Labshake search
Citations for Thermo Fisher :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: ... 1.5 μL poly- dT Dynabeads (Thermo Fisher)) per reaction ...
-
bioRxiv - Biophysics 2024Quote: ... 1 M potassium chloride and 5 M sodium chloride were obtained from our in-house media kitchen. Pure water was obtained from a MilliQ water filtration system. SYBR Safe (catalog no. S33102) was obtained from Invitrogen. Neutravidin protein (catalog no ...
-
bioRxiv - Biophysics 2024Quote: ... The cDNA was eluted from Dynabeads using 11 μL Formamide-ROX mix (1000 μl Hi-Di Formamide (Thermo Fisher), 8 μl of 350 ROX size standard (Thermo Fisher) ...
-
bioRxiv - Biophysics 2024Quote: ... Expression vector pfastBac1 (Thermo fisher) was used to generate tβ1AR and mutant isoform (L72A ...
-
bioRxiv - Biophysics 2024Quote: ... 40 nm dark-red beads (catalog no. F8789) and 40 nm red-orange beads (catalog no. F8794) were obtained from Invitrogen. Norland Optical Adhesive 74 (catalog no ...
-
bioRxiv - Biophysics 2024Quote: ... and 1% Glu-taMAX (Life Technologies) at 37 °C under 10% CO2.
-
bioRxiv - Biophysics 2024Quote: ... HEK293T cells (purchased from ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Biophysics 2024Quote: ... Neutravidin protein (catalog no. 31000) was obtained from Thermo Scientific. Polyethylene glycol (PEG)-8000 (catalog no ...
-
bioRxiv - Biophysics 2024Quote: ... in Opti-MEM (Gibco) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... 8 μl of 350 ROX size standard (Thermo Fisher)) per reaction and incubating at room temperature for 15 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... mtDNA were amplified using specific primers with the Fast SYBR™ Green Master Mix (ThermoFisher, 4385610). Primers sequence for RT-qPCR were as follows ...
-
bioRxiv - Biophysics 2024Quote: ... Streptavidin-Cy5 (10 µM, ThermoFisher Scientific) were introduced into the respective sample groups ...
-
bioRxiv - Biophysics 2024Quote: ... Ligated vectors were transformed into competent bacteria (One Shot TOP10 E. coli; Invitrogen) and grown overnight on ampicillin plates ...
-
bioRxiv - Biophysics 2024Quote: ... The constructs (tβ1AR and L72A mutant isoform) were over expressed in insect cells (sf9, Invitrogen, 11496015) using the Bac-to-Bac baculovirus expression system (Thermo Fisher) ...
-
bioRxiv - Biophysics 2024Quote: ... 0.1 μL SuperScript III Reverse Transcriptase (Thermo Fisher)) were added ...
-
bioRxiv - Neuroscience 2024Quote: ... washed with 0.1M PBS and incubated for 3 hours in a 25:1 solution of 0.1M PBS with 435/455 blue or 530/615 red fluorescent NeuroTrace Nissl stain (Invitrogen). Brain sections were imaged and stitched using a VS200 Virtual Slide fluorescence microscope (Olympus ...
-
bioRxiv - Immunology 2024Quote: ... Red blood cells were lysed with ACK Lysis buffer (Gibco) and cells resuspended in RPMI with 10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... using a StepOnePlus Real-Time-PCR system (Applied Biosystems). Expression of each gene was normalized to β2M ...
-
bioRxiv - Microbiology 2024Quote: ... were mixed with 4X Sample Buffer (“4X SB”: 250 mM Tris pH 6.8, 2% Dodecyl sulfate sodium salt (Acros Organics), 20% β-mercaptoethanol ...
-
bioRxiv - Immunology 2024Quote: ... and were used fresh or after freezing in FCS (Invitrogen) containing 10% DMSO (Sigma-Aldrich).
-
bioRxiv - Immunology 2024Quote: Real-time PCR was performed with PowerSYBR (Applied Biosystems) using a StepOnePlus Real-Time-PCR system (Applied Biosystems) ...
-
bioRxiv - Immunology 2024Quote: ... Slides were mounted in Prolong Diamond (ThermoFisher) and curated during 24 hours at room temperature before imaging ...
-
bioRxiv - Immunology 2024Quote: ... 1.8 µm) the outlet of which was connected to an LTQ-Orbitrap XL MS System (ThermoFisher Scientific, USA and Waters, USA). The LC flow rate was set to 150µL/min and the column oven temperature was adjusted to 35°C ...
-
bioRxiv - Immunology 2024Quote: ... and cDNA was synthesized using Superscript-III First-Strand Synthesis System (Life Technologies). Real-time PCR was performed with PowerSYBR (Applied Biosystems ...
-
bioRxiv - Immunology 2024Quote: ... using a StepOnePlus Real-Time-PCR system (Applied Biosystems). The expression of each gene was normalized to a negative region ...
-
bioRxiv - Immunology 2024Quote: ... 100 mg/ml streptomycin (all obtained from Life Technologies), and 10% heat-inactivated human AB-positive serum (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... Ten L3 per well were cultured in triplicate at 37°C and 5% CO2 for 1 week in 96-well plates for cell culture (Thermo Fisher Scientific) with 200 µL of medium containing 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2024Quote: HEK TER cell lines were suspended in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and mixed with 0.4% Noble agar ...
-
bioRxiv - Microbiology 2024Quote: ... The blot was incubated with a chemiluminescence kit (Roche 12015200001, Thermo Scientific™ 34096) and images were captured using ChemiDoc™ Imaging System (Biorad 12003153) ...
-
bioRxiv - Microbiology 2024Quote: ... cells were washed twice with sterile PBS and incubated with 1 µg/ml Bodipy 493/503 (D3922, Invitrogen) for 10 minutes at 30°C and 200 rpm ...
-
bioRxiv - Microbiology 2024Quote: ... data collection was carried out using Tomo software (Thermo Fisher Scientific), at a nominal magnification of x31,000 with a calibrated pixel size of 2,78Å ...
-
bioRxiv - Neuroscience 2024Quote: ... Muscles were then incubated for 15 minutes with rhodamine-conjugated α-bungarotoxin (Invitrogen, Carlsbad, CA), permeabilized in −20°C methanol for 5 min ...
-
bioRxiv - Neuroscience 2024Quote: ... EdU incorporation was identified using the Click-iT EdU Alexa Fluor 555 labeling kit (Molecular Probes); the recommended protocol was followed ...
-
bioRxiv - Microbiology 2024Quote: Cryo-electron tomography: Tilt-series were acquired on a Talos Arctica (Thermo Fisher Scientific) operated at 200 kV in parallel beam condition using either a K2 (for mutant cells ...
-
bioRxiv - Microbiology 2024Quote: HEK TER cells were described previously and cultured in Minimum Essential Media (MEM, Gibco) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Microbiology 2024Quote: Samples (equivalent to 0.6 µl culture at OD600nm = 1) were collected after reaching stationary phase and deposited onto GeneFrames (ThermoFisher) containing solidified C-/N-free M9 medium with 1 % agarose ...
-
bioRxiv - Genomics 2024Quote: ... RNA was then treated with TURBO DNA-free™ Kit (Invitrogen™) to ensure removal of remnant DNA ...
-
bioRxiv - Microbiology 2024Quote: ... Next generation DNA sequencing on an Ion S5 System (Thermo Fisher Scientific, France) was performed at the GeT_Biopuces platform (TBI ...
-
bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the pooled MT samples using PureLink™ RNA Mini Kit (Thermo Fisher Scientific) and stored at −80°C ...
-
bioRxiv - Microbiology 2024Quote: ... The coverslips were mounted onto glass slides using ProLong Gold Antifade Mountant (Invitrogen, P36934) and analyzed by confocal microscopy (Olympus FV3000RS ...
-
bioRxiv - Microbiology 2024Quote: ... The qPCR runs were performed using Applied Biosystems™ StepOne™ Real-Time PCR System (Thermo Fisher Scientific) and THUNDERBIRD® SYBR qPCR Mix (Toyobo) ...
-
bioRxiv - Microbiology 2024Quote: ... the pellet was evaporated to dryness with an Orbitrap apparatus (Thermo Fisher Scientific, USA) and hydrolyzed for 16h at 105°C with 500 µL HCl 6N ...
-
bioRxiv - Microbiology 2024Quote: ... using an UltiMate 3000 system (NCS-3500RS Nano/Cap System; Thermo Fisher Scientific) coupled to an Orbitrap QExactive Plus mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... quantified with a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, France) and assembled by In-Fusion (TaKaRa ...
-
bioRxiv - Genomics 2024Quote: mRNA was isolated using TRIzol™ LS Reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... cDNA was prepared using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific™). qPCRs were done using GoTaq qPCR Master mix (Promega ...
-
bioRxiv - Cell Biology 2024Quote: ... The plunge-freezing was performed using a Mark IV Vitrobot (Thermo Fisher). Frozen samples were then transferred into a pre-cooled 2-ml screw cap tube (Cat# HS10060 ...
-
bioRxiv - Developmental Biology 2024Quote: ... using iBlot2 system (Invitrogen). After transfer membranes were blocked in a blocking buffer (5% non-fat dry milk in TBS-T ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were seeded with 10 μl of 1×106 cells/ml in a Falcon 24-well cell culture plate (Cat# 353047, Fisher Scientific) and the plate was incubated in the cell-culture incubator (37 °C and 8% CO2 ...
-
bioRxiv - Cell Biology 2024Quote: ... were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented by 10% (v/v) fetal bovine serum and 100 units/ml of penicillin-streptomycin (Cat# 15140122, Thermo Fisher). Cell subculture was performed twice a week to maintain cell viability and proliferation ...