Labshake search
Citations for Thermo Fisher :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... Sectioning was done at 3 um thickness using the automatic microtome (HM355S, Thermo Fisher Scientific). Sections were mounted onto Superfrost Plus Adhesion Microscope Slides (J1800AMNZ ...
-
bioRxiv - Genetics 2024Quote: ... The supernatant chromatin was pre-cleared with 10 μl of 1:1 protein A: protein G Dynabeads (Thermo Fisher Scientific, 10001D and 10003D). The precleared chromatin sample was incubated with 0.5 μg of anti-H3K4me3 (Millipore 17-614 ...
-
bioRxiv - Genetics 2024Quote: ... All injection mixes contained 300 ng/µL Cas9 nuclease (Invitrogen, California, USA) and 300 ng/µL purified sgRNA ...
-
bioRxiv - Immunology 2024Quote: ... Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes, Life Technologies) and qualification was done using the Agilent TS D1000 kit and TapeStation 4200 ...
-
bioRxiv - Biochemistry 2024Quote: ... operated at 200 kV using an Eagle 4k_4k camera (ThermoFisher Scientific). Measurements were manually done with Image J (https://imagej.net/ij/ ...
-
bioRxiv - Biochemistry 2024Quote: ... Human calpain-3 isoform_1 (NM_000070.3) was used as a template from which GeneArt (Thermo Fisher Scientific) synthesized the modified gene following codon optimization ...
-
bioRxiv - Genetics 2024Quote: Total RNA was isolated from the whole body and integument of the silkworms using TRIzol reagent (Invitrogen, California, USA), purified by phenol chloroform extraction and then reverse transcribed with a PrimeScript™ RT Reagent Kit (TAKARA ...
-
bioRxiv - Genetics 2024Quote: ... Sections were mounted onto Superfrost Plus Adhesion Microscope Slides (J1800AMNZ, Thermo Fisher Scientific) and dried at 37°C overnight ...
-
bioRxiv - Genetics 2024Quote: ... Melting temperatures were calculated using the Protein Thermal Shift software (Applied Biosystems).
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized with the SuperScript™ III First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... The lysates were separated by NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories ...
-
bioRxiv - Genetics 2024Quote: ... 1% SDS (Fisher Scientific cat no. BP166-100)) ...
-
bioRxiv - Genetics 2024Quote: ... The assay was conducted at 10 μL volumes with 10X SYPRO Orange dye (Invitrogen) and nucleosome dilution buffer (10 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Biochemistry 2024Quote: ... RNAs were passed through NucAway Spin columns (Invitrogen) for purification ...
-
bioRxiv - Biochemistry 2024Quote: ... U2OS-265 (34) and U2OS DR-GFP (ATCC CRL-3455) cells were grown in DMEM (ThermoFisher Scientific 10569044) supplemented with 10% FBS (R&D solutions S11150 ...
-
bioRxiv - Biochemistry 2024Quote: ... Concentration of the dialyzed fractions were then quantified using Qubit 4 fluorometer (Invitrogen, REF Q33226), flash frozen ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell line was maintained in culture at a density of 3×105 cells/ml in RPMI medium (Gibco-Life Technologies) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein concentration was confirmed with either a UV-vis (Thermo Scientific, GENESYS 50 UV-visible spectrophotometer ...
-
bioRxiv - Biochemistry 2024Quote: ... Protein samples were then quantified with the Qubit Protein Assay from ThermoFisher Scientific (catalog# Q33212) ...
-
bioRxiv - Biochemistry 2024Quote: ... Fluorescent probes dihydroethidine and 2’,7’-dichlorodihydrofluorescein diacetate were obtained from Molecular Probes (Eugene, Oregon, USA).
-
bioRxiv - Biochemistry 2024Quote: ... or a Qubit (Life Technologies, Qubit 3.0 Fluorometer). 20 μL aliquots of the samples were deposited on a 0.05 mm quartz cuvette and measured in a Circular Dichroism (CD ...
-
bioRxiv - Biochemistry 2024Quote: ... LDH activity was measured as the kinetics of the decrease in NADH absorption at 340 nm for a minute in NanodropOne (Thermo Scientific). Percent protection was calculated as a ratio of NADH absorbance for the desiccated samples normalized to non-desiccated controls ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell line was maintained in culture at a density of 3×105 cells/ml in RPMI medium (Gibco-Life Technologies) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biophysics 2024Quote: ... 2 µl Lipofectamine LTX (Invitrogen, Carlsbad, USA) and 500 ng of the plasmid DNA was prepared and added to a well with 400 µl of DMEM+ ...
-
bioRxiv - Biophysics 2024Quote: ... The cryo-EM data were collected using either 300[keV Krios microscope (Thermo Fisher), equipped with Gatan K3 detector (all dataset except for CryoR1 at pH 8.0) ...
-
bioRxiv - Biophysics 2024Quote: ... 0.2 M NaCl and 1% DDM (Anatrace, Affymetrix) and stirred overnight for solubilization ...
-
bioRxiv - Biophysics 2024Quote: ... or 200 keV Talos Arctica (Thermo Fisher), equipped with Gatan K2 Summit detector (CryoR1 at pH 8.0).
-
bioRxiv - Genetics 2024Quote: ... in a total volume of 2000 μL with nuclease free water (Invitrogen cat no. AM9922) and incubated at 56°C for 18 h ...
-
bioRxiv - Genetics 2024Quote: Nucleosome stability was measured using a Protein Thermal Shift kit (Applied Biosystems) with the following modifications ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using SuperScript™ VILO cDNA Synthesis kit (ThermoFisher Scientific). RT-PCR to detect nox2 missplicing used primers ...
-
bioRxiv - Genetics 2024Quote: ... cells were reverse crosslinked by treatment with 100 μL (20 mg/mL) Proteinase K (Thermo Fisher Scientific cat no. FEREo0491), 100 μL 10% SDS (Fisher Scientific cat no ...
-
bioRxiv - Microbiology 2024Quote: ... coupled online to a Q Exactive™ HF mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). 5 µL sample was injected onto a PepSep column (15 cm length ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA and RNA concentrations were measured using the Qubit 3.0 Fluorometer according to manufacturer’s protocol (Life Technologies).
-
bioRxiv - Cancer Biology 2024Quote: ... Tissue slides were then washed in UltraPure water (Invitrogen 10977-023) for 5 min at RT ...
-
bioRxiv - Cancer Biology 2024Quote: ... and subsequently incubated in lysis buffer and washed with wash and resuspension buffer (WRB) (1x PBS with 1% BSA, 2U/ml RNAse inhibitor (Invitrogen, #10777019) and Hoechst (Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... and aspirates were dried at RT overnight and resuspended in 10 µL of UltraPure water (Invitrogen 10977-023). Each aspirate was then uniquely indexed using the Illumina i5×i7 dual indexing system through Nanostring NGS library preparation kits (Nanostring ...
-
bioRxiv - Cancer Biology 2024Quote: ... Psen1-/- Psen2-/- mouse embryonic fibroblasts (MEFs) and HEK293T/17 cells were cultured in DMEM/F-12 (Fisher scientific) with 10% FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Transwell insert plates were taken from ThermoFisher Scientific and matrigel from Corning ...
-
bioRxiv - Cancer Biology 2024Quote: ... HPB-ALL) with the combination of a sgRNA and HDR oligo using the MPK5000 nucleofector from Thermofisher. Next ...
-
bioRxiv - Microbiology 2024Quote: ... all reagents were purchased from ThermoFisher Scientific.
-
bioRxiv - Cancer Biology 2024Quote: ... they were digested by Proteinase K (0.5 µg/mL) (Thermo Fisher Scientific, AM2546) for 5 min at 37 °C ...
-
bioRxiv - Immunology 2024Quote: ... and 10% heat-inactivated human AB-positive serum (Invitrogen) at 37°C in 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed with PowerSYBR (Applied Biosystems) using a StepOnePlus Real-Time-PCR system (Applied Biosystems) ...
-
bioRxiv - Biophysics 2024Quote: ... Samples were loaded on a DNAPac PA200 column (22×250 mm; Thermo Fisher, Thermo Fisher Ultimate 3000 system) at 75 °C under low salt conditions in buffer A (20 mm NaOAc pH 6.5 ...
-
bioRxiv - Biophysics 2024Quote: ... Absorbance measurements were performed at 595 nm using a Varioskan LUX multimode microplate reader (Thermo Fisher). The loading efficiency was estimated using a northern blot protocol optimised for detection of small RNAs35 ...
-
bioRxiv - Biophysics 2024Quote: Human myeloma cells MM1.S (ATCC: CRL-2974) were cultured in RPMI 1640 cell media (Gibco) supplemented with 10% FBS and 1% penicillin-streptomycin ...
-
bioRxiv - Biophysics 2024Quote: ... and methyl-(PEG)4-NHS (Life technologies) in 1 x PBS were introduced and incubated for 1 h [120] ...
-
bioRxiv - Biophysics 2024Quote: ... SuperSignal® West Femto (ThermoFisher Scientific, USA) was used for protein band detection ...
-
bioRxiv - Biophysics 2024Quote: 10 mL AGO2 baculovirus stock was used to transfect each litre of Sf9 cells (1.5×106 cells/mL, passage 22, grown in Sf-900 II SFM media; Thermo Fisher). In total ...
-
bioRxiv - Biophysics 2024Quote: ... using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Expression vector pfastBac1 (Thermo fisher ...