Labshake search
Citations for Thermo Fisher :
451 - 500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10 % fetal bovine serum (Life technologies) and 2 % penicillin/streptomycin (Life technologies).
-
bioRxiv - Neuroscience 2024Quote: ... samples were mounted in Fluoromount (Thermo Fisher Scientific) onto glass slides with 1.5 coverslips and imaged with a Leica STELLARIS 8 confocal microscope ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA samples were quantified using a Nanodrop spectrophotometer (ThermoFisher). 10 μL first-strand cDNA was synthesized using 500 ng RNA with iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... DNA quantity and quality were measured with a NanoDrop 2000 photometer (Thermo Fisher Scientific, USA), and DNA integrity was determined by 1% agarose gel electrophoresis ...
-
bioRxiv - Neuroscience 2024Quote: ... The library was then sequenced on a S5 IonTorrent platform (ThermoFisher). FastQ files were analysed using nf-core pipeline (nf-core/rnaseq v3.0) ...
-
bioRxiv - Neuroscience 2024Quote: The cells have been collected with TrypLE (ref 12605-010, Life Technologies) and DNA extracted with the Nucleospin tissue kit following the manufacturer recommendations (ref 740952 ...
-
bioRxiv - Neuroscience 2024Quote: ... and 405 goat-antimouse (Invitrogen, 1:500) against neuron-specific nuclear protein (NeuN ...
-
bioRxiv - Neuroscience 2024Quote: ... The presence of the Cas9 protein was tested using antibody against the V5 tag (ref R960-25, Invitrogen). Secondary antibodies used for Western blot were labelled with HRP (Jackson Immuno Research) ...
-
bioRxiv - Neuroscience 2024Quote: ... mouse anti-GFP antibody (1:500; Thermo Fisher Scientific), anti-HA rMs-IgG1 (1:500 ...
-
bioRxiv - Plant Biology 2024Quote: ... The flow rate of the mobile phase was set at 0.5 mL / min and consisted of a 0.1% formic acid solution (A) (A117-50, Thermo Fisher Scientific, Waltham, MA, USA) and a 0.1% formic acid in acetonitrile (B ...
-
bioRxiv - Plant Biology 2024Quote: ... using TRIzol (Thermo Fisher Scientific). The RNA quality of the samples was checked with a 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using TRIzol (Thermo Fisher Scientific). The removal of contaminating DNA ...
-
bioRxiv - Neuroscience 2024Quote: ... and GFP (A-11122; Thermofisher). Secondary antibodies include biotinylated goat-anti rabbit and goat-anti mouse (Vector labs).
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were measured in triton-soluble fractions and in RIPA-soluble fractions using a Pierce BCA protein assay following the manufacturer’s instructions (Thermofisher Scientific).
-
bioRxiv - Immunology 2024Quote: ... peptide mixtures were analyzed with an EASY-nLC 1000 coupled to a Orbitrap Exploris 480 (ThermoFisher Scientific). 300ng of peptides were separated on 50 cm in-house-made 75 µm inner diameter columns ...
-
bioRxiv - Immunology 2024Quote: For BMDMs and GMPs RNA was extracted using Picopure RNA Isolation Kit (Thermofisher) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and quantified by using Qubit 4.0 Fluorometer (Invitrogen, Carlsbad, CA).
-
bioRxiv - Neuroscience 2024Quote: ... on day 6 and split 1:5 with a chemically defined neural medium including DMEM/F12 supplemented with GlutaMAX (1/1; Thermo-Fisher Scientific; Cat. No. 35050-061), Neurobasal medium (1/1 ...
-
bioRxiv - Neuroscience 2024Quote: ... pSer202/pThr205-Tau (AT8) (MN1020; Thermofisher) and GFP (A-11122 ...
-
bioRxiv - Neuroscience 2024Quote: ... using Restore Westernblot Stripping Buffer (Thermofisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... and Dynamin-1 (PA1-660; Thermofisher Scientific).
-
bioRxiv - Neuroscience 2024Quote: ... B27 (0.5X; Thermo-Fisher Scientific; Cat. No. 17502-048), ascorbic acid (100 μM ...
-
bioRxiv - Neuroscience 2024Quote: ... antibiotic-antimycotic (1X; Thermo-Fisher Scientific; Cat. No. 15240-062), supplemented with RA (0.1 or 1 µM ...
-
bioRxiv - Neuroscience 2024Quote: ... Cell suspensions were washed twice and resuspended in 1 mL of Dulbecco’s PBS (D-PBS) before viability staining using Live/Dead™ Fixable Aqua (1/1000; ThermoFisher) according to manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were purchased from Thermofisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... Neurobasal medium (1/1; Thermo-Fisher Scientific; Cat. No. 21103-049), N2 (0.5X ...
-
bioRxiv - Neuroscience 2024Quote: hiPSCs were engineered using a ePiggyBac (ePB) transposon previously electroporated using the Neon™ Transfection System (MPK5000, ThermoFisher). Electroporation parameters for each reaction of 4 × 105 cells were ...
-
bioRxiv - Neuroscience 2024Quote: ... Positive control cells were set aside during the first stages of the reprogramming procedures (fig. S1, Cat Nº A16517, ThermoFisher).
-
bioRxiv - Plant Biology 2024Quote: ... using the KpnI and MauBI restriction enzymes (Thermo Scientific™, ER0522 and ER2081 respectfully). The JMJ13 catalytic domain was amplified with the primers listed in Table S1 and cloned into SunTag dCas9 plasmid using the BsiWI restriction enzyme (Thermo Scientific™ ...
-
bioRxiv - Plant Biology 2024Quote: ... after DNase treatment (Invitrogen). The primers employed for Q-PCR amplification are listed in Table S4 ...
-
bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from frozen tissue using TRIZOL (ThermoFisher Scientific). Reverse transcription was conducted with 1 µg total RNA with the Superscript II enzyme (Invitrogen) ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR fragments were introduced into pDONR207 vector (Invitrogen) by BP recombination and transferred by LR recombination into destination vectors (Table S3) ...
-
bioRxiv - Plant Biology 2024Quote: ... Nitrogen (N) and carbon (C) contents were determined using an elemental analyzer (Thermoflash 2000; Thermo Scientific).
-
bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was conducted with 1 µg total RNA with the Superscript II enzyme (Invitrogen), after DNase treatment (Invitrogen) ...
-
bioRxiv - Plant Biology 2024Quote: ... A field emission scanning electron microscope (FESEM) Apreo 2S (Thermo Fisher Scientific, New Delhi, India) was used to investigate the surface morphology of solid urea and lyophilized nanourea ...
-
bioRxiv - Neuroscience 2024Quote: ... University of Iowa) and Alexa 488 anti-mouse IgG (1:1000, Molecular Probes). Confocal stacks were acquired with a 1µm slice thickness using an Olympus FV500 laser scanning confocal microscope and processed using ImageJ.
-
bioRxiv - Neuroscience 2024Quote: ... or Alexa Fluor 647 (1/1,000, Invitrogen; Burlington, ON, Canada). For quantification ...
-
bioRxiv - Neuroscience 2024Quote: ... brain-derived neurotrophic factor (10 ng/mL; Thermo-Fisher Scientific; Cat. No. PHC7074) and ciliary neurotrophic factor (10 ng/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... Fibroblast cells were incubated with CM containing 1 mM BODIPY 558/568 C12 (Red C12, Life Technologies) for 16 hr ...
-
bioRxiv - Neuroscience 2024Quote: ... BCA protein assay kit (23227) was purchased from ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... GFP (Thermo Fisher Scientific # A10262). Secondary antibodies were as follows ...
-
bioRxiv - Neuroscience 2024Quote: ... LDs were labeled with 200ng/mL BODIPY 493/503 (Life Technologies) immediately prior to imaging and was present during imaging.
-
bioRxiv - Neuroscience 2024Quote: ... followed by goat anti-rabbit HRP-conjugated secondary antibody (31466, ThermoFisher Scientific). Clarity ECL Western Blotting Substrate was used to generate chemiluminescence signals ...
-
bioRxiv - Neuroscience 2024Quote: ... anti-rabbit (Invitrogen #A31573; 1:250), and anti-mouse (Invitrogen #A21050 ...
-
bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were washed three times and incubated for 2h with secondary antibodies AlexaFluor488 or AlexaFluor647 (Life Technologies) and chromatin stained with DAPI (1μg/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... 1mM GlutaMAX (35050038, Gibco), 05 mM L-Carnitine (C0158 ...
-
bioRxiv - Neuroscience 2024Quote: ... WT and homozygous PINK1Q456X mutant fibroblasts were seeded in 96-well imaging plates (Fisher Scientific, 08772225) and allowed to attach overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... at 1 µg/ml and with HSP60 (arigo Biolaboratories, ARG10757, 1:2,000) followed by incubation with secondary antibody (Invitrogen, A-11034 and A-11041; 1:1,000) and Hoechst 33342 (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... and Hoechst 33342 (Invitrogen, H21492; 1:5,000). Plates were imaged using an Operetta CLS High Content imaging system (Perkin Elmer ...