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Citations for Thermo Fisher :
551 - 600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates of cells were sonicated with a Sonic Dismembrator Model F60 (remote, 10 seconds at 50% power, then 2 minutes on ice, repeated three times; Fisher Scientific) and passed through a Hamilton syringe (14-824-663 ...
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bioRxiv - Cancer Biology 2024Quote: ... and addition of Laemmli sample buffer [0.5M Tris HCl at pH 6.8 (50-213-711; Fisher Scientific), 10% sodium dodecyl sulfate (SDS) ...
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bioRxiv - Cancer Biology 2024Quote: PDX4 cells were plated at 25% confluence in three 25 cm2 tissue culture flasks (12-556-009; Thermo Fisher Scientific, Waltham, WA, USA). After 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using SYBR Green Master Mix on a StepOnePlus Real-time PCR system (Thermo Fisher Scientific). Gene expression was normalized to 18s rRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... We simultaneously determined the concentrations of cytokines in supernatant samples using a customized 28-plex immunoassay kit (ProcartaPlex Mouse 28-plex, ThermoFisher Scientific), which employs magnetic beads for detection ...
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bioRxiv - Cancer Biology 2024Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Nunc) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured in DMEM (Gibco, 11965) supplemented with 10% FBS (Gemini Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... Bones were washed with BMDM media (20% FBS, 1% sodium pyruvate, 0.1% β-mercaptoethanol, 10% conditioned supernatant from 3T3 fibroblasts, in Gibco DMEM containing glucose and 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: Mouse prostate tissue was treated with RNAlater Stabilization Solution (AM7020, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone homogenate was passed through a 70 μm nylon Corning Falcon cell strainer (Thermo Fisher Scientific, 08-771-2) to remove particulates ...
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bioRxiv - Cancer Biology 2024Quote: ... and with GeneMapper® v5 (Thermo Fisher). The same validated microsatellite-stable patient served as control ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with Hoechst 33342 (1:2000; Thermo Fisher). Positive gates were set according to unstained or isotype controls and data was analyzed by FlowJo.
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bioRxiv - Cancer Biology 2024Quote: ... Biozym Scientific GmbH) was used and analysis was done on a ViiA 7 Real-Time PCR System (4453536, Thermo Fisher Scientific). mRNA levels were normalized to the geometric mean of Cyclophilin A and hypoxanthine guanine phosphoribosyl transferase and/or 18S ...
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bioRxiv - Cancer Biology 2024Quote: Cells were plated on glass CC2-coated chamber slides (Thermo Fisher) or fibronectin-coated glass bottom dishes (Fluorodish ...
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bioRxiv - Cancer Biology 2024Quote: ... and RMA cells were grown in vitro in DMEM (Gibco 11965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 2× NuPAGE LDS sample buffer (Invitrogen), and incubated at 95 °C in a heat block for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... These cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco), supplemented with 9% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% L-glutamine (Gibco), and 1% penicillin–streptomycin (Gemini) ...
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bioRxiv - Cancer Biology 2024Quote: ... These entry vectors were then subsequently combined with entry vectors containing the pol2 promoter and Flag/Myc-tag into a custom lentiviral destination vector pDest-659 (a gift from Dominic Esposito, NCI) using Gateway LR Clonase (Thermo Fisher Scientific) to generate the final Nat10 overexpression vector ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were sub-cultured when 80% confluent using trypsin/EDTA (#15400054, Gibco-ThermoFisher) and frozen at 106 cells/vial in 10% DMSO (#D4540 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were sub-cultured when 80% confluent using trypsin/EDTA (#15400054, Gibco-ThermoFisher) and frozen at 106 cells/vial in 10% DMSO (#D4540 ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor Plus 647 (dilution 1:1000; A32728, Invitrogen), donkey anti-Goat IgG 488 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell line grew in RPMI-1640 (Gibco, Grand Island, NY) medium supplemented with 10 mM of 2-(4-(2-hydroxyethyl ...
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bioRxiv - Cancer Biology 2024Quote: Tandem Mass Tag (TMT)-10 labeling of peptides was conducted as follows: The TMT-10 plex Isobaric Label Reagent (Catalog No. 90110, Thermo Fisher Scientific, Waltham, MA) was dissolved in anhydrous acetonitrile (ACN ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10% fetal bovine serum (Gibco, Grand Island, NY). Cells were cultured at 37 °C and 5% CO2 and 95% humidity in the atmosphere.
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 647 (dilution 1:1000; A32795: Invitrogen), streptavidin Alexa Fluor 488 (dilution 1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor Plus 488 (dilution 1:1000; A32814; Invitrogen); donkey anti-Rabbit IgG 647 ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Snail (1:100, #14-9859-80 Invitrogen). Used secondary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10% fetal bovine serum (#16000044, Gibco-ThermoFisher). Cells were sub-cultured when 80% confluent using trypsin/EDTA (#15400054 ...
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bioRxiv - Cancer Biology 2024Quote: SK-N-BE(2) and NB16 cells were seeded into 8-champer slides (Thermo Fisher Scientific, 154534) with a density of 6×103 cells/well overnight and treated with indicated treatment for 72h ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, #D1306, Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... streptavidin Alexa Fluor 488 (dilution 1:100; S11223; Invitrogen). Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor Plus 488 (dilution 1:1000; A32731, Invitrogen), goat anti-mouse IgG 647 ...
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bioRxiv - Cancer Biology 2024Quote: ... using Taqman Gene Expression Assays (Applied Biosystems). Gene expression levels were normalized to 18S rRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 μg/mL streptomycin (Catalog No. 15140-122, Gibco), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with Alexa Fluor 594-conjugated anti-rabbit secondary antibody (Life Technologies) at RT for 1 h followed by staining with DAPI (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... LC-MS/MS analysis was conducted using a Nano EASY-nLC 1200 liquid chromatography system LC instrument coupled with a Q Exactive HF-X high-resolution mass spectrometer (Catalog No. BR64966, Thermo Fisher Scientific). After desalting with Ziptip columns ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mM sodium pyruvate (# 11330032, Gibco-ThermoFisher) and 10% fetal bovine serum (#16000044 ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, #D1306, Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... and 10% fetal bovine serum (#16000044, Gibco-ThermoFisher). Cells were sub-cultured when 80% confluent using trypsin/EDTA (#15400054 ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 and DLD-1 cells were cultured in DMEM (Catalog No. C11995-065, Gibco, Grand Island, NY) and RPMI 1640 (Catalog No ...
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bioRxiv - Cancer Biology 2024Quote: ... LC-MS/MS analysis was conducted using a Nano EASY-nLC 1200 liquid chromatography system LC instrument coupled with an Orbitrap Exploris 480 mass spectrometer (Catalog No. BRE725539, Thermo Fisher Scientific). After desalting with a Ziptip columns ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 546 (dilution 1:1000; Invitrogen). Nuclear counterstaining was performed using DAPI (4’,6-Diamidino-2-Phenylindole ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was quantified by a Qubit® DNA Assay Kit in Qubit® 2.0 Fluorometer (Catalog No. Q32866, Thermo Fisher Scientific). A total amount of 0.6 µg genomic DNA per sample was used as input material for the DNA sample preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... and all candidate genes were performed using the SYBR® Green PCR Master Mix (Applied Biosystems, Carlsbad CA, USA) in combination with corresponding oligonucleotide primers (Supplementary Table 1 and 2).
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mM sodium pyruvate (# 11330032, Gibco-ThermoFisher) and 10% fetal bovine serum (#16000044 ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR amplification was performed on the Prism 7900 Sequence Detection System (Applied Biosystems) using Taqman Gene Expression Assays (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... and RPMI 1640 (Catalog No. 10270-106, Gibco) with 10% FBS (Catalog No ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg/mL transferrin (MilliporeSigma, T8158), 5 ng/mL sodium selenite (MilliporeSigma, S5261) and 100 units/mL penicillin-streptomycin solution (Gibco, 15140122). Human ovarian cancer NIH:OVCAR3 (OVCAR3 ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated from omental tumors after tissues were collected and homogenized in TRIzol Reagent (Invitrogen,15596018). Semi-quantitative real-time RT-PCR was carried out using the Applied Biosystems 7500 Real Time PCR or Bio-Rad CFX Opus 96 Real-Time PCR System with the primer sequences listed below (Table 2) ...