Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... then treated with TURBO DNA-freeTM Kit (Ambion) to remove contaminating genomic and plasmid DNA ...
-
bioRxiv - Plant Biology 2024Quote: ... and the pellet was resuspended in 500 μl of 1X DPBS buffer (Gibco™. Catalog number: 14040141) with 1% BSA ...
-
bioRxiv - Plant Biology 2024Quote: ... SuperSignal West Pico Chemiluminescent Substrate kit (Thermo Scientific) was used for immunodetection ...
-
bioRxiv - Plant Biology 2024Quote: ... and the nuclei count was determined under 20X brightfield microscope using a Chemglass Life Sciences Disposable Hemocytometer (Fisher Scientific, catalog number: 50-131-1352). The nuclei count was adjusted to 3000/μL using 1X Diluted Nuclei Suspension Buffer (10X Genomics ...
-
bioRxiv - Plant Biology 2024Quote: ... we followed the HisPur Ni-NTA resin batch protocol (Thermo Scientific). Ni-NTA-bound AvrStb6 was eluted in phosphate-buffered saline (PBS ...
-
bioRxiv - Plant Biology 2024Quote: ... then subcloned using Gateway LR clonase enzyme (Invitrogen) into the pGWB2 vector (55 ...
-
bioRxiv - Plant Biology 2024Quote: ... were measured using an ultra-high performance-liquid chromatography (UHPLC)-electrospray interface quadrupole-orbitrap mass spectrometer (UHPLC/Q-Exactive; Thermo Scientific) setup equipped with an ODS column (ACQUITY UPLC HSS T3 ...
-
bioRxiv - Plant Biology 2024Quote: ... indica-infected wild-type and YUC9ox seedlings were extracted as described above and quantified using a Nanodrop ND-1000® UV/Vis spectrophotometer (ThermoFisher). RNA quality was additionally checked on a Bioanalyzer 2100 (Agilent ...
-
bioRxiv - Physiology 2024Quote: ... RT-qPCR was performed using SYBR green master mix (Applied biosystems), cDNA ...
-
bioRxiv - Physiology 2024Quote: ... with protease and phosphatase inhibitors (Thermo Fisher). Protein concentration was quantified using a Pierce BSA protein assay kit (Thermo Fisher) ...
-
bioRxiv - Physiology 2024Quote: ... Protein concentration was quantified using a Pierce BSA protein assay kit (Thermo Fisher). Protein concentrations were normalized and samples for Western blot were prepared using RIPA buffer and loading dye to a final concentration of 4x ...
-
bioRxiv - Physiology 2024Quote: ... SuperScript II RT (Invitrogen) was added to each reaction and incubated at 42°C for 50 minutes prior to enzyme inactivation by heating at 70°C for 15 minutes ...
-
bioRxiv - Physiology 2024Quote: ... dNTP mix (Thermo Scientific), and sterile water ...
-
bioRxiv - Physiology 2024Quote: ... Samples were heated at 65 ° for 6 minutes and quick chilled on ice prior to adding First strand buffer (Invitrogen) and DTT (Invitrogen ...
-
bioRxiv - Physiology 2024Quote: ... Protein was extracted using RIPA buffer (Thermo Fisher) with protease and phosphatase inhibitors (Thermo Fisher) ...
-
bioRxiv - Physiology 2024Quote: ... Slides were then incubated in 0.5% Triton X-100/PBS for 20 minutes at RT and EdU staining was performed using a Click-iT EdU Kit (Invitrogen) following the included protocol ...
-
bioRxiv - Physiology 2024Quote: ... Isopropanol (Acros Organics) was added to cause RNA precipitation before the pellet was washed in 70% ethanol and allowed to dry before being reconstituted with water ...
-
bioRxiv - Physiology 2024Quote: Samples were deparaffinized using xylene (Acros Organics) and rehydrated using washes of 100% and 95% ethanol at room temperature ...
-
bioRxiv - Physiology 2024Quote: ... Samples were homogenized in Trizol (Ambion), the supernatant removed ...
-
bioRxiv - Physiology 2024Quote: ... and DTT (Invitrogen) and incubating at 42°C for 2 minutes ...
-
bioRxiv - Physiology 2024Quote: Samples were deparaffinized using xylene (Acros Organics) and rehydrated using washes of 100% ...
-
bioRxiv - Neuroscience 2024Quote: ... LysoTracker™ Deep Red (Invitrogen L12492), TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium) ...
-
bioRxiv - Physiology 2024Quote: ... Transfection was performed using Lipofectamine CRISPRMAX transfection reagent (Invitrogen, Cat#CMAX00003). Briefly ...
-
bioRxiv - Physiology 2024Quote: ... Microspheres and PBS flush were directly aspirated from the left atrium using a 1000 µl wide bore pipette tip (ThermoFisher, 2079GPK). Microspheres were then aliquoted into a 96 well plate and quantified using a fluorescent plate reader (SpectraMax i3x ...
-
bioRxiv - Physiology 2024Quote: ... Protein concentration was measured using the BCA assay (Thermo Fisher Scientific, Waltham, MA 23225). Fifteen µg of lysate in Laemmli sample buffer was heated at 95°C for 5 minutes and loaded onto a 10% SDS-PAGE gel and ran at 115 volts for 1-1.5 hours ...
-
bioRxiv - Physiology 2024Quote: ... All blood was collected free flowing from the right femoral artery using a 30g needle and heparin coated blood gas syringe (Fisher Scientific, 22-024-978).
-
bioRxiv - Physiology 2024Quote: ... we used 10µm fluorescent microspheres (ThermoFisher, F8834) to objectively quantify intrapulmonary shunting exclusively within the left lung ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were passaged at 70-90% confluency using Versene (Gibco; 15040066), and neuralized between passages 10-24.
-
bioRxiv - Neuroscience 2024Quote: ... secondary antibody goat anti-rat AlexaFluor 568 (1:800, Thermo Fisher Scientific Inc), secondary antibody donkey anti-rabbit AlexaFluor 488 (1:500 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: The T-REx™-293 cell line was obtained from Invitrogen (CA, U.S.A). T75 and T175 mammalian cell culture flasks were purchased from Fisher scientific (Loughborough ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: The in vitro screening assay was adapted from (Duvall et al., 2019), and carried out at HD Biosciences (HDB, Shanghai) as follows: HEK293T (ThermoFisher Scientific) cells were grown in DMEM (high glucose ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... rinsed in D-PBS without Ca2+/Mg2+ (Gibco, United States) and placed in a digestion solution (collagenase 2,5U/ml in DMEM ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 1% non-essential amino acids (Gibco, Carlsbad, US) at 37°C and 5% CO2 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and Sequence Detector Software (SDS version 1.6; PE Applied Biosystems). We used the relative standard curve method for these analyses (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/cms_042380.pdf).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The two cell lines were cultured separately in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Carlsbad, US) supplemented with 10% foetal bovine serum (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Dihydrochlorid (DAPI) for 5 min (1:100, #D1306, Invitrogen, Waltham, US). Samples were mounted on glass slides and z-stacks were acquired using an LSM900 confocal laser scanning microscope (Carl Zeiss ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were incubated with a goat-anti-rabbit Alexa Fluor™ 488 antibody (1:500, #A-11008, Invitrogen, Waltham, US) for 1 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by staining with Alexa Fluor™ 546 Phalloidin for 1 h (1:400, #A22283, Invitrogen, Waltham, US) and 4’,6-Diamidin-2-phenylindol ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the absorbance of each well at 450 nm was measured using a microplate reader (Thermo Fisher Scientific, MA, USA).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100×; NEAA, Gibco, USA) at a 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 10% fetal bovine serum (FBS; Gibco, CA, USA), and 1% MEM NON-ESSENTIAL AMINO ACIDS SOLUTION (100× ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing 1% penicillin/streptomycin (P/S; Gibco, CA, USA), 10% fetal bovine serum (FBS ...
-
bioRxiv - Neuroscience 2024Quote: ... The culture medium was switched to Neurobasal medium (Life Technologies) containing N21 ...
-
bioRxiv - Neuroscience 2024Quote: ... StemFlex was supplemented with RevitaCell™ (Gibco; A2644501; StemFlex:RC hereafter) to promote cell survival ...
-
bioRxiv - Neuroscience 2024Quote: ... ProLong™ Gold Antifade Mountant with DNA Stain DAPI (Invitrogen P36931), Tissue-Tek® O.C.T ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were gently dissociated using TrpLE Express (Life Technologies), counted and collected by centrifugation ...
-
bioRxiv - Neuroscience 2024Quote: ... in 1x borate buffer (Thermo Scientific; 28341) for 4h at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... or 555 (1:800, Thermo Fisher Scientific Inc), secondary antibody donkey anti-mouse Alexa Fluor 488 (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: The LC–MS/MS measurements were performed on an Ultimate 3000 RSLC nano LC system (Dionex) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). Peptides were concentrated on a pre-column (Acclaim PepMap nanoViper ...
-
bioRxiv - Neuroscience 2024Quote: ... and goat anti-rabbit Alexa Flour 555 (1:500; Life Technologies) at room temperature for one hour ...