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Citations for Thermo Fisher :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... using TRIzol® (Invitrogen, #15596018) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized following manufacturer’s protocol of RevertAid H minus First Strand cDNA Synthesis Kit with random hexamer primers (Thermo Scientific, #K1632). Quantitative real-time PCR was performed with iQ SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids were transfected into the cells using Lipofectamine2000 (Life Technologies 11668019). 1 µg of DNA was complexed with 3 µg of Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein extracts were diluted in Bolt™ LDS Sample Buffer (Invitrogen™; B0008). Equal amounts of protein samples were separated using BOLT pre-cast 4–12% gradient gels (Invitrogen™ ...
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bioRxiv - Cell Biology 2024Quote: ... were fixed with 4% PFA (Invitrogen, 28906) in PBS for 15min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: RPE1-hTERT cells were washed generously with ice-cold PBS prior to labelling with cell-impermeable 0.2 mg/mL Sulfo-NHS-SS Biotin (ThermoFisher Scientific, no. 21217) in PBS (pH 7.4) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10mM HEPES (Thermo Fisher) and 1μM UMK57 (if relevant ...
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bioRxiv - Cell Biology 2024Quote: ... the following secondary antibodies were incubated at RT for 1h: anti-IgG rabbit (1:1000; Alexa Fluor 488 or 546, Life Technologies, A11008 or A11010), anti-IgG mouse (1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with plasmid DNA using Lipofectamine 2000 (Thermo Scientific) for approximately 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... media was supplemented with N2 and FGF (20 ng/ml) and on day 10 EB were adhered to the surface covered with Geltrex (Invitrogen). After 4-5 days in culture ...
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bioRxiv - Cell Biology 2024Quote: ... Chemilumiscent detection of HRP-conjugated secondary antibodies was performed using Pierce ECL Western blotting substrate (Thermo Fisher Scientific; 32106) or Pierce SuperSignal West Femto Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MS analysis was performed using a Q-Exactive HF mass spectrometer (Thermo Scientific). For ionization ...
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bioRxiv - Cell Biology 2024Quote: ... 72ug of each insert dsDNA fragment was digested by ApaI (#ER1415, Thermo Fisher Scientific, US) and BglII (#ER0082 ...
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bioRxiv - Cell Biology 2024Quote: ... 8uL of Lipofectamine RNAiMAX (Thermo Fisher) was added to one tube of 1.2mL of Opti-MEM (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... anti-IgG mouse (1:1000 dilution, Alexa Fluor 546; Life Technologies, A11030), anti-IgY chicken (1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... was added to one tube of 1.2mL of Opti-MEM (Thermo Fisher) and 240pmol of Mad2L1 siRNA (Silencer Select ...
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bioRxiv - Cell Biology 2024Quote: ... non-essential amino acids (Gibco, 11140, 050), antibiotics (P/S ...
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bioRxiv - Cell Biology 2024Quote: ... and trypsin (Gibco,15400, 054) were purchased from Thermo Fisher Scientific Incorporation ...
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bioRxiv - Cell Biology 2024Quote: ... antibiotics (P/S; Gibco, 15070, 063) and trypsin (Gibco,15400 ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR data were obtained using QuantStudio 3 Real-Time PCR System and analysis was performed using Design and Analysis Software v2 (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... 90% high-glucose DMEM (Gibco,11965, 092) and 10% FBS were mixed ...
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bioRxiv - Cell Biology 2024Quote: ... running at 250 nL/min on a Proxeon EASY-nLC pump was applied to samples in-line with a hybrid LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA). A parent ion scan was performed in the Orbitrap at a 60,000 resolution (fwhm) ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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bioRxiv - Cell Biology 2024Quote: ... AF-488-conjugated transferrin was from Molecular Probes by Invitrogen Life Technologies (T13342 ...
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bioRxiv - Cell Biology 2024Quote: ... AF-488-conjugated transferrin was from Molecular Probes by Invitrogen Life Technologies (T13342, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2024Quote: ... or 647 (1:500, Molecular Probes), and DAPI (10 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... and DAPI (10 ng/ml, ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... fetal bovine serum (FBS; Gibco,10099,141C), non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA transfection was performed using reverse transfection protocols with Lipofectamine RNAiMAX transfection reagent (13778, Life technologies). For CLTC knockdown ...
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bioRxiv - Cell Biology 2024Quote: ... embryos were further blocked in BBT supplemented with 2% normal goat serum and incubated for 2 hr at room temperature with secondary antibodies and DAPI (10 ng/ml, ThermoFisher). Embryos were mounted in Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using SYBR™ Green PCR Master Mix kit (4367659, Applied Biosystems). Human GAPDH and green monkey β-actin were used as reference controls ...
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bioRxiv - Cell Biology 2024Quote: 293 T-REx Flp-In cells stably expressing Robo1-WT-BirA*-Flag or Robo1-NTM-BirA*-Flag were generated using the Flp-In system (Invitrogen, Thermo Fisher Scientific, Rockford, IL, USA) after Hygromycin B selection (Coyaud et al. ...
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bioRxiv - Cell Biology 2024Quote: ... treated with SupersignalTM Chemiluminescent substrate (Thermo Fisher Scientific, Rockford, IL, USA) and visualized on a ChemiDoc MP imaging system (Bio-Rad laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... 647 anti-rabbit (Life Technologies, Cat ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20-25 mL of the combined PDMS product was poured onto the molds placed in a petri dish (Fisher Scientific, Cat. # FB0875713A) with pins positioned at either end of the channel to create holes for tubing ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were generated using CollibriTM Stranded RNA library prep for ilIuminaTM Systems (ThermoFisher Scientific) and sequenced using HiSeq XTM Ten (Illumina ...
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bioRxiv - Cell Biology 2024Quote: Total mRNA was purified with a PureLinkTM RNA Mini Kit (Ambion, Inc.). RNA-seq libraries were generated using CollibriTM Stranded RNA library prep for ilIuminaTM Systems (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded on the plate in mediums containing 1/10.000 Hoechst 33342 (Invitrogen) and either the drug vehicle (3.5 µL DMSO/1 mL of medium ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed 3 times with TBST (10mins each) and visualization performed using ECL and ECL Prime (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-mouse 488 (1:500, Invitrogen #A-11029), donkey anti-rabbit 555 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: E14tg2a ESCs (RIKEN Cell Bank) were cultured in Glasgow minimum essential medium (GMEM; Wako) containing 10% fetal calf serum (FCS; Invitrogen), 1 mM glutamine (Wako) ...
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bioRxiv - Cell Biology 2024Quote: Cells were transfected the day before the experiment using Neon transfection device (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were generated using CollibriTM Stranded RNA library prep for ilIuminaTM Systems (ThermoFisher Scientific) and sequenced using HiSeq XTM Ten (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: Total mRNA was purified with a PureLinkTM RNA Mini Kit (Ambion, Inc.). For quantitative RT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... the medium of the cells was changed to 500µL of medium containing 1/10.000 Hoechst 33342 (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... 647 anti-mouse (Life Technologies, Cat ...
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bioRxiv - Cell Biology 2024Quote: ... Reacted blots were covered with Pierce™ ECL horseradish peroxidase substrate (ThermoFisher #32209) for 1 min at room temper-ature ...
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bioRxiv - Cell Biology 2024Quote: Cells for immunofluorescence analysis of centrosomes were fixed using pre-chilled (−20°C) methanol (ThermoFisher Scientific Cat. # A412P-4) for 10 minutes at −20°C ...
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bioRxiv - Cell Biology 2024Quote: ... and secondary Alexa Fluor-555 (ThermoFisher, goat anti-Mouse ...
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bioRxiv - Cell Biology 2024Quote: ... and Pierce Magnetic A/G beads (ThermoFisher Scientific, Cat. # 88803) used for immunoprecipitation assays were purchased from the sources detailed ...