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Citations for Thermo Fisher :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 10 mmol/L HEPES (Invitrogen, 15630-080), 2mM Glutamax (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and β-mercaptoethanol (Thermo Fisher Scientific, 21985023) before boiling for 5 min at 95 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2mM Glutamax (Invitrogen, 35050-061), 1× N2 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were washed in PBST (0.1% Tween 20, Thermo Fisher Scientific #BP337500) for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were transferred onto nitrocellulose membrane (Thermo Fisher Scientific, 88018) at 100 V for 90 min at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated with Proteinase K (Invitrogen #25530049) diluted 1:1000 in PBS for 30 min at 37°C in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: HDT livers were collected and homogenized in T-PER Tissue Protein Extraction Reagent (Life Technologies #78510) supplemented with protease (ApexBio #K1007 ...
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bioRxiv - Cancer Biology 2024Quote: ... EdU signal was detected in frozen sections using the Click-iT EdU Alexa Fluor 488 Imaging Kit (Life Technologies #C10337) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... penicillin/streptomycin (Invitrogen, 15140- 122), 10 mmol/L HEPES (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The perfused hepatocytes were gently washed and resuspended in 25 mL Hepatocyte (1X) Wash Buffer (Gibco #17704024) and centrifuged at 50g for 5 minutes at 4°C The supernatant was discarded and the wash step was repeated two additional times ...
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bioRxiv - Cancer Biology 2024Quote: ... The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen, 25300054) to obtain single cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Gateway sites were added to the cDNA through PCR and genes of interest were then used to replace the CcdB gene by Gateway recombination (Invitrogen #11789100 and #11791100). Male FVB or C57BL/6J mice were injected at approximately 6 weeks of age when their body weight was at least 20 g ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were stained with DAPI (Thermo Fisher Scientific, 62248) and mounted.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 50 ng/ml mouse recombinant EGF (Invitrogen, PMG8043). The organoids were trypsinized with 0.05% Trypsin-EDTA (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Fragments of frozen tumors were homogenized in TRIzol (TRIzol Plus RNA Purification Kit, Life Technologies #12183555) using a tissue homogenizer ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated in the secondary antibody anti-Rabbit Alex 488 (Invitrogen, A32731) at RT for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... on-column DNAse treatment was performed with PureLink™ DNase Set (Invitrogen #12185010) according to the manufacturer’s protocol (page 63 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was obtained using the SuperScript III First- Strand Synthesis System (Invitrogen, 18080-051). qPCR analyses were performed with primers listed below using SsoAdvanced Universal SYBR Green Supermix (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 μm sections were mounted onto glass microscope slides and incubated with 10 μM dihydroethidium (DHE, Thermo Fisher Scientific #50850563) at 37°C for 30 min as previously described 62 ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured in basal culture medium (advanced Dulbecco’s modified Eagle medium/F12 (Invitrogen, 12634-028), penicillin/streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed with TRIzol reagent (Invitrogen, 15596-026). Total RNA was extracted by chloroform and isopropanol precipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the livers were transferred into a sucrose (30% w/v) in PBS (Thermo Fisher Scientific #SH3002802) solution and shaken overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and lysed in RIPA buffer (Thermo Fisher Scientific #PI89900) supplemented with protease and phosphatase inhibitor tablets (Life Technologies #A32961) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1× N2 (Invitrogen, 17502-048), 1× B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... SYTOX green (0.5 µM, Thermo Scientific), an impermeable nucleic acid stain ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated with 500 nM MitoSOX Red (Invitrogen M36008) and 100 nM MitoTracker Green (Invitrogen M7514 ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells counted by Trypan blue (Gibco) exclusion at 6 and 24 hours.
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bioRxiv - Cancer Biology 2024Quote: ... and 10X NuPAGE Sample Reducing Agent (Invitrogen), followed by boiling at 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... together with PageRuler Plus prestained protein ladder (cat. no. 26619, Thermo Fisher Scientific) and transferred to a nitrocellulose membrane (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... acquired from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and 50 μg of Dynabeads Protein G (Invitrogen). After overnight incubation on a rotator at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The chemiluminescent signals were acquired by incubating the membrane with SuperSignal West Femto Maximum Sensitivity Substrate (cat. no. 34094, Thermo Fisher Scientific) and captured by ChemiDoc MP Imaging System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Mouse prostates were isolated and fixed in 10% neutral-buffered formalin (SF100-4, Thermo Fisher Scientific, Waltham, MA) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed on an ABI PRISM 7900HT Fast Real-Time PCR machine using SYBR Green (Applied Biosystems, Foster City, CA). Data was analyzed with SDS 2.3 software ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA levels were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) using the following PCR primers on a ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). Primer sequences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% penicillin-streptomycin (Life Technologies, Grand Island, NY). Cells were regularly tested for mycoplasma with MycoAlert per manufacturer’s protocol (LT07-318 ...
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bioRxiv - Cancer Biology 2024Quote: ... were stained with 1µM (MOLM-13 and MV-4-11) or 3µM (HL-60) CellTrace Far Red (Thermo Fisher Scientific) to monitor cell divisions ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse (31430, Thermo Fisher Scientific, Rockford, IL) or anti-rabbit (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Untreated cells (UNT) were given an equal volume of 0.1% DMSO in UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific) and served as a negative control ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein (40-50µg) were denatured and separated on 12% Bis-Tris SDS–polyacrylamide electrophoresis gels (NP0341BOX, Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... A Shandon Citadel 2000 Processor (Thermo Fisher Scientific) was used to process tissue on a 4-hour cycle with the following reagent cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Retrovirus was generated by transfecting oncogenic plasmids and viral packaging plasmid (pCL-Ampho) into phoenix cells with Lipofectamine 2000 (Life Technologies, Grand Island, NY). Viral particles were incubated with prostate cells (70% confluence ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 4mM GlutaMAX (Thermo Fisher Scientific) and 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse tail DNA was isolated and genotyped to evaluate transgene transmission with Phire Animal Tissue Direct PCR (F-140, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20% fetal bovine serum (FBS) (Thermo Fisher, #10437028), 5% penicillin streptomycin solution (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Cancer Biology 2024Quote: Tissues and cell protein lysates were prepared with RIPA buffer (89900, Thermo Fisher Scientific, Waltham, MA), which includes a protease inhibitor cocktail (05892791001 ...
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bioRxiv - Cancer Biology 2024Quote: ... in Tris-Buffered Saline–Tween (77500, Affymetrix, Thermo Fisher Scientific, Waltham, MA) and incubated ...
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bioRxiv - Cancer Biology 2024Quote: ... in Tris-Buffered Saline–Tween (77500, Affymetrix, Thermo Fisher Scientific, Waltham, MA) and incubated ...