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Citations for Thermo Fisher :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... penicillin/streptomycin (100 mg/ml) (Gibco) in EBSS (calcium ...
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bioRxiv - Synthetic Biology 2024Quote: ... The reconstituted library was then transformed into MegaX DH10B T1 R electrocompetent cells (ThermoFisher), cultured and purified for the initial RISE cycle ...
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bioRxiv - Synthetic Biology 2024Quote: ... RT-PCR of this rRNA was done using SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) in 100 μL reactions according to product literature ...
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bioRxiv - Synthetic Biology 2024Quote: ... visualized with SYBR gold stain (Thermo Fisher), and quantified using Image J software63.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 10^6 cells were blocked with purified anti-mouse CD16/CD32 (1:100, clone 93, Cat. No. 14-0161-82, eBioscience, Thermo Fisher Scientific) before incubating for 30 min on ice with the following cocktail ...
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bioRxiv - Neuroscience 2024Quote: ... using Power SYBR Green Master Mix (Thermo Fisher), 300 nM of primer pair and 10 ng of cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... The zebrafish were then embedded in Shandon Cryomatrix embedding resin (Thermo Scientific, 6769006), and frozen sections were cut at 25 µm using a Leica CM3050S cryostat ...
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bioRxiv - Neuroscience 2024Quote: ... penicillin/streptomycin (100 mg/ml) (Gibco). Control SH-SY5Y cells were cultured in DMEM/F12 with FBS (10% ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was amplified using a polymerase (DreamTaq PCR Master Mix 2X, Thermo Fisher Scientific K1071), 60°annealing temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were mounted dorsal side-up (axial view) or lateral side-up (sagittal view) in 2% low-melting point agarose (Thermo Fisher Scientific 16520) in E3 ...
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bioRxiv - Neuroscience 2024Quote: ... containing phosphatase and protease inhibitors (Thermo Fisher Scientific). Homogenates were centrifuged at 13000× g for 15 min 4 ° ...
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bioRxiv - Neuroscience 2024Quote: ... in EBSS (calcium, magnesium, phenol red) (Gibco) for 6 h was used for high proteolysis/pro autophagic (starvation ...
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bioRxiv - Neuroscience 2024Quote: RNA isolation was performed using an RNAqueous Micro Total RNA Isolation Kit (Thermo Fisher Scientific). RNA concentration and quality (RIN > 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were sorted into an Eppendorf tube containing 700 µl of lysis buffer (RNAqueous Micro Total RNA Isolation Kit, Thermo Fisher Scientific) for downstream bulk RNA sequencing.
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bioRxiv - Neuroscience 2024Quote: ... and phenol red (EBSS, Thermo Fisher Scientific 24010043) and mounted dorsal-up in 2% agarose ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were transfected with Lipofectamine 3000 with pcDNA3.1 (-) expression vector (Invitrogen, USA) encoding either FPR1 or FPR2 fused to a FLAG-tag.
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bioRxiv - Neuroscience 2024Quote: ... Tol2 mRNA was obtained by in vitro transcription using the mMESSAGE mMACHINE SP6 transcription kit (Ambion, AM1340). The transgenic vector containing the sensor C3 gene (final concentration ...
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bioRxiv - Plant Biology 2024Quote: ... secondary Alexa Fluor 488 antibodies (diluted 1:200 in 0.1% BSA in PBS, goat Anti-Rat-IgM, Thermo Fisher Scientific, Denmark) were added and the sections were incubated overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were added to each well on a 96-well plate (Nunc MaxiSorpTM flat-bottom, Thermo Fisher Scientific, Denmark) and immobilized at 37°C for 72 h with shaking (350 rpm) ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were added to each well on a 96-well plate (Nunc MaxiSorpTM flat-bottom ...
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bioRxiv - Plant Biology 2024Quote: ... sodium metabisulphite (Thermo Scientific, USA) was gradually added to the precipitate and heated at 50°C to reduce the diazo linkage until the suspension was decolourised ...
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bioRxiv - Plant Biology 2024Quote: ... Dynabeads was magnetically precipitated using a DynaMagnetic rack (Thermo Fisher) and then washed five times with 0.5 mL of ice-cold 1XPBS buffer to eliminate non-specific binding ...
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bioRxiv - Plant Biology 2024Quote: ... Purified total RNAs were quantified by Qubit (Invitrogen) fluorimeter ...
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bioRxiv - Plant Biology 2024Quote: ... Purified total RNAs were quantified by Qubit (Invitrogen), RNA quality was tested using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... The EIN2-C native antibody was prebound with the equilibrated protein-G coated magnetic beads (Dynabeads, Thermo Fisher) with 1% BSA for 3 h with gentle rotation at 4 °C and incubated at 4 °C after cleared cell fractionation addition with gentle rocking overnight for Co-IP ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were then treated with DNase I (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Reactions were done using the 7500 Fast system (Applied Biosystems, ABI), and the data were analyzed using ABI Prism 7700 SDS software (ABI) ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Quantification of transcript was done by qRT-PCR with fluorescent-labelled nucleotide substrate (TB Green™ Premix Ex Taq™ II, Takara or PowerUp SYBR Green Master Mix, Thermo Scientific) as described previously (Shigeyama et al ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from 20 mg seeds (dry weight) using the RNAqueous™ Small scale phenol-free total RNA isolation kit (Ambion: catalog #1912) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Plant Biology 2024Quote: The purity of the extracted RNA was assessed based on the OD260 nm/OD280 nm ratio determined by a NanoDrop instrument (Thermo Fisher Scientific, USA) and agarose gel electrophoresis was performed to test for RNA degradation and potential contamination ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The cycle threshold (Ct ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was treated with DNAse 1 (ThermoFisher), and cDNA was synthesised with M-MLV reverse transcriptase (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using TRI-reagent (Invitrogen), RNA was treated with DNAse 1 (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: ... The NAC5-A1 sequence was then recombined into the Gateway-compatible binary vector pSC4-Act-R1R2 in an LR Clonase reaction (ThermoFisher) to create pMSH30 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA (typically 1 μg) was reverse transcribed using SuperScript III (Invitrogen). Real-time quantitative PCR assays were prepared using KAPA SYBR FAST qPCR kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... the peptides were sampled on a precolumn (300 μm x 5 mm PepMap C18, ThermoFisher, US) and separated in a 75 μm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, ThermoFisher, US) using a 180-min gradient ...
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bioRxiv - Plant Biology 2024Quote: ... The MS and MS/MS data were acquired using Xcalibur (V2.8, ThermoFisher, US).
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA in the samples was removed using TURBO™ DNase (Thermo Fisher Scientific) followed by purification with the Monarch RNA Clean Up Kit (NEB) ...
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bioRxiv - Plant Biology 2024Quote: The 5,689 bp ABCC10 genomic sequence was amplified from Arabidopsis (Col-0) genomic DNA using Pfx platinum (Life Technologies) polymerase in three successive overlapping fragments using primers ABCC10-caccPmlI (5’-CACCACGTGATGATAGAAAATTATTGGACTTCC -3’ ...
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bioRxiv - Plant Biology 2024Quote: ... while the other two were cloned into the pCR4 vector (Life Technologies). Overlap extension PCR cloning (Bryksin and Matsumura ...
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bioRxiv - Plant Biology 2024Quote: ... The first of the ∼2 kb fragments was cloned into the pENTR/D-TOPO vector (Invitrogen), while the other two were cloned into the pCR4 vector (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... 1 ml of TRIZOL (Invitrogen, Carlsbad, CA, USA) and 200 µl of chloroform were used for RNA isolation according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were grown horizontally for 4dy under continuous white light before staining in aqueous propidium iodide (10 μg/mL; Molecular Probes) to label cell walls ...
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bioRxiv - Plant Biology 2024Quote: ... This ABCC10 gDNA was recombined into the pEarlyGate104 plant expression vector (Earley et al., 2006) using LR Clonase (Invitrogen) to form pEarleyGate104-ABCC10g which expresses N-terminal YFP with ABCC10 driven by the cauliflower mosaic virus 35S promoter ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were grown horizontally for 4dy under continuous white light before counterstaining in amphiphilic styryl dye FM4-64 (2 μM; ThermoFisher) on ice to label the plasma membrane (Rigal et al. ...
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bioRxiv - Plant Biology 2024Quote: ... and capped run-off transcripts were generated using T7 RNA polymerase (MEGAscript T7 transcripton kit, Thermo Fisher). mRNAs were translated in wheatgerm extract (Promega ...