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Citations for Thermo Fisher :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... RNase-free with corresponding buffer (EN0521, Thermo Fisher Scientific) and 20 U RiboLock RNase Inhibitor (EO0381 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.35 mM dNTPs (R0182, Thermo Fisher Scientific), and 35 U/mL Taq polymerase (1800 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies Alexa-fluor 488 Donkey Anti-mouse were from Fisher Scientific (A-21202), IRDye® 800CW and IRDye® 680CW were from LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... the GeneArt Gene Synthesis service (Thermo Fisher Scientific) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg total RNA was incubated with 6.25 µM oligo-dT (Metabion) and 1.875 µM random nonamer primers (Metabion) as well as 0.625 mM dNTPs (R0182, Thermo Fisher Scientific) at 70 °C for 5 min for primer annealing ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured using a BCA protein assay kit (23227, Thermo Fisher Scientific). Equal protein amounts of each sample were boiled in Laemmli buffer at 95 °C for 5 min and separated by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 U RiboLock RNase Inhibitor (EO0381, Thermo Fisher Scientific) at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.43x concentrated SYBR Green (S7567, Life Technologies), 0.35 mM dNTPs (R0182 ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were mounted with Prolong Gold Antifade mountant (Life Technologies). Images were taken at x63 magnification using a Zeiss LSM510 confocal microscope or at 40x or 60x magnification using a Nikon A1 inverted confocal with spectral detector or a Nikon Inverted spinning disk confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... or Alexa-Fluor647- conjugated secondary antibodies and Alexa-Fluor 633 Phalloidin were from Life Technologies. Coverslips were mounted with Prolong Gold Antifade mountant (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... 72 h after siRNA transfection the medium was changed to FluoroBrite DMEM Media (Thermo Fisher Scientific) with 10% serum ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dry-blotting onto nitrocellulose iBlot®gel transfer stacks using iBlot Gel transfer device (Thermo Fisher Scientific) for 7 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Supersignal West Femto Maximum Sensitivity substrate (Thermo Scientific). Equal loading was verified using an anti-GAPDH antibody (MA5-15738 ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal loading was verified using an anti-GAPDH antibody (MA5-15738, Invitrogen) after mild stripping of the membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and following probes and primers on a 7300 Real-Time PCR System (Applied Biosystems, CA). Primer/probes for mouse TNF-a gene were purchased from Applied Bioscience (Mm00443258_m1) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesised using an M-MLV Reverse Transcriptase kit (Invitrogen) and 1000 ng RNA was used ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1hr with the secondary antibody in the dark (anti-rabbit Alexa Fluor 594; ThermoFisher Cat #11012). After washing with PBS as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... 4B2-protein A/G agarose and SMP14-protein A/G agarose were prepared in siliconized 1.5ml microfuge tubes by incubating 60μg antibody with 240μl Protein A/G agarose beads (Fisher Scientific cat# IP1010ML) in a final volume of 1.2 ml of 1M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... a cell suspension of GBM cells was electroporated using a Neon transfection system (Invitrogen) with the following parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... and the libraries were quantified using Qubit kit (Thermofisher). Subsequent paired-end sequencing of cDNA libraries for RNA-Seq and ATAC-Seq were performed using HiSeq PE150 sequencing platform (Novogene).
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bioRxiv - Cancer Biology 2024Quote: ... All culture media were supplemented with antibiotics (penicillin and streptomycin, Meilunbio) and 10% Fetal Bovine Serum (FBS, Gibco). For experiments involving AR activity ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of the RNA samples were measured by NanoDrop 1000 (Thermo Fisher Scientific). RNA was reverse transcribed into cDNA using the RevertAid RT Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... the culture was washed with TBS and stained with SYTOX-Green (1:30,000, Thermo Fisher Scientific) and Hoechst-33342 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... B-27 supplement (Thermo Fisher Scientific), 10 μM Y-27632 (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from TLCV2-Ex6 transduced KMS-11 and MM1.144 cells using phenol-chloroform extraction and treatment with TURBO DNAfree kit (Ambion), followed by rRNA removal using Ribo-Zero (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... and C4-2R cells were cultured in RPMI1640 (Thermo Fisher Scientific). All culture media were supplemented with antibiotics (penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... or 400 μg/ml G418 (Thermo Fisher Scientific). Resistant colonies were selected and subcloned by limiting dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... and analyzed in single shots in the timsTOF HT mass spectrometer (Bruker Daltonics, Billerica, MA, USA) coupled to a Vanquish Neo liquid chromatography system (Thermo Fisher Scientific): 500 ng of peptides were loaded on a 45 cm HPLC column packed with 1.9 µM C18 ReproSil Pure-AQ particles (Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... and 293T cells were cultured in DMEM (Thermo Fisher Scientific), while 22Rv1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Hoechst-33342 (1:1000, Thermo Fisher Scientific) for 20 min and imaged using the AxioObserver Z1 microscope (Carl Zeiss) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 KMS-11 cells were labelled with 5 µM CellTrace™ Blue dye (Invitrogen) for 20 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: The clinical tissue specimens were digested into single cells with 0.5 mg/ml Collagenase Type IV (Thermo Fisher Scientific) in DMEM containing 25 mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... for 30 min with either Alexa Fluor 594 or 647-Azide (Invitrogen; A10270 and A10277 respectively) under click chemistry reaction conditions (100µl/well 4µM Azide compound ...
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bioRxiv - Cancer Biology 2024Quote: ... cells grown in 12-well glass bottom plates were incubated with 20µM EdU added directly to the culture medium (Invitrogen; cat# A10044) for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 7.4) supplemented with Pierce CompleteTM protease inhibitor cocktail tablet (ThermoFisher Cat# A32961) as specified by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-CD34 (1:1000) (MA1-10202, Invitrogen) along with Rabbit isotype control IgG (1:2500 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in 5% MOPS SDS Running Buffer (Thermo Fisher Scientific), followed by dry-blotting onto nitrocellulose iBlot®gel transfer stacks using iBlot Gel transfer device (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... purified SLBR-N was labeled with Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific) per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10 % fetal bovine serum and 1 % mixture of antibiotics penicillin-streptomycin (all from Gibco, Waltham, MA, USA) at 5 % CO2 and 37 °C in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100µg/mL streptomycin (Life Technologies, UK) at 37 °C in a humidified atmosphere at 5 % CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Substrate-induced changes of cell proliferation were analyzed after 5 days of culture using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with DAPI and Alexa Fluor 488 phalloidin (Thermo Fisher Scientific). Images were captured as z-stacks with a 5 µm-step size using a confocal microscope (710 LSM ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counted using Countess II FL Automated Cell Counter (ThermoFisher, Waltham, MA, USA) and the 3-6 x 107/ml cell stock was prepared for further experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were analyzed on a Dionex Ultimate 3000RS liquid chromatography system coupled to a TSQ Quantiva mass spectrometer (Thermo Scientific) using electrospray ionization in switching polarity mode with the following ion source parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% penicillin/streptomycin (Gibco). MR42D and MR49F (hormone resistant ...
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bioRxiv - Cancer Biology 2024Quote: ... NETs were quantified by collecting the supernatant and measuring cellular free DNA using Quant-iT Picogreen (Invitrogen, Grand Island, NY) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and miRNA isolation kit (Ambion). The quality of RNA was checked using Bioanalyzer RNA 6000 Nano kit (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were run on NativePAGE 3 to 12% Bis-Tris gels (Invitrogen #BN1001BOX) with NativeMark Unstained Protein Standard (Invitrogen #LC0725) ...