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Citations for Thermo Fisher :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and 400 μL of n-hexane (Acros Organics; 160780010) in a capped mass spec autosampler vial (Microliter ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to assess sample quality and cDNA concentrations were quantified using a Qubit 2.0 Fluorometer with the dsDNA-High Sensitivity kit (Life Technologies Inc., Carlsbad, CA), yielding mean cDNA concentration of 1ng/ul ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA oligos (Table S2) were ordered to IDT (Coralville, Iowa, USA) and polymerase enzymes used for DNA amplification were Phusion (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were resuspended in PBS (pH 7.4, Gibco) and then subjected to five freeze and thaw cycles by alternating between liquid nitrogen and a 37 °C water bath ...
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bioRxiv - Synthetic Biology 2024Quote: ... glass bottom plates were coated overnight by diluting human laminin (ThermoFisher #A29249) to a surface density of 0.5 µg/cm2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... into a 96-well round bottom plate (Fisher Scientific #08-772-17). This plate was then analyzed with a CytoFLEX flow cytometer.
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bioRxiv - Synthetic Biology 2024Quote: ... cells washed with 100 μl PBS (pH 7.4, Gibco) per well and then 25 μl PBS and 25 μl of the Nano-Glo® Luciferase Assay System reagent (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10 % fetal bovine serum (Gibco), 4.5 g/L D-glucose ...
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bioRxiv - Synthetic Biology 2024Quote: ... for flow cytometry or MatTek No 1.5 uncoated glass bottom plates (Fisher Scientific #NC9988706) for smFISH imaging experiments ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with GlutaMAX™ (Gibco #35050087) and 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Plates were sealed (Thermo Fisher #AB0558) and briefly spun down at 500 xg for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... RNA purity and yield were assessed on a Nanodrop (Thermo Fisher), and 1-1.5 µg RNA was used a template to make cDNA with the iScriptTM cDNA synthesis kit (Bio-rad #1708890) ...
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bioRxiv - Systems Biology 2024Quote: ... The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) to obtain 10 different fractions ...
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bioRxiv - Systems Biology 2024Quote: LC-MS/MS analysis was performed using a Dionex Ultimate 3000 nano-ultra high pressure reversed-phase chromatography system coupled on-line to a Q Exactive High Field (HF) mass spectrometer (Thermo Scientific). Samples were separated on an EASY-Spray PepMap RSLC C18 column (500 mm x 75 µm ...
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bioRxiv - Systems Biology 2024Quote: ... 100 μg peptide mixture for each sample was labeled using TMT reagent according to the instructions (Thermo Scientific). The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... cells were transfected with Lipofectamine 2000 (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... Peptides were trapped on a reverse phase trap column (Thermo Scientific Acclaim PepMap100 ...
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bioRxiv - Systems Biology 2024Quote: ... 15 cm × 150 μM internal diameter) by a Dionex RSLCnano ultra-performance liquid chromatography (UPLC) system (ThermoFisher Scientific). Buffer A comprised water with 0.1% (vol/vol ...
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bioRxiv - Systems Biology 2024Quote: Samples were digested in a 96-well format using the SMART Digest kit provided by Thermo Fisher Scientific ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and 10ng/ml EGF (Fisher Scientific, Cat# 236EG200) supplemented with 5uM XAV ...
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bioRxiv - Systems Biology 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2024Quote: ... and SuperScript double-stranded cDNA synthesis kit (Invitrogen, CA). To synthesize the second strand of cDNA ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were separated on an EASY-Spray PepMap RSLC C18 column (500 mm x 75 µm, 2 µm particle size; Thermo Scientific) over a 60 min gradient of 2-35 % acetonitrile in 5 % DMSO ...
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bioRxiv - Systems Biology 2024Quote: ... and 100 U/ml penicillin and 0.1 mg/ml streptomycin (ThermoFisher, UK). Cells were maintained at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... washed twice with ice-cold PBS (Gibco) supplemented with 0.5% BSA (Bovine serum albumin ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA (Fisher scientific), and 0.2 μg/ml DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.2 μg/ml DAPI (Thermo Fisher Scientific). Stained cells were analyzed using Agilent Quanteon flow cytometer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were arrested at mitosis with colcemid (0.1 ug/mL, ThermoFisher) for 1 hour ...
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bioRxiv - Systems Biology 2024Quote: LC-MS/MS analysis was performed using a Thermo Scientific Q Exactive mass spectrometer equipped with the Easy nLC system (Proxeon Biosystems, Thermo Fisher Scientific). Peptides were trapped on a reverse phase trap column (Thermo Scientific Acclaim PepMap100 ...
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bioRxiv - Systems Biology 2024Quote: ... Labeling was performed using TMTpro 16-plex kits (ThermoFisher 44520). Each batch included one TMT channel with a labeled GIS standard ...
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bioRxiv - Systems Biology 2024Quote: ... pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher). Tissues were added to the lysis buffer immediately after excision in Rino sample tubes (NextAdvance ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... on Matrigel (Fisher Scientific, Cat#08774552) coated tissue culture plates.
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bioRxiv - Systems Biology 2024Quote: ... Peptides were monitored on a Orbitrap Eclipse mass spectrometer with high-field asymmetric waveform ion mobility spectrometry (FAIMS) (FAIMS Pro Interface, ThermoFisher Scientific). Two compensation voltages were chosen for FAIMS ...
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bioRxiv - Systems Biology 2024Quote: ... 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM)/Hams F-12 nutrient mix (F12) GlutaMAXTM media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2024Quote: ... nitric acid (conc.) were purchased from Fisher Scientific, Schwerte ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Systems Biology 2024Quote: MS raw data were searched against the UniProtKB human sequence data base (92,954 entries) and TMT 10plex quantitation performed using Proteome Discoverer software (v 2.3; Thermo Scientific). Search parameters were set to include carbamidomethyl (C ...
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bioRxiv - Systems Biology 2024Quote: ... The 44 Emory samples were randomized by age and diagnosis into 3 batches and labeled using TMTpro 16-plex kits (ThermoFisher 44520). Each batch included one TMT channel with a labeled GIS standard ...
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bioRxiv - Systems Biology 2024Quote: ... The reference samples were imaged using an AP-SMALDI5 AF ion source (TransMIT, Giessen, Germany) coupled to a QExactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). Instrument parameters are listed in SI Table 6 ...
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bioRxiv - Systems Biology 2024Quote: ... and separated on the C18-reversed phase analytical column (Thermo Scientific Easy Column ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Specifically, NPCs were plated onto Poly-L-Ornithine (PLO)(MilliporeSigma, Cat#27378-49-0),laminin (Fisher Scientific, Cat#23017015), and fibronectin (MilliporeSigma ...
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bioRxiv - Systems Biology 2024Quote: ... Peptide eluents were separated on a custom in-house packed Charged Surface Hybrid (CSH) column (1.7 um, 15 cm × 150 μM internal diameter) by a Dionex RSLCnano running capillary flow UPLC (ThermoFisher Scientific). Buffer A comprised water with 0.1% (vol/vol ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then blocked in SuperBlock (TBS) blocking buffer (Thermo Fisher Scientific) and incubated with validated primary antibodies (1:250 ...
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bioRxiv - Cancer Biology 2024Quote: ... and A2058 were maintained in DMEM (#21969, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... and KASUMI1 cells (ATCC) were grown in RPMI with 10% dialyzed fetal bovine serum (Gibco), 4mM glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... Lenti-X 293T cells (ATCC) were grown in DMEM with 10% heat inactivated fetal bovine serum (Gibco) without antibiotics ...
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bioRxiv - Cancer Biology 2024Quote: ... 4mM glutamine (Thermo Fisher; #25300164), and 1% penicillin/streptomycin (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 U/mL penicillin/streptomycin (Fisher Scientific, 15-140-122) and were passaged every 2 days ...
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bioRxiv - Cancer Biology 2024Quote: ... 80 µL of warm (CTS™ OpTmizer™ T Cell Expansion SFM, Gibco™) or RPMI 1640 media (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were stimulated with CD3/CD28 Dynabeads (11131-D; Thermo Fisher Scientific) according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells ...