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Citations for Thermo Fisher :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: ... We evaluated the extracted DNA quality and quantity by agarose gel electrophoresis (1% w/v) stained with SYBR Safe DNA Gel Stain (Invitrogen) and by visual comparison with lambda DNA (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... coli (Invitrogen 12297016), followed by restriction digestion and insertion into pSM vector (78 ...
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bioRxiv - Genetics 2024Quote: ... while the starved condition was incubated in Hanks’ Balanced Salt Solution (HBSS) (Gibco #14175095) for 3 ...
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bioRxiv - Cell Biology 2024Quote: ... Penicillin-Streptomycin (100 Units/ml; 100 μg/ml respectively, ThermoFisher # 15140122), Activin A (100 ng/ml ...
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bioRxiv - Genetics 2024Quote: ... and RNAseOUT (Invitrogen 10777-019).
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bioRxiv - Genetics 2024Quote: ... Worms were starved at 20°C while being continuously rotated on a tube rotator (ThermoFisher) at 20 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... TrypLE Select Enzyme solution (ThermoFisher #12563011) was introduced to the bottom channel and chips were incubated for 10 minutes at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were washed twice in DPBS (Gibco, #14190-094), re-suspended in fresh Basement Membrane Matrigel and plated in domes as described before.
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA was extracted from Lbm and Lh individuals using the TRIzol reagent (Invitrogen), and RT-PCR experiments were performed using the RacGAP1-specific degenerate primers ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM sodium pyruvate (100 mM, #11360070, Gibco), 20 mM HEPES (1 M ...
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bioRxiv - Neuroscience 2024Quote: ... 100 U/ml Penicillin/streptomycin (10,000 U/ml, #15140122, Gibco), 1 mM sodium pyruvate (100 mM ...
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bioRxiv - Neuroscience 2024Quote: ... and Glutamax-1 (100X, #35050-061, Gibco). The homogenous cells were seeded onto poly-L-lysine-coated coverslip in 12-well plates or 6-well plates ...
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bioRxiv - Neuroscience 2024Quote: ... The dissected cortices were then transferred to a 15 ml tube and washed three times using dissection buffer consisting of HBSS (10X, #14185052, Gibco), 100 U/ml Penicillin/streptomycin (10,000 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... The cortices were incubated in a dissection buffer containing 0.03% trypsin-EDTA (0.25%, #25200056, Gibco) and 50 U/ml DNase (10,000 U ...
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...
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bioRxiv - Neuroscience 2024Quote: ... and quantitative PCR was performed using Fast SYBR™ Green Master Mix Kit (Applied Biosystems, #4385612). Gapdh was used as control to normalize the expression levels of analyzed gene transcripts ...
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bioRxiv - Neuroscience 2024Quote: ... and NOD2−/− primary microglia treated with M18-MDP (1 μg/ml) or α-Syn PFF (5 μg/ml) were lysed in ubiquitination lysis buffer (#PI89900, ThermoFisher), supplemented with protease inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... 0.25% triton X-100 and 1XTBS at 4°C followed by immunostaining using goat Alexafluor-labeled secondary antibodies (Thermo Fisher Scientific or Jackson ImmunoResarch Laboraties ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Invitrogen, #11766500), and quantitative PCR was performed using Fast SYBR™ Green Master Mix Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted on glass slides using ProLong Gold (#P36934, Thermo Fisher Scientific) and imaged on a Nikon laser scanning confocal microscope or a Ti2 widefield microscope at the Northwestern University Center for Advanced Microscopy and Nikon Imaging Centre.
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bioRxiv - Neuroscience 2024Quote: ... The total protein content of the lysates was determined by BCA protein assay (#23227, ThermoFisher), and 20 µl of Anti-Ub TUBE agarose resin was added ...
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bioRxiv - Neuroscience 2024Quote: ... and developed using Pierce ECL (enhanced chemiluminesence) (Thermo Fisher Scientific). ECL signals were quantified on a Bio-Rad ChemiDoc MP Imaging System and chemiluminescent signals were quantified using Bio-Rad Image Lab Software (6.1).
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bioRxiv - Neuroscience 2024Quote: ... in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific) (2 h ...
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bioRxiv - Neuroscience 2024Quote: ... plus TRIzol (Life Technologies, #15596-026). cDNA was synthesized using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and Halt phosphatase inhibitor (#78420, Thermo Fisher Scientific). Protein concentration was determined using bicinchoninic acid assay (BCA ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were separated by 4-12% NuPAGE (Invitrogen) gels using MOPS buffer under reduced and denatured conditions ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from NPJ using the PureLink RNA Micro Kit (ThermoFisher, #12183-016) plus TRIzol (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Visualizations of the primary antibodies were achieved using suitable secondary antibodies conjugated with Alexa fluorophores (Alexa Fluor 488, 594, and 647, Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... All constructs were prepared by Genscript USA (Piscataway, NJ) and cloned into a pcDNA3.1(+) vector backbone (Thermo Fisher Scientific, Waltham, MA). Constructs were confirmed by sequencing analysis.
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bioRxiv - Neuroscience 2024Quote: ... in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific) (2 h ...
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bioRxiv - Developmental Biology 2024Quote: ... washed 3 times with PBT1% (1% Triton X-100 in PBS) and permeabilised using a 0.25% trypsin solution (Gibco) at 25°C when embryos were older than 24 hpf ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... Katna1 transcripts were reverse-transcribed and PCR-amplified using the SuperScript™ III One-Step RT-PCR System with Platinum® Taq (ThermoFisher Scientific, France) from 200 ng of katna1+/+ and katna1-/-MZ RNA extracts using the following primers:
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Zn-5 (1/200; ZIRC, University of Oregon) and GFP (1/1000; Molecular Probes). After several washes in PBT1% ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with the appropriate secondary antibody (Alexa Fluor 488 or 555 at 1/1000, Molecular Probes).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using bicinchoninic acid assay (BCA) assay (#23225, Thermo Fisher Scientific). Equal amounts of protein were separated by 4-12% NuPAGE (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and 200 µg/ml hygromycin (Life Technologies, Grand Island, NY) (37°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested using pre-mixed Earle’s Balanced Salt Solution (EBSS) with 5 mM EDTA (Life Technologies) and centrifuged at 3000 rpm (10 min ...
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bioRxiv - Neuroscience 2024Quote: ... and an autosampler coupled with a TSQ Vantage mass spectrometer (Thermo Fisher Scientific). Separation of the analyte from potentially interfering material was achieved at ambient temperature using an Agilent Eclipse Plus column (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were passaged with Versene (ThermoFisher Scientific, Waltham, MA), and transferred onto fresh Matrigel (Corning ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher] ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... 1x N2 (Life Technologies, Carlsbad, CA) and 1x B27 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... and commercially available TaqMan primers (Invitrogen) for synaptophysin (Mm00436850_m1) ...
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bioRxiv - Neuroscience 2024Quote: ... Neurotrace 435/455 (1:250, ThermoFisher N21479) was added to the secondary antibody solution ...
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bioRxiv - Neuroscience 2024Quote: RNA was isolated from the remainder of the synaptosomal preparations using Tri-Reagent (Thermo Fisher, Waltham, MA) using the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... mounted in Permount (Fisher Scientific, Pittsburg, PA) and cover slipped ...