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Citations for Thermo Fisher :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 1:1000 insulin-selenium-transferrin (Gibco), 16.7 µM zinc sulfate ...
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bioRxiv - Physiology 2024Quote: ... The guts were mounted in 50% glycerol and visualized with the EVOS FL Auto live-cell imaging system (Life Technologies, Burlington, ON).
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bioRxiv - Neuroscience 2024Quote: ... and transiently transfected with Lipofectamine 2000 (Thermo Fisher Scientific). When necessary ...
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bioRxiv - Neuroscience 2024Quote: ... along with phalloidin 405 (1:1000; Thermo Fisher Scientific). Samples underwent two 15-minute washes in PBT with rotation followed by a brief 5-minute wash in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Kv2.1-CHO cells were detached in a PBS-EDTA solution (Gibco Cat# 15040-066), spun at 500 g for 2 minutes and then resuspended in 50% cell culture media and 50% CHO-external recording solution ...
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bioRxiv - Neuroscience 2024Quote: ... and then magnetic protein G beads (ThermoFisher Cat# 10004D) were added for an additional 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated with mouse IgG subclass- and/or species-specific Alexa-conjugated fluorescent secondary antibodies (Invitrogen) diluted in blotto-T for 45 min and washed 3 x 5 minutes in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was incubated overnight at 4 °C with the anti-Kv2.1 rabbit polyclonal antibody KC (Trimmer, 1991) and then incubated with 100 μL of magnetic protein G beads (ThermoFisher Cat# 10004D) for 1 h at 4 °C on a tube rotator ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then washed extensively in water and mounted with Prolong Gold (ThermoFisher Cat # P36930).
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated for 20 minutes at 37 °C with 1µg/mL α-bungarotoxin-AlexaFluor647 (Invitrogen #B35450) dissolved in HEK293T cell culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... 1% penicillin/streptomycin (Invitrogen) and 10 mM Glutamate.
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bioRxiv - Neuroscience 2024Quote: The pellet was washed with 1mL of 75% Ethanol and resuspended in DEPC-treated RNase free water (Thermo Fisher Scientific). 0.5 to 1.5µg of RNA was used to generate cDNA using SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit-hosted antibodies [anti-Claudin-5 (1:100, 34-1600, Invitrogen), and anti-Iba-1 (1:1000 ...
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bioRxiv - Physiology 2024Quote: ... 1% penicillin/streptomycin (Life Technologies, cat. 15140122), and 1% Glutamax (Life Technologies ...
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bioRxiv - Physiology 2024Quote: ... and HRP-conjugated secondary antibodies (Thermo Fisher, 1:10,000). Specific protein bands were visualized and evaluated using FluorChem HD2 (Protein Simple).
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bioRxiv - Physiology 2024Quote: ... and 1% Glutamax (Life Technologies, cat. 35050061) at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Physiology 2024Quote: ... in MOPS running buffer (Invitrogen,) and then transferred to nitrocellulose membranes (GE Healthcare Life science) ...
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bioRxiv - Physiology 2024Quote: ... water bath for 3 minutes at 40% power and then mixed with a 1 to 20 solution of Lipofectamine 2000 (Thermo Fisher Scientific, cat. 11668019) and Opti-MEM ...
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bioRxiv - Physiology 2024Quote: ... An equal amount of protein (20 µg) was separate on 4%-12% bis-tris polyacrylamide gel (Invitrogen) in MOPS running buffer (Invitrogen, ...
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bioRxiv - Physiology 2024Quote: ... Human neutrophils were cultured in RPMI-1640 media (Gibco, #52400017) + 10% FCS + 1% penicillin/streptomycin and the purity of isolated neutrophils was confirmed as >98% CD45+ CD15+ via flow cytometry (anti-CD45-FITC ...
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bioRxiv - Physiology 2024Quote: ... 1× N2 Supplement (Gibco™, #17502048), 1× B27™ Supplement (Gibco™ ...
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bioRxiv - Physiology 2024Quote: ... 1× B27™ Supplement (Gibco™, #17504044), 1 mM N-acetylcysteine (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... 10 mM HEPES (Gibco™,#15630080), 1× N2 Supplement (Gibco™ ...
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bioRxiv - Physiology 2024Quote: ... The flask was then returned to the hood and 100 ml of filtered (Grade F) 30% H2O2 (>95%, Thermo Scientific, Nepean) was added ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (GFAP; 1:1500, MA5-12023, Invitrogen), anti-CD68 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... 120 mL ethylene glycol (Thermo Fisher Scientific), 200 mL 0.1 M PB] at −20°C until processing.
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bioRxiv - Neuroscience 2024Quote: ... protein signals were detected by the ChemiDoc MP imaging system using the SuperSignal West Pico PLUS Chemiluminescent Substrate or the SuperSignal West Femto PLUS Chemiluminescent Substrate (Thermo Fisher Scientific). Fiji software (ImageJ ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-Iba-1 (1:1000, 019-19741, Wako) were visualized using goat-anti-rabbit Alexa Fluor 555 (1:500, A21428, Invitrogen). Wisteria floribunda lectin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: Mouse-hosted antibodies [anti-rat endothelial cell antigen (RECA-1; 1:50, MA1-81510, Invitrogen), anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2024Quote: ... Wisteria floribunda lectin (1:1000, B-1355-2, Vector) was visualized using Streptavidin 568 (1:500, S11226, Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... The cortex was isolated and digested with 0.1% type IV collagenase with 30 u/ml DNase I (Gibco) at 37 °C for 1.5 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% Penicillin-Streptomycin (Gibco). Half of the culture medium was replaced every three days ...
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bioRxiv - Neuroscience 2024Quote: ... digested with 0.25% trypsin (Gibco) at 37 °C for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and terminated the digestion with a complete medium with DMEM-F12 (Gibco), 10% FBS ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from cultured muscle cells using TRIzol-cholorform extraction according to manufacturer’s instruction (Invitrogen). RNA concentration and purity were determined by spectrophotometry ...
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bioRxiv - Physiology 2024Quote: HEK293T cells were grown in DMEM media (Gibco #10569) supplemented with 10% foetal bovine serum ...
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bioRxiv - Neuroscience 2024Quote: ... The final libraries were quantified using the Qubit High Sensitivity DNA assay (Thermo Fisher Scientific, Cleveland, OH, USA). The size distribution of the libraries was determined using a High Sensitivity DNA chip on a Bioanalyzer 2200 (Agilent ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Supernatants were immunoprecipitated either with Arc antibody (rabbit polyclonal, custom-made; ThermoFisher) or normal rabbit IgG (Santa Cruz Biotechnology ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... generated by Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were resuspended with a complete medium containing DMEM-F12 (Gibco), 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... and terminated the digestion with high sugar Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) complete medium (Gibco). The cell suspension was collected by a 100 µM nylon cell filter and centrifuged at 800 rpm for 5 minutes ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... or 647 for the appropriate animal host (1:1000 Thermofisher Scientific).
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... a 10% volume of washed Protein A/G magnetic bead slurry (ThermoFisher Scientific) was added to the antibody/lysate mixture and incubated for 1 hour at 4°C rotating ...
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bioRxiv - Neuroscience 2024Quote: ... The final libraries were quantified using the Qubit High Sensitivity DNA assay (Thermo Fisher Scientific, Cleveland, OH, USA). Their size distribution was determined using a High-sensitivity DNA chip on a Bioanalyzer 2200 (Agilent ...
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bioRxiv - Physiology 2024Quote: ... The concentration of total RNA was measured using the NanoDrop2000 (Thermo Fisher Scientific, USA), and RNA purity was assessed by 260/280 and 260/230 ratios ...
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bioRxiv - Physiology 2024Quote: ... and AlexaFluor 647 conjugated WGA (5 µg/ml) (cat no: W32466, Thermo Fisher Scientific, Waltham, MA) for 30 min followed ...
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bioRxiv - Physiology 2024Quote: ... Oligonucleotides with Bsb1 cleavage overhang were ordered from Life Technologies, annealed ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with DNase- and RNase-free water (catalog no. 10977023, Invitrogen™, MA, USA). Stained sections were imaged under a microscope (ECLIPSE Ti ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed using the high-capacity RNA-to-cDNA™ kit (Applied Biosystems, Massachusetts, USA).
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... Neurons on coverslips were mounted on glass slides in Fluoromount (ThermoFisher Scientific) and dried overnight at RT.