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Citations for Thermo Fisher :
351 - 400 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and anti-CD34 (1:1000) (MA1-10202, Invitrogen) along with Rabbit isotype control IgG (1:2500 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl cDNA (1000 ng/ 100 μl) and 6 μl nuclease-free water (Thermo Scientific). qRT-PCR cycles included pre-incubation at 95°C for 5 minutes followed by 40 cycles of 95°C for 10 seconds 60°C for 30 seconds and 72°C for 30 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by electrophoresis in 5% MOPS SDS Running Buffer (Thermo Fisher Scientific), followed by dry-blotting onto nitrocellulose iBlot®gel transfer stacks using iBlot Gel transfer device (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... purified SLBR-N was labeled with Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific) per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10 % fetal bovine serum and 1 % mixture of antibiotics penicillin-streptomycin (all from Gibco, Waltham, MA, USA) at 5 % CO2 and 37 °C in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... Substrate-induced changes of cell proliferation were analyzed after 5 days of culture using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and stained with DAPI and Alexa Fluor 488 phalloidin (Thermo Fisher Scientific). Images were captured as z-stacks with a 5 µm-step size using a confocal microscope (710 LSM ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were counted using Countess II FL Automated Cell Counter (ThermoFisher, Waltham, MA, USA) and the 3-6 x 107/ml cell stock was prepared for further experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were analyzed on a Dionex Ultimate 3000RS liquid chromatography system coupled to a TSQ Quantiva mass spectrometer (Thermo Scientific) using electrospray ionization in switching polarity mode with the following ion source parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% penicillin/streptomycin (Gibco). MR42D and MR49F (hormone resistant ...
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bioRxiv - Cancer Biology 2024Quote: ... NETs were quantified by collecting the supernatant and measuring cellular free DNA using Quant-iT Picogreen (Invitrogen, Grand Island, NY) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and miRNA isolation kit (Ambion). The quality of RNA was checked using Bioanalyzer RNA 6000 Nano kit (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were run on NativePAGE 3 to 12% Bis-Tris gels (Invitrogen #BN1001BOX) with NativeMark Unstained Protein Standard (Invitrogen #LC0725) ...
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bioRxiv - Cancer Biology 2024Quote: Mitochondria were extracted with 1x sample buffer (NativePAGE Sample Prep Kit, Invitrogen #: BN2008) containing 1% digitonin for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted using RNAlater ICE (Ambion) and miRNA isolation kit (Ambion) ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using 1X Fast SYBR Green Master mix (Applied Biosystems #4385612), 400 nM primers ...
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bioRxiv - Cancer Biology 2024Quote: ... with NativeMark Unstained Protein Standard (Invitrogen #LC0725). Running buffers were prepared using the NativePAGE Running Buffer Kit (Invitrogen #BN2007) ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was determined using a Bicinchoninic Acid Protein (BCA) assay (Thermo Scientific) on a Tecan Infinity M200 microplate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10% dialyzed FBS (Gibco 26400-044). After 24 hours media was changed to the same media supplemented with 5 mM glucose (unlabeled or 13C6 labeled) ...
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bioRxiv - Cancer Biology 2024Quote: ... [1-14C]-palmitate (0.1μCi/mL) conjugated to 2% (wt/vol) FA-free BSA and 1 mmol/L -carnitine in RPMI glucose-free medium (Gibco) for 4 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×107 CFU were added into wells of 96-well high binding microplates (Thermofisher, 15041) and incubated at 4LC overnight [31] ...
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bioRxiv - Cancer Biology 2024Quote: ... Running buffers were prepared using the NativePAGE Running Buffer Kit (Invitrogen #BN2007). 1x running buffer was used as the anode buffer in a Thermo XCell SureLock Mini-Cell (#EI0001) ...
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bioRxiv - Cancer Biology 2024Quote: ... at 488 nmEx/585 nmEm while mitochondrial ROS levels were determined using the MitoSOX probe (ThermoFisher Scientific) at 488 nmEx/530nmEm ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 nM MitoTracker Green (Invitrogen M7514) or 1.5 µM CM-H2DCFDA (Invitrogen C6827 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in 500 µL lysis buffer supplemented with 1x protease and phosphatase inhibitor cocktail (PPIC, Thermo Scientific 1861281) and 10 µM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Cancer Biology 2024Quote: ... or 1.5 µM CM-H2DCFDA (Invitrogen C6827) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell cycle was determined using Vybrant DyeCycle™ Violet Stain (Thermo Fisher Scientific) at 405 nmEx/437 nmEm ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were changed to DMEM (Gibco A1443001) supplemented with 0.05 mM glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... C4-2B, 22Rv1, AD1 (AR+, androgen independent), and PC-3 (AR-, hormone resistant) cells were cultured in RPMI1640 medium (Life Technologies), supplemented with 10% FBS (Cytiva Hyclone) ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA quantity was measured using a NanoDrop spectrometer (Thermo Scientific, ND-1000). cDNA was generated using the RevertAid RT Reverse Transcription Kit (Fermentas) ...
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bioRxiv - Cancer Biology 2024Quote: ... Graphs were generated by normalizing values to the total cell number measured by the Alamar Blue™ HS Cell Viability Reagent (Thermo Scientific) and relative to the control condition.
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bioRxiv - Cancer Biology 2024Quote: ... After 24 hours the media was removed and replaced with glucose- and glutamine-free media (Gibco) supplemented with 5 mM glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... no glutamine (0 mM) media (Gibco), supplemented with 5 mM glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... using a Savant SpeedVac (Thermo Fisher) for metabolomics LC-MS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... then resuspended in PBS + 1% BSA + 0.1 µg/mL DAPI (Invitrogen D3571). The geometric mean of the fluorescent intensity was measured with a 488 nm laser and 505LP/530/30 emission filter (MitoTracker Green/CM-H2DCFDA) ...
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bioRxiv - Cancer Biology 2024Quote: ... MR42D and MR49F (hormone resistant, treatment resistant) cells were cultured in RPMI 1640 medium (Life Technologies), supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.1×150 mm (Thermo Fisher Scientific, Waltham, MA). The column was maintained at 35°C and tray at 20°C ...
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bioRxiv - Cancer Biology 2024Quote: Human ccRCC cell lines 786-O and RCC4 were carried in DMEM/F12 (Cytiva #SH30023) + 10% fetal bovine serum (Gibco #16000-044) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... no glutamine media (Gibco) supplemented with 5 mM glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the BCA protein assay kit (Thermo Fisher Scientific), and 30 µg of protein per sample was used for MS sample preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were re-suspended in 1 ml 1 µg/ml Hoechst 33342 in PBS (Invitrogen 10 mg/ml stock). Cell cycle analysis was performed using a BD Bioscience FACSCanto II flow cytometry system using a 401 nm laser ...
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bioRxiv - Cancer Biology 2024Quote: Drug sensitivity dose curves were generated using the CyQUANT NF Cell Proliferation Assay (Invitrogen C35006) using 96 well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary labelling was with NeutrAvidin-DyLight 650 (84607; Thermo Fisher Scientific) or Streptavidin-Alexa Fluor 488 (S11223 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mL Glutamax (2 mM, ThermoFisher 35050061), 5 mL Penicillin/Streptomycin (100 U/mL and 100 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 mL B-27 supplement (Gibco 17504044), 1 mL Normocin (Invivogen ant-nr-1) ...
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bioRxiv - Cancer Biology 2024Quote: ... Penicillin/Streptomycin (100 U/mL and 100 μg/mL, respectively, ThermoFisher 15140122), and Amphotericin B (0.25 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: Detached cells were fluorescently labeled for 15 minutes with 10 μM CellTracker Green CMFDA Dye (Invitrogen) in growth media ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow cytometry was performed on an Attune NxT flow cytometry (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... 5 mL Penicillin/Streptomycin (100 U/mL and 100 μg/mL, respectively, ThermoFisher 15140122), 10 mL B-27 supplement (Gibco 17504044) ...
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bioRxiv - Cancer Biology 2024Quote: ... using a 0.25 mg/mL trypsin/EDTA (ThermoFisher, Waltham, MA, USA) and resuspended in the fresh culture medium ...