fisher-Scientific UK) plate reader at 405 nm and S1-specific IgG titers were interpolated from the IgG standard curve using 4PL regression curve-fitting on GraphPad Prism 9.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% v/v foetal calf serum (FCS) (Thermo Fisher Scientific, Waltham, MA) and 1% v/v penicillin/ streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... Medium was removed and cells were transferred in FCS-free essential medium OptiMEM (Gibco) 2 h before transfection ...
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bioRxiv - Microbiology 2024Quote: ... 10% (v/v) heat inactivated fetal calf serum (FCS, Hyclone, Thermo Scientific, Rockford, IL), 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human Fc chip was regenerated to baseline using IgG Elution Buffer (Thermo Scientific) and the process was repeated for all humAbs in the panel ...